Association of multi-metals exposure with intelligence quotient score of children: A prospective cohort study
作者：
Shen, Minxue;Zhang, Chengcheng;Yi, Xiping;Guo, Jian;Xu, Shuaishuai;...
期刊：
Environment International ,2021年155:106692 ISSN：0160-4120
通讯作者：
Shen, Minxue(shenmx1988@csu.edu.cn)
作者机构：
[Luo, Dan; Zhang, Chengcheng; Shen, Minxue] Cent South Univ, Xiangya Sch Publ Hlth, Dept Social Med & Hlth Management, Changsha, Hunan, Peoples R China.;[Shen, Minxue; Chen, Xiang] Cent South Univ, Xiangya Hosp, Dept Dermatol, Changsha, Hunan, Peoples R China.;[Zhang, Chengcheng; Guo, Jian; Xu, Shuaishuai; Yi, Xiping; Yang, Fei] Cent South Univ, Xiangya Sch Publ Hlth, Dept Occupat & Environm Hlth, Changsha, Hunan, Peoples R China.;[Huang, Zhijun] Cent South Univ, Xiangya Hosp 3, Ctr Clin Pharmacol, Changsha, Hunan, Peoples R China.;[He, Meian] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Dept Occupat & Environm Hlth, Wuhan, Hubei, Peoples R China.
通讯机构：
[Minxue Shen; Xiang Chen; Dan Luo; Fei Yang] D;Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha, China<&wdkj&>Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China<&wdkj&>Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha, China<&wdkj&>Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha, China<&wdkj&>Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical College, University of South China, Hengyang 421001, China<&wdkj&>Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
关键词：
Heavy metals;Bayesian kernel machine regression;Intelligence quotient;Children
摘要：
Background: Associations between most single metals and children's intelligence quotient (IQ) scores have been evaluated in previous studies. However, associations between multi-metal exposures and children's IQ scores have not been analyzed. Objectives: To assess the joint effects of lead (Pb), manganese (Mn), antimony (Sb), tin (Sn) and titanium (Ti) co-exposure on children's IQ scores. Methods: A prospective cohort study was conducted in Shimen and Huayuan, Hunan Province, China. Urine metals levels were measured by inductively coupled plasma mass spectrometry (ICP-MS) at baseline. Children's IQ scores were repeatedly measured at baseline and follow-up following the method of Raven's Standard Progressive Matrices (SPM) and standardized as z scores. We fitted linear regression models and Bayesian kernel machine regression (BKMR) models to investigate the associations of metal levels with children's IQ scores after adjusting for covariates. Results: A total of 633 participants aged 7–10 years completed the survey. Urinary Pb (β = -0.028, P = 0.022) and urinary Ti (β = -0.0003, P = 0.001) were inversely associated with children's IQ scores. The BKMR analyses revealed significant negative overall effects of the five metals on children's IQ scores when all the metals were above their median levels, while significant positive associations were shown when all the metal concentrations were below their median levels. The model also showed negative trends of Sn and Ti on children's IQ. Furthermore, Ti and Sn had a synergistic relationship, with a decline in IQ score when Sn exposure was relatively high. The urinary Sn concentration was significantly higher but the urinary Ti concentration was significantly lower in participants from the Shimen area than in those from the Huayuan area. Decreasing trends of the overall effects were observed in both the Shimen and Huayuan areas. Conclusion: Our findings revealed that multi-metal exposures caused a decline in children's IQ scores according to traditional linear regression models and the BKMR model. Our results provide some evidence of the association between multi-metal exposure and children's IQ. Meanwhile, interactions between multi-metal exposures on children's IQ should be given more attention. © 2021 The Authors
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英文
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Dual-signal amplification electrochemical sensing for the sensitive detection of uranyl ion based on gold nanoparticles and hybridization chain reaction-assisted synthesis of silver nanoclusters
作者：
Chen, Lei;Liu, Jinquan;Cao, Chen;Tang, Shuangyang;Lv, Changyin;...
期刊：
Analytica Chimica Acta ,2021年1184:338986 ISSN：0003-2670
通讯作者：
Li, Le
作者机构：
[Yang, Shengyuan; Liu, Ling; Liu, Jinquan; Li, Le; Chen, Lei; Lv, Changyin; Xiao, Xilin; Sun, Lin; Zhu, Bingyu] Univ South China, Hengyang Med Sch, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Yang, Shengyuan; Liu, Ling; Liu, Jinquan; Li, Le; Chen, Lei; Lv, Changyin; Xiao, Xilin; Sun, Lin; Zhu, Bingyu] Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Hunan, Peoples R China.;[Yang, Shengyuan; Liu, Ling; Liu, Jinquan; Li, Le; Chen, Lei; Lv, Changyin; Xiao, Xilin; Sun, Lin; Zhu, Bingyu] Univ South China, Key Lab Hlth Hazard Factors Inspect & Quarantine, Hengyang 421001, Hunan, Peoples R China.;[Cao, Chen] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Tang, Shuangyang] Univ South China, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Li, Le] U;Univ South China, Hengyang Med Sch, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
关键词：
Electrochemical sensing;Gold nanoparticles (AuNPs);Hybrid chain reaction (HCR);Silver nanoclusters (AgNCs);Uranyl ions (UO(2)(2+))
摘要：
Herein, a dual-signal amplification electrochemical sensing has been proposed for the ultrasensitive detection of uranyl ions (UO22+) by integration of gold nanoparticles (AuNPs) and hybridization chain reaction (HCR)-assisted synthesis of silver nanoclusters (AgNCs). In this sensing platform, AuNPs are used as an ideal signal amplification carrier, aiming at increasing the loads of UO22+-specific DNAzyme on the gold electrode. In the presence of UO22+, UO22+-specific DNAzyme can be activated, leading to the cleavage of substrate strands (S-DNA). Then, HCR is triggered to produce long dsDNA through hybridization the probe with the ssDNA on the electrode surface. As a result, an amplified electrochemical response can be detected by inserting a large amount of AgNCs generated in situ using dsDNA as template. Featured with amplification efficiency, good specificity and high sensitivity, the strategy could quantitatively detect UO22+ down to 6.2 pM with a linear calibration range from 20 pM to 5000 pM. The proposed sensing platform has been also successfully demonstrated the practical application of detecting UO22+, indicating that the developed method has the potential applications and can open up a new avenue for highly sensitive detection of UO22+ in environmental monitoring. © 2021 Elsevier B.V.
