Ultrasensitive label-free electrochemical biosensor for detecting linear microcystin-LR using degrading enzyme MlrB as recognition element

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成果类型：

期刊论文

作者：

Li, Yanfang;Si, Sisi;Huang, Feiyu;Wei, Jia;Dong, Shengyi;...

通讯作者：

Yang, Fei(phfyang@csu.edu.cn)

作者机构：

[Li, Yanfang; Si, Sisi; Li, Huimin; Liu, Song] Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha

410082, China

[Yang, Fei] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang

421001, China

[Huang, Feiyu; Wei, Jia] Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha

通讯机构：

[Fei Yang] H

[Huimin Li; Song Liu] I

Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang 421001, PR China<&wdkj&>Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China

语种：

英文

关键词：

Electrochemical biosensor;Linear microcystin-LR;MlrB;Multi-walled carbon nanotube;Square wave voltammetry

期刊：

Bioelectrochemistry

ISSN：

1567-5394

年：

2022

卷：

144

页码：

108000

DOI：

10.1016/j.bioelechem.2021.108000

基金类别：

The work gratefully acknowledges financial support from the National Natural Science Foundation of China (No. 21975067 ), Hunan Province Excellent Youth Fund ( 2020JJ3053 ), National Natural Science Foundation of China ( 81773393 ), and Fundamental Research Funds for the Central Universities from Hunan University .

机构署名：

本校为通讯机构

院系归属：

公共卫生学院

摘要：

A label-free electrochemical biosensor was firstly constructed to detect linear microcystin-LR (L-MC-LR) with high sensitivity. Degradation enzyme MlrB was used as recognition element for specific recognition of L-MC-LR. The electrode was modified with –COOH functionalized multi-walled carbon nanotube to increase the specific surface area and improve the conductivity, which was then applied to immobilize MlrB. The electrochemical signal was changed with the reaction between MlrB and L-MC-LR, which was recorded by using square wave voltammetry....

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Ultrasensitive label-free electrochemical biosensor for detecting linear microcystin-LR using degrading enzyme MlrB as recognition element

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