Genotoxicity of the sediments collected from Pearl River in China and their polycyclic aromatic hydrocarbons (PAHs) and heavy metals
作者：
Feng, Shaolong* ;Mai, Bixian;Wei, Gangjian;Wang, Xinming
期刊：
Environmental Monitoring and Assessment ,2012年184(9):5651-5661 ISSN：0167-6369
通讯作者：
Feng, Shaolong
作者机构：
[Feng, Shaolong] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Feng, Shaolong; Mai, Bixian; Wang, Xinming] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China.;[Wei, Gangjian] Chinese Acad Sci, Guangzhou Inst Geochem, Lab Isotope Geochronol & Geochem, Guangzhou 510640, Guangdong, Peoples R China.
通讯机构：
[Feng, Shaolong] U;Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Pearl River;Sediment;Micronucleus (MN) assay;Polycyclic aromatic hydrocarbons (PAHs);Heavy metals (HMs)
摘要：
The accelerated industrialization and urbanization in the last three decades around the Pearl River Delta within Guangdong Province in China have led to serious concerns about the impacts on the aquatic environment. In the present study, the genotoxicity of the sediments collected from the Pearl River was evaluated by micronucleus (MN) assay with Vicia faba root tip cells, and the 16 EPA priority polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs, including Cr, Cu, As, Se, Cd, Hg, and Pb) in the sediments were determined respectively by GC-MS, inductively coupled plasma mass spectrometry, and inductively coupled plasma atomic emission spectrometry. The results showed that there were significant increases of MN frequencies observed in the sediment-exposed groups, compared with the negative group (P<0.05, P<0.01), indicating that the sediments clearly had genotoxicity to the V. faba root cells. The total concentrations of the priority PAHs (250-13,656 ng g<sup>-1</sup>, dry weight) and HMs (As, 22,770-36,639 μg kg- 1;Cr, 39,333-133,343 μg kg<sup>-1</sup>;Cu, 36,145-159,270 μg kg<sup>-1</sup>;Pb, 51,210-166,642 μg kg<sup>-1</sup>;Cd, 475.4-1,818.9 μg kg <sup>-1</sup>;Hg, 59.9-460.8 μg kg<sup>-1</sup>;and Se, 331.7-1,250.4 μg kg<sup>-1</sup>, dry weight) were close to those obtained from other urbanized and industrialized areas, which have been considered moderately polluted. There was a clear positive correlation between MN potency and the molar concentrations of Hg and Pb in the sediments (Hg, r=0.94;Pb, r=0.91), suggesting that Hg and Pb were the most important factors that posed the sediments higher genotoxicity to V. faba root cells. Our results suggested that both biological and chemical approaches are necessary to be included in a battery of tests to assess the eco-environmental risks of sediments. ©Springer Science+Business Media B.V. 2011.
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英文
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液相链烷烃热导率与其结构定量关系
作者：
彭国文;肖方竹;聂长明;廖力夫;杨胜园;...
期刊：
化工学报 ,2011年62(3):604-610 ISSN：0438-1157
通讯作者：
Peng, G.(pgwnh@sohu.com)
作者机构：
[廖力夫; 聂长明; 肖锡林; 彭国文] School of Chemistry and Chemical Engineering, University South China, Hengyang 421001, Hunan, China;[杨胜园; 肖方竹] School of Public Health, University South China, Hengyang 421001, Hunan, China;[聂长明] Laboratory of Quantum Chemistry and Physics, University of Paul Sabatier(Toulouse-III), Toulouse 31077, France;[杨胜园] State Key Laboratory of Chemo-Biosensing and Chemometrics, Hunan University, Changsha 410082, Hunan, China
通讯机构：
School of Chemistry and Chemical Engineering, University South China, China
关键词：
液相链烷烃;热导率;平衡电负性;拓扑模型;定量关系
摘要：
应用密度泛函理论(DFT),在B3LYP/6-31+G(d)水平上对含有5～24个碳原子的部分液相链烷烃进行结构优化和量化计算其分子中各个原子间空间拓扑距离,并构建拓扑距离矩阵.结合分子中各原子的支化度,应用原子的平衡电负性对分子图进行着色,得到新型结构拓扑指数PX<,m>(m=1,2,3).采用多元线性回归技术建立液相链烷烃热导率γ与PX[m](m=1,2,3)的定量关系拓扑模型,并用该模型对热导率γ进行预测与估算,结果表明实验值与预测值、估算值均很吻合.同时,采用留一法(1eave-one-out)和外检验方法测试模型的内部稳定性和外部预测能力.与文献结果比较,本文所用参数少,计算简便,为液相链烷烃导热的研究提供了一种新方法.
