期刊论文

中文

中国实验动物学报

1005-4847

2013

21

2

64-67

10.3969/j.issn.1005-4847.2013.02.013

【基金项目】湖南省教育厅项目资助（12C0360）.

本校为第一机构

目的寻找能调节T细胞功能的相关分子,进行与T细胞介导的自身免疫性疾病相关的研究。方法收集BALB/c小鼠脾细胞,免疫Wistar大鼠,进行细胞融合,建立杂交瘤细胞系。筛选得到43株能调节T细胞功能的杂交瘤细胞系,对其中一株最能抑制T细胞增殖的杂交瘤细胞系进行了进一步的深入研究。结果显示其目标分子是CD98重链,同时后续实验显示抗CD98单克隆抗体能抑制纤连蛋白介导的细胞分布,但不影响氨基酸转运。而且混合淋巴细胞反应显示该抗体能显著抑制T细胞增殖反应。结论抗CD98单克隆抗体能有效抑制T细胞增殖,有望将本抗体用于T细胞介导的自身免疫性疾病的相关预防及治疗中。

Objective To identify molecules that modulate T-cell functions and serve the studies on T-cell media- ted autoimmune diseases. Methods We collected splenoeytes from BALB/c mice to immunize Wistar rats and to establish many hybridoma cell lines. Forty-three hyhridoma cell lines which could modulate T-cell functions were obtained. One of the 43 cell lines, most actively inhibiting T-cell proliferation, was further studied. Results The monoclonal antibody anti- CD98hc mAb recognized CD98 heavy chain, and suppressed fihronectin-mediated cell spreading hut not amino acid trans- port. Furthermore, t...