Sorting nexins: A novel promising therapy target for cancerous/neoplastic diseases
作者：
Yang, Lu;Tan, Weihua;Yang, Xinzhi;You, Yong;Wang, Jing;...
期刊：
JOURNAL OF CELLULAR PHYSIOLOGY ,2021年236(5):3317-3335 ISSN：0021-9541
通讯作者：
Wen, Gebo;Zhong, Jing
作者机构：
[Wen, Gebo; Yang, Xinzhi; Zhong, Jing; Yang, Lu; Wen, GB; Zhong, J] Univ South China, Canc Res Inst, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Hengyang 421001, Hunan, Peoples R China.;[Wen, Gebo; Yang, Xinzhi; Zhong, Jing; Yang, Lu; Tan, Weihua] Univ South China, Affiliated Hosp 1, Inst Clin Med, Hengyang, Hunan, Peoples R China.;[Tan, Weihua] Univ South China, Affiliated Hosp 1, Emergency Dept, Hengyang, Hunan, Peoples R China.;[You, Yong; Wang, Jing] Univ South China, Hengyang Med Coll, Res Lab Translat Med, Hengyang, Hunan, Peoples R China.
通讯机构：
[Wen, GB; Zhong, J] U;Univ South China, Canc Res Inst, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Hengyang 421001, Hunan, Peoples R China.
关键词：
snx1 protein;snx10 protein;snx2 protein;snx27 protein;snx3 protein;snx5 protein;snx6 protein;snx9 protein;sorting nexin;tumor marker;unclassified drug;endocytosis;endosome;human;intracellular signaling;intracellular transport;malignant neoplasm;nonhuman;priority journal;protein domain;protein family;protein function;protein targeting;Review
摘要：
Sorting nexins (SNXs) are a diverse group of cytoplasmic- and membrane-associated phosphoinositide-binding proteins containing the PX domain proteins. The function of SNXproteins in regulating intracellular protein trafficking consists of endocytosis, endosomal sorting, and endosomal signaling. Dysfunctions of SNXproteins are demonstrated to be involved in several cancerous/neoplastic diseases. Here, we review the accumulated evidence of the molecular structure and biological function of SNXproteinsand discuss the regulatory role of SNXproteins in distinct cancerous/neoplastic diseases. SNXfamily proteins may be a valuable potential biomarker and therapeutic strategy for diagnostics and treatment of cancerous/neoplastic diseases. © 2020 Wiley Periodicals LLC
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英文
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Corrigendum to “Diallyl disulfide suppresses proliferation and induces apoptosis in human gastric cancer through Wnt-1 signaling pathway by up-regulation of miR-200b and miR-22” [Canc. Lett. 340 (1) (Oct 28, 2013) 72–81]
作者：
Tang, Hailin;Kong, Yanan;Guo, Jiaoli;Tang, Yi;Xie, Xinhua;...
期刊：
Cancer Letters ,2021年498:253 ISSN：0304-3835
通讯作者：
Su, Qi;Xie, Xiaoming
作者机构：
[Tang, Hailin; Xie, Xinhua; Yang, Lu; Xie, Xiaoming; Kong, Yanan; Guo, Jiaoli] Sun Yat Sen Univ, Ctr Canc, Dept Breast Oncol, Guangzhou, Guangdong, Peoples R China.;[Tang, Hailin; Xie, Xinhua; Yang, Lu; Xie, Xiaoming; Kong, Yanan; Guo, Jiaoli] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol South China, Guangzhou, Guangdong, Peoples R China.;[Tang, Hailin; Su, Qi; Tang, Yi] Univ South China, Canc Res Inst, Hengyang, Hunan, Peoples R China.;[Su, Q; Xie, XM] Sun Yat Sen Univ, Dept Breast Oncol, State Key Lab Oncol South China, Ctr Canc, 651East Dongfeng Rd, Guangzhou 510060, Peoples R China.
通讯机构：
[Su, Q; Xie, XM] S;Sun Yat Sen Univ, Dept Breast Oncol, State Key Lab Oncol South China, Ctr Canc, 651East Dongfeng Rd, Guangzhou 510060, Peoples R China.
摘要：
We are extremely sad to find that there is a mistake in this article. Due to our mistake, the IHC images of Wnt1 and TCF-4 in the Scramble group and the Mock group have been uploaded mistakenly (Fig. 6A and B). We really apologize for the inconvenience (The original version of the pictures are attached). [Table presented] Apologize for the inconvenience again. We hope that the corrigendum could be acceptable. “suqi1945@yahoo.com.cn” has been changed “suqi1945@163.com”. © 2020 Elsevier B.V.
语种：
英文
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医患纠纷案例大数据在医学人文素质教育中的应用策略
作者：
游咏;莫靓;唐志晗;杨璐;王婧;...
期刊：
医学教育管理 ,2020年6(5):457-460 ISSN：2096-045X
作者机构：
南华大学衡阳医学院医学人文教研室;南华大学附属第一医院;[王婧; 唐志晗; 杨璐; 姜志胜; 游咏; 陈代娣] 南华大学;[莫靓; 刘江华; 陈国栋] 南华大学第一附属医院
关键词：
医患纠纷;大数据;医学人文;素质教育
摘要：
新型冠状病毒肺炎(以下简称新冠肺炎)疫情对医学教育带来了挑战,同时也带来新的机遇.具有针对性与时效性的医学人文素质教育对于提高医学生人文素养,促进医学生养成良好的医患沟通能力,减少因沟通不畅诱发医患矛盾冲突,保障医疗安全,构建和谐医患关系有着及其重要的作用.通过对构建基于医患纠纷案例大数据的医学人文素质教育优化体系的实施策略进行探讨,以期为医学人文素质教育改革提供参考.