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英文
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Spider Toxin Peptide-Induced NIR Gold Nanocluster Fabrication for GSH-Responsive Cancer Cell Imaging and Nuclei Translocation
作者：
Tan, Huaxin;Liu, Sisi;He, Yaolin;Cheng, Guofeng;Zhang, Yu;...
期刊：
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY ,2021年9:780223 ISSN：2296-4185
通讯作者：
Hu, Lidan
作者机构：
[Tan, Huaxin; Hu, Lidan; Zhang, Yu; Cheng, Guofeng] Univ South China, Key Lab Ecol Environm & Crit Human Dis Prevent Hu, Dept Biochem & Mol Biol, Dept Educ,Sch Basic Med,Hengyang Med Sch, Hengyang, Peoples R China.;[Liu, Sisi] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Peoples R China.;[He, Yaolin] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Radiotherapy, Hengyang, Peoples R China.;[Wei, Xiaojie] Univ South China, Sch Pharm, Hengyang Med Sch, Hengyang, Peoples R China.
通讯机构：
[Hu, Lidan] U;Univ South China, Key Lab Ecol Environm & Crit Human Dis Prevent Hu, Dept Biochem & Mol Biol, Dept Educ,Sch Basic Med,Hengyang Med Sch, Hengyang, Peoples R China.
关键词：
NIR gold nanoclusters;spider toxin peptide;Cancer cell imaging;nuclei translocation;GSH-responsive
摘要：
Goldnanoclusters (GNCs) have become a promising nanomaterial for bioimaging because of their unique optical properties and biocompatibility. In this study, lycosin-I peptide, which possesses a highly selective anticancer activity by affecting the permeability of cancer cell membrane, was firstly modified for constructing fluorescent GNCs (LGNCs) for bioimaging of tumor cells. The obtained LGNCs exhibited strong near-infrared (NIR) fluorescence, which can be further enhanced by the peptide-induced aggregation and selectively stained three cancerous cell lines over normal cell lines with low intrinsic toxicity. After uptake by tumor cells, LGNC aggregates can be depolymerized into ultrasmall nanoclusters by high-level glutathione (GSH) and realize the nuclear targeting translocation. Collectively, our work suggests the potential of natural active biomolecules in designing NIR fluorescent GNCs for bioimaging. Copyright © 2021 Tan, Liu, He, Cheng, Zhang, Wei and Hu.
语种：
英文
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Exposure of Chinese adult females to parabens from personal care products: Estimation of intake via dermal contact and health risks
作者：
Li, Chun;Zhao, Yang;Liu, Shan;Yang, Dongfeng;Ma, Huimin;...
期刊：
Environmental Pollution ,2021年272:116043 ISSN：0269-7491
通讯作者：
Lu, Shaoyou
作者机构：
[Li, Chun; Zhao, Yang; Lu, Shaoyou; Liu, Shan] Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Guangzhou 510275, Peoples R China.;[Kang, Li; Yang, Dongfeng; Zhu, Zhou] Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Peoples R China.;[Yang, Dongfeng] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Ma, Huimin] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China.;[Ma, Huimin] Chinese Acad Sci, Guangzhou Inst Geochem, Guangdong Key Lab Environm Protect & Resources Ut, Guangzhou 510640, Peoples R China.
通讯机构：
[Lu, Shaoyou] S;Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Guangzhou 510275, Peoples R China.
关键词：
Adult females;Health risk;Intake;Parabens;Personal care products
摘要：
Capsule: Leave-on PCPs, especially sunscreen, body lotion, and mask, constituted the main sources of paraben exposure via dermal contact for Chinese adult females. © 2020 Elsevier LtdParabens are added into foodstuffs, pharmaceuticals and personal care products (PCPs) as additives extensively due to their excellent antiseptic and antibacterial effects. In the past decade, parabens have raised great concerns on their potential harm to humans. Existing studies have suggested positive correlations between PCP application and urinary paraben concentrations in females, but little is known about paraben exposure levels and health risks arising from PCP use. In this study, 150 PCP samples covering eleven categories were collected from South China and measured for the concentrations of five parabens, including methyl paraben (MeP), ethyl paraben (EtP), propyl paraben (PrP), butyl paraben (BuP) and benzyl paraben (BeP). Parabens were widely detected in PCPs, with a detection frequency of 100%, 99.3%, 80.0%, 74.0% and 13.3%, for MeP, EtP, PrP, BuP and BeP, respectively. The median concentration of Σ5parabens was 126 μg/g with a range of 6.38–424 μg/g across all PCP samples. The contents of MeP, EtP and PrP measured in leave-on PCPs were obviously higher than those in the rinse-off ones (p < 0.05). MeP and PrP were the main paraben analogues, together accounting for 93.6% of Σ5parabens in all PCPs. The daily intakes of parabens through dermal absorption by Chinese adult females estimated by measurements obtained in the present study were 0.15 and 83.2 μg/kg-bw/day on basis of the application of rinse-off and leave-on PCPs, respectively. Among the eleven categories, sunscreen, body lotion and mask constituted the main exposure sources of parabens to females. The hazard quotients of parabens were far less than 1, indicating no considerable health risk for Chinese adult females. © 2020 Elsevier Ltd
语种：
英文
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Mechanism of recombinant bacteria adsorb UO22+ under culture condition
作者：
Shu, Yangzhen;Li, Shanshan;Xie, Jingxi;Guo, Kexin;Cheng, Conghui;...
期刊：
Enzyme and Microbial Technology ,2021年151:109920 ISSN：0141-0229
通讯作者：
Peng, Guowen(pgwnh78@163.com);Xiao, Fangzhu(xfzhunh@163.com)
作者机构：
[Shu, Yangzhen; Peng, Guowen] Univ South China, Sch Environm Protect & Safety Engn, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Fangzhu; Cheng, Conghui; Guo, Kexin; Chen, Luoyao; Li, Shanshan] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Xie, Jingxi; Peng, Guowen] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Peng, G.; Xiao, F.] S;School of Environmental Protection and Safety Engineering of University of South ChinaChina;School of Public Health, China
关键词：
Biosorption;Mechanism;Uranium
摘要：
Research on the ability and mechanism of genetically recombinant E. coli DH5α containing DSR A gene to enrich uranium under culture conditions provides a theoretical basis for the application of the bacteria in the treatment of uranium pollution. By exploring the influence of factors such as the initial concentration of uranium, culture time, and inoculation amount on the characteristics of uranium enrichment in genetically recombinant E. coli, using FTIR, SEM-EDS, XPS and XRD explore the mechanism of uranium-enriched bacteria. The results showed that when initial UO22+ concentration reach 600 mg/L, E. Coli D1 could not survived, indicated that the maximum tolerance concentration is lower than 600 mg/L. While concentration between 0∼500 mg/L, strains D1 can grow normally and has the ability to enrich uranium. In the prime stage, strains D1 resist toxics through release inorganic phosphates to precipitate UO22+ on cell wall, after 96 h, most UO22+ were transferred into cytoplasm and metabolized into U(IV) which is less toxic. In the metabolize process, all groups involved in metabolizing UO22+, especially protein contain groups like hydroxyl, amine and carboxyl paly a huge role. It shows that within a certain concentration rage, strains D1 has a good enrichment effect on uranium under culture conditions. © 2021 Elsevier Inc.
