作者机构:
[Song, Fengmei; Tang, Xiaomin; Cao, Yi; Zhao, Weichao] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Huang, Chaobo] Nanjing Forestry Univ NFU, Coll Chem Engn, Nanjing 210037, Peoples R China.;[Dai, Xuyan] Hunan Agr Univ, Econ Coll, Changsha 410128, Peoples R China.
通讯机构:
[Yi Cao] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China
摘要:
Ferroptosis has been linked to the pathogenesis of hepatic injury induced by ischemia/reperfusion (I/R). However, the mechanistic basis remains unclear. In this study, by using a mouse model of hepatic I/R injury, it is observed that glutathione (GSH) and cysteine depletion are associated with deficiency of the reducing power of nicotinamide adenine dinucleotide phosphate (NADPH). Genes involved in maintaining NADPH homeostasis are screened, and it is identified that I/R-induced hepatic ferroptosis is significantly associated with reduced expression and activity of NADP(+)-dependent malic enzyme 1 (Me1). Mice with hepatocyte-specific Me1 gene deletion exhibit aggravated ferroptosis and liver injury under I/R treatment; while supplementation with L-malate, the substrate of ME1, restores NADPH and GSH levels and eventually inhibits I/R-induced hepatic ferroptosis and injury. A mechanistic study further reveals that downregulation of hepatic Me1 expression is largely mediated by the phosphatase and tensin homologue (PTEN)-dependent suppression of the mechanistic target of rapamycin/sterol regulatory element-binding protein 1 (mTOR/SREBP1) signaling pathway in hepatic I/R model. Finally, PTEN inhibitor, mTOR activator, or SREBP1 over-expression all increase hepatic NADPH, block ferroptosis, and protect liver against I/R injury. Taken together, the findings suggest that targeting ME1 may provide new therapeutic opportunities for I/R injury and other ferroptosis-related hepatic conditions.
作者机构:
[Gao Xiao-yu; Qian Duo; Hu Cong-cong; Yang Sheng-yuan; Liu Zhi-yuan; Liu Bin; Su Wen-en; Yi Yu-xin] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构:
[Yang, SY ] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
关键词:
大豆蛋白金纳米簇;2,6-吡啶二羧酸;生物标志物;炭疽杆菌;荧光探针
摘要:
炭疽杆菌是高致病性病原微生物, 引起的炭疽病在我国属于乙类传染病, 因此建立操作简便、 灵敏准确的炭疽杆菌检测方法对预防和控制炭疽传播, 维护公共卫生安全至关重要。 该研究创新性地提出以绿色材料大豆蛋白为保护剂和还原剂, 采用微波加热法合成了一种具有强烈红色荧光发射的大豆蛋白金纳米簇(SPI-AuNCs)。 采用TEM、 UV-Vis、 FL、 XPS、 FTIR等方法表征了SPI-AuNCs的成功合成和部分特殊性能。 结果表明SPI-AuNCs呈球形, 粒径大小在1.8~3.2 nm范围内, 平均粒径为2.65 nm, 在500~550 nm范围内未出现表面等离子体共振峰; SPI-AuNCs的最大激发波长为370 nm, 最大发射波长为680 nm。 SPI-AuNCs的表面官能团主要有—NH、 —COOH、 —OH、 —SH等官能团, 元素的组成主要包括C、 N、 O、 S、 Au元素。 根据Cu2+与SPI-AuNCs表面基团通过配位作用形成非荧光复合物, 使荧光猝灭; 而2,6-吡啶二羧酸(DPA)与Cu2+具有更高的亲和力, 可将Cu2+从SPI-AuNCs表面竞争下来, 使荧光恢复, 据此建立了一种荧光“关-开”策略检测DPA的新方法。 在最佳实验条件下, 荧光恢复率(ΔF/F1)与DPA的浓度在1.15~70.0 μmol·L-1范围内呈良好的线性关系, 线性方程为ΔF/F1=0.011c+0.131, 相关系数r=0.991, 方法检出限为0.34 μmol·L-1。 同时, 通过加标回收实验研究了湖水和牛奶样品中DPA, 得到加标回收率在97.3%~103.6%, 表明了该方法在环境和食品样本DPA的检测中具有很大的应用潜力, 可以为环境监测和食品安全提供方法学支持。 