语种：
中文
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大数据应用于医学人文素质教育的研究现状分析
作者：
游咏;张新华;杨璐;王婧;唐志晗;...
期刊：
教育教学论坛 ,2020年(21):232-234 ISSN：1674-9324
作者机构：
南华大学衡阳医学院医学人文教研室,湖南衡阳421001;南华大学附属第一医院胸外科,湖南衡阳421001;[王婧; 唐志晗; 杨璐; 游咏; 张新华] 南华大学;[莫靓] 南华大学第一附属医院
关键词：
大数据;医学人文素质教育;优化体系;健康中国
摘要：
数据和知识的转化与重组使大数据逐渐成为促进医学和人类健康卫生事业发展的重要手段和方向。该文通过分析大数据与医学人文素质教育的研究现状,思考大数据对医学人文素质教育研究的重要意义,为深化医学人文素质教育改革、优化教育体系、提升实效提供研究途径,为健康中国建设输送一流医学人才提供研究支撑。
语种：
中文
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Hepatocyte nuclear factor 4 alpha negatively regulates connective tissue growth factor during liver regeneration
作者：
Zhou, Junmei;Sun, Xiaowei;Yang, Lu;Wang, Liqun;Ran, Gai;...
期刊：
FASEB JOURNAL ,2020年34(4):4970-4983 ISSN：0892-6638
通讯作者：
Ling, Chen;Pi, Liya
作者机构：
[Ran, Gai; Ling, Chen; Zhou, Junmei; Pi, Liya; Sun, Xiaowei] Univ Florida, Dept Pediat, Gainesville, FL USA.;[Zhou, Junmei] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang, Peoples R China.;[Sun, Xiaowei] Lanzhou Univ, Inst Pathol, Sch Basic Med Sci, Lanzhou, Peoples R China.;[Yang, Lu; Wang, Jinhui] City Hope Natl Med Ctr, Integrat Genom Core, Beckman Res Inst, Duarte, CA USA.;[Bryant, Andrew; Wang, Liqun] Univ Florida, Dept Med, Gainesville, FL USA.
通讯机构：
[Ling, Chen] F;[Pi, Liya] U;Fudan Univ, Zhongshan Hosp, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200438, Peoples R China.;Univ Florida, Coll Med, Dept Pediat, 1200 S Newell Dr, Gainesville, FL 32610 USA.
关键词：
connective tissue growth factor (Ctgf);hepatocyte nuclear factor 4α (Hnf4α);liver injury;liver regeneration
摘要：
Liver regeneration after injury requires fine-tune regulation of connective tissue growth factor (Ctgf). It also involves dynamic expression of hepatocyte nuclear factor (Hnf)4α, Yes-associated protein (Yap), and transforming growth factor (Tgf)-β. The upstream inducers of Ctgf, such as Yap, etc, are well-known. However, the negative regulator of Ctgf remains unclear. Here, we investigated the Hnf4α regulation of Ctgf post-various types of liver injury. Both wild-type animals and animals contained siRNA-mediated Hnf4α knockdown and Cre-mediated Ctgf conditional deletion were used. We observed that Ctgf induction was associated with Hnf4α decline, nuclear Yap accumulation, and Tgf-β upregulation during early stage of liver regeneration. The Ctgf promoter contained an Hnf4α binding sequence that overlapped with the cis-regulatory element for Yap and Tgf-β. Ctgf loss attenuated inflammation, hepatocyte proliferation, and collagen synthesis, whereas Hnf4α knockdown enhanced Ctgf induction and liver fibrogenesis. These findings provided a new mechanism about fine-tuned regulation of Ctgf through Hnf4α antagonism of Yap and Tgf-β activities to balance regenerative and fibrotic signals. © 2020 Federation of American Societies for Experimental Biology
语种：
英文
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“医患矛盾”大数据与医学人文素质教育的交融机制探讨
作者：
游咏;唐志晗;杨璐;王婧;莫靓
期刊：
继续医学教育 ,2020年34(9):52-54 ISSN：1004-6763
作者机构：
南华大学衡阳医学院医学人文教研室,湖南 衡阳 421001;南华大学附属第一医院胸外科,湖南 衡阳 421001;[王婧; 唐志晗; 杨璐; 游咏] 南华大学;[莫靓] 南华大学第一附属医院
关键词：
医患矛盾;大数据;医学人文;素质教育;机制
摘要：
现代信息技术运用于医学教育教学已逐渐成为医学教育改革的重要发展趋势。在当前医疗环境下,医患矛盾呈现易变性和复杂性的特点,需要医学人文素质教育更具针对性与时效性,促进教学目标、课程体系、教学内容、方式方法、资源环境不断优化。"医患矛盾"大数据的充分挖掘与全面深入分析将为医学人文素质教育提供新的改革方向。文章拟通过对大数据与医学人文素质教育交融的时代背景进行分析,探讨"医患矛盾"大数据与医学人文素质教育的交融机制,为医学人文素质教育改革向数字化、信息化、多学科交叉融合改造升级提供新的研究方向。
语种：
中文
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FGF21 protects human umbilical vein endothelial cells against high glucose-induced apoptosis via PI3K/Akt/Fox3a signaling pathway
作者：
Guo, Dongmin;Xiao, Lele;Hu, Huijun;Liu, Mihua;Yang, Lu* ;...
