CircRNAs: A new perspective of biomarkers in the nervous system
作者：
Ma, Yun;Liu, Yixuan;Jiang, Zhisheng*
期刊：
Biomedicine & Pharmacotherapy ,2020年128:110251 ISSN：0753-3322
通讯作者：
Jiang, Zhisheng
作者机构：
[Liu, Yixuan; Jiang, Zhisheng; Ma, Yun] Univ South China, Hunan Int Sci & Technol Cooperat Base Arterioscle, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis,Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Liu, Yixuan; Ma, Yun] Univ South China, Inst Biochem & Mol Biol, Hengyang 421001, Peoples R China.;[Ma, Yun] Dept Educ, Key Lab Ecol Environm & Crit Human Dis Prevent Hu, Hengyang 421001, Peoples R China.;[Ma, Yun] Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;[Jiang, Zhisheng] Univ South China, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Jiang, Zhisheng] U;Univ South China, Hengyang Med Sch, Hengyang 421001, Peoples R China.
关键词：
Circular RNAs;MicroRNA sponge;Nervous system diseases;Biomarker
摘要：
Circular RNAs (circRNAs) are a class of newly-identified non-coding RNA that lack 5’ (cap) and 3’ (polyadenylation) ends and are linked by a covalent bond to form a closed loop structure. In comparison to linear RNAs, circRNAs are more resistant to exonuclease RNase R-mediated degradation with a much stronger stability due to the absence of 3’ terminals. Consequently, the extraordinary nature of circRNAs enables it to be potentially used as a biomarker and gene targeting. Similarly, circRNAs can play a significant regulatory role in gene expression where it can indirectly regulate the expression of the downstream target genes of microRNAs (miRNAs) by miRNA sponges. The aim of this review is to highlight the function of circRNAs as well as their vital roles in the central nervous system (CNS) regulation and neurological diseases. © 2020 The Authors
语种：
英文
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Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis
作者：
Liu, Yi;Li, Hongguang;Wang, Shuzhi;Yin, Weidong;Wang, Zongbao*
期刊：
Biomedicine & Pharmacotherapy ,2020年129:110321 ISSN：0753-3322
通讯作者：
Wang, Zongbao
作者机构：
[Liu, Yi; Li, Hongguang] Shaoguan Univ, Med Coll, Dept Med Technol, Shaoguan 512026, Guangdong, Peoples R China.;[Wang, Zongbao; Yin, Weidong; Wang, Shuzhi] Univ South China, Sch Pharm, Hengyang 421001, Hunan, Peoples R China.;[Wang, Zongbao; Yin, Weidong; Wang, Shuzhi] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.;[Wang, Zongbao] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Sch Pharm, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wang, Zongbao] U;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Sch Pharm, Hengyang 421001, Hunan, Peoples R China.
关键词：
Lipoprotein lipase activator;Diabetic nephropathy;Swine, Miniature;Nutrient stress;Oxidative stress;Renal fibrosis
摘要：
It is well-recognized that hyperlipidemia and lipid peroxidation contribute to the progression of diabetic nephropathy (DN), which is associated with oxidative stress (OS) and fibrotic lesions. Ibrolipim, a specific lipoprotein lipase activator, has been proved to reduce hyperglycemia and hyperlipidemia, suppress renal lipid deposition, and also protect renal damage. However, the underlying mechanisms of its renoprotective effect are not clearly elaborated. Herein, the present study was to identify whether the putative mechanism of Ibrolipim was related to OS and fibrogenesis in diabetic minipigs fed by high-sucrose and high-fat diet (HSFD) with or without Ibrolipim for 5 months. Compared with the normal control diet, nutrient stress induced by HSFD caused moderate glomerulosclerosis and tubulointerstitial fibrosis, and promoted renal ultrastructural and functional abnormalities. These abnormalities were correlated with renal OS and fibrogenesis characterized by the increased levels of reactive oxygen species (ROS), malondialdehyde, hydroxyproline, collagen type Ⅳ alpha 1 and fibronectin, and decreased contents of reduced glutathione and total antioxidant capacity in kidneys. Ibrolipim significantly ameliorated these abnormalities in HSFD-fed minipigs. In addition, Ibrolipim diminished HSFD-induced nicotinamide-adenine dinucleotide phosphate oxidase-4 activation to reduce ROS production, and enhanced the expression and activity of antioxidant enzymes (i.e. superoxide dismutase 1, catalase and glutathione peroxidase 1) to increase ROS elimination, resulting in obvious suppression of renal OS. Meanwhile, Ibrolipim not only inhibited the upregulation of transforming growth factor-β1 but also partially reversed the downregulation of matrix metalloproteinase 2, and then prevented extracellular matrix (ECM) accumulation. Taken together, Ibrolipim exhibits anti-oxidative and anti-fibrotic effects via modulating the rebalance of renal ROS and ECM metabolism, and ultimately attenuates the progression of nephropathy in diet-induced diabetic minipigs.
