期刊论文

中文

实验动物科学

1006-6179

2013

30

2

23-31+41

10.3969/j.issn.1006-6179.2013.02.006

湖南省自然科学基金项目（No.05JJ30046） 湖南省科研条件创新专项（No.2010TT1007）基金资助

本校为第一机构

目的探讨微卫星标记预警实验红鲫对苯酚遗传毒性的响应。方法选择5个微卫星标记,观察4个苯酚染毒组经8 d染毒实验,苯酚对实验红鲫C1HD系微卫星多态性的影响。结果苯酚在实验红鲫C1HD系体内的富集提高了基因杂合度（H）、基因多态性信息含量（PIC）等群体遗传学指数。在1/8 LC50苯酚浓度组染毒第6天,基因杂合度（H=0.15±0.19）和基因多态性信息含量（PIC=0.42）均达到峰值。结论体内富集一定浓度的苯酚可致实验红鲫C1HD系微卫星多态性改变。微卫星基因杂合度（H）和多态性信息含量（PIC）随苯酚染毒时间的延长和富集量的增加而升高。

Objective To study the microsatellite markers of early warning on the response of genotoxicity of phenol in red crucian carp C1HD. Method The red crucian carp C1HD were divided into 4 exposure groups and 1 control group and choose 5 microsatellite markers to study the effect of phenol on the microsatellite polymorphism after exposed to different concentration phenol for 8 d. Result The indexes were increased by the enrichment of phenol in red crucian carp C1HD, such as the gene heterozygosity （H）, gene polymorphism content （PIC）, and so on....