关键词：
small nucleolar RNA;SNORD16;colon cancer;oncogene;prognostic biomarker
摘要：
Colon cancer (CC) is considered one of the most common and lethal malignancies occurring both in male and female. Its widespread prevalence demonstrates the need for novel diagnostic and prognostic biomarkers for CC. Emerging evidence has shown that small nucleolar RNAs play critical roles in tumor development. In this study, we investigated the expression profile and functions of SNORD16 in CC. Our data showed that SNORD16, rather than its host gene (RPL4), was upregulated in CC cell lines. Compared to matched adjacent normal tissues, CC tissues showed higher SNORD16 expression levels, and no correlation was found between SNORD16 and RPL4. Patients with high SNORD16 expression levels had a worse prognosis, and multivariate analysis showed the high SNORD16 expression was an independent prognostic factor for CC. In vitro gain- and loss-of-function studies revealed that SNORD16 can promote cell growth, proliferation, migration, and invasion of CC cells by inhibiting apoptosis. These results suggested that SNORD16 has an oncogenic role in CC and might be a novel diagnostic and prognostic biomarker for CC.
期刊：
Research in Microbiology,2020年171(2):45-54 ISSN：0923-2508
通讯作者：
He, Shu-ya
作者机构：
[He, Shu-ya; Wang, Wu-zhou; He, Jun-yan; Ma, Yun; Qi, Hui-zhou] Univ South China, Hengyang Med Coll, Inst Biochem & Mol Biol, Hengyang 421001, Peoples R China.;[Qi, Hui-zhou] Univ South China, Hengyang Med Coll, Funct Lab Ctr, Hengyang 421001, Peoples R China.;[He, Shu-ya; Wang, Wu-zhou; He, Jun-yan; Xiao, Fang-zhu; Qi, Hui-zhou] Univ South China, Hengyang Key Lab Biol Effects Nucl Radiat, Hengyang 421001, Peoples R China.
通讯机构：
[He, Shu-ya] U;Univ South China, Hengyang Key Lab Biol Effects Nucl Radiat, Hengyang 421001, Peoples R China.
关键词：
Antioxidative system;Deinococcus radiodurans;Manganese;PprI;PprM;Reactive oxygen species
摘要：
Deinococcus radiodurans is famous for its extreme resistance to various stresses such as ionizing radiation (IR), desiccation and oxidative stress. The underlying mechanism of exceptional resistance of this robust bacterium still remained unclear. However, the antioxidative system of D. radiodurans has been considered to be the determinant factor for its unparalleled resistance and protects the proteome during stress, then the DNA repair system and metabolic system exert their functions to restore the cell to normal physiological state. The antioxidative system not only equipped with the common reactive oxygen species (ROS) scavenging enzymes (e.g. catalase and superoxide dismutase) but also armed with a variety of non-enzyme antioxidants (e.g. carotenoids and manganese species). And the small manganese complexes play an important role in the antioxidative system of D. radiodurans. Recent studies have characterized several regulators (e.g., PprI and PprM) in D. radiodurans, which play critical roles in the protection of the bacteria from various stresses. In this review, we offer a panorama of the progress regarding the characteristics of the antioxidative system in D. radiodurans and its application in the future.
