Neuroprotective effects of hydrogen sulfide and the underlying signaling pathways
作者：
Chen, Wen-Lin;Niu, Ying-Ying;Jiang, Wei-Zheng;Tang, Hui-Lan;Zhang, Chong;...
期刊：
Reviews in the Neurosciences ,2015年26(2):129-142 ISSN：0334-1763
通讯作者：
Tang, Xiao-Qing
作者机构：
[Chen, Wen-Lin; Tang, Hui-Lan; Jiang, Wei-Zheng; Niu, Ying-Ying; Xia, Qi-Ming; Zhang, Chong] Univ South China, Coll Med, Inst Neurosci, Hengyang 421001, Hunan, Peoples R China.;[Tang, Xiao-Qing] Univ South China, Coll Med, Inst Neurosci, Hengyang, Hunan, Peoples R China.;[Chen, Wen-Lin; Tang, Hui-Lan; Jiang, Wei-Zheng; Xia, Qi-Ming; Zhang, Chong] Univ South China, Coll Med, Grade Clin Med 2009, Hengyang 421001, Hunan, Peoples R China.;[Niu, Ying-Ying] Univ South China, Coll Med, Grade Clin Med 2011, Hengyang 421001, Hunan, Peoples R China.;[Tang, Xiao-Qing] Univ South China, Coll Med, Inst Neurosci, 28 W Changsheng Rd, Hengyang, Hunan, Peoples R China.
通讯机构：
[Tang, Xiao-Qing] U;Univ South China, Coll Med, Inst Neurosci, 28 W Changsheng Rd, Hengyang, Hunan, Peoples R China.
关键词：
central nervous system diseases;hydrogen sulfide;neuroprotective;signaling pathways
摘要：
Hydrogen sulfide (H2S) is an endogenously produced gas that represents a novel third gaseous signaling molecule, neurotransmitter and cytoprotectant. Cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CSE), 3-mercaptopyruvate sulfur transferase with cysteine aminotransferase (3-MST/CAT) and 3-mercaptopyruvate sulfur transferase with D-amino acid oxidase (3-MST/DAO) pathways are involved in the generation of endogenous H2S despite the ubiquitous or restricted distribution of those enzymes. CBS, 3-MST/CAT and 3-MST/DAO can be found in the brain, while CSE is widely located in other organs. There also exist up-taking or recycling and scavenging mechanisms in H2S metabolism to maintain its persistence for physiological function. In recent years, investigating the role that H2S plays in the central nervous system and cardiovascular system has always been a hotspot. To date, effects of H2S are at least partially verified in multiple animal models or neuron cell lines of Alzheimer's disease, Parkinson's disease, cerebral ischemia, major depression disorders and febrile seizure, although subsequent studies are still badly needed. This article presents an overview of current knowledge of H2S focusing on its neuroprotective effects and corresponding signaling pathways, together with connections to potential therapeutic strategies in the clinic.
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英文
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Mipu1 inhibits lipid accumulation through down-regulation of CD36 in RAW264.7 cells
作者：
Qu, Shun-Lin;Fan, Wen-Jing;Zhang, Chi;Guo, Fang;Pan, Wen-Jun;...
期刊：
Cellular Physiology and Biochemistry ,2015年37(3):879-889 ISSN：1015-8987
通讯作者：
Jiang, Zhi-Sheng
作者机构：
[Jiang, Zhi-Sheng; Qu, Shun-Lin; Guo, Fang; Li, Wei; Zhang, Chi; Han, Dan; Pan, Wen-Jun; Zhu, Yu-Ning; Fan, Wen-Jing] Univ South China, Postdoctoral Mobile Stat Basic Med, Inst Cardiovasc Dis, Hengyang 421001, Hunan, Peoples R China.;[Jiang, Zhi-Sheng; Qu, Shun-Lin; Guo, Fang; Li, Wei; Zhang, Chi; Han, Dan; Pan, Wen-Jun; Zhu, Yu-Ning; Fan, Wen-Jing] Univ South China, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Fan, Wen-Jing] Univ South China, Affiliated Hosp 2, Hengyang 421001, Hunan, Peoples R China.;[Jiang, Zhi-Sheng] Univ South China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Jiang, Zhi-Sheng] U;Univ South China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
CD36;Lipid accumulation;Macrophages;Mipu1;OxLDL
摘要：
Background/Aims: Our recent data indicated that Mipu1 overexpression reduces lipid intake and CD36 expression of macrophages in the presence of oxLDL. However, the mechanism of Mipu1 inhibiting lipid accumulation in macrophages is not elucidated. Methods: Realtime quantitative polymerase chain reaction (PCR) and western blot analysis were used to detect expression of Mipu1 and CD36. The promoter activity of CD36 was studied using luciferase assays. Chromatin immunoprecipitation (ChIP) was used to show the recruitment of Mipu1 onto the CD36 promoter. High-performance liquid chromatography and Dil-labeled lipoprotein were used to detect cholesterol accumulation. Results: Here, we show that CD36 overexpression rescues oxLDL-induced cholesterol accumulation in RAW264.7-Mipu1 cells. Analysis of the mouse CD36 promoter revealed two potential Mipu1-response elements (MRE), one of which (from-237bp to-244bp, ACTTAC) was shown, using mutagenesis and deletion analysis, to be functional. Mipu1 was demonstrated to bind to CD36 promoter, and oxLDL treatment resulted in increases in their interaction as assessed by ChIP. Conclusions: It was demonstrated that Mipu1 inhibited the lipid accumulation of macrophages and it down-regulated CD36 expression in the presence of oxLDL. Copyright © 2015 S. Karger AG, Basel.