语种：
中文
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量子拓扑指数法预测多氯联苯醚的热力学性质
作者：
肖方竹;彭国文;聂长明;杨胜园
期刊：
化工学报 ,2011年62(7):1808-1816 ISSN：0438-1157
通讯作者：
Peng, G.(pgwnh@sohu.com)
作者机构：
[聂长明; 肖方竹] School of Public Health, University South China, Hengyang 421001, Hunan, China;[杨胜园; 彭国文] School of Chemistry and Chemical Engineering, University South China, Hengyang 421001, Hunan, China;[聂长明] Laboratory of Quantum Chemistry and Physics, University of Paul Sabatier (Toulouse-III), Toulouse 31077, France;[杨胜园] State Key Laboratory of Chemo-Biosensing and Chemometrics, Hunan University, Changsha 410082, Hunan, China
通讯机构：
School of Chemistry and Chemical Engineering, University South China, China
关键词：
量子拓扑指数;多氯联苯醚;热力学性质;平衡电负性;拓扑模型;定量结构-性质相关
摘要：
应用密度泛函理论(DFT),在B3LYP/6-31+G(d)基组上优化和振动分析计算了多氯联苯醚(PCDEs)所有209种可能的分子空间几何结构,得到其各原子之间空间拓扑距离,并建立拓扑空间距离矩阵.结合分子中各原子的支化度,应用原子的平衡电负性对分子图进行着色,得到量子拓扑指数PX<,1>、PX<,2>.采用多元线性回归技术建证联苯醚和209种可能结构的多氯联苯醚PCDEs 3种少见报道的热力学性质--标准生成热、标准生成自由能和相对自由能与PX<,1>、PX<,2>的定量关系拓扑模型,并用该模型分别对不同热力学性质进行预测与估算.结果表明,本方法的预测值、估算值与文献[3]的计算值均相吻合.同时,采用留一法(leave-one-out)和外检验方法测试模型的内部稳定性和外部预测能力,测试结果显示模型具有良好的稳定性和较强的预测能力.
语种：
中文
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Determination of trace formaldehyde in blood plasma by resonance fluorescence technology
作者：
Wang, Yong-Sheng* ;Tan, Xuan;Xue, Jin-Hua;Li, Gui-Rong;Shi, Lin-Fei;...
期刊：
Analytica Chimica Acta ,2011年690(2):234-239 ISSN：0003-2670
通讯作者：
Wang, Yong-Sheng
作者机构：
[Zhou, Bin; Shi, Lin-Fei; Tan, Xuan; Li, Gui-Rong; Xue, Jin-Hua; Yang, Hong-Mei; Wang, Yong-Sheng; Liu, Lu] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Blood plasma;Formaldehyde;Potassium bromate;Pyronine Y;Resonance fluorescence
摘要：
A novel method for the determination of trace formaldehyde in blood plasma has been established by using resonance fluorimetry technique. It was based on the fact that oxidation of pyronine Y by potassium bromate was catalyzed by formaldehyde in sulfuric acid. When the wavelength interval was at Δλ=0nm, it was found that the decreased intensity (ΔF) of resonance fluorescence at 574.6nm was proportional to the concentration of formaldehyde in the range of 1.27×10-2 to 2.28μgmL-1. The limit of detection and the average recovery for formaldehyde were 3.80ngmL-1 and 101.6% (n=6), respectively. The present method had been applied to the determination of trace formaldehyde in blood plasma, and the obtained results were in good agreement with those obtained by the resonance light scattering method. © 2011 Elsevier B.V.
语种：
英文
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Simultaneous determination of norfloxacin and lomefloxacin in milk by first derivative synchronous fluorescence spectrometry using Al (III) as an enhancer
作者：
Yi, Yan-Ni;Li, Gui-Rong* ;Wang, Yong-Sheng;Zhou, Yu-Zhen;Zhu, Hui-Min
期刊：
Analytica Chimica Acta ,2011年707(1-2):128-134 ISSN：0003-2670
通讯作者：
Li, Gui-Rong
作者机构：
[Zhou, Yu-Zhen; Yi, Yan-Ni; Li, Gui-Rong; Zhu, Hui-Min; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Gui-Rong] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
First derivative spectrum;Lomefloxacin;Norfloxacin;Synchronous fluorimetry;Zero-crossing
摘要：
A novel method for the simultaneous determination of norfloxacin (NFLX) and lomefloxacin (LFLX) in milk samples was developed by using first derivative synchronous fluorimetry. The synchronous fluorescence (Δλ=160nm) spectra and first derivative synchronous fluorescence spectra of NFLX, LFLX and their mixture were studied. The zero-crossing method was utilized to measure the first derivative value of the derivative spectrum. The zero-crossing points were located at 275.0nm for NFLX and at 283.8nm for LFLX, in first derivative synchronous fluorescence spectra. Therefore, 283.8nm and 275.0nm were selected for the determination of NFLX and LFLX. The first derivative values varied linearly with the concentrations in the range of 1.68×10 -8-5.64×10 -6molL -1 for NFLX and 1.89×10 -8-6.19×10 -6molL -1 for LFLX. The detection limits were 5.03×10 -9molL -1 for NFLX and 7.58×10 -9molL -1 for LFLX. The proposed method is reliable, selective and sensitive, and has been used successfully in the simultaneous determination of NFLX and LFLX in milk samples, whose results were in good agreement with those obtained by HPLC. © 2011 Elsevier B.V.
