The VOCM content determination method was established, and the steam distillation extraction conditions, such as some powder thickness, extraction solvent, immersion, etc. were optimized, in order to determine the optimum extracting method. Extracting of volatile oil from centipede by using steam distillation, and the content of volatile oil was determined by the volatilization curve method. The optimal process of the steam distillation was that the powder was soaked one or two hours, and then extracted by the steam distillation and extraction with ether. Evaporation curve method RSD was 0. 95...