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英文
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Control of Liquid Crystal Microarray Optical Signals Using a Microspectral Mode Based on Photonic Crystal Structures
作者：
Wang, Shihong;Qi, Yue;Chen, Qianshan;Zhang, Guannan;Liu, Bing;...
期刊：
Analytical Chemistry ,2021年93(34):11887-11895 ISSN：0003-2700
通讯作者：
Wu, Zhaoyang(zywu@hnu.edu.cn)
作者机构：
[Liu, Bing; Wu, Zhaoyang; Zhou, Jun; Yu, Ruqin; Zhang, Guannan; Xiao, Fubing; Wang, Shihong; Qi, Yue; Chen, Qianshan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Xiao, Fubing] Univ South China, Coll Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.
通讯机构：
[Zhaoyang Wu] S;State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People’s Republic of China
摘要：
Herein, a novel liquid crystal microarray (LCM) film with optical regulation ability is first constructed by combining liquid crystals (LC) and the highly ordered microporous structure of inverse opal photonic crystals (IOPhCs). The LCM films are fabricated by infiltrating LC molecules into the LC polymer with the structure of IOPhCs, and their properties are very different from those without the LC. Interestingly, the optical property of LCM films can be controlled by changing the orientation of LC molecules, which varies with the interfacial force. In combination with polarization images, spectral reflection peak, circular dichroism spectra, potential difference, and fluorescence images of LCM films, the mechanism of this change is investigated. It is found that the exposed basic group of single-stranded DNA is the key to the change of the optical property of LC microarrays. Meanwhile, the optical signals of LC microarrays based on the PhCs provide a novel LC signal mode for an LC sensing system (microspectral signal mode), and it can be recorded by a fiber-optic spectrometer, which is a great improvement on LC sensing signals. Therefore, the LC microarray sensing signal can be used for accurate analysis of targets by the change of the reflection peak intensity of PhCs. When the LC molecules are induced by different aptamers, the LC microarray sensing interface can be further used for the determination of different targets, such as cocaine and Hg2+. The research on LCM films is of significant value for the development of LC sensing technology and also shows great application prospects in biochemical sensing fields. © 2021 American Chemical Society.
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英文
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Effects of environmental contaminants in water resources on nonalcoholic fatty liver disease
作者：
Zheng, Shuilin;Yang, Yue;Wen, Cong;Liu, Wenya;Cao, Linghui;...
期刊：
Environment International ,2021年154:106555 ISSN：0160-4120
通讯作者：
Yang, Fei(yangfeilong@126.com)
作者机构：
[Chen, Jihua; Liu, Wenya; Zheng, Shuilin; Wen, Cong; Feng, Xiangling; Cao, Linghui; Yang, Yue; Yang, Fei] Cent South Univ, Xiangya Sch Publ Hlth, Hunan Prov Key Lab Clin Epidemiol, Changsha 410078, Peoples R China.;[Wang, Hui; Yang, Fei; Tang, Yan] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.;[Tian, Li; Wang, Xiaoyan] Cent South Univ, Xiangya Hosp 3, Dept Gastroenterol, Changsha, Peoples R China.;[Yang, Fei] Southeast Univ, Key Lab Environm Med Engn, Minist Educ, Sch Publ Hlth, Nanjing 210009, Peoples R China.
通讯机构：
[Fei Yang] H;Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha 410078, China<&wdkj&>Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang 421001, China<&wdkj&>Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health Southeast University, Nanjing 210009, China
关键词：
Environmental contaminants;Water resources;Lipid metabolism;Nonalcoholic fatty liver disease (NAFLD)
摘要：
The global prevalence of nonalcoholic fatty liver disease (NAFLD) has been increasing rapidly in recent years, which is now estimated to be over 25%. NAFLD is one of the most common chronic liver diseases in the world. At present, with the rapid development of economy and industrialization, many chemicals are released into the environment. These chemical contaminants in the environment might cause harm to human health and result in lipid metabolism disorder during long-term exposure. Moreover, the incentive of many NAFLD cases is unknown, and the environmental risk factors of NAFLD need to be urgently identified. Hence, we focus on the impacts of several popular environmental contaminants in water environment on the development and progression of NAFLD. These contaminants mainly include microcystins (MCs), disinfection by-products (DBPs), heavy metals (HMs), dioxins and polychlorinated biphenyls (PCBs). Through analyzing a great many epidemiological and toxicological studies, we have found positive associations between NAFLD and chronic exposure to these contaminants at the environmental levels. This review may enhance the understanding of liver damage caused by environmental pollutants, which are considered as tangible environmental risk factors for NAFLD. © 2021 The Author(s)
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英文
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Optimization of microcystin biodegradation by bacterial community YFMCD4 using response surface method
作者：
Guo, Jian;Wei, Jia;Huang, Feiyu;Massey, Isaac Yaw;Luo, Jiayou;...
期刊：
Chemosphere ,2021年274:129897 ISSN：0045-6535
通讯作者：
Yang, Fei(yangfeilong@126.com)
作者机构：
[Guo, Jian] Cent South Univ, Xiangya Stomatol Hosp, Changsha 410008, Hunan, Peoples R China.;[Yang, Fei] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.;[Guo, Jian] Cent South Univ, Xiangya Sch Stomatol, Changsha 410008, Hunan, Peoples R China.;[Wei, Jia; Massey, Isaac Yaw; Yang, Fei; Huang, Feiyu; Luo, Jiayou] Cent South Univ, Xiangya Sch Publ Hlth, Hunan Prov Key Lab Clin Epidemiol, Changsha 410078, Peoples R China.