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英文
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H2S, a novel therapeutic target in renal-associated diseases?
作者：
Pan, Wen-Jun;Fan, Wen-Jing;Zhang, Chi;Han, Dan;Qu, Shun-Lin* ;...
期刊：
Clinica Chimica Acta ,2015年438:112-118 ISSN：0009-8981
通讯作者：
Qu, Shun-Lin
作者机构：
[Jiang, Zhi-Sheng; Qu, Shun-Lin; Zhang, Chi; Han, Dan; Pan, Wen-Jun; Fan, Wen-Jing] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Postdoctoral Mobile Stn Basic Med, Hengyang City 421001, Hunan, Peoples R China.;[Fan, Wen-Jing] Univ South China, Affiliated Hosp 2, Hengyang City 421001, Hunan, Peoples R China.;[Qu, Shun-Lin] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Qu, Shun-Lin] U;Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Antioxidant;Cellular matrix remodeling;Hydrogen sulfide;Inflammation;Renal-associated diseases
摘要：
For more than a century, hydrogen sulfide (H2S) has been regarded as a toxic gas. Recently, the understanding of the biological effects of H2S has been changed. This review surveys the growing recognition of H2S as an endogenous signaling molecule in mammals, with emphasis on its physiological and pathological pathways in the urinary system. This article reviews recent progress of basic and pharmacological researches related to endogenous H2S in urinary system, including the regulatory effects of H2S in the process of antioxidant, inflammation, cellular matrix remodeling and ion channels, and the role of endogenous H2S pathway in the pathogenesis of renal and urogenital disorders. © 2014.
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英文
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Myocardial ischemic preconditioning upregulated protein 1(Mipu1):Zinc finger protein 667 – A multifunctional KRAB/C2H2 zinc finger protein
作者：
Han, D.;Zhang, C.;Fan, W. J.;Pan, W. J.;Feng, D. M.;...
期刊：
Brazilian Journal of Medical and Biological Research ,2015年48(1):1-5 ISSN：0100-879X
通讯作者：
Qu, S. L.
作者机构：
[Zhang, C.; Pan, W. J.; Fan, W. J.; Han, D.; Jiang, Z. S.; Qu, S. L.; Feng, D. M.] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Postdoctoral Mobile Stn Basic Med, Hengyang City, Hunan, Peoples R China.;[Fan, W. J.] Univ South China, Affiliated Hosp 2, Hengyang City, Hunan, Peoples R China.
通讯机构：
[Qu, S. L.] U;Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Postdoctoral Mobile Stn Basic Med, Hengyang City, Hunan, Peoples R China.
关键词：
Mipu1;Zinc finger structure;Nuclear factor;Transcription and repression
摘要：
Myocardial ischemic preconditioning upregulated protein 1 (Mipu1) is a newly discovered upregulated gene produced in rats during the myocardial ischemic preconditioning process. Mipu1 cDNA contains a 1824-base pair open reading frame and encodes a 608 amino acid protein with an N-terminal Kru¨ ppel-associated box (KRAB) domain and classical zinc finger C2H2 motifs in the C-terminus. Mipu1 protein is located in the cell nucleus. Recent studies found that Mipu1 has a protective effect on the ischemia-reperfusion injury of heart, brain, and other organs. As a nuclear factor, Mipu1 may perform its protective function through directly transcribing and repressing the expression of proapoptotic genes to repress cell apoptosis. In addition, Mipu1 also plays an important role in regulating the gene expression of downstream inflammatory mediators by inhibiting the activation of activator protein-1 and serum response element. © 2015, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.
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英文
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RETRACTED: Betulinic acid downregulates expression of oxidative stress-induced lipoprotein lipase via the PKC/ERK/c-Fos pathway in RAW264.7 macrophages
作者：
Peng, Juan;Lv, Yun-Cheng;He, Ping-Ping;Tang, Yan-Yan;Xie, Wei;...