期刊：
Journal of Diabetes and its Complications ,2018年32(8):729-736 ISSN：1056-8727
通讯作者：
Lin, Xiaolong;Yang, Lu
作者机构：
[Guo, Dongmin; Yang, Lu] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang City 421001, Hunan, Peoples R China.;[Hu, Huijun; Lin, Xiaolong] Guangzhou Med Univ, Huizhou Peoples Hosp 3, Dept Pathol, Huizhou City 516001, Guangdong, Peoples R China.;[Xiao, Lele] Huzhou Univ, Huzhou City 313000, Zhejiang, Peoples R China.;[Liu, Mihua] Chongqing Med Univ, Affiliated Hosp 2, Ctr Lipid Res, Chongqing 400016, Peoples R China.;[Liu, Mihua] Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis,Minist Educ, Key Lab Mol Biol Infect Dis,Dept Infect Dis, Chongqing 400016, Peoples R China.
通讯机构：
[Lin, Xiaolong] G;[Yang, Lu] U;Guangzhou Med Univ, Huizhou Peoples Hosp 3, Dept Pathol, Huizhou City 516001, Guangdong, Peoples R China.;Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang City 421001, Hunan, Peoples R China.
关键词：
*Akt;*Apoptosis;*FGF21;*FoxO3a;*Oxidative stress;*eNOS
摘要：
Aims: Diabetic macroangiopathy is the main cause of morbidity and mortality in patients with diabetes. Endothelial cell injury is a pathological precondition for diabetic macroangiopathy. Fibroblast growth factor 21 (FGF21) is a key metabolic regulator which has recently been suggested to protect cardiac myocytes and vascular cells against oxidative stress-induced injury in vitro and vivo. In this study, we aimed to investigate the protective capacity of FGF21 in human umbilical vein endothelial cells (HUVECs) against high glucose (HG)-induced apoptosis via phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt)/FoxO3a pathway. Methods: The cell viability was examined by CCK-8 assay, Intracellular ROS levels were measured by the detection of the fluorescent product formed by the oxidation of DCFH-DA, Apoptosis was analyzed using Hoechst 33258 nuclear staining and Flow Cytometry Analysis (FCA), the expression of protein were detected by Western blot. Results: Results show that pretreating HUVECs with FGF21 before exposure to HG increases cell viability, while decreasing apoptosis and the generation of reactive oxygen species. Western blot analysis shows that HG reduces the phosphorylation of Akt and FoxO3a, and induces nuclear localization of FoxO3a. The effects were significantly reversed by FGF21 pre-treatment. Furthermore, the protective effects of FGF21 were prevented by PI3K/Akt inhibitor LY294002. Conclusions: Our data demonstrates that FGF21 protects HUVECs from HG-induced oxidative stress and apoptosis via the activation of PI3K/Akt/FoxO3a signaling pathway. (C) 2018 Elsevier Inc. All rights reserved.
语种：
英文
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Involvement of CGRP-RCP in the caveolin-1/ERK1/2 signal pathway in the static pressure-induced proliferation of vascular smooth muscle cells
作者：
Guo, Feng;Yang, Li;Luo, Jingfei;Quan, Haiyan;Wang, Zhen;...
期刊：
JOURNAL OF CELLULAR PHYSIOLOGY ,2018年233(10):6910-6920 ISSN：0021-9541
通讯作者：
Zhang, Liang;Qin, Xuping
作者机构：
[Luo, Jingfei; Wang, Zhen; Li, Jie; Qin, Xuping; Yang, Li; Jiang, Zhisheng; Guo, Feng; Quan, Haiyan; Peng, Hongyan; Hong, Chenliang] Univ South China, Inst Pharm & Pharmacol, Key Lab Arteriosclerol Hunan Prov, Hengyang, Peoples R China.;[Zhang, Liang] Palmer Coll Chiropract, Palmer Lab Cell & Mol Biol, Port Orange, FL 32129 USA.;[Qin, Xuping] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
通讯机构：
[Zhang, Liang] P;[Qin, Xuping] U;Palmer Coll Chiropract, Palmer Lab Cell & Mol Biol, Port Orange, FL 32129 USA.;Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
关键词：
calcitonin gene related peptide;caveolin 1;cycline;messenger RNA;mitogen activated protein kinase 3;receptor component protein;receptor protein;small interfering RNA;unclassified drug;calcitonin gene related peptide;caveolin 1;cycline;mitogen activated protein kinase;mitogen activated protein kinase 3;animal cell;Article;caveola;cell growth;cell proliferation;cell viability;controlled study;gene silencing;mechanotransduction;membrane microdomain;mouse;nonhuman;pressure;priority journal;protein expression;signal transduction;static pressure;vascular smooth muscle cell;animal;cell culture;cell line;cell proliferation;drug effect;MAPK signaling;metabolism;physiology;smooth muscle cell;vascular smooth muscle;Animals;Calcitonin Gene-Related Peptide;Caveolin 1;Cell Line;Cell Proliferation;Cells, Cultured;MAP Kinase Signaling System;Mice;Mitogen-Activated Protein Kinase 3;Mitogen-Activated Protein Kinases;Muscle, Smooth, Vascular;Myocytes, Smooth Muscle;Proliferating Cell Nuclear Antigen
摘要：
Previous study suggested that the receptor component protein (RCP), one of the components of calcitonin gene-related peptide (CGRP) receptor, plays a multiple role in the cellular signal transduction. The study was designed to investigate whether or not the RCP involved in the regulation of caveolin-1/extracellular signal-regulated kinases-1 and -2 (ERK1/2) signal pathway in the vascular smooth muscle cells (VSMCs) proliferation induced by static pressure. Mouse-derived VSMCs line A10 (A10 VSMCs) was served as project in this experiment. Results showed that the A10 VSMCs viability and proliferating cell nuclear antigen (PCNA) expression which were increased by static pressure were inhibited by pretreatment of CGRP. In like manner, the expressions of the decreased-caveolin-1 and the increased-phosphorylated ERK1/2 (p-ERK1/2) induced by static pressure were significantly reversed by pretreatment of CGRP, respectively. Meanwhile, the expression of RCP was up-regulated by the static pressure. Silence of RCP gene with the small interrupt RNA (siRNA) not only significantly increased A10 VSMC proliferation but also increased the expression of p-ERK1/2 in response to static pressure. When treatment of A10 VSMCs with 120-mmHg static pressure for different time, however, the protein band of caveolin-1 and RCP was the least at time point of 10 min, but the p-ERK1/2 expression was the most maximum. In conclusion, RCP maybe involved in the static pressure-induced A10 VSMCs proliferation by regulation of caveolin-1/ERK1/2 signal pathway. © 2018 Wiley Periodicals, Inc.