语种：
英文
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A Wireless magnetoelastic sensor for urinary 2-naphthol detection based on the precipitation of 2-naphthol with diazonium salts
作者：
Yuan, Yu-Kun;Wang, Yong-Sheng* ;Xiao, Xi-Lin;Xue, Jin-Hua;Li, Gui-Rong;...
期刊：
Analytical Sciences ,2011年27(5):517-521 ISSN：0910-6340
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Shi, Lin-Fei; Kang, Rong-Hui; Li, Gui-Rong; Zhang, Jin-Quan; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Yuan, Yu-Kun] Chenzhou Fourth Peoples Hosp, Clin Lab, Chenzhou 423000, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
A wireless magnetoelastic sensor has been developed for the determination of 2-naphthol (2-NAP) in human urine. This method is based on the precipitation of 2-NAP with diazonium salts produced by the diazo-reaction of sulfamethoxazole (SMZ) with nitrite under a weak alkaline condition, resulting in a descending of the resonance frequency of a wireless magnetoelastic sensor. The frequence shift values (ΔF) of the sensor were directly proportional to the concentration in the range of 1.13 - 139 μmol L -1 for 2-NAP with a correlation coefficient of 0.997 and a detection limit of 0.340 μmol L -1 . The relative standard deviations were 2.38, 2.40 and 2.44%, and the average recovery was 107% (n = 6). The proposed method has additional advantages of being less time-consuming, low cost and remote query, and can be applied for realtime and in situ monitoring of 2-NAP in human urine. It would be a benefit to extend the scope of applications of magnetoelastic sensing techniques. 2011 © The Japan Society for Analytical Chemistry.
语种：
英文
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Detection of Mycoplasma genitalium using a wireless magnetoelastic immunosensor
作者：
He, Bo;Liao, Lifu* ;Xiao, Xilin;Gao, Shuqin;Wu, Yimou
期刊：
Analytical Biochemistry ,2011年408(1):1-4 ISSN：0003-2697
通讯作者：
Liao, Lifu
作者机构：
[Gao, Shuqin; Xiao, Xilin; Liao, Lifu; He, Bo] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[He, Bo] Univ S China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Xilin] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Wu, Yimou] Univ S China, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Immunosensor;Magnetoelastic;Mycoplasma genitalium;Wireless
摘要：
A wireless immunosensor for the detection of Mycoplasma genitalium was fabricated by immobilizing polyclonal antibody onto the surface of a magnetostrictive strip. In response to a time-varying magnetic field, the immunosensor longitudinally vibrates at a resonance frequency, emitting magnetic flux that can be remotely detected by a pickup coil. No physical connections between the immunosensor and the detection system are required, facilitating wireless aseptic operation. The binding of M. genitalium to the immunosensor surface resulted in a decrease in the resonance frequency of the immunosensor. When solutions with varying concentrations of the bacteria were tested, the shift of the resonance frequency was proportional to the concentration of M. genitalium. Under the optimized conditions, the linear range for the determination of M. genitalium was 2.0×103 to 2.9×104 color change units (ccu)/ml with a detection limit of 3.4×102 ccu/ml. The immunosensor was successfully applied to real samples containing M. genitalium with results similar to those previously obtained by the color change unit method. © 2010 Elsevier Inc.
语种：
英文
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Highly sensitive inhibitory kinetics fluorescence method for determination of arsenic
作者：
Li, Guirong* ;Liu, Yunfu
期刊：
International Journal of Environmental Analytical Chemistry ,2011年91(9):866-875 ISSN：0306-7319
通讯作者：
Li, Guirong
作者机构：
[Liu, Yunfu; Li, Guirong] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Guirong] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Acridine red;Arsenic;Food products;Inhibitory kinetics fluorescence
摘要：
A new and highly sensitive inhibitory kinetic fluorescence method for the determination of arsenic (III) has been established based on its inhibitory effect on the oxidation reaction of Acridine red (ADR) by KBrO<inf>3</inf> in sulphuric acid medium. The reaction has been followed by measuring the enhancement of fluorescence at 550 nm. It relies on the linear relationship where the change in the fluorescence (ΔF) versus added As(III) amounts in the range of 0-0.450 μgmL<sup>-1</sup> is plotted, under the optimum conditions. The sensitivity of the proposed method, i.e. the limit of detection, is 2.1 ×10<sup>-2</sup> ngmL<sup>-1</sup>. The method is featured with good accuracy and reproducibility for arsenic (III) determination. This method was successfully applied for the quantitative determination of arsenic (III) in food products samples, and the relative standard deviations and the recoveries were in ranges of 2.31-2.83% and 90.0-107.2%, respectively. A review of recently published catalytic or inhibiting kinetic methods for the determination of arsenic (III) has also been presented for comparison. The mechanism of reaction was studied. ©2011 Taylor &Francis.