期刊：
Biochimie ,2015年119:192-203 ISSN：0300-9084
通讯作者：
Yin, Wei-Dong
作者机构：
[Lan, Gan; He, Ping-Ping; Shi, Jin-Feng; Peng, Juan; Zhang, Min; Li, Yuan; Lv, Yun-Cheng; Tang, Chao-Ke; Yin, Wei-Dong; Xie, Wei; Tang, Yan-Yan; Zhang, Chi] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.;[Peng, Juan] Yongzhou Vocat & Tech Coll, Yongzhou 425000, Hunan, Peoples R China.;[He, Ping-Ping] Univ South China, Sch Nursing, Hengyang 421001, Hunan, Peoples R China.;[Liu, Xiang-Yu] Univ South, Sch Life Sci & Technol, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.;[Zheng, Xi-Long] Univ Calgary, Dept Biochem & Mol Biol, Libin Cardiovasc Inst Alberta, Cumming Sch Med, Calgary, AB T2N 4N1, Canada.
通讯机构：
[Yin, Wei-Dong] U;Univ South China, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词：
Atherosclerosis;Betulinic acid;LPL;Oxidative stress
摘要：
Background Atherosclerosis is a major cause of coronary artery disease, which is characterized by cellular lipid accumulation. Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism. Studies have shown that macrophage-derived LPL exhibits proatherogenic properties, and plays a major role in lipid accumulation in macrophages. Evidence suggests that oxidative stress can effectively enhance macrophage LPL production. Betulinic acid (BA) is a pentacyclic lupane triterpene with a potent antioxidant activity. In this study, we investigated whether BA affects the expression of macrophage LPL and how it regulates cellular lipid accumulation. Methods and results We revealed that BA downregulated H2O2-simulated macrophage LPL protein, mRNA levels and its activity in both concentration- and time-dependent manners. Furthermore, BA decreased LPL-involved total cholesterol and triglyceride levels in macrophages. In addition, cellular lipid staining by Oil Red O showed that BA decreased cellular lipid droplet deposition. Next, we confirmed that pretreatment with BA decreased H2O2-induced production of intracellular reactive oxygen species in a concentration-dependent manner. Further studies demonstrated that BA inhibited H2O2-induced membrane translocation of PKC, phosphorylation of ERK1/2 and c-Fos. Finally, the induction of LPL production and activity by H2O2 was abolished by BA, inhibition of PKC or ERK or depletion c-Fos, respectively. Conclusions BA, through its role of antioxidant activity, attenuated macrophage-derived LPL expression and activity induced by oxidative stress, and effectively reduced cellular lipid accumulation, likely through inhibition of the pathways involving PKC, ERK and c-Fos. These effects of BA may contribute to its mitigation of atherosclerosis and help develop BA as a therapeutic compound in treatment of atherosclerosis. © 2015 Published by Elsevier B.V.
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英文
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MiR-493 suppresses the proliferation and invasion of gastric cancer cells by targeting RhoC
作者：
Zhou, Wenhua;Zhang, Chi;Jiang, Hao;Zhang, Zhiwei;Xie, Liming;...
期刊：
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES ,2015年18(10):1027-1033 ISSN：2008-3866
通讯作者：
He, Xiusheng
作者机构：
[Zhou, Wenhua; Zhang, Zhiwei; He, Xiusheng] Univ South China, Canc Res Inst, Key Lab Canc Cellular & Mol Pathol Hunan Prov Uni, Hengyang 421001, Peoples R China.;[Zhang, Chi] Univ South China, Sch Med, Funct Lab Ctr, Hengyang 421001, Hunan, Peoples R China.;[Xie, Liming; Jiang, Hao] Univ South China, Affiliated Hosp 1, Ctr Gastr Canc Res Human Prov, Hengyang 421001, Hunan, Peoples R China.;[He, Xiusheng] Univ South China, Canc Res Inst, 28 West Changsheng Rd, Hengyang 421001, Peoples R China.
通讯机构：
[He, Xiusheng] U;Univ South China, Canc Res Inst, 28 West Changsheng Rd, Hengyang 421001, Peoples R China.
关键词：
Gastric cancer;Invasion;Prognostic significance;Proliferation;miR-493
摘要：
Objective(s): MiRNAs have been proposed to be key regulators of tumorigenesis, progression and metastasis. However, their effect and prognostic value in gastric cancer is still poorly known. Materials and Methods: Gastric cancer cell lines were cultured. Tissue samples obtained from 36 gastric cancer patients were used for quantitative real‐time PCR (qRT‐PCR) analysis. The tissue microarrays (TMAs) consisted of 126 cases of gastric carcinoma that were used for In situ hybridisation (ISH). Lentivirus plasmids were co‐transfected into 293FT cells. Cell migration was examined using wound‐healing assays. Statistical analyses were performed using SPSS16.0 software. Results: In this study, we found that the expression levels of miR‐493 were strongly down‐regulated in gastric cancer and were associated with clinical stage and the presence of lymph node metastases. Moreover, miR‐493 might independently predict OS and RFS in gastric cancer. We further found that up‐regulation of miR‐493 inhibited the proliferation and metastasis of gastric cancer cells, in vitro and in vivo. In addition, miR‐493 directly targeted RhoC, which resulted in a marked reduction of the expression of mRNA and protein. This effect, in turn, led to a decreased ability of growth, invasion and metastasis in gastric cancer cells. Conclusion: Taken together, our findings demonstrate that miR‐493 is important for gastric cancer initiation and progression and holds promise as a prognostic biomarker to predict survival and relapse in gastric cancer. It is also a potential therapeutic tool to improve clinical outcomes in this disease. © 2015, Mashhad University of Medical Sciences. All rights reserved.
