CpG adjuvant enhances the mucosal immunogenicity and efficacy of a Treponema pallidum DNA vaccine in rabbits
作者：
Zhao, Feijun;Liu, Shuangquan;Zhang, Xiaohong;Yu, Jian;Zeng, Tiebing;...
期刊：
Human Vaccines & Immunotherapeutics ,2013年9(4):753-760 ISSN：2164-5515
通讯作者：
Wu, Yimou
作者机构：
[Wu, Yimou; Zhao, Feijun; Zeng, Tiebing] Univ South China, Pathogen Biol Inst, Hengyang City, Hunan, Peoples R China.;Centers Dis Control Hunan Prov, Changsha, Hunan, Peoples R China.;[Liu, Shuangquan] Cent S Univ, Dept Anat & Neurobiol, Xiangya Sch Med, Changsha, Hunan, Peoples R China.;[Liu, Shuangquan; Cao, Xunyu] Univ South China, Affiliated Hosp 1, Hengyang, Hunan, Peoples R China.;[Zhang, Xiaohong] Univ South China, Dept Histol & Embryol, Sch Med, Hengyang, Hunan, Peoples R China.
通讯机构：
[Wu, Yimou] U;Univ South China, Pathogen Biol Inst, Hengyang City, Hunan, Peoples R China.
关键词：
DNA vaccine;CpG ODN;membrane protein;Treponema pallidum;Tp Gpd;mucosal adjuvant;mucosal immune
摘要：
Objectives: The protective response against Treponema pallidum (Tp) infection of a DNA vaccine enhanced by an adjuvant CpG ODN was investigated. Results: The mucosal adjuvant CpG ODN enhanced the production of higher levels of anti-TpGpd antibodies induced by pcD/Gpd-IL-2 in rabbits. It also resulted in higher levels of secretion of IL-2 and IFN-α, and facilitated T cell proliferation and differentiation (p < 0.05). No significant difference about testing index above-mentioned was found in the intranasal immunization group of pcD/Gpd-IL-2 vaccine adjuvanted by CpG ODN when compared with the immunization by pcD/ Gpd-IL-2 vaccine intramuscular injection alone (p > 0.05). Furthermore, CpG ODN stimulated the production of mucosaspecific anti-sIgA antibodies and resulted in the lowest Tp-positive rate (6.7%) for Tp-infection of skin lesions and the lowest rates (8.3%) of ulceration lesions, thus achieving better protective effects. Methods: New Zealand rabbits were immunized with the eukaryotic vector encoding recombinant pcD/Gpd-IL-2 using intramuscular multi-injection or together with mucosal enhancement via a nasal route. The effect of the mucosal adjuvant CpG ODN was examined. Conclusions: The CpG ODN adjuvant significantly enhances the humoral and cellular immune effects of the immunization by pcD/Gpd-IL-2 with mucosal enhancement via nasal route. It also stimulates strong mucosal immune effects, thus initiating more efficient immune-protective effects. © 2013 Landes Bioscience.
语种：
英文
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弱氧化低密度脂蛋白对小鼠肠系膜动脉内皮依赖性舒张功能的影响及机制
作者：
陈根;秦旭平;林杰;王俊杰;周楠;...
期刊：
药学学报 ,2013年48(11):1657-1664 ISSN：0513-4870
通讯作者：
Li, J.
作者机构：
[陈根; 林杰; 秦旭平] Institute of Drug and Pharmacology, University of South China, Hengyang 421001, China;[李洁] The First People's Hospital of Chenzhou, Chenzhou 423000, China;[王俊杰] Xiang Nan University, Chenzhou 423000, China;[周楠] Medical College, Xi 'an Jiaotong University, Xi 'an 710061, China
通讯机构：
Institute of Drug and Pharmacology, University of South China, China
关键词：
内皮依赖性舒张;一氧化氮;内皮源性超级化因子;肠系膜动脉
摘要：
探讨mmLDL对小鼠肠系膜动脉内皮依赖性舒张功能的影响及机制.采用离体微血管张力描记技术,透射电镜观察血管内皮超微结构.结果显示,mmLDL致小鼠肠系膜动脉内皮细胞明显水肿、内弹力膜断裂；与生理盐水组Rmax (63±5)％和pIC50 6.42± 0.09比较,显著抑制EDHF介导的舒张[Rmax(31±3)％和pIC50 5.67±0.07,P＜0.001,P＜0.001];与生理盐水组[Rmax(45±4)％和pICs0 5.93±0.08]比较,显著抑制NO介导的舒张[Rmax(32±4)％和pIC50 5.43±0.11,P＜0.05,P＜0.01]；对PGI2介导的舒张功能无明显影响.综上所述,mmLDL损伤内皮细胞超微结构;显著降低内皮依赖性舒张功能与NO-和EDHF途径受损有关.