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英文
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Simultaneous determination of a-naphthol, b-naphthol and 1-hydroxypyrene in urine by synchronous fluorescence spectrometry using b-cyclodextrin as a sensitiser
作者：
Wang, Ying;Wang, Yong-Sheng* ;Yang, Hong-Mei;Xue, Jin-Hua;Tan, Xuan;...
期刊：
International Journal of Environmental Analytical Chemistry ,2011年91(1):87-96 ISSN：0306-7319
通讯作者：
Wang, Yong-Sheng
作者机构：
[Guo, Yu; Wang, Ying; Tan, Xuan; Yang, Hong-Mei; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Ying] Hengyand Blood Ctr, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
alpha-naphthol;beta-naphthol;1-hydroxypyrene;synchronous fluorescence;beta-cyclodextrin;double scans;urine
摘要：
A novel method for the simultaneous determination of alpha-naphthol (alpha-NAP), beta-naphthol (beta-NAP) and 1-hydroxypyrene (1-OHP) in human urine has been established by using synchronous fluorescence spectrometry. The measurement was carried out in sodium acetate-boroborax buffer solution (pH = 5.0) with beta-cyclodextrin (beta-CD) enhancing fluorescence. At D Delta lambda = 23 nm, 1-OHP and beta-NAP exhibit maximum signal with minimum interferences from alpha-NAP. At Delta lambda = 175 nm, the signal of alpha-NAP is not influenced by the presence of 1-OHP and beta-NAP. The signals detected at these three wavelengths, 360.2 nm, 330.6nm and 296.4nm, vary linearly when the concentration of 1-OHP, beta-NAP and alpha-NAP is in the range of 0.65-218.3 ng mL(-1), 2.8-1441.0 ng mL(-1) and 3.6-1586.0 ng mL(-1), respectively. The limits of detection (LOD) for alpha-NAP, beta-NAP and 1-OHP were 1.53 ng mL(-1), 0.78 ng mL(-1) and 0.020 ng mL(-1) with relative standard deviations (RSD) of 2.3%, 2.4% and 1.8%, respectively. The proposed method was successfully applied for the simultaneous determination of alpha-NAP, beta-NAP and 1-OHP in human urine samples, and the obtained results were in good agreement with those obtained by the method of high-performance liquid chromatography (HPLC).
语种：
英文
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叶顶单一气膜孔位置传热特性的数值研究
作者：
何博;廖力夫;胡狮进;高淑琴;肖锡林
期刊：
工程热物理学报 ,2011年32(10):1761-1764 ISSN：0253-231X
通讯作者：
Wang, J.
作者机构：
[何博; 廖力夫] 南华大学公共卫生学院;[胡狮进; 高淑琴; 肖锡林] 南华大学化学化工学院
通讯机构：
Key Laboratory of Thermo-Fluid Science and Engineering of MOE, Xi'an Jiaotong University, China
关键词：
叶片;气膜冷却;冷却效率;传热系数
摘要：
本文采用数值模拟的方法对凹槽叶顶前缘气膜孔的位置进行了研究,通过改变前缘第一气膜孔位置来分析叶顶区传热。数值模拟结果表明,由于冷却气对该区域没有提供足够的保护,肩壁表面的平均绝热效率远低于凹槽底面,平均传热系数远高于凹槽底面。叶顶第一气膜孔向前缘移动使肩壁表面的平均传热系数先升高再降低,叶顶前缘低绝热冷却效率区逐渐消失。
语种：
中文
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Determination of urinary adenosine using resonance light scattering of gold nanoparticles modified structure-switching aptamer
作者：
Zhang, Jin-Quan;Wang, Yong-Sheng* ;He, Yan;Jiang, Tao;Yang, Hong-Mei;...
