期刊论文

中文

南昌大学学报(医学版)

2095-4727

2015

6

42-46+54

10.13764/j.cnki.ncdm.2015.06.0010

江西省科技支撑计划（2009BSB11212）；

本校为第一机构

目的分析南昌大学第一附属医院鲍曼不动杆菌的耐药率变迁,并研究耐碳青霉烯类鲍曼不动杆菌（carbapenem-resistant acinetobacter baumannii,CRAB）的耐药机制,为临床抗生素的使用提供依据。方法采用VITEK-2-COMPACT全自动微生物鉴定仪进行细菌鉴定及药敏测定,分析2010年及2014年南昌大学第一附属医院鲍曼不动杆菌对临床常用抗生素的耐药分布;同时,选取临床分离的非重复耐碳青霉烯类鲍曼不动杆菌进行耐药机制研究,应用多重聚合酶链反应（PCR）扩增IMP、VIM、OXA-23、0XA-24、0XA-51、OXA-58、IsAba1-blaoxa-23基因,明确该院鲍曼不动杆菌中主要碳青霉烯酶基因表达及其与插入序列IsAba1的关系。结...

ABSTRACT:Objective To analyze the drug resistance transition of Acinetobacter baumannii (AB)in the First Affiliated Hospital of Nanchang University,and to investigate the drug resist-ance mechanisms of carbapenem-resistant AB for providing a basis for clinical use of antibiotics. Methods Bacteria identification and drug susceptibility determination were performed using VITEK-2-COMPACT automatic microorganism identification machine to analyze the antibiotic resistance distribution of AB in 2010 and 2014.Meanwhile,non-repeated carbapenem-resistant AB (CRAB)was selected to investigate the dr...