Bacillus anthracis is a highly pathogenic microorganism. Anthrax is an infectious disease caused by Bacillus anthracis, classified as Class B of our country’s statutory reporting infectious diseases. Therefore, the establishment of simple operation, rapid and sensitive detection methods for Bacillus anthracis is vital for preventing and contralling the spread of anthrax and maintaining public health security. This study innovatively proposed to synthesize soy protein gold nanoclusters (SPI-AuNCs) with strong red fluorescence emission by microwave heating using the green material soy protein as a protective agent and reducing agent.TEM, XPS, FTIR, FL, and UV-Vis characterizations were used to verify successful synthesis of SPI-AuNCs. SPI-AuNCs are spherical, which the particle size is in the range of 1.8~3.2 nm, an average diameter of 2.65 nm, and no surface plasma resonance absorption from 500 to 550 nm. The maximum excitation wavelength of SPI-AuNCs is 370 nm, and the maximum emission wavelength appears at 680 nm. The surface functional groups of SPI-AuNCs mainly include —NH, —COOH, —OH, —SH, and the element composition includes C, N, O, S, Au elements. The fluorescence of SPI-AuNCs could be quenched by the coordination between Cu2+ and surface groups of SPI-AuNCs, while DPA has a stronger chelation effect with Cu2+, which could compete for Cu2+ from the surface of SPI-AuNCs and restore the fluorescence of SPI-AuNCs. Accordingly, a new method for DPA detection based on the fluorescent “off-on” strategy was established. Under the optimal experimental conditions, the fluorescence recovery efficiency (ΔF/F1) performs a good linear relationship with DPA concentration in the range of 1.15~70.0 μmol·L-1. The linear regression equation is ΔF/F1=0.011c+0.131 with high correlation coefficient (r=0.991), and the detection limit is 0.34 μmol·L-1. In addition, the spiking experiments of the DPA in lake water and milk samples were performed. The spiked recoveries were 97.3%~103.6%, indicating that this method has great application potential in DPA detection for environmental and food samples, and can provide methodological guidance for environmental monitoring and food safety.
通讯机构:
[Jianming Pan] I;[Lizhang Xu; Xiangheng Niu] S;School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China
作者机构:
[Liu, Jie; Yang, Shengyuan; Du, Wenfang; Tan, Yan; Li, Hong; Luo, Jie; Feng, Qianqian; Deng, Chenyi; Xiao, Fubing] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci,Hunan Key Lab Typ Environm, Hengyang 421001, Peoples R China.;[Du, Wenfang; Wu, Zhaoyang; Xiao, Fubing] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;[Du, Wenfang] Binzhou Univ, Coll Biol & Environm Engn, Binzhou 256600, Peoples R China.
通讯机构:
[Fubing Xiao] H;Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
作者机构:
[Long, Xizi] Univ South China, Sch Key Lab Typ Environm Pollut & Hlth Hazards Hun, Sch Basic Med, Sch Publ Hlth,Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Tokunou, Yoshihide; Long, Xizi; Okamoto, Akihiro] Int Ctr Mat Nanoarchitecton WPI MANA, Natl Inst Mat Sci, Tsukuba, Ibaraki 3050044, Japan.;[Tokunou, Yoshihide] Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki 3058577, Japan.;[Okamoto, Akihiro] Hokkaido Univ, Grad Sch Chem Sci & Engn, Sapporo, Hokkaido 0608628, Japan.