期刊：
Analytical Biochemistry ,2010年397(2):212-217 ISSN：0003-2697
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Shi, Lin-Fei; Kang, Rong-Hui; Tan, Xuan; Zhang, Jin-Quan; Yang, Hong-Mei; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[He, Yan; Jiang, Tao] Univ S China, Coll Life Sci & Technol, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Adenosine;Aptamer;G-quartet;Gold nanoparticle;Resonance light scattering
摘要：
A novel sensitive method has been developed for the detection of adenosine (AD) in human urine by using enhanced resonance light scattering (RLS). This method is based on the specific recognition and signal amplification of adenosine aptamer (Apt) coupled with gold nanoparticles (GNPs) via G-quartet-induced nanoparticle assembly, which was fabricated by triggering a structure switching of the 3′ terminus G-rich sequence and aptamer duplex. RLS signal linearly correlated with the concentration of adenosine over the range of 6-115 nM. The limit of detection (LOD) for adenosine is 1.8 nM with relative standard deviations (RSD) of 2.90-4.80% (n = 6). The present method has been successfully applied to determination of adenosine in real human urine, and the obtained results were in good agreement with those obtained by the HPLC method. Our investigation shows that the combination of the excellent selectivity of aptamer with the high sensitivity of the RLS technique could provide a promising potential for aptamer-based small molecule detection, and be beneficial in extending the application of RLS. © 2009 Elsevier Inc. All rights reserved.
语种：
英文
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TiO2 Nanoparticles Co-Doped with Silver and Nitrogen for Antibacterial Application
作者：
Yuan, Yali* ;Ding, Jianqiang;Xu, Jinsheng;Deng, Jian;Guo, Jianbo
期刊：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY ,2010年10(8):4868-4874 ISSN：1533-4880
通讯作者：
Yuan, Yali
作者机构：
[Yuan, Yali; Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Ding, Jianqiang] Zhenhai Ctr Dis Control & Prevent, Ningbo 315200, Peoples R China.;[Xu, Jinsheng] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.;[Guo, Jianbo] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Yuan, Yali] U;Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Antibacterial property;Co-doped;Nanoparticle;TiO 2
摘要：
We have prepared a series of TiO<inf>2</inf> nanoparticles for antibacterial applications. These TiO<inf>2</inf> nanoparticles were prepared by the hydrolysis precipitation method with Ti(OBu)<inf>4</inf>, silver nitrate and ammonia. Crystal structure, particle size, interfacial structure and UV-visible light response of the prepared nanoparticles were characterized by X-ray diffraction measurements (XRD), Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR) and UV-Vis diffuse reflectance spectroscopy (UV-Vis-DRs). The XRD spectra showed that all samples were anatase structure calcined at 450 °C for 3 hours. The Ag doping made the peak of diffraction wider. The results of TEM showed that the nanoparticles of TiO <inf>2</inf>, N-TiO<inf>2</inf> and 1% Ag-N-TiO<inf>2</inf> were all spherical in shape and well distributed with a mean size of 19.8 nm, 39.2 nm and 20.7 nm, respectively. N doping caused the nanoparticle size to increase, while, when the doped amount of Ag<sup>+</sup> increased, the TiO<inf>2</inf> particle size decreased. The FTIR revealed that Ag and N doping of TiO<inf>2</inf> appeared to have strong absorption by -OH group and showed the characteristic absorption band of NH<sup>+</sup><inf>4</inf> and Ag. The UV-Vis-DRs indicated that the absorption band of Ag-N co-doped TiO<inf>2</inf> had red shift and that the optical absorption response (between 400 nm and 700 nm) had obvious enhancement. The antibacterial properties of nanoparticles were investigated by agar diffusion method toward Escherichia coli and Bacillus subtilis. The results indicated that both Ag-and N-doped TiO<inf>2</inf> could increase the antibacterial properties of TiO<inf>2</inf> nanoparticles under fluorescent light irradiation. A 1% Ag-N-TiO<inf>2</inf> had the highest antibacterial activity with a clear antibacterial circle of 33.0 mm toward Escherichia coli and 22.8 mm toward Bacillus subtilis after cultivation for 24 hours. Copyright ©2010 American Scientific Publishers.
语种：
英文
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Simultaneous spectrophotometric determination of trace amount of malachite green and crystal violet in water after cloud point extraction using partial least squares regression
作者：
An, Lin;Deng, Jian* ;Zhou, Liang;Li, Hui;Chen, Fei;...
期刊：
Journal of Hazardous Materials ,2010年175(1-3):883-888 ISSN：0304-3894
通讯作者：
Deng, Jian
作者机构：
[Liu, Yating; Chen, Fei; An, Lin; Wang, Hui; Li, Hui; Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Zhou, Liang] Univ S China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Cloud point extraction;Crystal violet;Malachite green;Partial least squares regression;Spectrophotometry
摘要：
In this work, a new method has been proposed to simultaneously determine the trace amount of malachite green and crystal violet from aqueous solution by spectrophotometry after cloud point extraction (CPE) using partial least squares regression. The optimal extraction and operating conditions, such as pH, reagents concentration and effect of time and temperature, and so on, have been investigated using the non-ionic surfactant Triton X-114. The maximum absorption wavelength for malachite green and crystal violet is 624 and 579 nm, respectively;linearity is obeyed in the range of 9.9-800 and 16-1000 ng mL<sup>-1</sup> with detection limit of 2.9 and 4.8 ng mL<sup>-1</sup>, and the root mean square error of prediction (RMSEP) are 0.0197 and 0.0343, respectively. The proposed method has been applied successfully to simultaneously determine the trace amount of malachite green and crystal violet in real matrix samples with the recoveries of 92.45-102.5%. ©2009 Elsevier B.V. All rights reserved.