语种：
英文
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仪器分析在线教学实践与思考
作者：
刘阳;刘运美;曾要富;彭俊梅;郭玉
期刊：
广州化工 ,2020年(22):220-221 ISSN：1001-9677
作者机构：
南华大学药学院肿瘤微环境响应药物研究湖南省重点实验室
关键词：
在线教学;学习通;腾讯课堂;仪器分析
摘要：
由于新型冠状病毒肺炎疫情的影响,全国高校普遍采用线上教学。仪器分析课程作为药学、药物制剂以及化学类专业学生的一门学科基础必修课程,是运用现代的分析仪器对物质进行定性、定量和结构分析的一门实践性学科。依托学习通与腾讯课堂,从完善教学资源、做好课堂准备、实现在线教学和进行课程反思等方面出发,介绍了地方高校开展仪器分析在线教学的实践经验,期望为相关高校和课程的在线教学提供参考。
语种：
中文
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可注射的壳聚糖水凝胶的制备及应用进展
作者：
刘阳;王毅;王云娟;尹玉利;熊菀伶;...
期刊：
高分子通报 ,2020年2020(5):17-23 ISSN：1003-3726
作者机构：
南华大学药学院,肿瘤微环境响应药物研究湖南省重点实验室,衡阳421001;[熊菀伶; 王毅; 刘阳; 王云娟; 冯晓祎; 尹玉利] 南华大学
关键词：
壳聚糖;水凝胶;可注射
摘要：
可注射的水凝胶由于其独特的性质在生物医学领域中备受关注。壳聚糖是自然界中唯一的一种阳离子多糖,具有含量丰富、价格低廉、生物相容性和生物可降解性良好等优点,经常被用来制备可注射的水凝胶。近年来,随着研究的进一步深入,通过采用各种化学或物理改性和修饰方法、引入各种生物功能分子或采用各种交联方法,具有优异性能的可注射的壳聚糖水凝胶不断涌现,其应用范围不断扩展,在实际应用中发挥了越来越重要的作用。本文主要介绍了常见的化学交联的和物理交联的可注射的壳聚糖水凝胶的制备方法,综述了其在药物载体、基因载体、细胞支架和伤口修复等生物医学领域中的应用进展,对其存在的问题及发展趋势进行了分析和展望,为可注射的壳聚糖水凝胶的进一步发展提供指导和借鉴。
语种：
中文
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优化人才培养方案 促进创新型药学人才培养
作者：
刘阳;雷小勇;郑兴;贺冬秀;刘运美;...
期刊：
卫生职业教育 ,2020年38(23):3-4 ISSN：1671-1246
作者机构：
南华大学药学院/湖南省分子靶标新药研究协同创新中心/肿瘤微环境响应药物研究湖南省重点实验室,湖南衡阳421001;[郑兴; 雷小勇; 刘运美; 刘阳; 喻翠云; 贺冬秀] 南华大学
关键词：
培养方案;本科教育;药学人才
摘要：
培养高水平创新型药学人才是当前经济社会与医药学快速发展的需要,而人才培养方案的修订直接关系到高校创新型人才培养质量。本文以南华大学药学创新实验班为例,结合近年来依托于湖南省分子靶标新药研究协同创新中心的人才培养改革实践,从科学定位培养目标、优化课程体系、完善实践教学环节和改革教育教学方法等方面,对药学人才培养方案的优化进行探讨,以促进高水平创新型药学人才的培养。
语种：
中文
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Novel photosensitizing properties of porphyrin–chrysin derivatives with antitumor activity in vitro
作者：
Liu, Ding;Zhang, Qizhi;Zhang, Lang;Yu, Wenmei;Long, Huizhi;...
期刊：
Journal of Chemical Research ,2020年44(7-8):494-504 ISSN：1747-5198
通讯作者：
Liu, Yunmei
作者机构：
[Liu, Yunmei; Yu, Wenmei; Liu, Ding; Zhang, Qizhi; Long, Huizhi] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Liu, Yunmei; Yu, Wenmei; Liu, Ding; Zhang, Qizhi; Long, Huizhi] Hunan Prov Key Lab Tumor Microenvironm Respons Dr, Hengyang, Peoples R China.;[Zhang, Lang; He, Jun] Univ South China, Inst Chem & Chem Engn, Hengyang, Peoples R China.
通讯机构：
[Liu, Yunmei] U;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
关键词：
porphyrins;chrysins;porphyrin-chrysin derivatives;synthesis;antitumor
摘要：
Photodynamic therapy is a promising cancer treatment with the advantages of low toxicity, high efficiency, and noninvasiveness. In this study, 23 novel porphyrin–chrysin derivatives are synthesized using alkyl carbon chains as bridges. We use human gastric cancer cells (MGC-803) and human cervical cancer cells to evaluate the in vitro antitumor activity of all the porphyrin–chrysin derivatives, with 5-fluorouracil (5-Fu) as a positive control. Several of the prepared compounds showed effective photodynamic killing effects, among which 5-hydroxy-2-phenyl-7-(2-(4-(10,15,20-tris(4-hydroxyphenyl)porphyrin-5-yl)phenoxy)ethoxy)-4H-chromen-4-one shows the highest antiproliferation activity on human cervical cancer cells, with a half maximal inhibitory concentration of 26.51 ± 1.15 µM. Flow cytometry analysis showed that human cervical cancer cell apoptosis might be induced by G1 phase arrest. © The Author(s) 2020.
语种：
英文
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Triple Functional AB(2) Unit-Modulated Facile Preparation of Bioreducible Hyperbranched Copolymers
作者：
Liu, Yuping;Cong, Yong;Ma, Wei;Kang, Guiying;Meng, Chao;...