语种：
中文
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微卫星标记预警实验红鲫对苯酚遗传毒性响应的研究
作者：
朱晓娟;董先辉;练高建;吴端生
期刊：
实验动物科学 ,2013年30(2):23-31+41 ISSN：1006-6179
作者机构：
南华大学药学与生物科学学院实验动物学部,衡阳,421001;[练高建; 吴端生; 董先辉; 朱晓娟] 南华大学
关键词：
微卫星标记;苯酚;实验红鲫C1HD系
摘要：
目的探讨微卫星标记预警实验红鲫对苯酚遗传毒性的响应。方法选择5个微卫星标记,观察4个苯酚染毒组经8 d染毒实验,苯酚对实验红鲫C1HD系微卫星多态性的影响。结果苯酚在实验红鲫C1HD系体内的富集提高了基因杂合度（H）、基因多态性信息含量（PIC）等群体遗传学指数。在1/8 LC50苯酚浓度组染毒第6天,基因杂合度（H=0.15±0.19）和基因多态性信息含量（PIC=0.42）均达到峰值。结论体内富集一定浓度的苯酚可致实验红鲫C1HD系微卫星多态性改变。微卫星基因杂合度（H）和多态性信息含量（PIC）随苯酚染毒时间的延长和富集量的增加而升高。
语种：
中文
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CURCUMIN TRINICOTINATE REGULATE THE PROLIFERATION OF VSMCS VIA CAVEOLIN-1/ERK1/2 SIGNALING PATHWAY
作者：
Yang, W.;Li, T. P.;Tuo, Q. H.;Zheng, X.;Guo, Z. F.;...
期刊：
CARDIOLOGY ,2013年125(SUPPL-2):465-465 ISSN：0008-6312
作者机构：
[Yang, W.; Zheng, X.; Liao, D. F.; Li, T. P.] Univ South China, Dept Pharm & Pharmacol, Hengyang, Hunan, Peoples R China.;[Guo, Z. F.; Tuo, Q. H.] Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China.
语种：
英文
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“五位一体”教学法在生物技术教学中的作用
作者：
贺庆芝;秦志峰;易岚;李国庆;殷杰
期刊：
中国校外教育 ,2013年(z1):231-231 ISSN：1004-8502
作者机构：
南华大学药学与生命科学院生物科学系;[殷杰] 殷杰;[李国庆] 李国庆;[秦志峰] 秦志峰;[易岚] 易岚
关键词：
五位一体;生物技术教学;学习主动性
摘要：
“五位一体”教学法是指将专业教育、学业教育、实践教育、创业教育、综合教育融合为一体的教学方法。在生物技术的教学中运用五位一体教学法具有重要的作用，本文将对五位一体教学法在生物技术教学中的作用进行探讨。
语种：
中文
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Deuterium-depleted water (DDW) inhibits the proliferation and migration of nasopharyngeal carcinoma cells in vitro
作者：
Wang, Hongqiang;Zhu, Baohua;He, Zhiwei;Fu, Hui;Dai, Zhong;...
期刊：
Biomedicine & Pharmacotherapy ,2013年67(6):489-496 ISSN：0753-3322
通讯作者：
Fang, Weiyi
作者机构：
[He, Zhiwei; Wang, Hongqiang; Li, Binbin; Huang, Guoliang; Yang, Huiling; Zhu, Baohua; Dai, Zhong; Qin, Dongyun; Zhang, Xiaoyan; Fu, Hui] Guangdong Med Coll, Sino Amer Canc Res Inst, Dongguan 523808, Peoples R China.;[Wang, Hongqiang; Yang, Huiling] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Fang, Weiyi] Southern Med Univ, Canc Res Inst, Guangzhou 510515, Guangdong, Peoples R China.;[Tian, Lu] Guangdong Pharmaceut Univ, Inst Mat Med, Guangzhou 510006, Guangdong, Peoples R China.
通讯机构：
[Fang, Weiyi] S;Southern Med Univ, Canc Res Inst, Guangzhou 510515, Guangdong, Peoples R China.