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Quartz crystal microbalance with β-cyclodextrin/TiO2 composite films coupled with chemometrics for the simultaneous determination of urinary 1- and 2-naphthol
作者：
Yuan, Yu-Kun;Xiao, Xi-Lin;Wang, Yong-Sheng* ;Xue, Jin-Hua;Li, Gui-Rong;...
期刊：
Sensors and Actuators B-Chemical ,2010年145(1):348-354 ISSN：0925-4005
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Shi, Lin-Fei; Kang, Rong-Hui; Li, Gui-Rong; Zhang, Jin-Quan; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, W Changsheng Rd 28, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, W Changsheng Rd 28, Hengyang 421001, Peoples R China.
关键词：
Naphthol;beta-Cyclodextrin;TiO2;Quartz crystal microbalance;Chemometrics
摘要：
A novel sensitive method was presented for the simultaneous determination of 1-naphthol (1-NAP) and 2-naphthol (2-NAP) in human urine samples. This method is based on the combination of the high sensitivity of quartz crystal microbalance (QCM) and excellent predictive ability of chemometrics. Nanocrystalline TiO<inf>2</inf> films were used as substrates for immobilizing β-cyclodextrin (β-CD) onto the QCM surface. The sensing layer consisting of β-CD and nanocrystalline TiO<inf>2</inf> particles was characterized by X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TG) and IR spectroscopy. In the Britton-Robinson (B-R) buffer solution of pH 6.37, frequency shift (ΔF) of the QCM was directly proportional to the concentration of 1-NAP and 2-NAP as 1.0 ×10<sup>-6</sup> to 1.3 ×10<sup>-4</sup> mol L<sup>-1</sup>, and 1.0 ×10<sup>-6</sup> to 1.2 ×10<sup>-4</sup> mol L<sup>-1</sup>, respectively. The correlation coefficients were 0.993 for 1-NAP, and 0.990 for 2-NAP. The limits of detection (LOD) were found to be 8.362 ×10<sup>-7</sup> mol L<sup>-1</sup> for 1-NAP and 2.146 ×10<sup>-7</sup> mol L<sup>-1</sup> for 2-NAP. The proposed method has been successfully applied for detecting 1-NAP and 2-NAP in human urine with a satisfactory result. The QCM with β-CD/TiO<inf>2</inf> composite films coupled with chemometrics can lead to significant improvements in the sensor performance, and would be benefit to extend the scope of application of QCM and chemometrics. ©2009 Elsevier B.V. All rights reserved.
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Study on the enhanced fluorescent spectrum of ciprofloxacin + Al(III) + La(III) + cetyltrimethylammonium bromide system and its application
作者：
Wei, Leilei;Li, Guirong* ;Li, Haipeng
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2010年75(5):1486-1491 ISSN：1386-1425
通讯作者：
Li, Guirong
作者机构：
[Wei, Leilei; Li, Haipeng; Li, Guirong] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Guirong] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Ciprofloxacin;Aluminum;Lanthanum;Fluorescence method;Co-luminescence
摘要：
The fluorescence of ciprofloxacin (CIP) in HAc-NaAc buffer solution and the presence of cetyltrimethylammonium bromide (CTMAB) enhanced visibly with adding Al(III) and La(III). This enhanced fluorescence spectra were studied, and a new co-luminescence system of CIP + Al(III) + La(III) + CTMAB was discovered. There was a linear relationship between the enhanced fluorescence intensity and the concentration of CIP in the range of 0.50-80.2 μg l<sup>-1</sup> under the optimized condition. A novel enhanced fluorescence method for the determination of trace CIP was established by using this co-luminescence system. The detection limit of the proposed method was 0.17 μg l<sup>-1</sup> for CIP. This method is simple, rapid and sensitive. The CIP in milk samples were analyzed by the proposed method with satisfactory results. The relative standard deviation and the recovery were in ranges of 3.21-4.34% and 97.1-100.1%, respectively. The mechanism of the co-luminescence reaction and the reasons for fluorescence enhancement has been discussed. ©2010 Elsevier B.V. All rights reserved.