作者机构：
[刘政海; 李彩; 王五洲; 曹文宇; 罗诗诗] Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang, 421001, China;[徐杨] Department of Physiology, Medical College, University of South China, Hengyang, 421001, China;[何洁] Department of Pathology, Medical College, University of South China, Hengyang, 421001, China;[Wang W.; 何淑雅] School of Public Health, University of South China, Hengyang, 421001, China;[艾小红] Department of Radiation Oncology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China
通讯机构：
[Cao, W.] C;Clinical Anatomy & Reproductive Medicine Application Institute, China
摘要：
<jats:sec><jats:title>Background</jats:title><jats:p>The aim of this study was to determine whether progesterone could inhibit the growth of lung adenocarcinoma cells via membrane progesterone receptor alpha (mPRα) and elucidate its potential mechanism. The relationship between mPRα expression and the survival prognosis of lung adenocarcinoma patients was studied.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>A mPRα knockdown lung adenocarcinoma cell line was constructed and treated with P4 and Org (a derivative of P4 and specific agonist of mPRα). Cell proliferation was assessed using CCK‐8 and plate colony formation assays. Protein expression was detected by western blotting. A nude mouse model of lung adenocarcinoma was established to assess the antitumor effect of P4/Org in vivo.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>We initially determined that mPRα could promote the development of lung adenocarcinoma through the following lines of evidence. High expression of mPRα both at the mRNA and protein level was significantly associated with the poor prognosis of lung adenocarcinoma patients. The downregulation of mPRα inhibited the proliferation of lung adenocarcinoma cells. We further showed that mPRα mediates the ability of P4 to inhibit the growth of lung adenocarcinoma cells through the following lines of evidence: P4/Org inhibited the proliferation of lung adenocarcinoma cells; mPRα mediated the ability of P4/Org to inhibit lung adenocarcinoma cell proliferation; mPRα mediated the ability of P4/Org to inhibit the PKA (cAMP‐dependent protein kinase)/CREB (cAMP responsive element binding protein) and PKA/β‐catenin signaling pathways; and P4/Org inhibited the growth of a lung adenocarcinoma tumor model in vivo.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In summary, the results of our study show that progesterone can inhibit lung adenocarcinoma cell growth via mPRα.</jats:p></jats:sec>
通讯机构：
[He, Shu-Ya; Peng, Guo-Wen] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.;Univ South China, Hunan Prov Engn Technol Res Ctr Uranium Tailings, Hengyang 421001, Peoples R China.
关键词：
calixarene;Fe 3 O 4;magnetic materials;separation.
摘要：
<jats:p>
Environmental contextUranium-containing wastewaters have high potential to harm the environment and human health. We found that the combination of calix[4]arene with magnetic Fe3O4 particles produced good adsorption of uranium from wastewater. In addition, this material can be recycled and reused, so it has good prospects in practical applications for uranium remediation.
AbstractA magnetic functionalised calix[4]arene composite consisting of Fe3O4 and calix[4]arene phosphonate derivative (CPD) was prepared through a facile self-assembly method. The composite was characterised by scanning electron microscopy (SEM), powder X-ray diffraction (PXRD) and Fourier transform infrared spectroscopy (FTIR). The as-synthesised Fe3O4/CPD composite was used to remove UVI from aqueous solutions under different conditions. Meanwhile, the adsorption isotherm, kinetics and thermodynamics were fitted and analysed. The results show that the Fe3O4/CPD composite may be a promising adsorption material for the separation and enrichment of UVI from aqueous solutions in the cleanup of environmental pollution.
</jats:p>
摘要：
Deinococcus radiodurans is a model microorganism used for studies on DNA repair and antioxidation due to its extraordinary tolerance to ionizing radiation and other DNA-damaging agents. Various transcriptome analyses have revealed that hundreds of genes are induced and that many other genes are repressed during recovery of D. radiodurans following irradiation, suggesting that gene regulation is of great importance for the extreme resistance of this microorganism to ionizing radiation. In this article, we focus on some reported strategies that are employed by D. radiodurans to regulate the genes implicated in its extreme tolerance to ionizing radiation for a comprehensive understanding of the reasons this bacterium can survive such extraordinary stress. We expect this review to shed light on potential radioprotective agents and applications for use in a range of fields.
