期刊论文

Ionizing radiation induces cognitive dysfunction by activating hippocampal astrocytes in mice

Wan, W.

中文

辐射研究与辐射工艺学报

1000-3436

2019

37

1

20-26

10.11889/j.1000-3436.2019.rrj.37.010301

2018年南华大学研究生科学基金项目（ 2018 KYY256、2018KYY258、2018KYZ065、2018KYZ069 ）资助 第一作者：王贞，女，1990 年7月出生，2015 年毕业于南华大学，现为 南华大学医学院应用解剖与生殖医学研究所硕士研究生， E-mail: 736887612@qq.com 通信作者：万炜，副教授，硕士生导师，E-mail: david-wan@163.com；曹文宇，博士，讲师，E-mail: marksman0@163.com 收稿日期：初稿 2018-09-03；修回 2018-1 1-29 Supported by the Hunan Provincial Natural Science Foundation of China (2018JJ3460, 2018JJ3429, 2018JJ3464), the Hunan Provincial Natural Science Fund Provincial and Municipal Joint Fund Project (2018JJ4034), the Hunan Provincial Education Department Foundation of China (17B230), Planning Project of Hunan Provincial Health and Family Planning Commission Research Projects (C2016042), the Start-up Funds for Ph.D.s in University of South China (2015XQD27, 2015XQD28), Applied Anatomy and Reproductive Medicine Key Laboratory Construction Project in Hengyang (2017KJ182), and Graduate Student Research Innovation Project of University of South China in 2018 (2018KYY256, 2018KYY258, 2018KYZ065, 2018KYZ069) First author: W ANG Zhen (female) was born in July 1990, and graduated from University of South China in 2015. Now she is a mastercandidateintheClinicalAnatomyandReproductiveMedicineApplicationInstituteofUniversityofSouthChina,E-mail: 736887612@qq.com Corresponding author: W AN W ei , associate professor , master’ s supervisor , E-mail:david-wan@163.com;Ph.D.CAO W enyu , Lecturer , E-mail: marksman0@163.com

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探讨电离辐射引起小鼠认知功能障碍的可能机制。24只雌性昆明小鼠随机分为对照组和辐照组,辐照组小鼠接受137Csγ射线单次全身辐照至吸收剂量4 Gy。35 d后采用Y迷宫实验检测小鼠认知功能;免疫组织化学法检测小鼠海马区星形胶质细胞(Astrocytes,Ast)标记物胶质纤维酸性蛋白(Glial fibrillary acidic protein,GFAP)阳性细胞数的表达变化;Western blot方法检测海马GFAP蛋白的表达变化;荧光定量PCR检测炎性因子白细胞介素IL-1α、IL-1β、IL-6 mRNA和海马GFAP的表达情况。结果表明:与对照组相比,辐照组小鼠在Y迷宫实验中自发交替率明显降低(78.40±4.16 vs. 67.53±3.08,p

In order to explore the possible mechanisms underlying cognitive dysfunction induced by ionizing radiation in mice, twenty-four female Kunming mice were randomly divided into a control group and irradiation group (IR). The IR group mice were irradiated with 137Cs γ-rays at an absorbed dose of 4 Gy. After 35 d, the Y-maze test was used to evaluate the cognitive function of the mice. The expression of the astrocyte marker, glial fibrillary acidic protein (GFAP), was detected using immunohistochemical staining. The expression of GFAP protein in t...