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Rapid simultaneous analysis of 1-hydroxypyrene, 2-hydroxyfluorene, 9-hydroxyphenanthrene, 1- and 2-naphthol in urine by first derivative synchronous fluorescence spectrometry using Tween-20 as a sensitizer
作者：
Kang, Rong-Hui;Wang, Yong-Sheng* ;Yang, Hong-Mei;Li, Gui-Rong;Tan, Man;...
期刊：
Analytica Chimica Acta ,2010年658(2):180-186 ISSN：0003-2670
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Tan, Man; Shi, Lin-Fei; Kang, Rong-Hui; Li, Gui-Rong; Zhang, Jin-Quan; Yang, Hong-Mei; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Derivative synchronous fluorescence;Polycyclic aromatic hydrocarbons;Tween-20;Urine
摘要：
A novel method of first derivative synchronous fluorescence was developed for the rapid simultaneous analysis of trace 1-hydroxypyrene (1-OHP), 1-naphthol (1-NAP), 2-naphthol (2-NAP), 9-hydroxyphenanthrene (9-OHPe) and 2-hydroxyfluorene (2-OHFlu) in human urine. Only one single scan was needed for quantitative determination of five compounds simultaneously when Δλ = 10 nm was chosen. In the optimal experimental conditions, there was a linear relationship between the fluorescence intensity and the concentration of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in the range of 1.75 × 10-9 to 4.50 × 10-6 mol L-1, 3.64 × 10-8 to 2.20 × 10-4 mol L-1, 8.18 × 10-9 to 1.20 × 10-4 mol L-1, 3.26 × 10-9 to 8.50 × 10-5 mol L-1 and 4.88 × 10-9 to 5.50 × 10-6 mol L-1, respectively. The limits of detection (LOD) were found to be 5.25 × 10-10 mol L-1 for 1-OHP, 1.10 × 10-8 mol L-1 for 1-NAP, 2.46 × 10-9 mol L-1 for 2-NAP, 9.77 × 10-10 mol L-1 for 9-OHPe and 1.46 × 10-9 mol L-1 for 2-OHFlu. The proposed method is reliable, selective and sensitive, and has been used successfully in the determination of traces of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in human urine samples, whose results were in good agreement with those gained by the HPLC method. © 2009 Elsevier B.V. All rights reserved.
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英文
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A catalytic resonance fluorometry method for determination of hydroquinone and its applications
作者：
Li, Guirong* ;Tan, Guanghui;Liu, Yunfu;Wang, Yongsheng;Liao, Lifu
期刊：
Analytical Sciences ,2009年25(4):493-497 ISSN：0910-6340
通讯作者：
Li, Guirong
作者机构：
[Wang, Yongsheng; Tan, Guanghui; Liu, Yunfu; Li, Guirong] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Liao, Lifu] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Guirong] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
Pyronin Y (PY) has a strong resonance fluorescence in sulfuric acid medium. The characteristics of the resonance fluorescence spectra and the factors affecting the spectra were studied. A catalytic resonance fluorometry method for the determination of hydroquinone was proposed based on the catalytic effect of hydroquinone on the oxidation of PY by potassium bromate. The oxidation of PY resulted in the decrease of the resonance fluorescence intensity. The influences of several variables on the sensitivity were studied. At the optimized conditions, the decrease of the resonance fluorescence intensity was in proportion to the concentration of hydroquinone in the range of 4.42 - 1.60 × 103 μg l-1, and the detection limit was 1.46 μg l-1. The proposed method was applied successfully for the determination of trace hydroquinone in environment samples. The relative standard deviation was less than 3.90% and the recoveries were in the range of 95.2 - 104.0%. 2009 © The Japan Society for Analytical Chemistry.