期刊：
ACS BIOMATERIALS SCIENCE & ENGINEERING ,2020年6(5):2812-2821 ISSN：2373-9878
通讯作者：
Yu, Cuiyun;Wei, Hua
作者机构：
[Liu, Yuping; Wei, Hua; Cong, Yong; Ma, Wei; Meng, Chao; Kang, Guiying; Liu, Fangjun] Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Gansu, Peoples R China.;[Wei, Hua; Yu, Cuiyun; Wei, H] Univ South China, Dept Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.
通讯机构：
[Yu, CY; Wei, H] U;[Wei, Hua] L;Univ South China, Dept Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Gansu, Peoples R China.
关键词：
AB(2) unit;click coupling;RAFT polymerization;hyperbranched polymer template;controlled drug release
摘要：
Facile preparation of hyperbranched polymers (HPs) has been advanced tremendously by the use of either various multifunctional agent-mediated controlled living radical polymerizations or a highly reactive ABx unit-modulated self-stepwise polymerizations. However, it remains, to our knowledge, a significant challenge to prepare HPs with simultaneously precisely controlled degree of branching (DB) and biorelevant signal-triggered degradation property for controlled release applications due to the respective limitations of the aforementioned two strategies. For this purpose, a triple functional AB2 unit, A-SS-B2 chain transfer agent (AB2 CTA), that integrates the merits of both multifunctional agents and highly reactive ABx units was designed and synthesized successfully to include a disulfide bond for reduction-triggered polymer degradation toward promoted intracellular release of encapsulated cargoes, a trithiocarbonate group for a universal reversible addition-fragmentation chain transfer (RAFT) polymerization of any vinyl-based monomer, and three terminal groups consisting of one azide and two alkyne functions for the generation of hyperbranched topology via a self-click coupling-based polymerization. A subsequent self-click polymerization of the resulting AB2 CTA by click coupling in the presence of CuSO4·5H2O and sodium ascorbate (NaVc) generated a hyperbranched polymer template (HPT) with precisely modulated DB and a plurality of CTA units for a universal reversible addition-fragmentation chain transfer (RAFT) polymerization of any vinyl-containing monomer. The HPT was next used as a multimacro-CTA for RAFT polymerization of a typical hydrophilic monomer, oligo(ethylene glycol) monomethyl ether methacrylate (OEGMA), to demonstrate the potential of this HPT for a robust and facile production of bioreducible hyperbranched polymers for controlled release applications. The synthesized HPT-4-POEGMA can form unimolecular micelles with enhanced stability due to the hyperbranched structure, and the size of micelles varied in the range from 82.4 to 140.3 nm by a modulation of the molar feed ratio of monomer to HPT and polymerization time. More importantly, HPT-POEGMA micelles incubated with 10 mM glutathione (GSH) showed reduction-triggered cleavage of the disulfide links and polymer degradation for promoted intracellular doxorubicin (DOX) release and enhanced therapeutic efficiency. Taken together, this triple functional AB2 CTA provided a powerful means for the facile preparation of bioreducible hyperbranched polymers with precisely controlled DB for controlled release applications. Copyright © 2020 American Chemical Society.
语种：
英文
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Synthesis of cyclic graft polymeric prodrugs with heterogeneous grafts of hydrophilic OEG and reducibly conjugated CPT for controlled release
作者：
Meng, Chao;Cao, Yufei;Sun, Lu;Liu, Yuping;Kang, Guiying;...
期刊：
BIOMATERIALS SCIENCE ,2020年8(15):4206-4215 ISSN：2047-4830
通讯作者：
Wei, Hua
作者机构：
[Wei, Hua; Meng, Chao] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Thrget New Dr, Hengyang 421001, Peoples R China.;[Wei, Hua; Meng, Chao] Univ South China, Dept Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Liu, Yuping; Wei, Hua; Peng, Jinlei; Ma, Wei; Deng, Kaicheng; Ma, Liwei; Sun, Lu; Meng, Chao; Cao, Yufei; Kang, Guiying] Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Gansu, Peoples R China.
通讯机构：
[Wei, Hua] U;[Wei, Hua] L;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Thrget New Dr, Hengyang 421001, Peoples R China.;Univ South China, Dept Pharm & Pharmacol, Hengyang 421001, Peoples R China.;Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Gansu, Peoples R China.
摘要：
Fabrication of cyclic graft (cg) copolymer-based polymeric prodrugs by conjugation of drug molecules to cg copolymers via a dynamic covalent bond capable of responding to biorelevant signals integrates simultaneously the merits of cg copolymers and polymeric prodrugs for enhanced stability of nanocarriers and precise modulation of drug release kinetics. To completely eliminate the compromised drug conjugation efficiency due to the steric hindrance of hydrophilic grafts, it will be useful to develop cg polymeric prodrugs with heterogeneous grafts composed of hydrophilic polymers and drug species, respectively. For this purpose, we reported in this study the synthesis of cyclic graft polymeric prodrugs with heterogeneous grafts of hydrophilic oligo (ethylene glycol) (OEG) and reducibly conjugated camptothecin (CPT), cg-poly(oligo(ethylene glycol) monomethyl ether methacrylate)-b-poly((2-hydroxyethyl methacrylate)-disulfide link-camptothecin) (cg-P(OEGMA)-b-P(HEMA-SS-CPT), cg-prodrugs), via an integrated strategy of a previously reported diblock copolymer-based template and post-polymerization intermolecular click conjugation of a reducible CPT prodrug. The micelles self-assembled from cg-prodrugs on one hand had sufficient salt stability due to the branched cg structure, and on the other hand showed a reduction-triggered cleavage of the disulfide link for a promoted CPT release. Most importantly, we uncovered two interesting phenomena of the cg-based polymeric prodrugs as delivery vehicles: (i) the dimensions of both self-assemblies formed by the cg and bottlegraft (bg) polymers depend substantially on the molecular size of the cg and bg polymers likely due to the steric hindrance of the grafted structures of the cg and bg molecules and relatively low aggregation number of the self-assembled structures, and (ii) cg-prodrug-based micelles exhibited greater in vitro cytotoxicity against cancer cells despite the lower drug loading content (DLC) than the bg-based analogues, which results primarily from the faster reduction-triggered degradation and drug release as well as the greater cellular uptake efficiency of the former micelle prodrugs. Taken together, the developed cg-prodrugs provide great potential for chemotherapy, and the aforementioned interesting results will definitely inspire more upcoming studies on the future design and development of novel cg polymers for biomedical applications. This journal is © The Royal Society of Chemistry.