语种：
英文
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多模式信息化教学运用于诊断学教学中的思考与探索
作者：
游咏;李熠;尹凯;杨璐;桂庆军
期刊：
教育现代化 ,2018年5(48):190-191 ISSN：2095-8420
作者机构：
南华大学医学院诊断学教研室,湖南 衡阳;南华大学医学人文教研室,湖南 衡阳;[李熠; 杨璐; 桂庆军; 尹凯; 游咏] 南华大学
关键词：
模式;信息化;诊断学;教学
摘要：
诊断学是医学生必修的一门重要专业课程.如何在诊断学教学过程中选择与构建优化的教学模式与方法,提高教学效果,提升学生的学习兴趣是目前各高校诊断学教学研究的主要方向.本文根据诊断学各章节教学内容的特点,探讨将微课、虚拟仿真以及翻转课堂等信息化教学方式有机运用于诊断学的教学中,有效地促进诊断学教学改革.以期为学生提供丰富的教学资源,提供直观、逼真的学习环境,提高学生动手的机会,锻炼学生的临床思维,调动学生的学习积极性,达到提升诊断学教学质量的目的.
语种：
中文
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PDGF Promotes the Warburg Effect in Pulmonary Arterial Smooth Muscle Cells via Activation of the PI3K/AKT/mTOR/HIF-1 alpha Signaling Pathway
作者：
Xiao, Yunbin;Peng, Hongyan;Hong, Chenliang;Chen, Zhi;Deng, Xicheng;...
期刊：
Cellular Physiology and Biochemistry ,2017年42(4):1603-1613 ISSN：1015-8987
通讯作者：
Qin, Xuping
作者机构：
[Xiao, Yunbin] Univ South China, Acad Pediat, Hengyang, Peoples R China.;[Chen, Zhi; Xiao, Yunbin; Deng, Xicheng] Hunan Childrens Hosp, Dept Cardiol, Changsha, Hunan, Peoples R China.;[Chen, Chen; Qin, Xuping; Yang, Li; Yang, Fang; Peng, Hongyan; Hong, Chenliang] Univ South China, Inst Pharm & Pharmacol, Hengyang, Peoples R China.;[Wang, Aiping] Univ South China, Dept Anat, Sch Med, Hengyang, Peoples R China.;[Qin, Xuping] Univ South China, Inst Pharm & Pharmacol, Lab Vasc Biol, 28 Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Qin, Xuping] U;Univ South China, Inst Pharm & Pharmacol, Lab Vasc Biol, 28 Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Warburg effect;Pulmonary arterial smooth muscle cells;Platelet-derived growth factor
摘要：
Background/Aims: The enhanced proliferation of pulmonary arterial smooth muscle cells (PASMCs) is a central pathological component in pulmonary arterial hypertension (PAH). Both the Warburg effect and platelet-derived growth factor (PDGF) are involved in the proliferation of PASMCs. However, the mechanism underlying the crosstalk between the Warburg effect and PDGF during PASMC proliferation is still unknown. We hypothesized that PDGF promotes the Warburg effect via activating the phosphatidylinositol 3-kinase (PI3K) signaling pathway and hypoxia-inducible factor 1-α (HIF-1α) in proliferative PASMCs. Methods: PASMCs were derived from pulmonary arteries of SD rats; cell viability, the presence of metabolites, and metabolic enzyme activities assay were determined by MTT assays, kit assays and western blot analysis, respectively. Results: PDGF promoted PASMC proliferation in a dose-and time-dependent manner, accompanied by an enhanced Warburg effect. Treatment with PDGFR antagonists, Warburg effect inhibitor and PDK1 inhibitor significantly inhibited PI3K signaling activation, HIF-1α expression and PASMC proliferation induced by PDGF, respectively. Furthermore, treatment with PI3K signaling pathway inhibitors remarkably suppressed PDGF-induced PASMC proliferation and the Warburg effect. Conclusion: microplate reader (Biotek, Winooski The Warburg effect plays a critical role in PDGF-induced PASMC proliferation and is mediated by activation of the PI3K signaling pathway and HIF-1α. © 2017 The Author(s). Published by S. Karger AG, Basel.