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英文
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Integrated omics study delineates the dynamics of lipid droplets in Rhodococcus opacus PD630
作者：
Chen, Yong;Ding, Yunfeng;Yang, Li;Yu, Jinhai;Liu, Guiming;...
期刊：
Nucleic Acids Research ,2014年42(2):1052-1064 ISSN：0305-1048
通讯作者：
Yu, Jun
作者机构：
[Yang, Fuquan; Huo, Chaoxing; Zhang, Peng; Zhang, Huina; Yang, Li; Ding, Yunfeng; Xie, Zhensheng; Zhang, Shuyan; Xu, Shimeng; Liu, Pingsheng; Chen, Yong; Zhang, Congyan; Wang, Yang; Na, Huimin; Yu, Jinhai] Chinese Acad Sci, Inst Biophys, Natl Lab Macromol, Beijing 100101, Peoples R China.;[Chen, Yong; Zhang, Michael Q.; Li, Yanda] Tsinghua Univ, TNLIST, Ctr Synthet & Syst Biol, Dept Automat,MOE Key Lab Bioinformat, Beijing 100084, Peoples R China.;[Chen, Yong; Zhang, Michael Q.; Li, Yanda] Tsinghua Univ, TNLIST, Ctr Synthet & Syst Biol, Dept Automat,Bioinformat Div, Beijing 100084, Peoples R China.;[Huo, Chaoxing; Zhang, Peng; Yang, Li; Song, Lai; Xu, Shimeng; Zhang, Congyan; Wang, Yang; Zhang, Yue; Na, Huimin; Yu, Jinhai] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Liu, Guiming; Huo, Linhe; Song, Lai; Wang, Xumin; Wang, Guoliang; Yu, Jun; Zhang, Hangxiao; Yu, Dan; Fan, Zhonghua; Zhang, Yue] Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China.
通讯机构：
[Yu, Jun] C;Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China.
摘要：
Rhodococcus opacus strain PD630 (R. opacus PD630), is an oleaginous bacterium, and also is one of few prokaryotic organisms that contain lipid droplets (LDs). LD is an important organelle for lipid storage but also intercellular communication regarding energy metabolism, and yet is a poorly understood cellular organelle. To understand the dynamics of LD using a simple model organism, we conducted a series of comprehensive omics studies of R. opacus PD630 including complete genome, transcriptome and proteome analysis. The genome of R. opacus PD630 encodes 8947 genes that are significantly enriched in the lipid transport, synthesis and metabolic, indicating a super ability of carbon source biosynthesis and catabolism. The comparative transcriptome analysis from three culture conditions revealed the landscape of gene-altered expressions responsible for lipid accumulation. The LD proteomes further identified the proteins that mediate lipid synthesis, storage and other biological functions. Integrating these three omics uncovered 177 proteins that may be involved in lipid metabolism and LD dynamics. A LD structure-like protein LPD06283 was further verified to affect the LD morphology. Our omics studies provide not only a first integrated omics study of prokaryotic LD organelle, but also a systematic platform for facilitating further prokaryotic LD research and biofuel development.
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英文
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新型褪黑素受体激动剂Neu-P11激活AMPK减轻心肌细胞缺氧/复氧损伤
作者：
黄靓;王汉群;张弛;高勇强;向琼;...
期刊：
中国药理学通报 ,2014年30(1):104-108 ISSN：1001-1978
作者机构：
[王平平; Chi Z.; 尹卫东] Institute of Cardiovascular Diseases, University of South China, Hengyang Hunan 421001, China;[向琼; 高勇强; 王汉群] Dept. of Operative Surgery, University of South China, Hengyang Hunan 421001, China;[Huang L.] Institute of Cardiovascular Diseases, University of South China, Hengyang Hunan 421001, China, Dept. of Operative Surgery, University of South China, Hengyang Hunan 421001, China
关键词：
腺苷酸活化蛋白激酶;心肌细胞;缺氧;复;氧损伤;腺苷酸活化蛋白激酶;细胞凋亡;褪黑素
摘要：
目的 研究新型褪黑素受体激动剂Neu-P11对心肌细胞缺氧/复氧损伤的作用及分子机制.方法 实验分为对照组、缺氧/复氧模型组(H/R组)、H/R+Neu-P11组、H/R+Compound C组、H/R+Neu-P11+Compound C组.构建H9c2心肌细胞缺氧/复氧模型,检测各组心肌细胞凋亡情况,细胞培养液中乳酸脱氢酶(LDH)、肌酸激酶(CK)、SOD活性及MDA含量及心肌细胞AMPK的活性.结果 与缺氧/复氧组细胞相比,Neu-P11预处理组细胞凋亡率明显减少,CK、LDH、MDA含量明显下降,SOD活性增加,心肌细胞AMPK活性明显增高,而AMPK特异性抑制剂Compound C可以阻断Neu-P11的保护作用.结论 Neu-P11能够减轻心肌细胞缺氧/复氧损伤,这一保护作用与激活AMPK有关.