语种：
英文
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Association of PD-L1 gene rs4143815 C>G polymorphism and human cancer susceptibility: A systematic review and meta-analysis
作者：
Zou, Ju;Wu, Daichao* ;Li, Tao;Wang, Xianwen;Liu, Yan;...
期刊：
PATHOLOGY RESEARCH AND PRACTICE ,2019年215(2):229-234 ISSN：0344-0338
通讯作者：
Wu, Daichao;Tan, Sijie
作者机构：
[Liu, Yan; Zou, Ju] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hunan Prov Key Lab Special Pathogens Prevent & Co, Dept Parasitol,Hengyang Med Coll, Hengyang 421001, Hunan, Peoples R China.;[Tan, Sijie; Wu, Daichao] Univ South China, Hengyang Med Coll, Dept Histol & Embryol, Inst Clin Anat & Reprod Med, Hengyang 421001, Hunan, Peoples R China.;[Li, Tao; Wang, Xianwen] Univ South China, Hengyang Med Coll, Grade 2015 Clin Med, Hengyang 421001, Hunan, Peoples R China.;[Wu, Daichao; Tan, SJ] Univ South China, Hengyang Med Coll, Inst Clin Anat & Reprod Med, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wu, DC; Tan, SJ] U;Univ South China, Hengyang Med Coll, Inst Clin Anat & Reprod Med, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
biological marker;programmed death 1 ligand 1;CD274 protein, human;programmed death 1 ligand 1;bladder cancer;cancer risk;cancer susceptibility;colorectal cancer;disease marker;esophageal squamous cell carcinoma;genetic association;genetic linkage;genetic polymorphism;genetic variability;human;liver cell carcinoma;meta analysis;non small cell lung cancer;ovary cancer;Review;single nucleotide polymorphism;stomach cancer;systematic review;genetic predisposition;genetics;genotype;neoplasm;odds ratio;B7-H1 Antigen;Genetic Predisposition to Disease;Genotype;Humans;Neoplasms;Odds Ratio;Polymorphism, Single Nucleotide
摘要：
Programmed death ligand 1(PD-L1) mediated immune escape play important roles in the development of cancer. The gene polymorphism of PD-L1, in particular rs4143815 C > G, has been associated with the cancer risks, but with conflicting results. Therefore, this meta-analysis was aimed to assess the association between rs4143815 C > G and cancer susceptibility. A systematic literature search was performed to select the studies and the pooled odds ratio (OR) with 95% confidence interval (CI) was used to evaluate the strength of association. Eleven eligible studies containing 3711 cases and 3704 controls were enrolled in the meta-analysis. The results suggested that there is a strong association between rs4143815 C > G and the cancer risks (G vs. C: OR = 1.386, 95% CI: 1.132–1.696, p = 0.002; GG vs. CG + CC: OR = 1.843 95% CI: 1.300–2.613, p = 0.002; GG + CG vs. CC: OR = 1.280, 95% CI: 1.040–1.576, p = 0.020). Subgroup analysis based on cancer type suggested that PD-L1 rs4143815 C > G might increase the susceptibility to gastric cancer (G vs. C: OR = 1.842, 95% CI: 1.403–2.418, p < 0.001) and bladder cancer (G vs. C: OR = 2.015, 95% CI: 1.556–2.608, p < 0.001), and genotype GG carriers of PD-L1 rs4143815 C > G might have higher risks of HCC (GG vs. CG + CC: OR = 2.226 95% CI: 1.562–3.172, p < 0.001). PD-L1 rs4143815 C > G might confer an increased cancer risk, indicating this SNP may contribute to the pathogenesis of cancer and might be used as a potential biomarker to predict the susceptibility to cancer. © 2018 Elsevier GmbH
语种：
英文
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聚N-异丙基丙烯酰胺温敏水凝胶的研究与应用进展
作者：
刘阳;尹玉利;贺艳
期刊：
高分子通报 ,2019年2019(7):13-19 ISSN：1003-3726
作者机构：
南华大学药学院,湖南省分子靶标新药研究协同创新中心,衡阳421001;南华大学图书馆,衡阳,421001;[贺艳; 刘阳; 尹玉利] 南华大学
关键词：
聚N-异丙基丙烯酰胺;水凝胶;温敏性
摘要：
温敏水凝胶能够对温度的改变进行响应,在实际应用中备受关注。其中,聚N-异丙基丙烯酰胺（PNIPAM）由于温敏性良好、制备简单、易于修饰等优点,是研究和应用最为广泛的一种温敏水凝胶。近年来,随着研究的进一步深入,特别是化学改性和修饰技术的进步,各种具有优异响应性能的新型PNIPAM温敏水凝胶不断出现,其应用的深度和广度不断扩展和更新,更为贴近实际需求,在生物医药、化学化工等领域前景广阔。本文介绍了PNIPAM温敏水凝胶的温敏性原理及主要的制备方法,重点综述了其在药物释放、细胞支架、传感分析和物质分离等方面的应用现状和发展趋势,对其存在的问题及发展前景进行了分析和展望,为PNIPAM温敏水凝胶的进一步发展提供借鉴和参考。
语种：
中文
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医药类专业文献检索课程教学改革创新
作者：
贺艳;刘阳
期刊：
广东化工 ,2019年46(16):204,209 ISSN：1007-1865
作者机构：
南华大学图书馆,湖南衡阳,421001;南华大学药学院,湖南衡阳,421001;[贺艳; 刘阳] 南华大学
关键词：
文献检索;教学改革创新;医药类
摘要：
文献检索是一门综合介绍各类信息文献资源的查找与运用的课程,是提高医药类专业学生的信息素养的主要课程。为提高课程的教学效果,促进医药类创新人才的培养,在分析医药类专业文献检索课程教学现状的基础上,从教学内容、教学方法、师资队伍等方面对该课程的教学改革创新进行了探讨。
语种：
中文
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组织工程支架材料研究进展
作者：
刘阳;莫春香;贺艳;李蓉;曾晨星;...