语种：
英文
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Identification of novel mutations in the HbF repressor gene BCL11A in patients with autism and intelligence disabilities
作者：
Cai, Tao* ;Chen, Xiang;Li, Jinchen;Xiang, Bingwu;Yang, Liu;...
期刊：
American Journal of Hematology ,2017年92(12):E653-E656 ISSN：0361-8609
通讯作者：
Cai, Tao;Liu, P. Paul
作者机构：
[Cai, Tao; Li, Jinchen] Wenzhou Med Univ, Inst Genom Med, Wenzhou, Zhejiang, Peoples R China.;[Cai, Tao] Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD 20892 USA.;[Liu, Yidian; Xiang, Bingwu; Yang, Liu; Chen, Xiang] Wenzhou Med Univ, Phys Med & Rehabil Ctr, Affiliated Hosp 2, Wenzhou, Zhejiang, Peoples R China.;[Liu, Yidian; Xiang, Bingwu; Yang, Liu; Chen, Xiang] Yuying Childrens Hosp, Wenzhou, Zhejiang, Peoples R China.;[Chen, Qiuli] Maternal & Child Hlth Hosp Guangxi Zhuang Autonom, Genet Metab CentraLand Lab, Nanning, Guangxi, Peoples R China.
通讯机构：
[Cai, Tao; Liu, P. Paul] N;Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD 20892 USA.;NHGRI, NIH, Bethesda, MD 20892 USA.
语种：
英文
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鲍曼不动杆菌的耐药率变迁及CRAB耐药机制研究
作者：
杨璐;康秀华;张垂斌;温桂兰
期刊：
南昌大学学报(医学版) ,2015年(6):42-46+54 ISSN：2095-4727
作者机构：
南华大学医学院临床技能中心,湖南 衡阳,421001;南昌大学 第一附属医院呼吸内科,南昌,330006;南昌大学 医学院研究生部,南昌,330006;[张垂斌] 南昌大学;[杨璐] 南华大学
关键词：
鲍曼不动杆菌;耐药;碳青霉烯类;金属酶;碳青霉烯酶;基因型;插入序列基因(IsAba1)
摘要：
目的分析南昌大学第一附属医院鲍曼不动杆菌的耐药率变迁,并研究耐碳青霉烯类鲍曼不动杆菌（carbapenem-resistant acinetobacter baumannii,CRAB）的耐药机制,为临床抗生素的使用提供依据。方法采用VITEK-2-COMPACT全自动微生物鉴定仪进行细菌鉴定及药敏测定,分析2010年及2014年南昌大学第一附属医院鲍曼不动杆菌对临床常用抗生素的耐药分布;同时,选取临床分离的非重复耐碳青霉烯类鲍曼不动杆菌进行耐药机制研究,应用多重聚合酶链反应（PCR）扩增IMP、VIM、OXA-23、0XA-24、0XA-51、OXA-58、IsAba1-blaoxa-23基因,明确该院鲍曼不动杆菌中主要碳青霉烯酶基因表达及其与插入序列IsAba1的关系。结果与2010年相比,2014年检出的鲍曼不动杆菌对大部分临床常用抗生素的耐药率均有上升趋势（除外左氧氟沙星及复方新诺明）;随机选取临床分离的非重复耐碳青霉烯类鲍曼不动杆菌70株和1株对碳青霉烯类敏感的敏感株参照株（carbapenemase sensitive acinetobacter baumannii,CSAB）共71株,用7对特异性引物对70株耐碳青霉烯类鲍曼不动杆菌和1株敏感对照株进行PCR扩增检测显示,70株CRAB及1株敏感菌株中均携带有OXA-51,而仅在CRAB中检出OXA-23、IsAba1-blaOXA-23基因,敏感对照株中未检出该两个基因表达;OXA-58基因在6株CRAB中有表达,均未检出VIM,IMP,OXA-24基因。结论南昌大学第一附属医院鲍曼不动杆菌对临床常用抗生素的耐药性有上升趋势,需进一步加强合理使用抗生素,同时耐碳青霉烯类鲍曼不动杆菌主要耐药基因为bla OXA-23和bla OXA-51,前者可能与bla OXA-23上游存在插入序列IsAba1有关,后者则可能与其上游存在强启动子导致高表达有关,而CRAB耐药与金属酶、OXA-24、OXA-58基因无明显相关性。
语种：
中文
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有创机械通气和常规方法治疗ICU重症心力衰竭临床效果对比研究
作者：
谭位华;卿国忠;陆煜;杨璐
期刊：
现代诊断与治疗 ,2015年(3):491-493 ISSN：1001-8174
作者机构：
南华大学第一附属医院急诊科,湖南 衡阳,421000;南华大学医学院,湖南 衡阳,421001;[杨璐] 南华大学;[卿国忠; 陆煜; 谭位华] 南华大学第一附属医院
关键词：
有创机械通气;常规方法;ICU重症心力衰竭;治疗效果
摘要：
目的探讨有创机械通气和常规方法治疗ICU重症心力衰竭临床治疗效果。方法对来我院诊治的80例患者入院资料进行分析,将其随机分为两组。对照组采用常规方法治疗,试验组采用有创机械通气治疗,比较两组治疗效果。结果试验组治疗30min后30%好转;治疗24h后65%好转,高于对照组（P〈0.05）;试验组治疗72h后NT-pro BNP指标为（1636.3±53.5pg/m L）、LVEF指标为（0.60±0.13）优于对照组（P〈0.05）。结论 ICU重症心力衰竭发病率较高,临床上采用有创机械通气治疗效果理想,能够有效的提高临床治愈率,改善患者肺功能,值得推广使用。
语种：
中文
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miR-23b-3p对肝核因子4的靶基因及效用分析
作者：
童海;雷建军;莫学靓;赵岳;张凯;...