语种：
中文
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Melatonin rescues 3T3-L1 adipocytes from FFA-induced insulin resistance by inhibiting phosphorylation of IRS-1 on Ser307
作者：
She, Meihua;Hou, Hongjie;Wang, Zongbao;Zhang, Chi;Laudon, Moshe;...
期刊：
Biochimie ,2014年103(1):126-130 ISSN：0300-9084
通讯作者：
Yin, Weidong
作者机构：
[Yin, Weidong; Zhang, Chi; She, Meihua] Univ South China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.;[Hou, Hongjie; Yin, Weidong; She, Meihua] Univ South China, Dept Biochem & Mol Biol, Hengyang 421001, Peoples R China.;[Wang, Zongbao] Univ South China, Dept Lab Anim Sci, Hengyang 421001, Peoples R China.;[Laudon, Moshe] Neurim Pharmaceut Ltd, Drug Discovery, Tel Aviv, Israel.
通讯机构：
[Yin, Weidong] U;Univ South China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.
关键词：
3T3-L1 adipocytes;Glucose uptake;Insulin sensitivity;Melatonin
摘要：
Melatonin is biosynthesized in the pineal gland and secreted into the bloodstream. Evidences indicate a role of melatonin in the regulation of glucose metabolism. The objective of this study was to investigate the effect of melatonin on insulin sensitivity in insulin resistant adipocytes. Following a preincubation with melatonin or vehicle for 30 min, insulin resistant cells of 3T3-L1 adipocytes were induced by palmitic acids (300 mu M, 6 h). Our results showed that palmitic acids inhibited both the basal and insulin-stimulated uptake of [H-3]-2-Deoxyglucose, down-regulated the levels of IRS-1 and GLUT-4. However, compared to the vehicle group, melatonin pre-treatment increased significantly the uptake of [H-3]-2-Deoxyglucose as well as the level of GLUT-4, and decreased phosphorylated IRS-1 (Ser307) although total IRS-1 did not change significantly. These data suggest that palmitic acids impair insulin signal via down-regulating the expressions of IRS-1 and GLUT-4; whereas melatonin can ameliorate insulin sensitivity by inhibiting Ser307 phosphorylation in IRS-1 and increasing GLUT-4 expressions in insulin resistant 3T3-L1 adipocytes. We conclude that melatonin regulates the insulin sensitivity and glucose homeostasis via inhibiting Ser-phosphorylation and improving function of IRS-1. (C) 2014 Elsevier Masson SAS. All rights reserved.
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英文
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SIRT1 in cardiovascular aging
作者：
Luo, Xin-Yuan;Qu, Shun-Lin;Tang, Zhi-Han;Zhang, Yuan;Liu, Mi-Hua;...
期刊：
Clinica Chimica Acta ,2014年437:106-114 ISSN：0009-8981
通讯作者：
Jiang, Zhi-Sheng
作者机构：
[Tang, Hui; Jiang, Zhi-Sheng; Ren, Zhong; Tang, Zhi-Han; Qu, Shun-Lin; Liu, Mi-Hua; Yu, Kang-Lun; Zhang, Yuan; Zhang, Chi; Peng, Juan; Luo, Xin-Yuan] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Jiang, Zhi-Sheng] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Jiang, Zhi-Sheng] U;Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Anti-aging strategies;Autophagy;Cardiovascular aging;Inflammation;Oxidative stress;SIRT1
摘要：
Cardiovascular disease (CVD) is the leading cause of death worldwide, with aging as the key independent risk factor. Effective interventions are necessary to delay aging. Sirtuin1 (SIRT1), a NAD+-dependent histone deacetylase, is closely related to lifespan extension. SIRT1 exerts beneficial effects on aging and age-related diseases, such as atherosclerosis. In this review, we summarize the current knowledge on the functions of SIRT1 in cardiovascular aging, focusing on the underlying molecular mechanisms, including inhibition of oxidative stress and inflammation, and induction of autophagy. We also demonstrate that moderate up-regulation or activation of SIRT1 in cardiovascular aging and age-related CVD may confer important application values. © 2014 Elsevier B.V.