关键词：
Cell proliferation;Deuterium-depleted water (DDW);NADPH:quinone oxidoreductase-1 (NQO1);Nasopharyngeal carcinoma cell (NPC);Preosteoblast cell
摘要：
Recent studies have demonstrated that natural water that has 65% of the deuterium concentration depleted, can exhibit anti-tumor properties. However, the anti-tumor effects of DDW on various nasopharyngeal carcinoma (NPC) cells have not previously been reported. In the present study, NPC cell lines and normal preosteoblast MC3T3-E1 cells were grown in RPMI1640 media containing different deuterium concentrations (50-150. ppm). The effects of DDW on the proliferation and migration of NPC and MC3T3-E1 cells were investigated using the MTT, plate colony formation, and Transwell assays, as well as Boyden chamber arrays, flow cytometry (FCM), western blot and immunofluorescence. We found that DDW was an effective inhibitor of NPC cell proliferation, plated colony formation, migration and invasion. In contrast, the growth of normal preosteoblast MC3T3-E1 cells was promoted when they were cultured in the presence of DDW. Cell cycle analysis revealed that DDW caused cell cycle arrest in the G1/S transition, reduced the number of cells in the S phase and significantly increased the population of cells in the G1 phase in NPC cells. Western blot analysis revealed that treatment with DDW significantly increased the expression of NADPH:quinone oxidoreductase-1 (NQO1), while immunofluorescence assay analysis revealed that treatment with DDW decreased the expression of PCNA and matrix metalloproteinase 9 (MMP9) in NPC cells. These results demonstrated that DDW is a novel, non-toxic adjuvant therapeutic agent that suppresses NPC cell proliferation, migration, and invasion by inducing the expression of NQO1 and causing cell cycle arrest, as well as decreasing PCNA and MMP9 expression. © 2013 Elsevier Masson SAS.
语种：
英文
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Characterization and fabrication of multi-layer films based on polysaccharide and cellulose
作者：
Yu, Cui-Yun;Liu, Ge-Sha;Li, Na-Mei;Wang, Yan-Mei;He, Dong-Xiu;...
期刊：
Journal of Controlled Release ,2013年172(1):E126-E127 ISSN：0168-3659
作者机构：
[He, Dong-Xiu; Wang, Yan-Mei; Lei, Xiao-Yong; Li, Na-Mei; Liu, Ge-Sha; Yu, Cui-Yun] Univ South China, Dept Pharm, Hengyang 421001, Peoples R China.
会议名称：
2nd Symposium on Innovative Polymers for Controlled Delivery(第二届新型高分子材料与控制释放国际会议(SIPCD2012))
会议时间：
2012-9-11
会议地点：
苏州
会议主办单位：
中科院;苏州大学
会议论文集名称：
2nd Symposium on Innovative Polymers for Controlled Delivery(第二届新型高分子材料与控制释放国际会议(SIPCD2012))论文集
关键词：
multi-layer films;polysaccharide;cellulose;layer-by-layer assembly
摘要：
Aims & ScopeThe Journal of Inclusion Phenomena and Macrocyclic Chemistry is the premier interdisciplinary publication reporting on original research into all aspects of host-guest systems. Examples of specific areas of interest are: the preparation and characterization of new hosts and new host-guest systems, especially those involving macrocyclic ligands; crystallographic, spectroscopic, thermodynamic and theoretical studies; applications in chromatography and inclusion polymerization; enzyme modelling; molecular recognition and catalysis by inclusion compounds; intercalates in biological and non-biological systems, cyclodextrin complexes and their applications in the agriculture, flavoring, food and pharmaceutical industries; synthesis, characterization and applications of zeolites. The journal publishes primarily reports of original research and preliminary communications, provided the latter represent a significant advance in the understanding of inclusion science. Critical reviews dealing with recent advances in the field are a periodic feature of the journal.Coverage in the Journals&commat;Ovid database begins with the January 1999 issue.
语种：
英文
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Assessment of heavy metal contamination and bioaccumulation in soybean plants from mining and smelting areas of southern Hunan Province, China
作者：
Zhou, Hang;Zeng, Min;Zhou, Xin;Liao, Bo-Han* ;Liu, Jun;...
期刊：
Environmental Toxicology and Chemistry ,2013年32(12):2719-2727 ISSN：0730-7268
通讯作者：
Liao, Bo-Han
作者机构：
[Zhou, Hang] Hunan Agr Univ, Coll Biosci & Technol, Changsha, Hunan, Peoples R China.;[Zhong, Qian-Yun; Liao, Bo-Han; Zeng, Hui; Zeng, Min; Zhou, Xin; Lei, Ming; Zhou, Hang] Cent South Univ Forestry & Technol, Inst Environm Sci & Engn, Changsha, Hunan, Peoples R China.;[Liu, Jun] Univ South China, Coll Pharm & Life Sci, Hengyang, Peoples R China.
通讯机构：
[Liao, Bo-Han] C;Cent South Univ Forestry & Technol, Inst Environm Sci & Engn, Changsha, Hunan, Peoples R China.
