摘要：
A novel series of apigenin derivatives with phloroglucinol or resorcinol as raw materials were synthesized according to Baker-Venaktaraman reaction and their in vitro inhibitory activities on colorectal adenocarcinoma (HT-29) and leucocythemia (HL-60) cell lines were evaluated by the standard methyl thiazole tetrazolium (MTT) method. The results of biological test showed that some of apigenin derivatives possessed stronger anti-cancer activities than apigenin. Compound 6 showed the strongest activity against colorectal adenocarcinoma (HT-29) and leucocythemia (HL-60) cell lines with IC50 valure of 2.03±0.22 µM, 2.25±0.42 µM, it was better than 5-FU (12.92±0.61 µM, 9.56±0.16 µM), which shows a potential compound for colorectal adenocarcinoma and leucocythemia.
作者机构：
[REN Kun; WU Rong; LU Yan-Ju; YI Guang-Hui] Institute of Cardiovascular Research, Key Laboratory for Arteriosclerology of Hunan Province, University of South China
会议名称：
2014年第十二届全国脂质与脂蛋白学术会议
会议时间：
2014-10-10
会议地点：
杭州
会议主办单位：
中国生物化学与分子生物学会脂质与脂蛋白专业委员会
会议论文集名称：
2014年第十二届全国脂质与脂蛋白学术会议论文集
摘要：
<正>Apolipoprotein M(ApoM)is a plasma apolipoprotein that mainly associates with high-density lipoprotein(HDL).which is expressed by specific tissues and owns various biological functions.Plenty of fac
作者机构：
Department of Biochemistry, Institute of Biotechnology, University of South China, Hengyang, China;Department of Genetics, Institute of Life Science & Technology, Huazhong University of Science & Technology, Wuhan, China
关键词：
DNA Amplification;DNA Polymerase;Isothermal;Nickase;Strand Displacement
摘要：
We developed a nicking endonuclease dependent DNA amplification (NDA), using Nt.BstNBI to catalyze single-stranded nick on double-stranded DNA, and Bst DNA polymerase to make extension while sealing the nick and displacing the downstream strand. The displaced single-stranded DNA thereby serves as template for primers hybridization and extension, resulting in exponential synthesis of target DNA under isothermal condition. Over 105 folds target DNA amplification can be achieved in 30 minutes, generating DNA product suitable for both diagnosis and DNA cloning. This NDA strategy does not require thermal cycling or prerequisite nucleotides modification, making it suitable for application in the field and at the point-of-care.
作者：
Li Guoqing;Xiao Zhefeng;Li Maoyu;Peng Fang;Hu Rong;...
作者机构：
[Li Guoqing; Xiao Zhefeng; Li Maoyu; Peng Fang; Hu Rong; Shao Meiying; Chen Xiaojuan; Li Yuanyuan; Huang Ying; Zhan Xianquan; Chen Zhuchu] State Local Joint Engineering Laboratory for Anticancer Drugs,Hunan Engineering Laboratory for Structural Biology&Drug Design,Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Xiangya Hospital,Central South University,Changsha,P.R.China,410008;[Li Guoqing] Department of Biology,School of Pharmacy and Life Science,University of South China,Hengyang,Hunan,P.R.China,410008;[Li Yuanyuan] Medical College,Guangxi University of Science and Technology,Liuzhou,Guangxi,P.R.China,545005
会议名称：
第八届中国蛋白质组学大会
会议时间：
2013-9-7
会议地点：
重庆
会议主办单位：
中国生物化学与分子生物学会
会议论文集名称：
第八届中国蛋白质组学大会论文集
关键词：
Nasopharyngeal Carcinoma;Formalin-fixed;paraffin-embedded;isobaric tags for relative and absolute quantification;Different Stages;Ingenuity pathway analysis
摘要：
Aim To determine the role of receptor component protein (RCP) in calcitonin gene-related peptide (CGRP) and angiotensin Ⅱ signal transduction in action of vascular peroxidase-1 (VPO1) in VSMC.Methods Mouse vascular smooth muscle cell line A10 (A10VSMC) was employed in the study.Western blot was used to detect the expression of RCP and VPO1 protein.RT-PCR was used to detect the expression of RCP and VPO1 mRNA.RCP-specific small interference RNA (RCP-siRNA) was used to silence oligonucleotide sequence co-immunoprecipitation was used to determine interaction of RCP and Gαs/Gβ/Gγ Results Both CGRP and angiotensin Ⅱ up-regulated the expression of RCP while CGRP down-regulated the expression of RCP induced by angiotensin Ⅱ in VSMC;Angiotensin Ⅱ up-regulated the expression of VPO1 in a concentration and time-dependent manner in VSMC;CGRP inhibited the expression of VPO1 induced by angiotensin Ⅱ and the effect of CGRP was enhanced by Catalase, H2O2 scavenger.RCP-siRNA successfully decreased RCP protein expression by ~80% in 48 h, following with an decrease in VPO1 expression in CGRP and/or angiotensin Ⅱ induced VSMC In addition, RCP co-immunoprecipitated with Gαs and Gβ but not with Gγ in VSMC.Conclusion RCP may contribute to the inhibitory effect of CGRP on angiotensin Ⅱ induced VPO1 expression in VSMC, and the mechanism may be related to the interactions between RCP and Gαs / Gβ.
作者：
YAN GLi;SU Tao;LV De-Guan;XIE Feng;LIU Wei;...
作者机构：
[YAN GLi; SU Tao; LV De-Guan; XIE Feng; LIU Wei; CAO Jian-Gang; QIN Xu-Ping; LI Lan-Fang; CHEN Lin-Xi] Institution of Pharmacy and Pharmacology,University of South China,Learning Key Laboratory for Pharmaco-proteomics;[YAN GLi; SU Tao; LV De-Guan; XIE Feng; LIU Wei; CAO Jian-Gang; QIN Xu-Ping; LI Lan-Fang; CHEN Lin-Xi] Pharmaceutical Department,Jingzhou First People’s Hospital;[YAN GLi; SU Tao; LV De-Guan; XIE Feng; LIU Wei; CAO Jian-Gang; QIN Xu-Ping; LI Lan-Fang; CHEN Lin-Xi] First Affiliated Hospital of Yangtze University
会议名称：
2013医学前沿论坛暨第十三届全国肿瘤药理与化疗学术会议
会议时间：
2013-5-5
会议地点：
洛阳
会议主办单位：
中国药理学会;中国抗癌协会;中国工程院医药卫生学部
会议论文集名称：
2013医学前沿论坛暨第十三届全国肿瘤药理与化疗学术会议论文集
关键词：
Apelin;A549;ERK1/2;proliferation;autophagy
摘要：
<正>The aim of the study was to investigate the role that apelin has played in the proliferation and autophagy of lung adenocarcinoma.Bioinformatics analysis revealed the distribution of APJ in various tissues and whether or not APJ exist in lung adenocarcinoma cell line A549.0verexpression of APJ in lung adenocarcinoma was detected by immunohistochemistry
作者机构：
[Lv Deguan; Lu Qixuan; Li Yao; Cao Jianguang; Yang Lingfang; Liu Meiqing; Zhang Hanjing; Qin Xuping; Li Lanfang; Chen Linxi] Institute of pharmacy and pharmacology,University of South China,Hengyang,Hunan,421001
摘要：
<正>Background and objective:Apelin is a newly discovered bioactive peptides which had been proved to be an endogenous ligand of the APJ receptor.Recent evidences had confirmed that apelin is a novel c