通讯机构：
[Fei Yang] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang, 421001, China<&wdkj&>Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha, 410078, China
关键词：
Bacterial community structure;Microcystin-LR biodegradation;Response surface methodology;mlrA
摘要：
The increasing production of microcystin-LR (MC-LR) causing animal and human health issues is found in eutrophic water bodies, marine habitats and desert environments. The health threat posed by MC-LR has led to the establishment of World Health Organization's water guideline value of 1 μg/mL. Combating this has increased the search for cost-effective approach to degrade MC-LR. The study aimed to optimize the MC-degrading environmental factors of bacterial community YFMCD4. Response surface methodology (RSM) was employed to evaluate the influence of varying temperatures, pH and initial MC-LR concentration on the biodegradation efficiency of MC-LR by bacterial community YFMCD4. The optimal MC-LR biodegradation environmental factors were found to be 30 °C, pH 7 and 2 μg/mL initial MC-LR. The biodegradation rate reached 100% after 10 h. YFMCD4 mainly consisted of genera Alacligenes, Sphingobacterium and Pseudomonas using High-throughput pyrosequencing technology. The mlrA gene encoding MlrA enzyme considered most important for MC-LR biodegradation was obtained from YFMCD4. Data demonstrated that the bacterial structure and biodegradation efficiency of YFMCD4 varied with the change of environmental factors including temperature, pH and MC-LR concentrations. RSM is considered a good method to examine the optimal biodegradation environmental conditions for MC-LR. To date, RSM and High-throughput pyrosequencing technology are employed to optimize the biodegradation conditions (30 °C, pH 7 and 2 μg/mL initial MC-LR) and analyze the structure of bacterial community for the first time. © 2021 Elsevier Ltd
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英文
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A novel liquid crystal sensing platform for highly selective UO22+detection based on a UO22+-specific DNAzyme
作者：
Hu, Congcong;Li, Ping;Wu, Zhaoyang;Fan, Fengfei;Qian, Duo;...
期刊：
ANALYTICAL METHODS ,2021年13(40):4732-4738 ISSN：1759-9660
通讯作者：
Yang, Shengyuan(yangshyhy@126.com);Xiao, Fubing(xiaofubing2008@163.com)
作者机构：
[Hu, Congcong; Yang, Shengyuan; Fan, Fengfei; Xiao, Fubing; Qian, Duo; Yi, Yuxin] Univ South China, Hengyang Med Sch, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Wu, Zhaoyang; Li, Ping; Xiao, Fubing] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.
通讯机构：
[Hu, Congcong] C;College of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, People's Republic of China.
摘要：
A label-free and selective sensor was established for uranyl ion (UO22+) detection based on a UO22+-dependent DNAzyme and liquid crystals (LCs). In the presence of UO22+, the substrate chains can be cleaved at the rA site by the DNAzyme strands. The cleaved products released from the DNAzyme strand will hybridize with the capture probes that are fixed on the LC sensing substrate to form double strands. The formation of double strands would disturb the original orientation and induce the rearrangement of liquid crystal molecules, resulting in the polarization images changing from uniform black to bright. Attributed to the specificity of the DNAzyme and the optical signal of the LC, a highly selective and label-free method was established with a detection limit of 25 nM. This approach showed satisfactory analytical performance and offered an inspiring platform for detecting other radioactive elements. This journal is © The Royal Society of Chemistry.
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英文
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Exposure to heavy metals and its association with DNA oxidative damage in municipal waste incinerator workers in Shenzhen, China
作者：
Yang, Dongfeng;Liu, Yanling;Liu, Shan;Li, Chun;Zhao, Yang;...
期刊：
Chemosphere ,2020年250(Jul.):126289 ISSN：0045-6535
通讯作者：
Lu, Shaoyou
作者机构：
[Li, Le; Yang, Dongfeng] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Li, Chun; Zhao, Yang; Lu, Shaoyou; Liu, Shan] Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Guangzhou 510275, Guangdong, Peoples R China.;[Liu, Yanling] Nanshan Dist Ctr Dis Control & Prevent, Shenzhen 518054, Peoples R China.;[Yang, Dongfeng] Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Peoples R China.
通讯机构：
[Lu, Shaoyou] S;Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Guangzhou 510275, Guangdong, Peoples R China.
关键词：
Waste incineration;Heavy metals;Oxidative stress;Urine
摘要：
Burning municipal waste produces a great deal of harmful heavy metals, which may lead to elevated exposure in incinerator workers and residents living nearby. However, relevant human exposure studies remain scarce, especially in China. This study aimed to determine the concentrations of ten heavy metals in urine of incinerator workers (n = 119, as the exposed group) and residents living nearby (n = 215, as the control group) from Shenzhen (China), and explore the associations between heavy metal exposure and DNA oxidative stress (indicated by 8-hydroxy-2'-deoxyguanosine or 8-OHdG) in humans. The median urinary concentrations of manganese (Mn), iron (Fe), chromium (Cr), arsenic (As), selenium (Se) and 8-OHdG in the exposed group were significantly higher than those in the control group (p < 0.05), suggesting occupational exposure elevated heavy metal intake for the incinerator workers. Nevertheless, there is no correlation statistically significant between the concentrations of any heavy metal and 8-OHdG in urine in the exposed group, indicating heavy metals releasing from waste incineration were not important factors to induce DNA oxidative stress. To our knowledge, this study firstly reported the concentrations of heavy metal in urine and their associations with DNA oxidative damage in waste incinerator workers in Shenzhen, China. (C) 2020 Elsevier Ltd. All rights reserved.
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英文
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Trace elements in shellfish from Shenzhen, China: Implication of coastal water pollution and human exposure
作者：
Liu, Shan;Liu, Yanling;Yang, Dongfeng;Li, Chun;Zhao, Yang;...
期刊：
Environmental Pollution ,2020年263(Aug. Pt.B):114582.1-114582.8 ISSN：0269-7491
通讯作者：
Lu, Shaoyou
作者机构：
[Li, Chun; Zhao, Yang; Lu, Shaoyou; Liu, Shan] Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Guangzhou 510275, Peoples R China.;[Liu, Yanling] Nanshan Dist Ctr Dis Control & Prevent, Shenzhen 518054, Peoples R China.;[Yang, Dongfeng] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Ma, Huimin] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China.;[Ma, Huimin] Chinese Acad Sci, Guangzhou Inst Geochem, Guangdong Key Lab Environm Protect & Resources Ut, Guangzhou 510640, Peoples R China.
通讯机构：
[Lu, Shaoyou] S;Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Guangzhou 510275, Peoples R China.
