作者机构：
[Qinhui Tuo; duanfang Liao] Hunan University of Chinese Medicine, 300＃ Xueshi Rd, Hanpu District of Science and Education;[Qinhui Tuo; Shuhua Cui; Tianping Li; Juan Wen] Key Laboratory for Pharmacoproteomics, School of Life Science and Technology, University of South China;[Guozuo Xiong] Department of general surgery, the second affiliated hospital in University of South China
会议名称：
脂质组学与脂蛋白的新功能-第十二届全国脂质与脂蛋白学术会议
会议时间：
2014-01-01
会议地点：
中国浙江杭州
会议主办单位：
中国生物化学与分子生物学会脂质与脂蛋白专业委员会
会议论文集名称：
第十二届全国脂质与脂蛋白学术会议论文汇编
摘要：
<正>To explore whether Daxx mediates Chol:MβCD induced cholesterol accumulation and apopotosis in macrophage.The eukaryotic vector of Daxx was constructed to be stably transfected into RAW264.7 cells,the mRNA and protein expressions of Daxx was quantified by RT-PCR and western blotting,respectively.Intracellular lipiddroplets and lipid levels were assayed by oil red Q staining and enzymefluorescent analysis.The growth inhibition
期刊：
Lecture Notes in Electrical Engineering,2014年269(4):3065-3070 ISSN：1876-1100
通讯作者：
Guo, Z.(guozifen@aliyun.com)
作者机构：
[Huang, Honglin; Guo, Zifen] Department of Pharmacy, University of South China, Hengyang 421001, China;[Feng, Yong] Student Affairs Department, University of South China, Hengyang 421001, China
作者机构：
[苏琦; 谭晖; 何洁; Shan J.] Cancer Research Institute, First Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China;[李林蔚] College of Pharmacy and Biological Sciences, First Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China;[伍尤华] Dept of Oncology, First Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China;[易岚] Cancer Research Institute, First Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China, College of Pharmacy and Biological Sciences, First Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China
通讯机构：
[Yi, L.] C;Cancer Research Institute, First Affiliated Hospital, University of South China, Hengyang Hunan, China
摘要：
Proliferation of vascular smooth muscle cells (VSMCs) contributes to the development of atherosclerosis. Ezetimibe is a new lipid lowering agent that inhibits cholesterol absorption. In the present study we attempted to investigate whether ezetimibe has any effect on VSMC proliferation and the potential mechanisms involved. Our data showed ezetimibe abrogated the proliferation and migration of primary rat VSMCs induced by Chol:MβCD. Mechanically, we found that ezetimibe was capable of abolishing cyclin D1, CDK2, phospho-Rb (p-Rb), and E2F protein expressions that were upregulated by Chol:MβCD treatment. In addition, Ezetimibe was able to reverse cell cycle progression induced by Chol:MβCD, which was further supported by its down-regulation of cyclin D1 promoter activity in the presence of Chol:MβCD. Furthermore, ezetimibe abrogated the increment of phospho-ERK1/2 (p-ERK1/2) and nuclear accumulation of ERK1/2 in VSMCs induced by Chol:MβCD. Inhibition of the MAPK pathway by using ERK1/2 inhibitor PD98059 attenuated the reduction effect of ezetimibe on the expressions of phosphor-MEK1 (p-MEK1), p-ERK1/2, and cyclin D1. Taken together our data suggest that ezetimibe inhibits Chol:MβCD-induced VSMCs proliferation and leads to cell cycle arrest at the G0/G1 phase by suppressing cyclin D1 expression via the MAPK signaling pathway. These novel findings support the potential pleiotropic effect of ezetimibe in cardiovascular disease.
作者机构：
[Zhong-Cheng Mo; Ji Xiao; Zhi-feng Long; Guang-hui Yi; Feng Yao; Yu-lin Tan; Min Zhang; Guo-jun Zhao; Chao-ke Tang] Institute;[Zhong-Cheng Mo; Ji Xiao; Zhi-feng Long; Guang-hui Yi; Feng Yao; Yu-lin Tan; Min Zhang; Guo-jun Zhao; Chao-ke Tang] of;[Zhong-Cheng Mo; Ji Xiao; Zhi-feng Long; Guang-hui Yi; Feng Yao; Yu-lin Tan; Min Zhang; Guo-jun Zhao; Chao-ke Tang] Cardiovascular;[Zhong-Cheng Mo; Ji Xiao; Zhi-feng Long; Guang-hui Yi; Feng Yao; Yu-lin Tan; Min Zhang; Guo-jun Zhao; Chao-ke Tang] Research,;[Zhong-Cheng Mo; Ji Xiao; Zhi-feng Long; Guang-hui Yi; Feng Yao; Yu-lin Tan; Min Zhang; Guo-jun Zhao; Chao-ke Tang] Key
会议名称：
2014年第十二届全国脂质与脂蛋白学术会议
会议时间：
2014-10-10
会议地点：
杭州
会议主办单位：
中国生物化学与分子生物学会脂质与脂蛋白专业委员会
会议论文集名称：
2014年第十二届全国脂质与脂蛋白学术会议论文集
摘要：
<正>Objects:The aim of this study is to investigate the effects of advanced oxidation protein products(AOPPs)on ATP-binding cassette transporter A1(ABCA1)and ABCG1 expression,atherosclerotic lesion and
摘要：
This study is to investigate the expression of miR-21 in nasopharyngeal carcinoma (NPC) cells, and the effect of miR-21 in the biological behavior and expression of B-cell lymphoma 2 (BCL2) in NPC cells. Paired NPC and adjacent non-tumor tissues were obtained from 53 patients who underwent primary surgical resection of NPC tissues. Luciferase reporter assay was performed to test whether BCL2 is a direct target of miR-21. Methylthiazolyl blue tetrazolium assay and colony assay were used to evaluate the effect of miR-21 on NPC cell proliferation. Transwell and wound-healing assays were carried out to test the effect of low expression of miR-21 on cancer cell migration and invasion. QRT-PCR and Western blotting were used to measure the levels of mRNA and protein expression, respectively. Tumor tissues showed a positive correlation between the levels of miR-21 and BCL2 protein expression. Cells transfected with miR-21 inhibitor healed slower compared the control (P < 0.05). In addition, cell migration was notably inhibited by the down-regulation of miR-21 in vitro (P < 0.05). The reduction in miR-21 expression showed a remarkable effect on the biological behavior of NPC cell clone formation (P < 0.05). Low expression of miR-21 by transfection with miRNA expression plasmid led to a decrease in BCL2 expression, which was accompanied by reduced migration and proliferation of the cancer cells. Our results demonstrated that miR-21 inhibitor down-regulated BCL2 expression level, suggesting that BCL2 might be a target gene for the initiation and development of NPC cells.
作者机构：
[Rongbin Huang; Xiang Lei; Xuan Cao; Lanfang Li; Guotao Tang] Institute of Pharmacy and Pharmacology,University of South China,Hengyang,Hunan,421001,People's Republic of China
摘要：
<正>pH-sensitive amphiphilic poly(ethylene glycol)-imine-benzoic-dipalmitate(PEG-I-dC16)polymers with two different PEGs molecular weight were designed and synthesized.The molecular structures of the p