关键词：
Assessment;Geoaccumulation index;Heavy metal accumulation;Soil contamination;Soybean (Glycine max)
摘要：
Soybean is one of most important dicotyledonous food crops and is widely planted in Hunan Province, China. However, mining activity causes contamination of the soil in which soybean grows. To assess the impact of mining-induced soil contamination on soybean plants, a geoaccumulation index (I<inf>geo</inf>) was used to evaluate 20 soil samples from the mining and smelting areas of southern Hunan Province. The results indicated that Zn ranged from uncontaminated to a moderately contaminated level (I<inf>geo</inf><1), Pb was at a strongly contaminated level (I<inf>geo</inf>>3), and Cd was at an extremely contaminated level (I<inf>geo</inf>>5) across the whole study area. All of the studied soybean plants were affected by heavy metal Pb and Cd contamination, and the mean concentrations in seeds were 13.9mg/kg and 2.95mg/kg, respectively. The estimated bioconcentration factor and translocation factor showed that the soybean roots had a strong Cd bioconcentration capability and the stems had a strong translocation capability in terms of Pb, Cd, and Zn, with preferential transference of metals to the soybean leaves. The bioavailable fraction in the soil was characterized by the exchangeable fraction of heavy metals. In the present study, the bioavailable fractions of Pb, Cd, and Zn were significantly positively correlated with the concentration of these metals in soybean tissues (roots, stems, leaves, husks, and seeds). ©2013 SETAC.
语种：
英文
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Efficient discrimination of three hotspot mutations associated with aminoglycoside antibiotics induced deafness in the mitochondrial 12S rRNA by on/off switch
作者：
Xuan, Gui-ping;Guo, Zi-fen;Chen, Fang-fang;Dong, Wei-lei
期刊：
中国药理学报 ,2013年34(SUPPL-S):157-157 ISSN：1671-4083
作者机构：
[Guo, Zi-fen; Chen, Fang-fang; Xuan, Gui-ping] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Dong, Wei-lei] Univ South China, Affiliated Hosp 1, Hengyang 421001, Peoples R China.
会议名称：
The 12th Meeting of the Asia Pacific Federation of Pharmacologists(第十二届亚洲太平洋地区药理学家联盟会议暨中国药理学会第十二次全国学术会议)
会议时间：
2013-7-9
会议地点：
上海
会议主办单位：
中国药理学会
会议论文集名称：
The 12th Meeting of the Asia Pacific Federation of Pharmacologists(第十二届亚洲太平洋地区药理学家联盟会议暨中国药理学会第十二次全国学术会议)论文集
关键词：
Exo(+) polymerase;phosphorothioate modification;mitochondrial 12S rRNA;point mutation;real-time PCR
摘要：
<正>Aim:The 961delT,C1494T and A1555G mutations in the mitochondrial 12S rRNA are the hotspot mutations associated with aminoglycoside antibiotics induced deafness(AAID).The objective of this study was
语种：
英文
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Piromelatine decreases triglyceride accumulation in insulin resistant 3T3-L1 adipocytes: Role of ATGL and HSL
作者：
Wang, Ping-Ping;She, Mei-Hua;He, Ping -Ping;Chen, Wu-Jun;Laudon, Moshe;...
期刊：
Biochimie ,2013年95(8):1650-1654 ISSN：0300-9084
通讯作者：
Yin, Wei-Dong
作者机构：
[Xu, Xuan-Xuan; He, Ping -Ping; Yin, Wei-Dong; Chen, Wu-Jun; Wang, Ping-Ping] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Yin, Wei-Dong; She, Mei-Hua] Univ South China, Dept Biochem & Mol Biol, Sch Life Sci & Technol, Hengyang 421001, Hunan, Peoples R China.;[Chen, Wu-Jun] Univ South China, Inst Pharm, Hengyang 421001, Hunan, Peoples R China.;[Chen, Wu-Jun] Univ South China, Inst Pharmacol, Hengyang 421001, Hunan, Peoples R China.;[Laudon, Moshe] Neurim Pharmaceut Ltd, Drug Discovery, Tel Aviv, Israel.
