Highly sensitive and efficient fluorescent sensing for Hg2+ detection based on triple-helix molecular switch and exonuclease III-assisted amplification

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Zhong, Miao;Yang, Shengyuan;Chen, Lei;Liu, Chen;Shi, Jiao;...

通讯作者：

Liu, JQ

作者机构：

[Zhong, Miao; Yang, Shengyuan; Liu, Jinquan; Shi, Jiao; Li, Le; Chen, Lei; Liu, JQ; Liang, Hao; Xiao, Xilin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.

[Zhong, Miao; Yang, Shengyuan; Liu, Jinquan; Shi, Jiao; Li, Le; Chen, Lei; Liang, Hao; Xiao, Xilin] Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Hunan, Peoples R China.

[Liu, Chen] Univ South China, Chem & Chem Engn, Hengyang 421001, Peoples R China.

通讯机构：

[Liu, JQ ] U

Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.

语种：

英文

关键词：

Exonuclease III;Fluorescence;Mercury (Hg(2+));Thymine-mercury-thymine;Triple-helix molecular switch

期刊：

Analytica Chimica Acta

ISSN：

0003-2670

年：

2022

卷：

1205

页码：

339751

DOI：

10.1016/j.aca.2022.339751

基金类别：

CRediT authorship contribution statement Miao Zhong: Conceptualization, Methodology, Investigation, Validation, Data curation, Writing – original draft. Shengyuan Yang: Supervision, Writing – review & editing. Lei Chen: Investigation, Validation. Chen Liu: Investigation. Jiao Shi: Investigation. Hao Liang: Writing – review & editing. Xilin Xiao: Writing – review & editing. Le Li: Writing – review & editing. Jinquan Liu: Conceptualization, Supervision, Writing – review & editing, acquisition.

机构署名：

本校为第一且通讯机构

院系归属：

公共卫生学院

摘要：

Here, a novel fluorescent sensing for simple, highly sensitive and efficient detection of Hg2+ was developed as joint result of triple-helix molecular switch (THMS) and exonuclease III (Exo III)-assisted signal amplification. In this study, the special structure of THMS was used to realize efficient fluorescence quenching and excellent signal unit transformation to complete the output of signal FAM. In the absence of Hg2+, hairpin probe (HP) containing thymine-rich (T-rich) ssDNA strand can induce the dissociation of the THMS, causing FAM far a...

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Highly sensitive and efficient fluorescent sensing for Hg2+ detection based on triple-helix molecular switch and exonuclease III-assisted amplification

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