期刊论文

Wang, Yong-Sheng

英文

Analytical Biochemistry

0003-2697

2010

397

2

212-217

10.1016/j.ab.2009.10.027

The authors gratefully acknowledge the support of the Natural Science Foundation of Hunan Province, China (No. 03JJY3030 ), the Science and Technology Program of Hunan Province in China (No. 2008FJ3083 ), and the Science Research Program of Educational Department of Hunan Province in China (No. 08C718 ).

本校为第一且通讯机构

A novel sensitive method has been developed for the detection of adenosine (AD) in human urine by using enhanced resonance light scattering (RLS). This method is based on the specific recognition and signal amplification of adenosine aptamer (Apt) coupled with gold nanoparticles (GNPs) via G-quartet-induced nanoparticle assembly, which was fabricated by triggering a structure switching of the 3′ terminus G-rich sequence and aptamer duplex. RLS signal linearly correlated with the concentration of adenosine over the range of 6-115 nM. The limit ...