通讯机构：
[Yin, Wei-Dong] U;Univ South China, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词：
3T3-L1 adipocyte;ATGL HSL;Ca2+;Insulin resistance;Piromelatine;Triglyceride
摘要：
Piromelatine, a novel investigational multimodal sleep medicine, is developed for the treatment of patients with primary and co-morbid insomnia. Piromelatine has been shown to inhibit weight gain and improve insulin sensitivity in high-fat/high-sucrose-fed (HFHS) rats. Considering that piromelatine has also been implicated in lowering of triglyceride levels in HFHS rats, this work elucidated whether this effect involves in the regulation of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) in triglyceride (TG) metabolism. In this study, we investigated the effects of piromelatine and MT2 receptors inhibition on TG content, insulin-stimulated glucose uptake, and the expressions of ATGL and HSL in 3T3-L1 adipocytes preincubated in high glucose and high insulin (HGI) conditions. Our results showed that culturing 3T3-L1 adipocytes under HGI conditions increased triglyceride accumulation with concomitant decrease of ATGL and HSL expression, inducing insulin resistance in 3T3-L1 adipocytes. We also found that triglyceride accumulation was significantly inhibited and the levels of ATGL/HSL increased after melatonin or piromelatine treatment. The effects of melatonin/piromelatine (10 nM) were counteracted by pretreatment with the relatively selective MT2 receptor antagonist luzindole (100 nM). In this study, our data demonstrate that piromelatine reverses high glucose and high insulin-induced triglyceride accumulation in 3T3-L1 adipocytes, possibly through up-regulating of ATGL and HSL expression via a melatonin-dependent manner. © 2013 Elsevier Masson SAS. All rights reserved.
语种：
英文
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胶体金标记抗甲基苯丙胺复合物的合成
作者：
王五洲;何芳丽;乔新惠
期刊：
微量元素与健康研究 ,2013年30(5):50-51 ISSN：1005-5320
作者机构：
南华大学生物化学与分子生物学实验中心 湖南衡阳421001;南华大学生化与分子生物学教研室 湖南衡阳421001
关键词：
胶体金;抗甲基苯丙胺单克隆抗体;合成
摘要：
目的：合成高质量的胶体金标记抗甲基苯丙胺复合物,以应用于能检测吸毒人员尿液中是否含有甲基苯丙胺的胶体金试纸条。方法：用柠檬酸三钠还原法制备胶体金,用抗甲基苯丙胺单克隆抗体标记胶体金。结果：成功制备了直径约20 nm颗粒均一的胶体金,进一步制备了高质量的胶体金标记抗甲基苯丙胺复合物。结论：制备的胶体金标记抗甲基苯丙胺复合物活性较好,能用于检测甲基苯丙胺的胶体金试纸条。
语种：
中文
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高龄化社会退休人力的社会参与分析与活化策略建构:以台湾社会的经验为例
作者：
魏书娥
作者机构：
[魏书娥] 南华大学生死学系
会议名称：
第三届"海峡两岸农村社会保险理论与实践"学术研讨会
会议时间：
2013-01-01
会议地点：
中国江苏南京
会议论文集名称：
海峡两岸农村社会保险理论与实践研究论文集
关键词：
高龄化社会;活化老化;社会参与
摘要：
世界卫生组织提出"活化老化"是迈入晚期工业资本主义经济阶段的高龄化社会之人,化解老年退休危机的核心观点。在此观点之下,老年退休者重新建立与社会连结的落实方式则必须仰赖劳动参与以及社会参与,劳动参与方式牵涉高龄社会的劳动市场结构变动所冲击的老年经济安全,而社会参与方式牵涉资本主义社会的社会资本类型所影响老年生活质量,两者之间的选取对退休者而言,基于其时间、体力和意愿的限制,既相互排挤,又相辅相成,尚
语种：
中文
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Effect of different wavelength ultraviolet radiation on photoreactivation in Vibrio parahaemolyticus
作者：
苏泽红;何淑雅;李俐娟;练高建;高桥章
期刊：
现代食品科技 ,2013年29(12):2889-2893 ISSN：1673-9078
通讯作者：
Lian, G.-J.
作者机构：
[李俐娟; 何淑雅; 苏泽红] Department of Biotechnology, School of Pharmacology and Life Sciences and Technology, University of South China, Hengyang 421001, China;[Takahashi, Akira] Department of Preventive and Environment Nutrition, Tokushima University, Tokushima, 770-8501, Japan;[练高建] Department of Experimental Zoology, University of South China, Hengyang 421001, China
通讯机构：
Department of Experimental Zoology, University of South China, China
关键词：
紫外照射;副溶血性弧菌;光修复反应;发光二极管
摘要：
低压水银管是常见的灭菌工具,其一般采用的是波长为254 nm的UVC作为灭菌光源.而UVA(365 nm波长)配合发光二极管(light-emitting diodes,LED)也有明显的杀菌效果.本研究从灭菌能力,照射后细菌的光修复能力和再生长能力以及能耗等方面入手,比较短波长的UVC和长波长的UVA/LED对副溶血性弧菌的灭菌效果.结果显示,UVA/LED比UVC有更强的杀菌能力,且在受到UVA/LED照射后,副溶血性弧菌的再生长明显慢于单纯UVC处理后的细菌;对副溶血性弧菌的光修复酶基因表达水平进行检测发现,UVA/LED照射后副溶血性孤菌三种光修复酶基因的表达水平都无激活现象,而UVC处理后的细菌三种基因表达水平明显升高.我们认为UVA/LED可能抑制副溶血性弧菌的光修复现象.但在具备以上优势的同时,UVA/LED的能耗大大高于UVC,因此该技术还有待进一步改进.