通讯机构:
[Akihiro Okamoto] I;International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan<&wdkj&>Graduate School of Chemical Sciences and Engineering, Hokkaido University, North 13 West 8, Kitaku, Sapporo, Hokkaido 060-8628, Japan
关键词:
circular dichroism;electron transport;exciton coupling;mechanobiology;multi-heme cytochrome C
作者机构:
[Yang, Shengyuan; Tan, Yan; Su, Wenen; Liu, Jinquan; Fan, Pengfei; Li, Qianji] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.;[Wu, Xiwen] Yueyang Cty Ctr Dis Control & Prevent, Yueyang 414100, Hunan, Peoples R China.;[Zhang, Xiaohuan] Yongding Dist Ctr Dis Control & Prevent, Zhangjiajie 427000, Hunan, Peoples R China.
通讯机构:
[Li, Qianji] D;Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, People's Republic of China.
作者机构:
[Yang, Yunyin; Lv, Yitao; Li, Jingyan; Zhang, Yi; Zhang, Min] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China.;[Wang, Jiacheng] Yangzhou Univ, Med Coll, Yangzhou 225009, Jiangsu, Peoples R China.;[Xiao, Xilin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Xilin Xiao] D;[Min Zhang] C;College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China<&wdkj&>Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China
作者机构:
[Yu, Wenzhan; Xiao, Xilin] Univ South China, Sch Pharmaceut Sci, Hengyang 421001, Hunan, Peoples R China.;[Li, Qian; Liao, Lifu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[He, Liqiong; Zhou, Renlong; Xue, Jinhua] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Jinhua Xue; Xilin Xiao] S;School of Public Health, University of South China, Hengyang 421001, Hunan, PR China<&wdkj&>School of Pharmaceutical Science, University of South China, Hengyang 421001, Hunan, PR China
作者机构:
[Pan, Jianming; Liu, Bangxiang; Hu, Panwang; Wang, Mengzhu; Niu, Xiangheng; Zhu, Hengjia] Jiangsu Univ, Inst Green Chem & Chem Technol, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China.;[Niu, Xiangheng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构:
[Xiangheng Niu] I;Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China
作者机构:
[Tang, Peng; Deng, Shuxiang; Liu, Ying; Feng, Shuidong; Yang, Fei; Tang, Yan] Univ South China, Sch Basic Med, Sch Publ Hlth, Hengyang Med Sch,Dept Epidemiol & Hlth Stat,Key L, Hengyang 421001, Peoples R China.;[Xu, Shuaishuai; Duan, Yanying; Shen, Minxue; Wei, Jia; Yang, Fei; Yang, Yue] Cent South Univ, Xiangya Sch Publ Hlth, Dept Social Med & Hlth Management, Hunan Prov Key Lab Clin Epidemiol, Changsha 410000, Peoples R China.;[Lu, Yao] Cent South Univ, Xiangya Hosp 3, Ctr Clin Pharmacol, Changsha 410000, Peoples R China.;[Pu, Yuepu; Liang, Geyu; Yang, Fei] Southeast Univ, Sch Publ Hlth, Key Lab Environm Med Engn, Minist Educ, Nanjing 210000, Peoples R China.;[Chen, Xiang; Shen, Minxue] Cent South Univ, Xiangya Hosp, Dept Dermatol, Changsha 410000, Peoples R China.
通讯机构:
[Minxue Shen] H;[Fei Yang] D;Hunan Provincial Key Laboratory of Clinical Epidemiology, Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha410000, China<&wdkj&>Department of Dermatology, Xiangya Hospital, Central South University, Changsha410000, China<&wdkj&>Department of Epidemiology and Health Statistics, The Key Laboratory of Typical Environmental Pollution and Health Hazards of Hunan Province, School of Basic Medicine, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>Hunan Provincial Key Laboratory of Clinical Epidemiology, Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha410000, China<&wdkj&>Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing210000, China
通讯机构:
[Zhaoyang Wu] S;State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People’s Republic of China
作者机构:
[Li, Yanfang; Si, Sisi; Li, Huimin; Liu, Song] Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha;410082, China;[Yang, Fei] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang;421001, China;[Huang, Feiyu; Wei, Jia] Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha
通讯机构:
[Fei Yang] H;[Huimin Li; Song Liu] I;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang 421001, PR China<&wdkj&>Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China