Aim To determine the role of receptor component protein (RCP) in calcitonin gene-related peptide (CGRP) and angiotensin Ⅱ signal transduction in action of vascular peroxidase-1 (VPO1) in VSMC.Methods Mouse vascular smooth muscle cell line A10 (A10VSMC) was employed in the study.Western blot was used to detect the expression of RCP and VPO1 protein.RT-PCR was used to detect the expression of RCP and VPO1 mRNA.RCP-specific small interference RNA (RCP-siRNA) was used to silence oligonucleotide sequence co-immunoprecipitation was used to deter...
