作者机构：
[She, M.-H.; Jiang, W.-Y.; Hu, X.-B.; Yin, W.-D.] Dept. of Biochemistry and Molecular Biology, School of Life Sciences and Technology, University of South China, Hengyang Hunan 421001, China;[Yang, S.-H.] First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China;[Laudon, M.] Drug Discovery, Neurim Pharmaceuticals Lad, Tel-Aviv, Israel
作者机构：
[Wang, P.-P.] Institute of Cardiovascular Diseases, University of South China, Hengyang Hunan 421001, China;[She, M.-H.] Dept. of Biochemistry and Molecular Biology, University of South China, Hengyang Hunan 421001, China;[Laudon, M.] Neurim Pharmaceuticals Ltd., Israel;[Yin, W.-D.] Institute of Cardiovascular Diseases, University of South China, Hengyang Hunan 421001, China, Dept. of Biochemistry and Molecular Biology, University of South China, Hengyang Hunan 421001, China
关键词：
胰岛素抵抗;甘油三酯;脂肪组织甘油三酯酶;激素敏感性脂肪酶
摘要：
目的探讨脂肪组织甘油三酯酶(adipose triglyceride lipase,ATGL)及激素敏感性脂肪酶(hormone-sensitive lipase,HSL)在褪黑素非选择性受体激动剂Neu-p11改善高糖高胰岛素(high glucose and insulin,HGI)诱导的3T3-L1脂肪细胞胰岛素抵抗(insulin resistance,IR)中的作用及机制.方法培养3T3-L1脂肪细胞,HGI诱导IR模型.以葡萄糖消耗量及细胞内甘油三酯(triglyceride,TG)定量测定作为检测指标,Western blot检测蛋白水平的表达情况.结果HGI孵育减少脂肪细胞葡萄糖摄取,促进细胞内TG积聚,同时伴有ATGL及HSL的蛋白表达下调.Neu-p11干预逆转了HGI对脂肪细胞的作用效应,而MT2竞争性拮抗剂luzindole却拮抗了Neu-p11的上述效应.结论Neu-p11以MT2受体依赖性方式抑制IR脂肪细胞TG沉积,可能与其上调ATGL、HSL蛋白的表达,促进TG水解相关.
作者机构：
[Huang Ying; Li Guoqing; Xiao Zhefeng; Li Maoyu; Peng Fang; Hu Rong; Shao Meiying; Chen Xiaojuan; Li Yuanyuan; Zhan Xianquan; Chen Zhuchu] State Local Joint Engineering Laboratory for Anticancer Drugs,Hunan Engineering Laboratory for Structural BiologyDrug Design,Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Xiangya Hospital,Central South University,Changsha,P.R.China,410008;[Li Guoqing] Department of Biology,School of Pharmacy and Life Science,University of South China,Hengyang,Hunan,P.R.China,410008;[Li Yuanyuan] Medical College,Guangxi University of Science and Technology,Liuzhou,Guangxi,P.R.China,545005
作者机构：
[Cao, Jiangang; Chen, Linxi; Lv, Deguan; Lu, Qixuan] Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, China
通讯机构：
[Linxi Chen *] I;Institute of Pharmacy and Pharmacology , University of South China , Hengyang 421001 , China
关键词：
Unanticipated;miRNA;apelin
摘要：
Recently, a paper by Kim et al [1] in Nature Medicine magazine in January, 2013 showed that apelin (also known as APLN) inhibits fibroblast growth factor 2 (FGF2) and FGF receptor 1 (FGFR1) expression to ameliorate pulmonary hypertension by regulating the expression of miR-424 and miR-503. This study revealed the molecular mechanism of apelin in inhibiting the process of pulmonary arterial hypertension (PAH) and discovered the role of apelin in regulating miRNA generation for the first time. miRNA functions in the transcriptional regulation of gene expression to control cellular processes. miRNA is a key regulatory factor of protein expression, but the generation and regulation mechanism of miRNA is still unclear. These novel findings bring us inspiration for further research, especially on the mechanism of miRNA generation. Experiments on revealing endogenous active substance, which regulates the generation of miRNAs or revealing miRNAs that regulate the expression of apelin, may bring more breakthroughs in the future.
摘要：
Despite the established efficacy of statin therapy, the risk of cardiovascular events remains high in many patients. We examined high-density lipoprotein (HDL) subclass distribution profiles among statin-treated coronary heart disease (CHD) patients undergoing percutaneous coronary intervention (PCI). Plasma HDL subclasses were measured in 85 patients with established CHD and quantified by two-dimensional gel electrophoresis and immunoblotting. In CHD patients with statin treatment, the mean value of total cholesterol (TC) reached the desirable level and the triacylglycerol level (TAG) was borderline high. Moreover, low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apolipoproteinA-I, and apolipoproteinB-100 levels in these patients resembled those in normolipidemic healthy subjects. The HDL subclass did not show a normal distribution and was characterized by the lower large-sized HDL2b contents and higher contents of small-sized preβ1-HDL in CHD patients, compared to those in normolipidemic control subjects. Multiple stepwise regression analysis revealed that the severity of coronary stenosis, determined by the Gensini Score, was significantly and independently predicted by HDL2b and HDL3b. Statin therapy was effective in modifying plasma lipids levels, but not adequate as a monotherapy to normalize the HDL subclass distribution phenotype of patients with CHD undergoing PCI. The HDL subclass distribution may aid in risk stratification, especially in patients with CHD and therapeutic LDL-C and HDL-C levels.