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英文
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Monitoring of Mycoplasma genitalium growth and evaluation of antibacterial activity of antibiotics tetracycline and levofloxacin using a wireless magnetoelastic sensor
作者：
He, Bo;Liao, Lifu* ;Xiao, Xilin;Gao, Shuqin;Wu, Yimou
期刊：
Biosensors and Bioelectronics ,2009年24(7):1990-1994 ISSN：0956-5663
通讯作者：
Liao, Lifu
作者机构：
[Gao, Shuqin; Xiao, Xilin; Liao, Lifu; He, Bo] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Liao, Lifu; He, Bo] Univ S China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Xilin] Hunan Univ, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Wu, Yimou] Univ S China, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.;[Liao, Lifu] Univ S China, Coll Chem & Chem Engn, Xueyan Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ S China, Coll Chem & Chem Engn, Xueyan Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Magnetoelastic sensor;Mycoplasma genitalium;Wireless;Tetracycline;Levofloxacin
摘要：
Mycoplasma genitalium (Mg) is the smallest and simplest self-replicating bacteria lacking of cell wall and is a human pathogen causing various diseases. This paper describes the real-time, long-term and in situ monitoring of the growth of Mg and evaluation of the effect of the antibiotics tetracycline and levofloxacin on the growth using a wireless magnetoelastic sensor. The sensor is fabricated by coating a magnetoelastic strip with a polyurethane protecting film. In response to a time-varying magnetic field, the sensor longitudinally vibrates at a resonance frequency, emitting magnetic flux that can be remotely detected by a pick-up coil. No physical connections between the sensor and the detection system are required. The wireless property facilitates aseptic operation. The adhesion of Mg on the sensor surface results in a decrease in the resonance frequency, which is proportional to the concentration of Mg. The shift of the resonance frequency-time curves shows that under routine culture condition the growth curve of Mg is composed of three phases those are lag, logarithmic and stationary phase, respectively. In the presence of the antibiotics, the lag phase in the growth inhibition curves is prolonged obviously and the stationary phase is substituted by a decline phase. The growth inhibition of Mg is related to the concentration of the antibiotics. The MIC50 (minimal inhibitory concentration) of Mg incubated in the presence of the antibiotics for 120 h is calculated to be 1.5 and 0.5 μg/mL for tetracycline and levofloxacin, respectively. © 2008 Elsevier B.V. All rights reserved.
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英文
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The molecular resonance fluorescence method for determination of arsenic in hair samples
作者：
Li, Guirong* ;Liu, Yunfu;Wang, Yongsheng;Liao, Lifu
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2009年72(4):811-815 ISSN：1386-1425
通讯作者：
Li, Guirong
作者机构：
[Wang, Yongsheng; Liu, Yunfu; Li, Guirong] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Liao, Lifu] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Guirong] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Molecular resonance fluorescence;Arsenic;Rhodamine B;Hair samples
摘要：
A molecular resonance fluorescence quenching method for the determination of arsenic was proposed. The method is based on the quenching effect of As (V) on the molecular resonance fluorescence of Rhodamine B (RhB) in sulfuric acid medium and in the presence of molybdate and PVA. The influences of acidity, chemical and manifold variables on the sensitivity were studied. At the optimized conditions, the linear range for the determination of arsenic was 2.0 ×10<sup>-3</sup>-0.12 μg ml<sup>-1</sup> with the detection limit of 1.20 ×10<sup>-9</sup> g ml<sup>-1</sup>. The relative standard deviations for the eleven replicate determinations of 0.006 μg ml<sup>-1</sup> and 0.060 μg ml<sup>-1</sup> of arsenic were 1.12% and 1.61%, respectively. The proposed method was applied successfully for the determination of trace arsenic in hair samples with the recoveries of 94.8-104.6%. ©2008 Elsevier B.V. All rights reserved.
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英文
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TiO2 Nanopowder Co-Doped with Iodine and Boron to Enhance Visible-Light Photocatalytic Activity
作者：
Ding, Jianqiang;Yuan, Yali* ;Xu, Jinsheng;Deng, Jian;Guo, Jianbo
期刊：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY ,2009年5(5):521-527 ISSN：1550-7033
通讯作者：
Yuan, Yali
作者机构：
[Ding, Jianqiang; Guo, Jianbo] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Yuan, Yali; Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Xu, Jinsheng] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.
通讯机构：
[Yuan, Yali] U;Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Boron;Iodine;Methyl orange;Photocatalytic activity;TiO 2;Visible-light
摘要：
An iodine and boron co-doped TiO<inf>2</inf> photocatalyst was prepared by the hydrolyzation-precipitation method. X-ray diffraction (XRD), ultraviolet-visible diffuse reflectance spectroscopy (UV-Vis DRS), and X-ray photoelectron spectroscopy (XPS) were applied to characterize the crystalline structure, light absorbing ability, and the chemical state of iodine and boron in the photocatalysts. The results of photocatalytic degradation of methyl orange demonstrated that the I-B-TiO<inf>2</inf> catalyst prepared at 400 °C for 3 h exhibited the highest photocatalytic activity with a methyl orange degradation ratio of 61% under visible-light (λ&le 420 nm) irradiation for 120 min. The characterization results revealed that I-B-TiO<inf>2</inf> is in conformity with the anatase TiO<inf>2</inf> and that the doping of iodine and boron ions could efficiently inhibit the grain growth. Doped iodine was present in the multivalent forms of I<sup>7+</sup>, I<sup>-</sup> and I<sup>5+</sup>. Doped boron was present as B<sup>3+</sup> in an as-prepared sample, forming a possible chemical environment such as B-O-Ti. Overall, the doping of I and B enhanced the ability of TiO<inf>2</inf> to absorb visible-light, and it was observed that the photocatalytic activity of I-B-TiO<inf>2</inf> was enhanced by the synergistic effect of I and B. Copyright ©2009 American Scientific Publishers All rights reserved.
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