关键词：
Trace elements;Shellfish;Estimated daily intake;Health risk
摘要：
Shellfish constitute an important component of human diet, especially for those living in coastal regions. Shellfish have attracted extensive attention due to high enrichment of heavy metals. The aims of this study were to investigate the levels of trace elements in shellfish from coastal waters of Shenzhen, China and to assess human intake risks. Nine elements, including chromium (Cr), copper (Cu), iron (Fe), zinc (Zn), manganese (Mn), selenium (Se), cadmium (Cd), arsenic (As) and lead (Pb) were measured in 216 shellfish samples from eight species. Their concentrations (based on wet weight) were: Cr (0.28–21.4 mg kg−1), Cu (1.40–158 mg kg−1), Fe (16.5–5387 mg kg−1), Zn (11.1–847 mg kg−1), Mn (1.33–422 mg kg−1), Se (0.15–11.8 mg kg−1), Cd (0.02–18.4 mg kg−1), Pb (<LOQ-10.9 mg kg−1) and As (2.24–95.5 mg kg−1), relatively greater than those reported in shellfish from other locations of China. Crassostrea ariakensis and Babylonia areolata were found to enrich As and Cd, respectively. The target hazard quotient (THQ) values of Cd and As were more than 1, suggesting considerable health risks from the consumption of shellfish of this zone. To our knowledge, this is the first study to assess the human risk exposure to trace elements via shellfish consumption in South China. © 2020 Elsevier LtdResidents in Shenzhen, China have been subjected to considerable health risks of As and Cd via shellfish consumption. © 2020 Elsevier Ltd
语种：
英文
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Spatial variation of fipronil and its derivatives in tap water and ground water from China and the fate of them during drinking water treatment in Wuhan, central China
作者：
Shi, Lisha;Chen, Li;Wan, Yanjian* ;Zeng, Huaicai;Xia, Wei*
期刊：
Chemosphere ,2020年251(Jul.):126385 ISSN：0045-6535
通讯作者：
Wan, Yanjian;Xia, Wei
作者机构：
[Xia, Wei; Shi, Lisha; Chen, Li] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Key Lab Environm & Hlth HUST,Minist Educ, Wuhan 430030, Hubei, Peoples R China.;[Xia, Wei; Shi, Lisha; Chen, Li] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Minist Environm Protect, Wuhan 430030, Hubei, Peoples R China.;[Xia, Wei; Shi, Lisha; Chen, Li] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, State Key Lab Environm Hlth Incubat, Wuhan 430030, Hubei, Peoples R China.;[Wan, Yanjian] Wuhan Ctr Dis Prevent & Control, Inst Environm Hlth, Wuhan 430015, Hubei, Peoples R China.;[Zeng, Huaicai] Univ South China, Sch Publ Hlth, Dept Prevent Med, 28 Western Changsheng Rd, Hengyang 421001, Peoples R China.
通讯机构：
[Wan, Yanjian] W;[Xia, Wei] H;Wuhan Ctr Dis Prevent & Control, Inst Environm Hlth, Wuhan 430015, Hubei, Peoples R China.;Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Wuhan 430030, Peoples R China.
关键词：
Fipronil;Insecticide;Yangtze river;Contamination;Removal
摘要：
Fipronil (FP) is an emerging insecticide, however, its occurrence in drinking water and source water is limited. In this work, a total of 789 tap water and 95 ground water samples were collected from China in June 2019 in order to assess occurrence of FP and its derivatives (FPs). FPs were also analyzed in source, treated (n = 10, July), and tap water samples (n = 81, July and October 2019) originating from the central Yangtze River and its tributary, the Hanshui River in Wuhan. The sum concentrations of FPs (ΣFPs) in the tap water in China ranged from not detected (ND) to 5.07 (median: 0.03 ng/L), with FP found in 55.3% of the samples, and other targets ≤ 50.0%. Significant regional variations in the ΣFPs values were found between East China (75th percentile: 0.31 ng/L) and Northwest China (0.04), as well as between East China and North China (0.04). Similar ΣFPs values were found for ground water and tap water. The estimated daily intake of ΣFPs via water ingestion was below 200 pg/kg-bw/day for all age groups and was lower than the reference dose for FP (0.2 μg/kg-bw/day). Additionally, FPs were found in all of the source water samples collected in Wuhan with concentrations in the range of 0.84–2.72 ng/L for ΣFPs (median: 2.39). Most of these FPs were removed during water treatment. Higher concentration of ΣFPs in tap water was observed in July (median: 0.04 ng/L) compared to that in October (ND). This is the first study on the occurrence of FPs in the Yangtze River, the fate of FPs during the tap water treatment, and the regional distribution of FPs in tap water from China. © 2020 Elsevier Ltd
语种：
英文
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基于裂开型核酸适体非标记荧光法检测ATP <&wdkj&>A New Label-Free Fluorometric Assay for ATP Based on Split Aptamer
作者：
Li Fei-fei;Lu Yi-song;Yang Sheng-yuan* ;Lin Xi;Chen Wei;...
期刊：
光谱学与光谱分析 ,2019年39(9):2769-2773 ISSN：1000-0593
通讯作者：
Yang Sheng-yuan
作者机构：
[Li Fei-fei; Chen Wei; Yang Sheng-yuan; Lin Xi; Xiao Fu-bing; Liang Hao; Liu Can] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Lu Yi-song] Univ South China, Affiliated Nanhua Hosp, Hengyang 421001, Peoples R China.;[Li Fei-fei; Yang Sheng-yuan; Lin Xi; Xiao Fu-bing; Liang Hao; Liu Can] Key Lab Hengyang Hlth Hazard Factors Inspect & Qu, Hengyang 421001, Peoples R China.
通讯机构：
[Yang Sheng-yuan] U;[Yang Sheng-yuan] K;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;Key Lab Hengyang Hlth Hazard Factors Inspect & Qu, Hengyang 421001, Peoples R China.
