期刊论文

Ionizing radiation induces cognitive dysfunction by activating prefrontal cortex NLRP3 inflammasome in mice

Ai, X.;Xu, Y.

中文

辐射研究与辐射工艺学报

1000-3436

2019

37

4

40207-40214

10.11889/j.1000-3436.2019.rrj.37.040301

基金资助：湖南省自然科学基金项目（ 2018 JJ3460、2018JJ4034 ） 、湖南省教育厅项目（ 16 A187、17B230 ） 、南华大学博士 科研启动基金（ 2015 XQD27、2015XQD28 ） 、应用解剖与生殖医学衡阳市重点实验室建设项目（ 2017 KJ182 ） 、南华大学大 学生研究性学习和创新性实验计划项目（ 2018 XJXZ390 ） 、湖南省研究生科研创新项目（ CX 2018B638 ）资助 第一作者：乃爱桃， 男， 1992 年 7 月出生， 2015 年毕业于南华大学， 现为南华大学附属第一医院肿瘤放疗科专业硕士 研究生， E-mail : 499108712@qq.com 通信作者：艾小红，副教授，硕士生导师，E-mail: 1 16304926 @qq.com；徐杨，博士，讲师，E-mail: xuyang21 17 @163.com 收稿日期：初稿 2019-01-03；修回 2019-04-1 1 Supported by the Natural Science Foundation of Hunan Province of China (2018JJ3460, 2018JJ4034), the Education Department Foundation of Hunan Province of China (16A187, 17B230), the Start-up Funds for PhDs in the University of South China (2015XQD27, 2015XQD28), Applied Anatomy and Reproductive Medicine Key Laboratory Construction Project in Hengyang (2017KJ182), the Research-based Learning and Innovation Experimental Project for Undergraduate Student of the University of South China (2018XJXZ390), Hunan Provincial Innovation Foundation for Postgraduate (CX2018B638) First author: NAI Aitao(male) was born in July 1992, and graduated from University of South China in 2015. Now he is a master candidate at the Department of Radiation Oncology , the First Affiliated Hospital of University of South China. E-mail: 499108712@qq.com Corresponding author: AI Xiaohong, associate professor , master’ s supervisor , E-mail: 1 16304926 @qq.com; Ph.D. XU Y ang , lecturer , E-mail: xuyang21 17 @163.com

本校为第一机构

探讨电离辐射诱导小鼠认知功能障碍的可能机制.20只雌性昆明小鼠随机分为对照组和辐照组,辐照组小鼠接受137Cs γ射线单次全身辐照至吸收剂量4 Gy.35 d后采用新旧事物识别实验检测小鼠认知功能;免疫组织化学法检测小鼠前额叶皮质区小胶质细胞标记物离子钙接头蛋白-1(IBA-1)的表达;蛋白质印迹法检测前额叶皮质区核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)、半胱氨酸天门冬氨酸蛋白酶-1(Caspase-1)、白细胞介素-1β(IL-1 β)和白细胞介素-18(IL-18)蛋白的表达变化;荧光定量PCR(RT-PCR)检测前额叶皮质IBA-1、NLRP3、ASC和Caspase-1 mRNA的表达情况.结果显示:与对照组相比...

To explore the potential mechanism of cognitive impairment induced by ionizing radiation,twenty Kunming female mice were randomly divided into control group and irradiation group. The mice in the irradiation group received a single whole body irradiation of 137Cs γ -rays at an absorbed dose of 4 Gy. The novel object recognition test was used to evaluate the cognitive function of mice 35 d after irradiation. The expression of the microglial marker ionized calcium binding adapter molecule-1 (IBA-1) in the prefrontal cortex was determined by immu...