期刊：
化工新型材料 ,2019年47(12):37-40 ISSN：1006-3536
作者机构：
南华大学药学与生物科学学院, 湖南省分子靶标新药研究协同创新中心, 衡阳, 421001;南华大学图书馆, 衡阳, 421001;[刘阳; 莫春香; 李蓉; 曾晨星; 罗成旺; 史亚娟] 南华大学药学与生物科学学院, 湖南省分子靶标新药研究协同创新中心, 衡阳, 421001;[贺艳] 南华大学图书馆, 衡阳, 421001
关键词：
支架材料;组织工程;天然材料;人工合成材料;复合材料
摘要：
组织工程在组织或器官的修复和再生中发挥着越来越重要的作用。其中支架材料是组织工程的重要组成部分,能够为种子细胞的粘附、生长、增殖和分化等提供临时的机械支撑以及必要的生长环境,因而显得尤为重要。支架材料按照来源可以分为天然材料、人工合成材料和复合材料。从材料学的角度,介绍了骨、神经、牙齿及血管等组织工程领域中常见的支架材料的研究与应用进展,并对支架材料的发展前景进行了展望。
语种：
中文
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Ghrelin attenuates myocardial fibrosis after acute myocardial infarction via inhibiting endothelial-to mesenchymal transition in rat model
作者：
Chen, Hainan;Liu, Yijian;Gui, Qingjun;Zhu, Xiao;Zeng, Lin;...
期刊：
Peptides ,2019年111(1):118-126 ISSN：0196-9781
通讯作者：
He, Jin;Yin, Kai
作者机构：
[Gao, Ling; Chen, Hainan; Yin, Kai; Feng, Juling; Jackson, Ampadu O.; Li, Yi; Gui, Qingjun; Qing, Jina; Zhu, Xiao] Univ South China, Med Sch, Res Lab Clin & Translat Med, Hengyang 421001, Peoples R China.;[Chen, Hainan; Yin, Kai; Zhu, Xiao] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Liu, Yijian] Third Hosp Changsha, Changsha 410000, Hunan, Peoples R China.;[Zeng, Lin] Univ South China, Affiliated Hosp 1, Dept Neurol, Hengyang 421001, Peoples R China.;[Meng, Jun; He, Jin] Univ South China, Affiliated Hosp 1, Funct Dept, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[He, Jin; Yin, Kai] U;Univ South China, Affiliated Hosp 1, Funct Dept, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Res Lab Clin & Translat Med, Hengyang 421001, Peoples R China.
关键词：
collagen;ghrelin;ghrelin receptor;hydroxymethylglutaryl coenzyme A reductase kinase;Smad2 protein;Smad3 protein;Smad7 protein;sodium chloride;transforming growth factor beta1;ghrelin;ghrelin receptor;acute heart infarction;animal experiment;Article;attenuation;body weight;CAEC cell line;controlled study;coronary artery ligation;drug blood level;drug mechanism;epithelial mesenchymal transition;heart muscle fibrosis;heart weight;human;human cell;human cell culture;in vitro study;in vivo study;left anterior descending coronary artery;male;nonhuman;priority journal;protein expression;protein phosphorylation;rat;serum;signal transduction;animal;blood;cardiomyopathy;cell culture;echocardiography;enzyme linked immunosorbent assay;epithelial mesenchymal transition;fibrosis;fluorescent antibody technique;heart infarction;metabolism;real time polymerase chain reaction;Sprague Dawley rat;Western blotting;Animals;Blotting, Western;Cardiomyopathies;Cells, Cultured;Echocardiography;Enzyme-Linked Immunosorbent Assay;Epithelial-Mesenchymal Transition;Fibrosis;Fluorescent Antibody Technique;Ghrelin;Humans;Male;Myocardial Infarction;Rats;Rats, Sprague-Dawley;Real-Time Polymerase Chain Reaction;Receptors, Ghrelin
摘要：
Ghrelin, a peptide hormone produced in the gastrointestinal tract, has recently been found to be associated with the onset of myocardial fibrosis (MF). The exact mechanism, however, remains elusive. This study sought to identify the function and mechanism of ghrelin on MF after acute myocardial infarction (AMI). AMI was established in Spraque-Dawley rats by ligation of the left anterior descending (LAD). Ghrelin or saline was intraperitoneally injected two times per day for 8 weeks after ligation. The weight of heart (mg) and the weight ratio of heart to body (mg/g) as well as the fibrotic area were increased, while serum level of ghrelin was decreased after AMI. Ghrelin significantly ameliorated MF and decreased deposition of collagens in perivascular fibrosis area. In addition, ghrelin inhibited Endothelial-to-mesenchymal transition (EndMT), a crucial process for MF, in perivascular fibrosis area and TGF-β1-induced human coronary artery endothelial cells (HCAECs). Mechanistically, ghrelin persistently decreased the phosphorylation of Smad2/3 and enhanced the expression of Smad7 and p-AMPK in vivo and in vitro. After the abolition of Smad7, GHSR-1a and AMPK pathway, the effect of ghrelin on EndMT was significantly inhibited. In conclusion, these results presented a novel finding that ghrelin attenuated MF after AMI via regulation EndMT in a GHSR-1a/AMPK/Smad7- dependent manner. © 2018 Elsevier Inc.