作者机构：
[牛磊; 刘政海; 万炜; 曹文宇; 罗诗诗; 王贞] Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang, 421001, China;[乃爱桃] Department of Radiation Oncology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China;[徐杨] Department of Physiology, Medical College, University of South China, Hengyang, 421001, China;[何洁] Department of Pathology, Medical College, University of South China, Hengyang, 421001, China;[何淑雅] Department of Radiation Medicine, School of Public Health, University of South China, Hengyang, 421001, China
通讯机构：
[Wan, W.] C;Clinical Anatomy & Reproductive Medicine Application Institute, China
关键词：
电离辐射;海马;星形胶质细胞;认知功能障碍
摘要：
探讨电离辐射引起小鼠认知功能障碍的可能机制。24只雌性昆明小鼠随机分为对照组和辐照组,辐照组小鼠接受137Csγ射线单次全身辐照至吸收剂量4 Gy。35 d后采用Y迷宫实验检测小鼠认知功能;免疫组织化学法检测小鼠海马区星形胶质细胞(Astrocytes,Ast)标记物胶质纤维酸性蛋白(Glial fibrillary acidic protein,GFAP)阳性细胞数的表达变化;Western blot方法检测海马GFAP蛋白的表达变化;荧光定量PCR检测炎性因子白细胞介素IL-1α、IL-1β、IL-6 mRNA和海马GFAP的表达情况。结果表明:与对照组相比,辐照组小鼠在Y迷宫实验中自发交替率明显降低(78.40±4.16 vs. 67.53±3.08,p<0.05);海马区GFAP阳性细胞数明显增加(133.4±9.6 vs. 280.3±32.7,p<0.01);GFAP蛋白及mRNA表达均显著上调(1.000±0.036vs. 1.300±0.102,p<0.05;1.000±0.031 vs. 1.203±0.078,p<0.05);IL-1α、IL-1β和IL-6 mRNA的表达均明显上调(1.000±0.133 vs. 1.677±0.164,p<0.01;1.000±0.132 vs. 1.488±0.105,p<0.05;1.000±0.218 vs.2.181±0.188,p<0.01)。研究提示,电离辐射可能是通过激活海马Ast诱导炎症因子释放,从而引起认知功能障碍。
作者机构：
[乃爱桃; 艾小红] Department of Radiation Oncology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China;[王五洲; 陈熙; Wan W.; 曹文宇; 王逸轩] Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang, 421001, China;[罗丹; 何洁] Department of Pathology, Medical College, University of South China, Hengyang, 421001, China;[万炜; 何淑雅] Department of Radiation Medicine, School of Public Health, University of South China, Hengyang, 421001, China;[徐杨] Department of Physiology, Medical College, University of South China, Hengyang, 421001, China
通讯机构：
[Xu, Y.; Ai, X.] D;Department of Physiology, China;Department of Radiation Oncology, China
关键词：
电离辐射;前额叶皮质区;NLRP3炎性小体;认知功能障碍
摘要：
探讨电离辐射诱导小鼠认知功能障碍的可能机制.20只雌性昆明小鼠随机分为对照组和辐照组,辐照组小鼠接受137Cs γ射线单次全身辐照至吸收剂量4 Gy.35 d后采用新旧事物识别实验检测小鼠认知功能;免疫组织化学法检测小鼠前额叶皮质区小胶质细胞标记物离子钙接头蛋白-1(IBA-1)的表达;蛋白质印迹法检测前额叶皮质区核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)、半胱氨酸天门冬氨酸蛋白酶-1(Caspase-1)、白细胞介素-1β(IL-1 β)和白细胞介素-18(IL-18)蛋白的表达变化;荧光定量PCR(RT-PCR)检测前额叶皮质IBA-1、NLRP3、ASC和Caspase-1 mRNA的表达情况.结果显示:与对照组相比,辐照组小鼠在新旧事物识别实验中对新事物的分辨率明显降低(38.39 ± 3.69 vs. 28.82 ± 2.08, p<0.05);前额叶皮质区IBA-1阳性细胞数(138.2 ± 3.7 vs. 159.6 ± 6.9, p<0.05)和mRNA表达(1.000 ± 0.031 vs. 1.173 ± 0.055, p<0.05)均显著上调;前额叶皮质区NLRP3、ASC、Caspase-1蛋白(1.000 ± 0.066 vs. 1.341 ± 0.119, p<0.05;1.000 ± 0.073 vs. 1.298 ± 0.083, p<0.05;1.000 ± 0.039 vs. 1.603 ± 0.159, p<0.01)及NLRP3、 ASC mRNA表达(1.000 ± 0.046 vs. 1.372 ± 0.071, p<0.01; 1.000 ± 0.068 vs. 1.225 ± 0.069, p<0.05)均明显上调;前额叶皮质区炎性细胞因子IL-1β和IL-18的表达(1.000 ± 0.033 vs. 1.167 ± 0.059, p<0.05;1.000 ± 0.196 vs. 1.614 ± 0.163, p<0.05)均明显上调.结果提示,电离辐射可能是通过激活前额叶皮质区小胶质细胞,活化NLRP3炎性小体,诱导炎症因子释放引起认知功能障碍的.