关键词：
裂开型核酸适体;单壁碳纳米管;荧光
摘要：
基于裂开型核酸适体序列短、能有效降低因探针形成二级结构产生假阳性信号等优点,选择裂开型核酸适体作为特异性识别探针,核酸染料噻唑橙(TO)为信号探针,用单壁碳纳米管(SWCNTs)降低背景信号,利用“适配体-目标分子-适配体”的“三明治”夹心方式,建立了一种检测ATP的新方法。在pH 8.0的Tris-HCl缓冲溶液中,裂开成两段的ATP适体特异性识别ATP分子,生成稳定的“适配体-ATP-适配体”复合结构。单壁碳纳米管对该复合结构的吸附力较弱,因此该复合物游离在溶液中,TO与其结合而产生强荧光。当不存在ATP时,核酸适体探针以单链状态存在,可通过π—π共轭作用结合到SWCNTs表面,进而不能与TO结合,TO游离在溶液中荧光非常微弱。反应体系中ATP浓度越高,形成的“适配体-ATP-适配体”夹心识别结构复合物越多,检测到的荧光强度越大,据此实现对ATP的检测。在优化实验条件下,在最大荧光发射波长550nm处,ATP的浓度在9.0×10~(-9)~1.0×10~(-7) mol·L~(-1)范围内与ΔF/F0值成线性关系,r=0.996 4。该方法加标回收率为95.2%~104%,相对标准偏差(RSD)为1.02%~4.54%,检出限达到2.67×10~(-9) mol·L~(-1)。该方法基于功能核酸对目标物亲合力强、选择识别性高的特点,对ATP的检测表现出很好的选择性,实验结果表明,当相对误差控制在±5%以内时,200倍的UTP,GTP和CTP均不干扰 ATP的测定。另外,该方法操作简单、快速、无需标记、灵敏准确,可用于血清样品中ATP的测定,在快速检测小分子物质领域中有较好的应用前景。 <&wdkj&>A novel Label-Free Fluorometric Assay based on the recombination of split aptamer chip was developed for thedetection of adenosine triphosphate(ATP).In this strategy,the split aptamer was selected as a specific capture probe for the split two fragments aptamers could specifically form a ternary assembly in the presence of ligand and the two separate oligonucleotides lack secondary structures,thus not yielding false-positive or nonspecific signals,while the Thiazole orange(TO),an almost nonfluorescence dye in buffer solution,was used as signal probe,and the single-walled carbon nanotubes(SWCNTs)was applied to reduce the background signals.In the pH 8.0Tris-HCl buffer solution,those two split aptamer fragments will be combined with each other to form a stable“aptamer-ATP-aptamer”composite structure upon interacting with its target ATP.The“sandwich” structure can't wrap the sidewalls of the SWCNTs and is freed in solution,and TO shows agreat fluorescence enhancement when binding to the“aptamer-ATP-aptamer”composite structure.In the absence of ATP,the split aptamers,existing in a singlestranded state,bind to the surface of the SWCNTs via aπ—π-conjugate interaction,and TO shows weak fluorescence because “sandwich”structure is not formed.In the system,the higher the ATP concentration is,the more the“aptamer-ATP-aptamer” sandwich recognition structure complex obtained,sois the fluorescence.Under the optimized experimental conditions,the ATP concentration in the range from 9.0×10~(-9) mol·L~(-1) to 1.0×10~(-7) mol·L~(-1) was linear with theΔF/F0value at the maximum fluorescence emission wavelength of 550nm,r=0.996 4,with a low detection limit of 2.67×10~(-9) mol·L~(-1).The recoveries of the method were 95.2%~104%,and the relative standard deviation(RSD)was 1.02%~4.54%,respectively.Based on the specific molecular recognition and high affinity of twosplit aptamers,the reaction product was shown that a“turn-on”fluorescence response to ATP with good selectivity,only a slight fluorescence change could be observed by GTP,CTP,and UTP(at a 200-fold higher concentration than that of ATP),indicating that UTP,CTP,and GTP could not interact with P1and P1to initiate the reaction.The method is simple,rapid,free-label,sensitive and accurate,and can be used for the determination of ATP in serum samples.Therefore,the present strategy has a great potential application prospect in the field of rapid detection of small molecular substances.
语种：
中文
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An Ultrasensitive Colorimetric Strategy for Detection of Cadmium Based on the Peroxidase-like Activity of G-Quadruplex-Cd(II) Specific Aptamer.
作者：
Zuou, Bin* ;Chen, Ya-Ting;Yang, Xin-Yi;Wang, Yong-Sheng;Hu, Xi-Jiang;...
期刊：
Analytical Sciences ,2019年35(3):277-282 ISSN：0910-6340
通讯作者：
Zuou, Bin;Suo, Qing-Li
作者机构：
[Suo, Qing-Li; Zuou, Bin; Hu, Xi-Jiang] Huazhong Univ Sci & Technol, Wuhan Maternal & Child Healthcare Hosp, Wuhan Childrens Hosp, Tongji Med Coll, 100 Hong Kong Rd, Wuhan 430016, Hubei, Peoples R China.;[Yang, Xin-Yi; Chen, Ya-Ting; Wang, Yong-Sheng] Univ South China, Coll Publ Hlth, 28 Chang Sheng West Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zuou, B; Suo, QL] H;Huazhong Univ Sci & Technol, Wuhan Maternal & Child Healthcare Hosp, Wuhan Childrens Hosp, Tongji Med Coll, 100 Hong Kong Rd, Wuhan 430016, Hubei, Peoples R China.
关键词：
Cd(II)-specific aptamer;cadmium;G-quadruplex;absorbance;conformation switching
摘要：
We rationally designed an ultrasensitive and label-free sensing platform for determination of cadmium (Cd). The sensing platform contains G-quadruplex-Cd(II) specific aptamer (GCDSA) constructed by incorporating G-rich sequence at the end of 5' and the critical domain of the Cd-4 aptamer. GCDSA designed act as both a special recognition sequence for Cd2+ and a signal DNAzyme. In absence of Cd2+, GCDSA may mainly exist in a random coil sequence. Upon addition of Cd2+, GCDSA could probably be induced to fold into a G-quadruplex structure. The generation of plentiful active G-quadruplex interacts with hemin to form a peroxidase-like DNAzyme, leading to increased absorbance signal of the sensing system. ΔA was directly proportional to the two segments of concentrations for Cd2+, with the detection of limit of 0.15 nM. The proposed method avoids the labeled oligonucleotides and allows directly quantitative analysis of the samples by cheap instruments, with an excellent dynamic range. © 2019 The Japan Society for Analytical Chemistry.
语种：
英文
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The size-dependent genotoxic potentials of titanium dioxide nanoparticles to endothelial cells
作者：
Liao, Fen;Chen, Lingying;Liu, Yuanfeng;Zhao, Dongting;Peng, Wenyi;...