语种：
英文
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The Relationship between Pharmacological Properties and Structure- Activity of Chrysin Derivatives
作者：
Li, Yang;Li, Yan-peng;He, Jun* ;Liu, Ding;Zhang, Qi-zhi;...
期刊：
MINI-REVIEWS IN MEDICINAL CHEMISTRY ,2019年19(7):555-568 ISSN：1389-5575
通讯作者：
He, Jun;Liu, Yunmei
作者机构：
[Liu, Yunmei; Li, Kang; Guo, Yu; Liu, Ding; Li, Yan-peng; Tang, Guo-Tao; Zhang, Qi-zhi; Li, Yang; Zheng, Xing] Univ South China, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;[He, Jun] Univ South China, Inst Chem & Chem Engn, Hengyang 421001, Peoples R China.;[He, J; Liu, YM] Univ South China, Inst Pharm & Pharmacol, 28 Changshcng West Rd, Hengyang 421001, Peoples R China.
通讯机构：
[He, J; Liu, YM] U;Univ South China, Inst Pharm & Pharmacol, 28 Changshcng West Rd, Hengyang 421001, Peoples R China.
关键词：
5 [(4 fluorobenzyl)oxy] 7 hydroxy 2 phenyl 4h chromen 4 one;7 o (ethoxycarbonyl)alkyl chrysin;7 o (ethoxycarbonyl)alkylamino chrysin;antiinfective agent;antivirus agent;chrysin;chrysin derivative;selenium;unclassified drug;antineoplastic agent;biological product;chrysin;flavonoid;antidiabetic activity;antineoplastic activity;antiviral activity;apoptosis;Article;cancer cell destruction;chemical modification;clinical outcome;drug mechanism;drug research;drug screening;drug structure;Enterovirus A71;G2 phase cell cycle checkpoint;gene therapy;human;inflammation;M phase cell cycle checkpoint;nonhuman;structure activity relation;animal;chemistry;drug development;drug effect;neoplasm;procedures;structure activity relation;Animals;Antineoplastic Agents;Apoptosis;Biological Products;Drug Discovery;Flavonoids;Humans;Neoplasms;Structure-Activity Relationship
摘要：
Chrysin is a natural product of a flavonoid compound. Chemically, chrysin consists of two phenyl rings (A and B) and a heterocyclic ring (C). Biologically, chrysin exerts many different physiological activities. In recent years, with the in-depth development for more active drugs, the synthesis and biological activities of chrysin derivatives have been well studied. Besides, structure-activity relationship of chrysin revealed that the chemical construction meets the critical chemical structural necessities of flavonoids for numerous pharmacological activities. It is generally believed that modified chrysin could be more potent than unmodified chrysin. Different modification in the rings of chrysin could possess various degrees of biological activities. This review aims to summarize the mechanism for the activities of chrysin and its derivatives in different rings. We also explored the relationship between biological function and structure-activity of substituted chrysin derivatives with different functional groups. The influence of chrysin derivatives on the proliferation and apoptosis of cancer cells is also investigated. Development of novel drugs based on the biological functions of chrysin could better improve clinical outcomes of affected population, especially for tumor patients and diabetic patients. © 2019 Bentham Science Publishers.
语种：
英文
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The association between anti-tumor potency and structure-activity of protein-kinases inhibitors based on quinazoline molecular skeleton
作者：
Li, Yang;Xiao, Jie;Zhang, Qizhi;Yu, Wenmei;Liu, Mengqin;...
期刊：
Bioorganic & Medicinal Chemistry ,2019年27(3):568-577 ISSN：0968-0896
通讯作者：
Liu, Yunmei;He, Jun
作者机构：
[Xiao, Jie; Liu, Yunmei; Yu, Wenmei; Guo, Yu; Zhang, Qizhi; Li, Yang] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[He, Jun] Univ South China, Inst Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Liu, Mengqin] Hengyang Normal Univ, Inst Chem & Mat Sci, Hengyang 421001, Peoples R China.