关键词：
genotoxicity;heavy metal (HM);micronucleus (MN);polycyclic aromatic hydrocarbon (PAH);soil
摘要：
During the past few decades, urban and suburban developments have grown at unprecedented rates and extents with unknown consequences for ecosystem function. The problem of soil pollution as a result of the accelerating development of Guangzhou in China is becoming great concerns. In the present study, gas chromatograph coupled mass spectrometry (GC-MS), inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma atomic emission spectrometry (ICP-AES) were employed to determine the 16 US Environmental Protection Agency (EPA) priority polycyclic aromatic hydrocarbons (PAHs) and the heavy metals (As, Cr, Cu, Pb, Cd, Hg, and Se) of soils collected from suburban areas of Guangzhou. The genotoxicity of these soils was screened with micronucleus (MN) assay in Vicia faba root cells. The concentrations of the pollutants in the soils were (dried weight): ΣPAHs (230.6–1263 ng·g−1), As (2282.6–36064 μg·kg−1), Cr (7109–64699 μg·kg−1), Cu (7047–56388 μg·kg−1), Pb (9675.9–93739 μg·kg−1), Cd (68.5–847.3 μg·kg−1), Hg (85.4–549.2 μg·kg−1), and Se (219.2–968 μg·kg−1), which fell in the moderately polluted range. However, six out of nine soil-exposed groups had a significant increases of MN frequencies observed in the V. faba root cells compared with the negative group (P < 0.05, P < 0.01), indicating that they had potential genotoxic risks. Bringing together the chemical analyses with the biological effects observed in this study, the genotoxic response could at a certain degree be explained by both the soil PAHs and heavy metals. Our results suggested that apart from chemical analysis, bioassays like the MN assay of V. faba root cells should also be included in a battery of tests to assess the eco-environmental risks of urban and/or urbanization in the developing areas on the soils.
期刊：
BioTechnology: An Indian Journal,2013年8(4):543-550 ISSN：0974-7435
通讯作者：
Liao, D.-F.(dfliao66@aliyun.com)
作者机构：
[Tuo, Qin-Hui; Liao, Duan-Fang; Gong, Yong-Zhen; Sun, Shao-Wei] Hunan University of Chinese Medicine, Hanpu Science and Education District, 1# Xiangzui Road, Changsha 410208, Hunan, China;[Ke-Tang, Chao] Institute of Cardiovascular Disease, Life Science Research Center, University of South China, 28# Changsheng Western Road, Hengyang 421001, Hunan, China;[Tuo, Qin-Hui; Ke-Tang, Chao; Liao, Duan-Fang; Gong, Yong-Zhen; Zhang, Xiao-Ying; Sun, Shao-Wei] Division of biochemistry, School of pharmacy and Life Science, University of South China, 28# Changsheng West Road, Hengyang 421001, Hunan, China;[Zhang, Xiao-Ying] Division of Pharmacoproteomics, School of pharmacy and Life Science, University of South China, 28# Changsheng Western Road, Hengyang 421001, Hunan, China
通讯机构：
[Liao, D.-F.] H;Hunan University of Chinese Medicine, 1# Xiangzui Road, Changsha 410208, Hunan, China
摘要：
Phytochemical investigation of the fresh tubers of Ophiopogon japonicus led to the isolation of two new furostanol saponins (1 and 2) together with two known steroidal saponins (3 and 4). Comprehensive spectroscopic analysis allowed the chemical structures of two new compounds to be elucidated as (25R)-26-O-[beta-d-glucopyranosyl-(1 --> 2)-beta-d-glucopyranosyl]-5-ene-furost-1beta,3beta,22alpha,26-tetraol-3-O-alpha-l -rhamnopyranosyl-(1 --> 2)-[beta-d-xylopyranosyl-(1 --> 4)]-beta-d-glucopyranoside (1, ophiopogonin P) and (25R)-26-O-[beta-d-glucopyranosyl-(1 --> 6)-beta-d-glucopyranosyl]-5-ene-furost-1beta,3beta,22alpha,26-tetraol-3-O-alpha-l -rhamnopyranosyl-(1 --> 2)-[beta-d-xylopyranosyl-(1 --> 4)]-beta-d-glucopyranoside (2, ophiopogonin Q). Furostanol saponins with the disaccharide chain linked at C-26 hydroxy group of the aglycone have been rarely reported from natural sources.
摘要：
MicroRNAs are a group of endogenous, small non-coding RNA molecules that can induce translation repression of target genes within metazoan cells by specific base pairing with the mRNA of target genes. Recently, microRNA-33 has been discovered as a key regulator in the initiation and progression of atherosclerosis. This review highlights the impact of microRNA-33-mediated regulation in the major cardiometabolic risk factors of atherosclerosis including lipid metabolism (HDL biogenesis and cholesterol homeostasis, fatty acid, phospholipid and triglyceride, bile acids metabolism), inflammatory response, insulin signaling and glucose/energy homeostasis, cell cycle progression and proliferation, and myeloid cell differentiation. Understanding the etiology and pathophysiology of microRNA-33 in atherosclerosis may provide basic knowledge for the development of novel therapeutic targets for ameliorating atherosclerosis and cardiovascular disease.