期刊：
ENVIRONMENTAL TOXICOLOGY ,2019年34(11):1199-1207 ISSN：1520-4081
通讯作者：
Feng, Shaolong
作者机构：
[Liao, Fen; Liu, Yuanfeng; Zhao, Dongting; Peng, Wenyi; Wang, Wuxiang; Feng, Shaolong] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China;[Chen, Lingying] Univ South China, Affiliated Hosp 1, Hengyang, Peoples R China;[Wang, Wuxiang; Feng, Shaolong] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou, Guangdong, Peoples R China
通讯机构：
[Feng, Shaolong] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
endothelial cell (EC);genotoxicity;oxidative stress;titanium dioxide nanoparticle (TiO2-NP)
摘要：
Despite intensive research activities, there are still many major knowledge gaps over the potential adverse effects of titanium dioxide nanoparticles (TiO2-NPs), one of the most widely produced and used nanoparticles, on human cardiovascular health and the underlying mechanisms. In the present study, alkaline comet assay and cytokinesis-block micronucleus test were employed to determine the genotoxic potentials of four sizes (100, 50, 30, and 10 nm) of anatase TiO2-NPs to human umbilical vein endothelial cells (HUVECs) in culture. Also, the intracellular redox statuses were explored through the measurement of the levels of reactive oxygen species (ROS) and reduced glutathione (GSH) with kits, respectively. Meanwhile, the protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) were also detected by western blot. The results showed that at the exposed levels (1, 5, and 25 mu g/mL), all the four sizes of TiO2-NPs could elicit an increase of both DNA damage and MN frequency in HUVECs in culture, with a positive dose-dependent and negative size-dependent effect relationship (T100 < T50 < T30 < T10). Also, increased levels of intracellular ROS, but decreased levels of GSH, were found in all the TiO2-NP-treated groups. Intriguingly, a very similar manner of dose-dependent and size-dependent effect relationship was observed between the ROS test and both comet assay and MN test, but contrary to that of GSH assay. Correspondingly, the levels of Nrf2 protein were also elevated in the TiO2-NP-exposed HUVECs, with an inversely size-dependent effect relationship. These findings indicated that induction of oxidative stress and subsequent genotoxicity might be an important biological mechanism by which TiO2-NP exposure would cause detrimental effects to human cardiovascular health.
语种：
英文
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Colorimetric detection of Cr(vi) using silver nanoparticles functionalized with PVP
作者：
He, Shunzhen;Lin, Xi;Liang, Hao;Xiao, Fubing;Li, Feifei;...
期刊：
ANALYTICAL METHODS ,2019年11(45):5819-5825 ISSN：1759-9660
通讯作者：
Yang, Shengyuan;Liu, Yong
作者机构：
[He, Shunzhen; Yang, Shengyuan; Liang, Hao; Xiao, Fubing; Fan, Pengfei; Liu, Can; Lin, Xi; Li, Feifei] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Yang, Shengyuan; Liang, Hao; Xiao, Fubing; Fan, Pengfei; Liu, Can; Lin, Xi; Li, Feifei] Key Lab Hengyang Hlth Hazard Factors Inspect & Qu, Hengyang 421001, Peoples R China.;[Liu, Yong] Hunan Prov Engn Res Ctr Radioact Control Technol, Hengyang 421001, Peoples R China.;[Liu, Yong] Hunan Prov Engn Technol Res Ctr Uranium Tailings, Hengyang 421001, Peoples R China.
通讯机构：
[Yang, Shengyuan] U;[Yang, Shengyuan] K;[Liu, Yong] H;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;Key Lab Hengyang Hlth Hazard Factors Inspect & Qu, Hengyang 421001, Peoples R China.
摘要：
As a priority pollutant in the environment, hexavalent chromium (Cr(vi)) has received increasing attention. In this work, a safe, simple, low-cost, effective and sensitive colorimetric strategy for the determination of Cr(vi) was introduced based on polyvinylpyrrolidone (PVP) functionalized silver nanoparticles (PVP-AgNPs). The prepared PVP-AgNPs had good dispersibility, and exhibited a state change from dispersed to aggregated when Cr(vi) was present in pH 5.5 PBS buffer due to the special and strong coordination between PVP and Cr(vi), accompanied by a color change from bright yellow to orange. Under the optimized conditions, the concentration of Cr(vi) in the range of 1.0 × 10-7 M to 2.4 × 10-6 M showed a good linear relationship with the ratio of the absorbance at 530 nm to that at 390 nm (A530 nm/A390 nm), and the limit of detection (LOD) was 34 nM. The method also showed good precision and high repeatability in determining Cr(vi) in real water samples such as pond water, tap water and river water. The recoveries ranged from 93.5% to 104.3%, indicating the method can be used for an extensive range of practical applications. © The Royal Society of Chemistry.
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英文
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基于氧化石墨烯模拟酶比色法检测汞离子
作者：
Lin Xi;Lu Yi-song;Yang Sheng-yuan* ;Liu Lu-qun;Li Fei-fei;...
期刊：
光谱学与光谱分析 ,2018年38(10):3188-3191 ISSN：1000-0593
通讯作者：
Yang Sheng-yuan
作者机构：
[Li Fei-fei; Yang Sheng-yuan; Liu Lu-qun; He Shun-zhen; Lin Xi] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Lu Yi-song] Univ South China, Affiliated Nanhua Hosp, Hengyang 421001, Peoples R China.
通讯机构：
[Yang Sheng-yuan] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
氧化石墨烯;模拟酶;比色法;汞离子
摘要：
基于富T碱基序列能特异性识别Hg~(2+)、氧化石墨烯(GO)对单链DNA(ssDNA)和T-Hg~(2+) -T复合物的亲和力不同以及GO自身具有的模拟酶催化性能,构建了一种可视化检测水样中痕量Hg~(2+)的新方法。在pH 4.0的NaAc-HAc缓冲溶液中,通过π—π堆积作用力,ssDNA可以吸附在GO表面,致使GO的类过氧化物酶活性减弱,从而催化H_2O_2氧化3,3',5,5'-四甲基联苯胺(TMB)产生的蓝色产物减少,体系位于波长652nm处的吸光度值降低;当待测体系中存在Hg~(2+)时,ssDNA上的胸腺嘧啶碱基(T)与Hg~(2+)发生特异性结合作用,形成T-Hg~(2+) -T类似双链结构的稳定复合物,该复合物与GO的作用力较弱,不易吸附于其表面,因此不影响GO的模拟酶活性,体系吸光度值增强。在一定条件下Hg~(2+)浓度越大,覆盖在GO表面的ssDNA越少,体系吸光度越强,据此建立检测Hg~(2+)的新方法。当汞离子浓度在3.26×10~(-8)~9.0×10~(-7) mol·L~(-1)范围内时,体系的ΔA值与汞离子浓度呈现良好的线性关系。其线性方程为ΔA=41.75c(nmol· L~(-1))+0.048 7,相关系数r=0.997 3,检出限为9.79×10~(-9) mol·L~(-1)。该方法简单、直观,抗干扰能力强、无需昂贵仪器设备,可用于检测环境水样中Hg~(2+)的含量。
语种：
中文
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Novel and label-free colorimetric detection of radon using AuNPs and lead(II)-induced GR5 DNAzyme-based amplification strategy
作者：
Liu, Hongwen;Chen, Yating;Song, Chunli;Tian, Gang;Li, Shiya;...