通讯机构：
[Liu, Yunmei; He, Jun] U;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;Univ South China, Inst Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Anti-tumor;Drug target;Inhibitors of protein kinases;Quinazoline derivatives;Structure-activity relationship
摘要：
Quinazoline was originally utilized as an anti-tumor treatment, and its various derivatives can be directly extracted from plants. In recent years, protein kinases (PK) have been well recognized in the development of tumor drugs. Functionally, PK serves a vital role in the apoptosis, proliferation, differentiation, migration and cell cycle of tumor cells. Due to its good physicochemical properties, quinazoline skeleton, a superior type of PK inhibitor, has been extensively used in anti-tumor drug design. An increasing number of studies on quinazoline synthesis have been reported and used by different groups to effectively develop novel derivatives. Thus, several studies have been approved for the use of quinazoline derivatives as inhibitors of other kinases, including Src and histone deacetylase. The aim of the present review was to summarize the mechanism of quinazoline compounds as PK inhibitors, their biological structure-activity relationship such as the substituted quinazoline compounds with different functional groups in the apoptotic process, and their effect on the proliferation of tumor cells. The development of novel agents based on the antitumor functions of quinazoline molecular compounds may improve the clinical outcomes of the affected population, particularly in patients with cancer.
语种：
英文
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有机药物波谱解析教学改革探讨
作者：
刘阳;杨寒幸;刘运美;彭俊梅;贺冬秀
期刊：
药学教育 ,2018年34(3):44-46 ISSN：1007-3531
作者机构：
南华大学药学与生物科学学院 衡阳 421001;[彭俊梅; 刘运美; 刘阳; 杨寒幸; 贺冬秀] 南华大学
关键词：
有机药物波谱解析;理论教学;实验教学;信息化教学;自主教学
摘要：
有机药物波谱解析是药学、制药工程及化学类专业学生的一门重要的专业基础课程,具有内容繁杂、抽象难懂、应用性强等特点。以南华大学药学专业为例,针对课程教学中存在的问题,结合教学经验和学习体会,对有机药物波谱解析精品课程的理论教学、实验教学、信息化教学以及学生自主教学进行初步探讨。
语种：
中文
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药学研究生药用高分子材料学教学改革初探
作者：
刘阳;贺艳;喻翠云;贺冬秀;雷小勇;...
期刊：
广州化工 ,2018年46(17):128-129 ISSN：1001-9677
作者机构：
南华大学药学与生物科学学院,湖南省分子靶标新药研究协同创新中心,湖南衡阳421001;[贺艳; 郑兴; 雷小勇; 刘阳; 喻翠云; 贺冬秀; 陈临溪] 南华大学
关键词：
药用高分子材料学;教学改革;研究生
摘要：
药用高分子材料学是药学类专业研究生的一门重要的基础课程,在创新性药学高级人才的培养中发挥了重要作用。为了提高课程的教学质量,培养研究生的创新能力,以南华大学药学研究生为例,在对该课程的教学现状进行分析的基础上,从教学内容的选择、教学方法的采用、自主学习的加强等方面,对该课程的教学改革进行了初步探讨。
语种：
中文
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Synthesis and evaluation of antitumour activity in vitro and in vivo of chrysin salicylate derivatives
作者：
Deng, Xiangping;Zhao, Zihao;Xiong, Shujuan;Xiong, Runde;Liu, Juan;...
期刊：
Natural Product Research ,2018年32(18):2178-2186 ISSN：1478-6419
通讯作者：
Liu, Yunmei;Tang, Guotao
作者机构：
[Wang, Zhe; Liu, Yunmei; Zou, Liu; Liu, Juan; Tang, Guotao; Zheng, Xing; Deng, Xiangping; Xie, Zhizhong; Liu, YM; Tang, GT; Lei, Xiaoyong; Xiong, Shujuan; Zhao, Zihao; Xiong, Runde; Cao, Xuan] Univ South China, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang, Peoples R China.;[Zhao, Zihao] Xiangtan Cent Hosp, Pharm Dept, Xiangtan, Peoples R China.;[Chen, Yanming] Mu Dan Jiang You Bo Pharmacert Co Ltd, Mudanjiang, Peoples R China.
通讯机构：
[Liu, YM; Tang, GT] U;Univ South China, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang, Peoples R China.
关键词：
antineoplastic agent;chrysin;chrysin salicylate derivative;fluorouracil;salicylic acid derivative;unclassified drug;antineoplastic agent;chrysin;flavonoid;salicylic acid derivative;animal experiment;animal model;animal tissue;antineoplastic activity;Article;cancer cell line;cell cycle G1 phase;cell cycle S phase;controlled study;drug efficacy;drug megadose;drug potency;drug screening;drug structure;drug synthesis;flow cytometry;Hep-G2 cell line;human;human cell;IC50;in vitro study;in vivo study;MCF-7 cell line;MFC cell line;MGC-803 cell line;mouse;nonhuman;stomach carcinoma;animal;cell proliferation;drug effect;structure activity relation;synthesis;tumor cell line;Animals;Antineoplastic Agents;Cell Line, Tumor;Cell Proliferation;Drug Screening Assays, Antitumor;Flavonoids;Hep G2 Cells;Humans;MCF-7 Cells;Mice;Salicylates;Structure-Activity Relationship
摘要：
A series of chrysin salicylate derivatives as potential antitumour agents were synthesised and evaluated their antitumour activities in vitro and in vivo. Most of the compounds exhibited moderate to good activities against MCF-7 cells, HepG2 cells, MGC-803cells and MFC cells. Among them, compound 3f showed the most potent activity against MGC-803 cells and MFC cells with IC50 values of 23.83±3.68 and 27.34±5.21μM, respectively. The flow cytometry assay reconfirmed that compound 3f promoted the occurrence of tumour cells’ G1/S block under the inhibiting effect of compound 3f. Compound 3f possessed higher antitumour efficacy in tumour-bearing mice, compared with the positive control 5-Fu and the blank control saline. © 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group.