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英文
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Mipu1 overexpression protects macrophages from oxLDL-induced foam cell formation and cell apoptosis
作者：
Qu, Shun-Lin;Fan, Wen-Jing;Zhang, Chi;Guo, Fang;Han, Dan;...
期刊：
DNA AND CELL BIOLOGY ,2014年33(12):839-846 ISSN：1044-5498
通讯作者：
Jiang, Zhi-Sheng
作者机构：
[Jiang, Zhi-Sheng; Qu, Shun-Lin; Guo, Fang; Li, Wei; Zhang, Chi; Han, Dan; Feng, Da-Ming; Pan, Wen-Jun; Fan, Wen-Jing] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Postdoctoral Mobile Stat Basic Med, Hengyang City 421001, Hunan, Peoples R China.;[Fan, Wen-Jing] Univ South China, Affiliated Hosp 2, Hengyang City 421001, Hunan, Peoples R China.;[Jiang, Zhi-Sheng] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, 28 West Changsheng Rd, Hengyang City 421001, Hunan, Peoples R China.
通讯机构：
[Jiang, Zhi-Sheng] U;Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, 28 West Changsheng Rd, Hengyang City 421001, Hunan, Peoples R China.
摘要：
Mipu1 (myocardial ischemic preconditioning upregulated protein 1) is a novel N-terminal Kruppel-associated box (KRAB)/C2H2 zinc finger superfamily protein, that displays a powerful effect in protecting H9c2 cells from oxidative stress-induced cell apoptosis. The present study aims to investigate the effect of Mipu1 overexpression on oxidized low-density lipoprotein (oxLDL)-induced foam cell formation, cell apoptosis, and its possible mechanisms. New Zealand healthy rabbits were used to establish atherosclerosis model, and serum levels of triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were detected by an automatic biochemical analyzer. Sudan IV staining was used to detect atherosclerotic lesions. The RAW264.7 macrophage cell line was selected as the experimental material. Oil red O staining, high-performance liquid chromatography, and Dil-labeled lipoprotein were used to detect cholesterol accumulation qualitatively and quantitatively, respectively. Flow cytometry was used to determine cell apoptosis. Real-time quantitative polymerase chain reaction (PCR) was used to detect the mRNA expression of the main proteins that are associated with the transport of cholesterol, such as ABCA1, ABCG1, SR-BI, and CD36. Western blot analysis was used to detect the protein expression of Mipu1. There were atherosclerotic lesions in the high-fat diet group with Sudan IV staining. High-fat diet decreased Mipu1 expression and increased CD36 expression significantly at the 10th week compared with standard-diet rabbits. Mipu1 overexpression decreased oxLDL-induced cholesterol accumulation, oxLDL uptake, cell apoptosis, and cleaved caspase-3. Mipu1 overexpression inhibited the oxLDL-induced CD36 mRNA and protein expression, but it did not significantly inhibit the mRNA expression of ABCA1, ABCG1, and SR-BI. Mipu1 overexpression inhibits oxLDL-induced foam cell formation and cell apoptosis. Mipu1 overexpression reduces the lipid intake of macrophages and might be associated with the downregulation of CD36 expression in the presence of oxLDL. © Mary Ann Liebert, Inc. 2014.
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英文
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Piromelatine, a novel melatonin receptor agonist, stabilizes metabolic profiles and ameliorates insulin resistance in chronic sleep restricted rats
作者：
She, Meihua;Hu, Xiaobo;Su, Zehong;Zhang, Chi;Yang, Shenghua;...
期刊：
European Journal of Pharmacology ,2014年727(1):60-65 ISSN：0014-2999
通讯作者：
Yin, Weidong
作者机构：
[Hu, Xiaobo; Su, Zehong; Yin, Weidong; Ding, Lin; She, Meihua] Univ South China, Dept Biochem & Mol Biol, Sch Life Sci & Technol, Hengyang 421001, Peoples R China.;[Yin, Weidong; Zhang, Chi; She, Meihua] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Yang, Shenghua] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Hangzhou 310003, Zhejiang, Peoples R China.;[Laudon, Moshe] Neurim Pharmaceut Ltd, Drug Discovery, Tel Aviv, Israel.
通讯机构：
[Yin, Weidong] U;Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.
