National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30800996]; Science and Technology Department Key Foundation of Hunan Province in China [2010FJ2008]; National Natural Science Foundation of Hunan Province in China [11JJ9023]; construct program of the key discipline in the Hunan province
机构署名：
本校为第一且通讯机构
院系归属：
医学院
药学与生物科学学院
摘要：
Syphilis is a chronic infection caused by Treponema pallidum subsp. pallidum, and diagnosis with sensitive and specific methods is a challenging process that is important for its prevention and treatment. In the present study, we established a recombinant protein TpF1-based indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) and a Western blot assay for human and rabbit sera. The 20-kDa recombinant protein TpF1 was detected by Western blotting performed with sera from rabbits immunized with recombinant TpF1 and infected wi...
