A novel method for the simultaneous determination of alpha-naphthol (alpha-NAP), beta-naphthol (beta-NAP) and 1-hydroxypyrene (1-OHP) in human urine has been established by using synchronous fluorescence spectrometry. The measurement was carried out in sodium acetate-boroborax buffer solution (pH = 5.0) with beta-cyclodextrin (beta-CD) enhancing fluorescence. At D Delta lambda = 23 nm, 1-OHP and beta-NAP exhibit maximum signal with minimum interferences from alpha-NAP. At Delta lambda = 175 nm, the signal of alpha-NAP is not influenced by the presence of 1-OHP and beta-NAP. The signals detecte...
