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Effect of different wavelength ultraviolet radiation on photoreactivation in Vibrio parahaemolyticus

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成果类型:
期刊论文
作者:
苏泽红;何淑雅;李俐娟;练高建;高桥章
通讯作者:
Lian, G.-J.
作者机构:
[李俐娟; 何淑雅; 苏泽红] Department of Biotechnology, School of Pharmacology and Life Sciences and Technology, University of South China, Hengyang 421001, China
[Takahashi, Akira] Department of Preventive and Environment Nutrition, Tokushima University, Tokushima, 770-8501, Japan
[练高建] Department of Experimental Zoology, University of South China, Hengyang 421001, China
通讯机构:
Department of Experimental Zoology, University of South China, China
语种:
中文
关键词:
紫外照射;副溶血性弧菌;光修复反应;发光二极管
关键词(英文):
Light-emitting diodes;Photoreactivation;Ultra violet radiation;Vibrio parahaemolyticus
期刊:
现代食品科技
ISSN:
1673-9078
年:
2013
卷:
29
期:
12
页码:
2889-2893
基金类别:
湖南省自然科学基金项目(13JJ3081); 湖南省教育厅项目(12C0360); 南华大学博士启动基金项目(2011XQD54);
机构署名:
本校为第一且通讯机构
院系归属:
药学与生物科学学院
摘要:
低压水银管是常见的灭菌工具,其一般采用的是波长为254 nm的UVC作为灭菌光源.而UVA(365 nm波长)配合发光二极管(light-emitting diodes,LED)也有明显的杀菌效果.本研究从灭菌能力,照射后细菌的光修复能力和再生长能力以及能耗等方面入手,比较短波长的UVC和长波长的UVA/LED对副溶血性弧菌的灭菌效果.结果显示,UVA/LED比UVC有更强的杀菌能力,且在受到UVA/LED照射后,副溶血性弧菌的再生长明显慢于单纯UVC处理后的细菌;对副溶血性弧菌的光修复酶基因表达水平进行检测发现,UVA/LED照射后副溶血性孤菌三种光修复酶基因的表达水平都无激活现象,而UVC处理后的细菌三种基因表达水平明显升高.我们认为UVA/...
摘要(英文):
The low pressure mercury lamp is used as a common tool for disinfection, with wavelength 254 nm as light source. Previous results suggested that when connected with light-emitting diodes (LED), UVA could kill bacterial effectively. In this research, we found that the disinfection effect of UVA/LED was much stronger than that of UVC; meanwhile, the regeneration of Vibrio parahaemolyticus after radiated by UVA/LED was more slowly than that only exposed by UVC. The results showed that photoreactivation did not happen in Vibrio parahaemolyticus a...

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