期刊：
湘南学院学报(医学版) ,2015年17(1):1-4 ISSN：1673-498X
作者机构：
南华大学附属附属第一医院功能科,湖南衡阳421001;南华大学心血管疾病研究所动脉硬化学 湖南省重点实验室,湖南衡阳421001;南华大学心血管疾病研究所动脉硬化学 湖南省重点实验室,湖南衡阳,421001;[张凯; 赵岳; 杨璐; 冯祺论; 雷建军; 曾钧发; 房嘉俊; 莫学靓] 南华大学;[童海] 南华大学第一附属医院
关键词：
载脂蛋白(a);靶基因;肝核因子4
摘要：
目的探索miR-23b-3p是否以肝核因子4作为靶基因并下调apo（a）表达,为降高脂蛋白（a）血症提供新的思路。方法用Targetscan在线工具对miR-23b-3p与调控LPA基因的转录因子肝核因子4（HNF4）进行靶基因分析;在JM109细胞中使用荧光素酶报告系统对miR-23b-3p与调控筛选调控LPA基因的转录因子HNF4进行靶基因进行验证实验;用RT-PCR检测Hep G2细胞apo（a）mRNA表达水平。结果 Targetscan生物信息学分析工具表明HNF4G可作为miR-23b-3p的靶基因,荧光素酶报告系统转染miR-23b-3p处理组细胞裂解后荧光强度显著低于对照组,miR-23b-3p的抑制剂可抑制其作用,验证了HNF4G可作为miR-23b-3p的靶基因。转染miR-23b-3p可抑制Hep G2细胞apo（a）mRNA的表达。结论 miR-23b-3p以HNF4为其靶基因,并下调Hep G2细胞apo（a）的表达。
语种：
中文
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Integrated omics study delineates the dynamics of lipid droplets in Rhodococcus opacus PD630
作者：
Chen, Yong;Ding, Yunfeng;Yang, Li;Yu, Jinhai;Liu, Guiming;...
期刊：
Nucleic Acids Research ,2014年42(2):1052-1064 ISSN：0305-1048
通讯作者：
Yu, Jun
作者机构：
[Yang, Fuquan; Huo, Chaoxing; Zhang, Peng; Zhang, Huina; Yang, Li; Ding, Yunfeng; Xie, Zhensheng; Zhang, Shuyan; Xu, Shimeng; Liu, Pingsheng; Chen, Yong; Zhang, Congyan; Wang, Yang; Na, Huimin; Yu, Jinhai] Chinese Acad Sci, Inst Biophys, Natl Lab Macromol, Beijing 100101, Peoples R China.;[Chen, Yong; Zhang, Michael Q.; Li, Yanda] Tsinghua Univ, TNLIST, Ctr Synthet & Syst Biol, Dept Automat,MOE Key Lab Bioinformat, Beijing 100084, Peoples R China.;[Chen, Yong; Zhang, Michael Q.; Li, Yanda] Tsinghua Univ, TNLIST, Ctr Synthet & Syst Biol, Dept Automat,Bioinformat Div, Beijing 100084, Peoples R China.;[Huo, Chaoxing; Zhang, Peng; Yang, Li; Song, Lai; Xu, Shimeng; Zhang, Congyan; Wang, Yang; Zhang, Yue; Na, Huimin; Yu, Jinhai] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Liu, Guiming; Huo, Linhe; Song, Lai; Wang, Xumin; Wang, Guoliang; Yu, Jun; Zhang, Hangxiao; Yu, Dan; Fan, Zhonghua; Zhang, Yue] Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China.
通讯机构：
[Yu, Jun] C;Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China.
摘要：
Rhodococcus opacus strain PD630 (R. opacus PD630), is an oleaginous bacterium, and also is one of few prokaryotic organisms that contain lipid droplets (LDs). LD is an important organelle for lipid storage but also intercellular communication regarding energy metabolism, and yet is a poorly understood cellular organelle. To understand the dynamics of LD using a simple model organism, we conducted a series of comprehensive omics studies of R. opacus PD630 including complete genome, transcriptome and proteome analysis. The genome of R. opacus PD630 encodes 8947 genes that are significantly enriched in the lipid transport, synthesis and metabolic, indicating a super ability of carbon source biosynthesis and catabolism. The comparative transcriptome analysis from three culture conditions revealed the landscape of gene-altered expressions responsible for lipid accumulation. The LD proteomes further identified the proteins that mediate lipid synthesis, storage and other biological functions. Integrating these three omics uncovered 177 proteins that may be involved in lipid metabolism and LD dynamics. A LD structure-like protein LPD06283 was further verified to affect the LD morphology. Our omics studies provide not only a first integrated omics study of prokaryotic LD organelle, but also a systematic platform for facilitating further prokaryotic LD research and biofuel development.