语种：
中文
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MicroRNA-98 sensitizes cisplatin-resistant human lung adenocarcinoma cells by up-regulation of HMGA2
作者：
Xiang, Qiong;Tang, Huifang;Yu, Jia;Yin, Jie;Yang, Xiaoyan;...
期刊：
PHARMAZIE ,2013年68(4):274-281 ISSN：0031-7144
通讯作者：
Lei, Xiaoyong
作者机构：
[Tang, Huifang] Univ South China, Affilitated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;[Lei, Xiaoyong] Univ South China, Inst Pharm & Pharmacol, 28 Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Lei, Xiaoyong] U;Univ South China, Inst Pharm & Pharmacol, 28 Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
摘要：
This study was done to explore the role of microRNA-98 (miR-98) in cisplatin sensitization in human lung adenocarcinoma cell line. Differential expressions of miRNAs were analysed between cisplatin-resistant human lung adenocarcinoma cell line A549/DDP and its parental cell A549 by miRNAs microarray, of which 14 miRNAs were showed to be significantly (> 2-fold) up-regulated and 8 miRNAs had marked down-regulation (< 0.5-fold) in A549/DDP cells compared with in A549 cells. MiR-98, a member in the let-7 family, acts as a negative regulator in the expression of HMGA2 (high mobility group A2) oncogene, and it has been shown to have a nearly 3-fold decrease in A549/DDP cells. We found that elevated expression of miR-98 led to a higher sensitivity of A549/DDP cells to cisplatin, and the protein level of HMGA2, was clearly up-regulated in both A549/DDP and A549 cells by miR-98. Moreover, both Bcl-XL and Bcl-2, were down-regulated in the Pre-miR-98(TM) transfectants cells. We for the first time demonstrated that the expression of miR-98 increases cells spontaneous apoptosis and sensitizes cells to cisplatin at least in part via HMGA2 up-regulation. Our findings provided insight into some specific miRNAs in lung cancer as potential therapeutic targets.
语种：
英文
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生物科学专业基因工程教学改革初探
作者：
苏泽红;练高建;何淑雅;尹卫东;李斌元
期刊：
广东化工 ,2013年40(18):157+159 ISSN：1007-1865
作者机构：
南华大学药学与生物科学学院,湖南衡阳,421001;南华大学实验动物学部,湖南衡阳,421001
关键词：
基因工程;生物科学;网络教学;集中实验教学
摘要：
《基因工程》是我校生物科学专业的主干课程之一.该课程是一门具有很强实践性的技术学科,本课程教学质量的好坏将直接关系到学生的专业素质、动手能力以及创新能力的培养.本教研室从教学内容、教学方法和手段、构建网络教学平台,实验教学和考核方式等五个方面入手,对《基因工程》传统的教学模式进行了一系列的改革,实践证明取得了一定的成效.
语种：
中文
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Caspase-3 is Involved in IFN-γ- and TNF-α-Mediated MIN6 Cells Apoptosis via NF-κB/Bcl-2 Pathway
作者：
Cao, Zhao-hui;Yin, Wei-dong;Zheng, Quan-you;Feng, Shao-long;Xu, Gui-lian* ;...
期刊：
Cell Biochemistry and Biophysics ,2013年67(3):1239-1248 ISSN：1085-9195
通讯作者：
Xu, Gui-lian
作者机构：
[Yin, Wei-dong; Feng, Shao-long; Cao, Zhao-hui] Univ South China, Sch Pharm & Life Sci, Hengyang 421001, Peoples R China.;[Xu, Gui-lian; Cao, Zhao-hui] Third Mil Med Univ, Dept Immunol, Chongqing 400038, Peoples R China.;[Zheng, Quan-you; Zhang, Ke-qin; Cao, Zhao-hui] Third Mil Med Univ, Inst Surg Res, Daping Hosp, Dept Urol, Chongqing 400042, Peoples R China.
通讯机构：
[Xu, Gui-lian] T;Third Mil Med Univ, Dept Immunol, Chongqing 400038, Peoples R China.