期刊：
Analytical and Bioanalytical Chemistry ,2018年410(17):4227-4234 ISSN：1618-2642
通讯作者：
Lv, Changyin
作者机构：
[Chen, Yating; Li, Shiya; Yang, Guiying; Lv, Changyin; Liu, Hongwen; Song, Chunli; Tian, Gang] Univ South China, Coll Publ Hlth, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Lv, Changyin] U;Univ South China, Coll Publ Hlth, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
DNAzyme;Gold nanoparticles;Lead;Radon;Spectrophotometry
摘要：
Radioactive radon decays into a stable daughter product, 210Pb, which was used as the detection target to determine the radon radiation dose in a new technique. Pb2+ triggers DNAzyme to cleave a molecular beacon (MB), resulting in the stem–loop structure opening and forming two single DNA strands (ssDNA). The ssDNA binds to unmodified gold nanoparticles and effectively prevents their aggregation in a salt solution. The detached enzyme strands continue to complement the remaining MB to amplify the response signal. The method proposed in this study exhibited a good linear relationship for Pb2+ and radon concentrations in the range of 6.22 × 102–1.02 × 105Bqh/m3 with a detection limit of 186.48Bqh/m3 using an ultraviolet–visible spectrometer. In practical applications, this sensitive method can avoid radioactive damage in field testing, and the detection limit meets the national standard in China. Importantly, this simple, highly sensitive strategy uses simple equipment and has a strong anti-interference ability. [Figure not available: see fulltext.]. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
语种：
英文
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Fluorescent resonance energy transfer of organic fluorescent dyes with gold nanoparticles and their analytical application
作者：
Zhou, Bin* ;Chen, Ya-Ting;Zhen, Xiao-Li;Lou, Liang;Wang, Yong-Sheng;...
期刊：
Gold Bulletin ,2018年51(4):145-151 ISSN：2364-821X
通讯作者：
Zhou, Bin
作者机构：
[Zhou, Bin; Suo, Qing-Li] Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan Childrens Hosp, Wuhan Maternal & Child Healthcare Hosp, Wuhan 430016, Hubei, Peoples R China.;[Lou, Liang; Zhen, Xiao-Li; Chen, Ya-Ting; Wang, Yong-Sheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Zhou, Bin] H;Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan Childrens Hosp, Wuhan Maternal & Child Healthcare Hosp, Wuhan 430016, Hubei, Peoples R China.
关键词：
Fluorescence resonance energy transfer;Fluorescence quenching;AuNPs;Mercury
摘要：
We report five effective fluorescence resonance energy transfer (FRET) systems based on gold nanoparticles (AuNPs) and organic fluorescent dyes, including ionic [fluorescein sodium (FS) and Eosin B (EB)] and cationic [rhodamine 6G (Rh6G), acridine orange (AO), and safranine T (ST)] fluorescent dyes. The fluorescence intensity of the five FRET systems demonstrates that efficient quenching is possible. The quenching efficiencies of Rh6G and FS by FRET were nearly 100%, 89% for AO, 60% for EB, and 55% for ST. A series of UV absorbance spectra and fluorescence emission spectra were used to explain the mechanism of fluorescence quenching. We found that there were different degrees of overlap between the absorption spectrum of the AuNPs and the emission spectrum of fluorescence dyes. This outcome indicates that highly efficient FRET is the possible mechanism of fluorescence quenching. We applied the FRET system to establish a sensitive and simple strategy for the determination of mercury (Hg2+). The maximum excitation was at 523nm (λex = 523nm). The enhanced fluorescence intensity at 551nm was proportional to the concentration of Hg2+ with a range of 0.44–100nmolL−1. The detection limit was 0.13nM. The linear regression equation was ΔF = 27.05c (nmolL−1) − 79.88, and the regression coefficient was 0.9954. The proposed method has high sensitivity and convenience and does not require complex and expensive instruments. © 2018, Springer International Publishing AG, part of Springer Nature.
语种：
英文
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A label-free ultrasensitive and selective strategy for Pb(ii) assay by a multifunctional DNA probe-mediated rolling-circle amplified synthesis of the G-quadruplexes
作者：
Wang, Xiao-Feng;Wang, Yong-Sheng* ;Xiao, Xi-Lin;Lan, Wen-Bo;Zhou, Bin;...
期刊：
ANALYTICAL METHODS ,2018年10(25):3081-3088 ISSN：1759-9660
通讯作者：
Wang, Yong-Sheng
作者机构：
[Zhou, Bin; Chen, Si-Han; Xue, Jin-Hua; Wang, Xiao-Feng; Wang, Yong-Sheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Lan, Wen-Bo; Wang, Xiao-Feng] Xiang Nan Univ, Coll Publ Hlth, Chenzhou 423000, Peoples R China.;[Xiao, Xi-Lin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
We report a label-free ultrasensitive and selective strategy for Pb(ii) assay by a multifunctional DNA probe (MDP)-mediated rolling-circle amplified synthesis of the G-quadruplexes. The MDP acted as a target recognition probe, a catalytic DNAzyme and a primer of rolling-circle amplification (RCA). The presence of Pb(ii) can induce the conformational switching of the MDP, resulting in the cleavage of the S-DNA in the MDP to release an E-DNA. The released E-DNA then initiated a RCA reaction with a reasonably devised padlock DNA template to produce an accumulated amount of repeated sequences. The RCA product in the present work was designed as a G-rich sequence, which could fold into thousands of G-quadruplex units. The G-quadruplex formed by RCA can specifically bind to NMM to result in an amplified fluorescence signal. Through these cascade amplifications, Pb(ii) ions can be detected at as low as 94.29 pM, which is much lower than those reported in related literature. We expect that this amplification strategy might be helpful in the design of a highly sensitive analytical platform for wide application in environmental and biomedical fields. © The Royal Society of Chemistry.
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英文
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