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英文
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腹腔镜肠粘连松解术应用于粘连性肠梗阻并糖尿病患者治疗的临床疗效评价
作者：
杨璐;唐志晗;黄靓;谭位华
期刊：
糖尿病新世界 ,2014年(13):45-45 ISSN：1672-4062
作者机构：
南华大学医学院临床技能中心,湖南衡阳,421000;[唐志晗; 杨璐; 黄靓; 谭位华] 南华大学
关键词：
腹腔镜肠粘连松解术;粘连性肠梗阻;开腹手术
摘要：
目的：探讨腹腔镜肠粘连松解术应用于粘连性肠梗阻治疗的临床疗效，为临床治疗粘连性肠梗阻提供更多的理论参考依据。方法选取2011年3月—2013年4月期间来该院就诊的粘连性肠梗阻患者280例，其中有140例患者并发有糖尿病，将140名患者将其分为对照组和治疗组（根据治疗方式的不同），每组70例，对照组采用传统的开腹手术进行治疗，治疗组采用腹腔镜肠粘连松解术进行治疗，待治疗结束后，对两组患者的疗效进行对比分析。结果在患者下床活动时间、手术后引流例数、手术时间、切口感染、住院时间等方面，治疗组明显优于对照组，两组差异显著具有统计学意义（P<0.05）。结论腹腔镜肠粘连松解术在治疗粘连性肠梗阻方面具有显著疗效，同样也适用于并发糖尿病患者的治疗，该方法创伤小、并发症少，可以减轻患者的痛苦，提高患者的生命质量，值得广大医学工作者在治疗粘连性肠梗阻上大力推广使用。
语种：
中文
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医学专业大学生创新性实验计划项目实践探究
作者：
黄靓;杨璐;吴卓倬;朱梦霞;王汉群
期刊：
现代企业教育 ,2014年(16):157-157 ISSN：1008-1496
作者机构：
南华大学医学院外总教研室;[吴卓倬] 南华大学船山学院;[王汉群; 杨璐; 朱梦霞; 黄靓] 南华大学
关键词：
大学生;创新性实验计划;创新能力
摘要：
大学生创新性实验计划项目是促进学生创新型思维，培养创新性高等人才的重要举措。我校自2008年实施以来，已获得了一些成绩，本文结合自己作为医学专业大学生创新性实验计划项目的指导老师，对大学生创新课题的优点和实践中存在的问题及解决办法进行了深入探究。
语种：
中文
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β-elemene inhibits the proliferation of T24 bladder carcinoma cells through upregulation of the expression of Smad4
作者：
Lu, Xinsheng;Wang, Yi;Luo, Hongmei;Qiu, Weibin;Han, Hu;...
期刊：
MOLECULAR MEDICINE REPORTS ,2013年7(2):513-518 ISSN：1791-2997
通讯作者：
Yang, Luoyan
作者机构：
[Yang, Luoyan; Lu, Xinsheng; Qiu, Weibin; Han, Hu] Cent S Univ, Dept Urol, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China.;[Wang, Yi; Chen, Xian] Cent S Univ, Dept Urol, Affiliated Hosp 2, Changsha 410011, Hunan, Peoples R China.;[Luo, Hongmei] Univ S China, Dept Histol & Embryol, Hengyang 421001, Hunan, Peoples R China.;[Yang, Luoyan] Cent S Univ, Dept Urol, Xiangya Hosp 2, 139 Renmin Zhong Rd, Changsha 410011, Hunan, Peoples R China.
通讯机构：
[Yang, Luoyan] C;Cent S Univ, Dept Urol, Xiangya Hosp 2, 139 Renmin Zhong Rd, Changsha 410011, Hunan, Peoples R China.
关键词：
beta elemene;messenger RNA;Smad4 protein;antineoplastic activity;article;bladder carcinoma;cancer inhibition;cancer survival;carcinoma cell;cell assay;cell proliferation;cell survival;controlled study;gene expression;IC 50;methylthiazolyl tetrazolium assay;minimum inhibitory concentration;reverse transcription polymerase chain reaction;tumor suppressor gene;upregulation;Western blotting;Antineoplastic Agents;Apoptosis;Cell Line, Tumor;Cell Proliferation;Humans;RNA, Messenger;Sesquiterpenes;Smad4 Protein;Up-Regulation;Urinary Bladder Neoplasms
摘要：
β-elemene, a non-cytotoxic antitumor reagent, inhibits the growth, proliferation and DNA synthesis of multiple types of malignant cells, resulting in the apoptosis or suppressed vascularization of tumors. β-elemene is also able to enhance the immunogenicity of the tumor cells and ameliorate systematic cellular immunity in the tumor-bearing body. Moreover, β-elemene has the advantages of high efficiency, safety and low possibility of drug tolerance over other antitumor agents used in antitumor treatment. Therefore, β-elemene has great potential in clinical applications. However, the mechanism of β-elemene antitumor activity is largely unknown. The aim of this study was to investigate whether β-elemene is able to repress the proliferation of T24 bladder carcinoma cells through regulation of the expression of the tumor suppressor gene, Smad4. Results of a methylthiazolyl tetrazolium (MTT) assay indicated that the proliferation of T24 cells was repressed by β-elemene in a time- and concentration dependent manner. The lowest concentration of β-elemene to inhibit cell survival by >50% was determined using IC 50 software. Microscopic observation also demonstrated the potential of β-elemene to induce the apoptosis of cancer cells. Western blot and RT-PCR analyses revealed that the expression of the Smad4 protein and mRNA was upregulated by treatment with β-elemene. Our results revealed that β-elemene was able to upregulate the expression of Smad4 in tumor cells to inhibit the proliferation of these cells. Copyright © 2013 Spandidos Publications Ltd.
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英文
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Repair of avascular meniscal injuries using juvenile meniscal fragments: An in vitro organ culture study
作者：
Dai, Zhu;Li, Kanghua* ;Chen, Zhiwei;Liao, Ying;Yang, Lezhong;...