关键词：
MIN6 cells;Caspase-3;NF-kappa B;Bcl-2;Apoptosis
摘要：
TNF-α and IFN-γ are the major pro-inflammatory cytokines in the β-cell destruction. However, the underlying mechanism remains unclear. The present study used a murine insulinoma cell line MIN6 for further investigation of the effect of Caspase-3 on the cytokines-induced pancreatic β-cell apoptosis and analyzed the mechanisms involved in the activation of Caspase-3. It was showed that the combination of IFN-γ and TNF-α significantly reduced the viability of MIN6 cells and the observed cells growth inhibition was due to cell apoptosis as judged by the morphological changes under a confocal laser scanning microscopy and FACS assay of Annexin-V/7-AAD double staining. Accompanying with NF-κB activation and Bcl-2 downregulation, both the cleaved Caspase-3 and PARP, a known substrate of Caspase-3 in vivo, were observed at 24 and 12 h, respectively, after cells exposure to IFN-γ and TNF-α treatment. Pretreatment of Caspase-3 inhibitors remarkably attenuated IFN-γ- and TNF-α-induced cells apoptosis. Inhibition of NF-κB activation led to the increase in Bcl-2 expression, a significant attenuation in Caspase-3 activity, and an obvious amelioration in cells viability in IFN-γ- and TNF-α-treated MIN6 cells. Taken together, our results indicate that Caspase-3 is critical for the induction of MIN6 cells apoptosis and it's activation is further confirmed to be related to the NF-κB-mediated Bcl-2 downregulation, which may be the underlying mechanism of IFN-γ- and TNF-α-mediated MIN6 cells apoptosis. © 2013 Springer Science+Business Media New York.
语种：
英文
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＂五位一体＂应用型创新人才培养模式的构建
作者：
贺庆芝;曾怀才;彭翠英
期刊：
新西部：下旬·理论 ,2013年(7):59+53 ISSN：1009-8607
作者机构：
南华大学药学与生命科学院 湖南衡阳421001
关键词：
五位一体;应用型;创新人才;培养模式;构建
摘要：
要培养“品格、素质、知识、技能、思想”相结合的“五位一体”的应用型创新人才，就必须提高思想认识，加强师资队伍建设，构建囊括教学、提升能力和思想培养等活动相结合的全新教育模式，矾究建立完整的创新教学体系。
语种：
中文
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壳聚糖纳米包裹的IL-2与TpGpd核酸疫苗联合免疫后对Tp感染兔皮肤前后的免疫效应评估
作者：
赵飞骏;张晓红;刘双全;曾铁兵;余坚;...
期刊：
中国科学：生命科学 ,2013年43(2):151-158 ISSN：1674-7232
作者机构：
[赵飞骏; 曾铁兵; 张跃军; 吴移谋] 南华大学病原生物学研究所;[张晓红] 南华大学组织学与胚胎学教研室;[刘双全] 南华大学附属第一医院检验科;[余坚] 南华大学实验动物学部;[顾伟鸣] 上海市皮肤病性病医院检验科
关键词：
梅毒螺旋体;膜蛋白;核酸疫苗
摘要：
梅毒是一种由梅毒螺旋体(Treponema pallidum,Tp)引起的临床表现复杂的性传播疾病,近年来世界各国包括中国的梅毒发病率呈逐年上升趋势,研制有效疫苗预防该种疾病已成为各国关注的公共卫生问题.DNA疫苗具有免疫效果好且持久、无毒力回复等特点成为近年来研究热点.对于Tp核酸疫苗研究,国内外除了本课题组构建TpDNA疫苗免疫新西兰兔进行免疫活性的初步研究外,尚未见Tp核酸疫苗的相关研究.本研究旨在探讨评价白细胞介素-2(interleukin-2,IL-2)与Tp膜蛋白GpdDNA疫苗用壳聚糖(chitosan,CS)纳米颗粒包裹联合免疫新西兰兔后对Tp皮肤感染后的保护效应.将真核表达重组体pcDNA3.1(+)/IL-2和pcDNA3.1(+)/TpGpd采用壳聚糖纳米颗粒包裹或未包裹方式处理后分别或联合免疫新西兰兔,每隔2周加强1次共免疫3次,并于初次免疫后第10周各实验组兔皮下接种Tp标准株进行背部皮肤感染实验,观察记录感染早期各实验组皮损的变化情况,在免疫及感染期间不同时间点ELISA检测免疫兔特异性抗体产生水平和脾细胞IL-2及IFN-诱导水平,MTT法检测兔脾细胞增殖水平.初步结果证实,pcD/IL-2基因佐剂可以显著加强pTpGpd疫苗在免疫期(0~8周)兔体内的特异性抗体及脾细胞增殖分化水平以及维持Tp感染后168天时间内长期稳定的高抗体水平及高脾细胞增殖分化水平.pcD/IL-2基因佐剂和CS纳米颗粒的联合使用对pcD/TpGpd疫苗的抗Tp皮肤感染的保护性效应达到最佳.
