期刊论文

Yi Guang-Hui

中文

生物化学与生物物理进展

1000-3282

2014

41

7

674-681

10.3724/SP.J.1206.2013.00357

(81270360,81100211):国家自然科学基金资助项目 (2013SK3114):湖南省科技计划资助项目 (南华大学基础医学学科,湘教发[2011]76号):湖南省重点学科建设项目 (81270360,81100211):This work was supported by grants from The National Natural Sciences Foundation of China (2013SK3114) and The Construct Program of The Key Discipline in Hunan Province (Basic Medicine Sciences in University of South China):The Science and Technology Plan Projects of Hunan Province

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为探讨过氧化物酶体增殖物激活型受体δ(peroxisome proliferator- activated receptor-δ,PPARδ)激动剂GW501516对人脐静脉内皮细胞纤溶酶原激活物抑制剂1 (PAI-1)表达的影响及机制,采用siRNA、TGFβ-Smad3信号通路阻滞剂等处理细胞,经实时定量PCR、Western blot 方法分别检测细胞中PAI-1及磷酸化Smad3蛋白的表达.结果显示,与对照组比较, GW501516可诱导人脐静脉内皮细胞(HUVEC)中PAI-1表达,且此效应呈浓度和时间依赖性(P < 0.05);siRNA 沉默PPARδ的表达后,可阻抑GW501516对HUVEC细胞PAI-1表达的促进作用;TGFβ-Smad3信号通路抑制剂SB-431542与SIS3均可降低HUVEC细胞pSmad3蛋白的表达,而细胞...

Increased plasminogen activator inhibitor-1 (PAI-1) level is the risk of thrombotic disease and atherosclerosis (As). Our research aims to study the effect and mechanism of PPAR delta antagonist GW501516 on the expression of PAI-1. Firstly, human umbilical vein endothelial cells (HUVECs) were incubated with DMEM, and then treated with siRNA and TGF beta-Smad3 inhibitor, respectively. The protein and mRNA expression were examined by Western blotting assays and Real-time quantitative PCR, respectively. The result showed that GW501516 induced PAI-1 mRNA and protein expression in HUVECs compared w...