语种：
英文
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Synthesis of novel genistein amino acid derivatives and investigation on their interactions with bovine serum albumin by spectroscopy and molecular docking
作者：
Long, Xiaokang;Zeng, Yao-Fu;Liu, Yunmei;Liu, Ying;Li, Tangluo;...
期刊：
RSC Advances ,2018年8(54):31201-31212 ISSN：2046-2069
通讯作者：
Guo, Yu
作者机构：
[Liu, Yunmei; Liu, Ying; Li, Tangluo; Guo, Yu; Long, Xiaokang; Liao, Lanqing; Zeng, Yao-Fu] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
通讯机构：
[Guo, Yu] U;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
摘要：
Genistein amino acid derivatives 4a–4d were synthesized and evaluated for their cytotoxic activities against MCF-7, Hela, MGC-803 and HCT-116 cell lines by MTT assays in vitro. The results revealed that compounds 4a–4d showed better activity than the parent compound genistein. Particularly, compound 4b displayed the most significant anticancer activity against MGC-803 with an IC50 value of 12.08 μM. In addition, the mechanisms of interaction between genistein, compounds 4a–4d and BSA were investigated via multi-spectroscopic techniques such as ultraviolet (UV) spectroscopy, fluorescence, circular dichroism (CD), and molecular docking under physiological conditions. The results suggested that endogenous fluorescence of BSA could be quenched by genistein and compounds 4a–4d via forming BSA-compound complex, which meant a static quenching mechanism was involved. The negative values of enthalpy (ΔH) and entropy (ΔS) indicated that interactions between BSA and the ligands were spontaneous, and hydrogen bonding and van der Waals interactions were involved in the BSA-compound complexion formation. The UV, synchronous and 3D fluorescence results revealed that the micro-environment of tryptophan and conformation of BSA were changed after binding to ligands. CD analysis demonstrated the variation in the secondary structure and that the α-helix content of BSA decreased. Eventually, molecular docking was executed to forecast the binding forces and binding sites between BSA and compounds 4a–4d.
语种：
英文
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Design, synthesis and biological evaluation of chrysin benzimidazole derivatives as potential anticancer agents
作者：
Wang, Zhe;Deng, Xiangping;Xiong, Shujuan;Xiong, Runde;Liu, Juan;...
期刊：
Natural Product Research ,2018年32(24):2900-2909 ISSN：1478-6419
通讯作者：
Zheng, Xing;Tang, Guotao
作者机构：
[Wang, Zhe; Liu, Yunmei; Zou, Liu; Liu, Juan; Zheng, X; Tang, GT; Tang, Guotao; Zheng, Xing; Deng, Xiangping; Xie, Zhizhong; Lei, Xiaoyong; Xiong, Shujuan; Xiong, Runde; Cao, Xuan] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pharm & Pharmacol, Hengyang, Peoples R China.;[Chen, Yanming] Mu Dan Jiang You Bo Pharmacert Co Ltd, Mudanjiang, Peoples R China.
通讯机构：
[Zheng, X; Tang, GT] U;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pharm & Pharmacol, Hengyang, Peoples R China.
关键词：
antineoplastic agent;benzimidazole derivative;chrysin;compound 10;compound 11;compound 12;compound 13;compound 14;compound 15;compound 16;compound 17;compound 18;compound 19;compound 21;compound 22;compound 23;compound 3;compound 4;compound 5;compound 6;compound 7;compound 8;compound 9;fluorouracil;unclassified drug;antineoplastic agent;benzimidazole derivative;chrysin;flavonoid;animal experiment;animal model;antineoplastic activity;antiproliferative activity;apoptosis;Article;breast cancer;cancer inhibition;cell cycle arrest;cell cycle G0 phase;cell cycle G1 phase;controlled study;dose response;drug design;drug megadose;drug potency;drug screening;drug synthesis;Hep-G2 cell line;IC50;in vitro study;in vivo study;male;MCF-7 cell line;mouse;nonhuman;animal;cell cycle;cell proliferation;chemistry;drug effect;drug screening;human;synthesis;tumor cell line;xenograft;Animals;Antineoplastic Agents;Apoptosis;Benzimidazoles;Cell Cycle;Cell Line, Tumor;Cell Proliferation;Drug Design;Drug Screening Assays, Antitumor;Flavonoids;Heterografts;Humans;Mice
摘要：
A series of chrysin benzimidazole derivatives were synthesised and evaluated for their anticancer activity in the search for potential anticancer agents. Among them, compound 18 displayed the most potent anti-proliferative activity against MFC cells with IC 50 values of 25.72±3.95μM. The flow cytometry results displayed that compound 18 induced apoptosis of MFC cells in a dose-dependent manner and caused the cell cycle to be arrested in the G0/G1 phase. Furthermore, the preliminary anticancer activity in vivo was also studied in tumour-bearing mice, and the compound 18 exerted good inhibition effect on tumour growth. These results suggested that compound 18 had good anticancer activity, which could be a potential anticancer agent after further optimisation and evaluation. © 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group.
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英文
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