语种：
中文
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Neopterin negatively regulates expression of ABCA1 and ABCG1 by the LXR alpha signaling pathway in THP-1 macrophage-derived foam cells
作者：
Yan, Jin-quan;Tan, Chun-zhi* ;Wu, Jin-hua;Zhang, Dong-cui;Chen, Ji-ling;...
期刊：
Molecular and Cellular Biochemistry ,2013年379(1-2):123-131 ISSN：0300-8177
通讯作者：
Tan, Chun-zhi
作者机构：
[Yan, Jin-quan] Shaoyang Med Coll, Affiliated Hosp, Shaoyang 422000, Hunan, Peoples R China.;[Liu, Qin; Dai, Hao; Tan, Chun-zhi; Zeng, Bin-yuan; Liu, Wei; Jiang, Yu-ping; Nie, Jin] First Peoples Hosp Shaoyang City, Shaoyang 422000, Hunan, Peoples R China.;[Tan, Chun-zhi] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr, Hengyang 421001, Hunan, Peoples R China.;[Wu, Jin-hua] Nanhua Univ, Affiliated Hosp 2, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Dong-cui] Hunan Prov Geriatr Hosp, Changsha 410000, Hunan, Peoples R China.
通讯机构：
[Tan, Chun-zhi] F;First Peoples Hosp Shaoyang City, Shaoyang 422000, Hunan, Peoples R China.
关键词：
Neopterin;ABCA1;Cholesterol efflux;LXR alpha;Atherosclerosis
摘要：
To investigate the effects of neopterin on ABCA1 expression and cholesterol efflux in human THP-1 macrophage-derived foam cells, and to explore the role of the liver X receptor alpha (LXRα) involved. In the present study, THP-1 cells were pre-incubated with ox-LDL to become foam cells. The protein and mRNA expression were examined by Western blot assays and real-time quantitative PCR, respectively. Liquid scintillation counting and high performance liquid chromatography assays were used to test cellular cholesterol efflux and cholesterol content. Neopterin decreased ABCA1 expression and cholesterol efflux in a time- and concentration-dependent manner in THP-1 macrophage-derived foam cells, and the LXRα siRNA can reverse the inhibitory effects induced by neopterin. Neoterin has a negative regulation on ABCA1 expression via the LXRα signaling pathway, which suggests the aggravated effects of neopterin on atherosclerosis.
语种：
英文
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x连锁凋亡抑制蛋白介导二苯乙烯苷抗氧化诱导的人脐静脉内皮细胞凋亡
作者：
刘英;杨滢;李娜;仇梦霞;田英;...
期刊：
中国动脉硬化杂志 ,2013年21(9):I0019-I0019 ISSN：1007-3949
作者机构：
南华大学生物技术系生物化学与分子生物学教研室,湖南衡阳421001
会议名称：
第十二次全国动脉硬化性疾病学术会议
会议时间：
2013-10-11
会议地点：
江苏苏州
会议论文集名称：
第十二次全国动脉硬化性疾病学术会议论文集
关键词：
二苯乙烯苷;人脐静脉内皮细胞;细胞凋亡;X连锁凋亡抑制蛋白
摘要：
目的 研究二苯乙烯苷(TSG)对过氧化氢(H2O2)诱导的人脐静脉内皮细胞凋亡的作用以及TSG是否通过调节X连锁凋亡抑制蛋白(XIAP)的表达抗内皮细胞凋亡.方法 预先用不同剂量TSG(1～ 100 μmol/L)处理人脐静脉内皮细胞24h,再用300 μmol/L H2O2作用细胞24h.采用MTT法检测细胞活力,Hoeschest染色及流式细胞仪检测细胞凋亡.RT-PCR及Western blot检测TSG、XIAP抑制剂Embelin作用细胞后XIAP的表达.结果 300 μmol/L H2O2作用HUVECs细胞24 h可有效降低细胞活力,诱导细胞凋亡；1 ～ 100 μmol/L TSG预处理24 h均可提高细胞存活率,抑制细胞凋亡；30 μmol/L Embelin预处理细胞6h后再用TSG预处理24h,细胞凋亡率较TSG预处理组明显上升,细胞活力显著降低.RT-PCR、Western blot结果显示H2O2处理后,细胞XIAP表达水平显著降低；与H2O2组相比,1～ 100 μmol/L TSG预处理24h后,XIAP表达升高；与TSG预处理组相比,Embelin预处理6h后再用TSG预处理24h,XIAP的表达降低.结论 TSG能抑制H2O2诱导的人脐静脉内皮细胞凋亡,其作用机制与上调XIAP的表达有关.
语种：
中文
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