关键词：
Agonist;Glucose tolerance;Insulin sensitivity;Melatonin;Piromelatine;Sleep restriction
摘要：
Chronic sleep deprivation may speed the onset or increase the severity of age-related conditions such as Type 2 diabetes, high blood pressure and obesity. Piromelatine (Neu-P11) is a novel melatonin agonist, which has been developed for the treatment of insomnia. Animal studies have suggested possible efficacy of piromelatine in sleep maintenance, anxiety and depression. In addition, piromelatine has been shown to inhibit weight gain and improve insulin sensitivity in high-fat/high-sucrose-fed (HFSD) rats. The objective of this study was to investigate the effects of piromelatine on insulin sensitivity in sleep restricted rats. Sleep restriction was established by rotating cages intermittently for 20 h thereby sleeping time of rats was limited to 4 h per day. During 8 days of sleep restriction, rats were injected intraperitoneally with piromelatine (20 mg/kg), melatonin (5 mg/kg) or a vehicle. The results showed that sleep restriction increased plasma glucose, fasting insulin, total cholesterol (TC), triglycerides (TG) and oxidative stress markers while HDL-cholesterol (HDL-C) level and glucose tolerance were decreased. However, under piromelatine or melatonin treatment, the levels of plasma glucose, TG, TC decreased and HDL-C, glucose tolerance and antioxidative potency increased when compared with the vehicle-treated group. These data suggest that chronic sleep restriction in rats induce metabolic dysfunction, oxidative stress and insulin resistance, and these symptoms were improved by treatment with piromelatine or melatonin. We conclude that piromelatine could regulate metabolic profiles and insulin sensitivity, and attenuate insulin resistance induced by sleep restriction. © 2014 Elsevier B.V.
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英文
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设计性机能学实验对医学生自主学习能力培养的探讨
作者：
张弛;屈顺林;韦星;谭健苗;易光辉;...
期刊：
山西医科大学学报 ,2013年15(9):856-858 ISSN：1007-6611
作者机构：
南华大学医学院机能学实验中心,衡阳,421001;南华大学医学院病理生理学教研室
关键词：
设计性机能学实验;教学改革;自主学习能力
摘要：
设计性机能学实验是医学机能学实验课程体系重要的组成部分,是医学生自主学习能力培养的重要途径。通过开展设计性机能学实验,能够帮助医学生树立自主学习意识,激发学习兴趣,选择学习方法与技巧,学会自我评价,从而提高教学质量。
语种：
中文
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全反式维甲酸对人白血病细胞系HL-60细胞增殖的影响
作者：
曾勇智;张弛
期刊：
科技信息 ,2013年(35):34+61 ISSN：1001-9960
作者机构：
南华大学大学医学院机能实验中心
关键词：
全反式维甲酸;HL-60细胞;增殖
摘要：
目的:观察全反式维甲酸(ATRA)对体外培养人白血病细胞系HL-60细胞增殖的影响。方法:体外培养人白血病细胞HL-60细胞,将不同浓度的ATRA作用于HL-60细胞分别12、24h和48h后,MTT法检测ATRA对HL-60的增殖影响,免疫组织化学染色增生细胞核抗原(PCNA)检测HL-60细胞生长活性。结果:MTT法显示10-9mmol/L ATRA作用12h后能抑制HL-60细胞的增殖(P【0.05),并呈剂量和时间依赖性。ATRA能浓度依赖性地抑制HL-60细胞表达PCNA(P【0.05)。结论:ATRA能够显著抑制人白血病细胞HL-60细胞的增殖。
语种：
中文
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医学本科生实验课成绩的评判体系探讨
作者：
赵战芝;谭健苗;张弛;易光辉
期刊：
吉林医学 ,2013年34(26):5530-5532 ISSN：1004-0412
作者机构：
南华大学医学院机能实验中心,湖南,衡阳,421001
关键词：
医学本科生;实验教学;教学管理
摘要：
论文在阐述医学本科生实验课教学的全方位目标、创新素质培养的方式方法的基础上,初步提出了全面考核医学本科生知识、技能和创新素质的实验课成绩评判体系.
语种：
中文
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医学机能实验学形成性考核应用探讨
作者：
张弛;曾勇智;赵战芝;张海涛;刘德平;...
期刊：
山西医科大学学报 ,2013年15(8):771-773 ISSN：1007-6611
作者机构：
南华大学医学院机能学实验中心,衡阳,421001
关键词：
医学机能学实验;教学改革;形成性考核
摘要：
医学机能实验学课程以强化实验技能为重点,以培养创新能力为核心,在医学人才培养体系中发挥了重要的作用。考核体系的改革是医学机能实验学教学改革的重要组成部分。南华大学医学机能学实验课程从形成性考核的考核内容、构成体系及评价指标等几个方面入手对考核体系进行了改革。
语种：
中文
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Antidepressant-like and anxiolytic-like effects of hydrogen sulfide in behavioral models of depression and anxiety
作者：
Chen, Wen-Lin;Xie, Bin;Zhang, Chong;Xu, Kang-Li;Niu, Ying-Ying;...
