期刊论文

Li, J.

中文

药学学报

0513-4870

2013

48

11

1657-1664

10.16438/j.0513-4870.2013.11.011

国家自然科学基金资助项目（81202535,81173060） 湖南省科技厅资助项目（2013FJ3060） 湖南省教育厅资助项目（128117） 郴州市科技局资助项目（201216） 湘南学院科研基金资助项目（201261）.

本校为第一且通讯机构

探讨mmLDL对小鼠肠系膜动脉内皮依赖性舒张功能的影响及机制.采用离体微血管张力描记技术,透射电镜观察血管内皮超微结构.结果显示,mmLDL致小鼠肠系膜动脉内皮细胞明显水肿、内弹力膜断裂；与生理盐水组Rmax (63±5)％和pIC50 6.42± 0.09比较,显著抑制EDHF介导的舒张[Rmax(31±3)％和pIC50 5.67±0.07,P＜0.001,P＜0.001];与生理盐水组[Rmax(45±4)％和pICs0 5.93±0.08]比较,显著抑制NO介导的舒张[Rmax(32±4)％和pIC50 5.43±0.11,P＜0.05,P＜0.01]；对PGI2介导的舒张功能无明显影响.综上所述,mmLDL损伤内皮细胞超微结构;显著降低内皮依赖性舒张功能与NO-和EDHF途径受损有关.

This study is to investigate the impairment and possible mechanism of endothelium-dependent relaxation of mice mesenteric arteries induced by mmLDL. Wire myography was employed to examine endothelial function of mesenteric arteries. Ultramicrostructure of mesenteric vascular beds were detected by transmission electron microscope. The results showed that endothelium cell edema and peeling, vascular elastic membrane fracture traces in mmLDL group. Endothelium-dependent relaxation was decreased in a time-dependent and dose-dependent manner by using mmLDL, compared with normal arteries. In endothe...