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NLRP3炎性小体抑制剂MCC950对辐射所致认知障碍的保护作用

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成果类型:
期刊论文
论文标题(英文):
MCC950, a NLRP3 inflammasome inhibitor, prevents radiation-induced cognitive impairment in mice
作者:
刘政海;罗诗诗;王贞;徐杨;何洁;...
通讯作者:
Cao, W.
作者机构:
[刘政海; 李彩; 王五洲; 曹文宇; 罗诗诗] Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang, 421001, China
[徐杨] Department of Physiology, Medical College, University of South China, Hengyang, 421001, China
[何洁] Department of Pathology, Medical College, University of South China, Hengyang, 421001, China
[Wang W.; 何淑雅] School of Public Health, University of South China, Hengyang, 421001, China
[艾小红] Department of Radiation Oncology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China
通讯机构:
[Cao, W.] C
Clinical Anatomy & Reproductive Medicine Application Institute, China
语种:
中文
关键词:
辐射;认知损伤;NLRP3炎性小体;小鼠
关键词(英文):
Cognitive impairment;Irradiation;Mice;NLRP3 inflammasome
期刊:
中华放射医学与防护杂志
ISSN:
0254-5098
年:
2020
卷:
40
期:
10
页码:
733-739
基金类别:
Hunan Provincial Natural Science Foundation of China (2019JJ40242, 2019JJ40257, 2019JJ50504, 2020JJ4543).
机构署名:
本校为第一且通讯机构
院系归属:
医学院
公共卫生学院
摘要:
目的:探讨MCC950对辐射所致小鼠认知障碍的影响及可能机制。方法:小鼠按随机数表法分为健康对照(NS)组、全身照射(IR)组和照射后MCC950干预(IR+MCC950)组,每组15只。IR组和IR+MCC950组小鼠给予 137Cs单次4.0 Gy照射,吸收剂量率为1.118 Gy/min,NS组不接受照射。IR+MCC950组小鼠从照射后3周开始腹腔注射MCC950,每日1次,每次10 mg/kg。新旧事物识别等方法检测小鼠认知功能;免疫组织化学法检测小鼠海马CA3区NeuN蛋白的表达;PCR及Western blot检测NLRP3炎性小体相关蛋白的表达。 结果:与NS组相比,IR组小鼠短时及长期新旧事物识别指数显著降低( t=4.321、5.473, P
摘要(英文):
Objective:To investigate the effect of MCC950 (a NLRP3 inflammasome inhibitor) on cognitive impairment in mice with radiation-induced inflammatory brain injury.Methods:Mice were divided into normal (NS) group, whole body irradiation (IR) group and MCC950 intervention post irradiation (IR+ MCC950) group according to the random number table method, with 15 mice in each group. The mice in IR group and IR+ MCC950 group were irradiated with a single dose of 4.0 Gy. The radiation source was 137Cs and the dose rate was 1.118 Gy/min. The mice in NS g...

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