期刊：
Journal of Orthopaedic Research ,2013年31(10):1514-1519 ISSN：0736-0266
通讯作者：
Li, Kanghua
作者机构：
[Li, Kanghua; Dai, Zhu] Cent S Univ, Xiangya Hosp, Dept Orthoped, Changsha 410078, Hunan, Peoples R China.;[Chen, Zhiwei; Dai, Zhu; Liu, Chunlei; Yang, Lezhong; Liao, Ying] South China Univ, Affiliated Hosp 1, Dept Orthoped, Hengyang 421001, Hunan, Peoples R China.;[Ding, Wenjun] South China Univ, Affiliated Hosp 1, Clin Res Inst, Hengyang 421001, Hunan, Peoples R China.;[Li, Kanghua] Cent S Univ, Xiangya Hosp, Dept Orthoped, 87 Xiangya Rd, Changsha 410078, Hunan, Peoples R China.
通讯机构：
[Li, Kanghua] C;Cent S Univ, Xiangya Hosp, Dept Orthoped, 87 Xiangya Rd, Changsha 410078, Hunan, Peoples R China.
关键词：
collagen type 1;collagen type 2;animal experiment;animal model;article;avascular meniscal injury;biomechanics;bone graft;confocal laser microscopy;confocal microscopy;controlled study;explant;healing;histopathology;immunohistochemistry;in vitro study;juvenile meniscal allograft;knee meniscus rupture;materials testing;meniscal repair;minced meniscal fragment;nonhuman;organ culture;priority journal;tissue repair;in vitro;injury;integrative repair;meniscus;organ culture;Age Factors;Animals;Biomechanics;Collagen Type I;Collagen Type II;Immunohistochemistry;Knee Injuries;Menisci, Tibial;Organ Culture Techniques;Reconstructive Surgical Procedures;Shear Strength;Swine;Transplantation, Homologous;Wound Healing
摘要：
We investigated whether the implantation of juvenile allograft and minced meniscal fragments could improve the healing of avascular meniscal injuries, which cannot heal spontaneously. Concentric cylindrical explants were excised from the inner two-thirds of swine medial menisci. The inner cylinder consisted of a "sandwich" structure, with minced juvenile meniscal fragments, juvenile meniscal columns, minced mature meniscal fragments, or mature meniscal columns implanted in the middle. The explants were cultured in vitro for 2, 4, or 6 weeks. Interfacial meniscal repair was assessed by histology, immunohistochemistry, biomechanical testing, and confocal laser scanning microscopy. Histology and confocal microscopy results revealed that tissue repair and cell accumulation at the interface were best at all time points in the juvenile meniscal fragments group, followed by the juvenile columns, minced mature fragments, and mature columns groups, respectively. At 6 weeks, the implantation of juvenile allograft and minced meniscal fragments increased the shear strength, peak force, and energy to failure in the peripheral interface. Picosirius red/polarized light microscopy and immunohistochemistry results showed concurrent expression of type I and II collagen in the interfacial repair tissue. In conclusion, implantation of juvenile allograft and minced meniscal fragments could increase the healing of avascular meniscal injury in vitro.
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英文
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Diallyl disulfide suppresses proliferation and induces apoptosis in human gastric cancer through Wnt-1 signaling pathway by up-regulation of miR-200b and miR-22
作者：
Tang, Hailin;Kong, Yanan;Guo, Jiaoli;Tang, Yi;Xie, Xinhua;...
期刊：
Cancer Letters ,2013年340(1):72-81 ISSN：0304-3835
通讯作者：
Su, Qi
作者机构：
[Tang, Hailin; Xie, Xinhua; Yang, Lu; Xie, Xiaoming; Kong, Yanan; Guo, Jiaoli] Sun Yat Sen Univ, Ctr Canc, Dept Breast Oncol, Guangzhou 510060, Guangdong, Peoples R China.;[Tang, Hailin; Xie, Xinhua; Yang, Lu; Xie, Xiaoming; Kong, Yanan; Guo, Jiaoli] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol South China, Guangzhou 510060, Guangdong, Peoples R China.;[Tang, Hailin; Su, Qi; Tang, Yi] Univ South China, Canc Res Inst, Hengyang, Hunan, Peoples R China.;[Su, Qi] Sun Yat Sen Univ, Ctr Canc, Dept Breast Oncol, State Key Lab Oncol South China, 651 East Dongfeng Rd, Guangzhou 510060, Guangdong, Peoples R China.
通讯机构：
[Su, Qi] S;Sun Yat Sen Univ, Ctr Canc, Dept Breast Oncol, State Key Lab Oncol South China, 651 East Dongfeng Rd, Guangzhou 510060, Guangdong, Peoples R China.
关键词：
Diallyl disulfide;Gastric cancer;MicroRNA;Wnt-1 signaling pathway
摘要：
The purpose of this study was to identify a mechanism related to miRNA pathway which plays a role in the anti-tumor effects of Diallyl disulfide. Alterations in miRNA expression were observed in Diallyl disulfide-treated MGC-803 cells, including up-regulation of miR-200b and miR-22 expression. Furthermore, Wnt-1 was identified as a target of both miR-200b and miR-22. MiR-200b and miR-22 not only synergistically inhibited gastric cancer growth, but also enhanced the antitumor effect of Diallyl disulfide both in vitro and in vivo. It indicated that miR-200b and miR-22 may serve as potential gene therapy and enhance Diallyl disulfide antitumor effects. © 2013 Elsevier Ireland Ltd.
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英文
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