期刊：
BEHAVIOURAL PHARMACOLOGY ,2013年24(7):590-597 ISSN：0955-8810
通讯作者：
Tang, Xiao-Qing
作者机构：
[Chen, Wen-Lin; Xie, Bin; Niu, Ying-Ying; Tang, Xiao-Qing; Xu, Kang-Li; Zou, Wei; Hu, Bi; Zhang, Chong; Zhang, Ping] Univ South China, Coll Med, Inst Cognit & Nervous Syst Dis, Hengyang 421001, Hunan, Peoples R China.;[Tian, Ying] Univ South China, Coll Med, Dept Biochem, Hengyang 421001, Hunan, Peoples R China.;[Chen, Wen-Lin; Xie, Bin; Niu, Ying-Ying; Xu, Kang-Li; Zhang, Chong] Univ South China, Coll Med, Hengyang 421001, Hunan, Peoples R China.;[Tang, Xiao-Qing] Univ South China, Inst Cognit & Nervous Syst Dis, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Tang, Xiao-Qing] U;Univ South China, Inst Cognit & Nervous Syst Dis, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
anxiety, depression, elevated plus maze, forced swimming test, hydrogen sulfide, mouse, rat, tail suspension test
摘要：
Depression is a common and debilitating mental illness and is often comorbid with anxiety disorders. Altered synaptic plasticity is considered to be an important mechanism underlying antidepressant drug action. It has been reported that hydrogen sulfide (H2S), the third gaseous transmitter, facilitates the induction of hippocampal long-term potentiation and augments synaptic neurotransmission, involved in the regulation of synaptic plasticity. The aim of this study was to clarify the antidepressant-like and anxiolytic-like effects of H2S. H2S (NaHS, 1.68 or 5.6 mg/kg, intraperitoneally, for 7 days) exerts a specific antidepressant-like effect in the forced swimming test of mice and rats and the tail suspension test of mice, and reduces the anxiety-like behaviors of both mice and rats in the elevated plus-maze test. These results reveal a unique antagonistic action of H2S in depressive-like and anxiety-like behaviors and suggest that elevating H2S signaling in the brain may represent a novel approach for the treatment of depressive and anxiety disorders. Behavioural Pharmacology 24:590597 © 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins.
语种：
英文
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二烯丙基二硫诱导人白血病HL-60细胞周期G2/M期阻滞及机制
作者：
曾勇智;张弛;赵战芝
期刊：
中国保健营养(中旬刊) ,2013年(9):46-46 ISSN：1004-7484
作者机构：
南华大学大学医学院机能实验中心 湖南 衡阳 421001
关键词：
二烯丙基二硫;HL-60细胞
摘要：
目的：研究二烯丙基二硫（Dial yl disulfide，DADS）诱导人髓系白血病细胞株HL-60细胞周期阻滞的分子机制。方法：用不同浓度的DADS处理HL-60细胞0，6，12，24，48 h后，用MTT法、细胞集落培养检测细胞增殖活性；用流式细胞术和有丝分裂指数检测细胞增殖周期；蛋白印迹法检测细胞内p53、p21、及Cdc2激酶的表达和磷酸化水平。结果：不同浓度的DADS对HL-60细胞增殖均有抑制作用，抑制率随着浓度明显增加，且有组间差异（P<0.05），20μM DADS与10 nM ATRA抑制细胞增殖活性相近(P>0.05)；20μMDADS处理HL-60细胞12h后出现细胞周期G2/M期阻滞，此时细胞有丝分裂指数明显下降（P<0.05）；同时，p53表达没有改变而p21及磷酸化cdc2蛋白表达明显增加（P<0.05）。结论：DADS能诱导人髓系白血病细胞株HL-60细胞增殖周期G2/M期阻滞，p21的活化及cdc2磷酸化可能是DADS诱导细胞周期阻滞的分子机制之一。
语种：
中文
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虚拟实验系统在医学机能实验学教学中的应用
作者：
张弛;曾勇智;张海涛;易光辉
期刊：
教育教学论坛 ,2013年(33):265-266 ISSN：1674-9324
作者机构：
南华大学 医学院机能学实验中心,湖南 衡阳,421001
关键词：
机能实验学;虚拟实验;真实实验
摘要：
医学机能实验学是基础医学领域的一门实验课程，继承了生理学、药理学和病理生理学实验课程的实验教学内容。在机能实验教学中构建虚拟实验室是为了适应社会发展，本文对其在机能实验中的应用进行了研究与探讨。
语种：
中文
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Blood pressure reducing effects of piromelatine and melatonin in spontaneously hypertensive rats.
作者：
Huang, L.;Zhang, C.;Hou, Y.;Laudon, M.;She, M.;...
期刊：
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES ,2013年17(18):2449-2456 ISSN：1128-3602
通讯作者：
Yin, W.
作者机构：
[Zhang, C.; Yin, W.; Hou, Y.; Huang, L.; He, P.] Univ South China, Key Lab Arteriosclerosis Hunan Prov, Inst Cardiovasc Res, Hengyang, Hunan, Peoples R China.;[Huang, L.; Wang, H.] Univ South China, Dept Operat Surg, Hengyang, Hunan, Peoples R China.;[Yin, W.; She, M.; Ding, L.; Yang, S.] Univ South China, Dept Biochem & Mol Biol, Sch Life Sci & Technol, Hengyang, Hunan, Peoples R China.;[Laudon, M.] Neurim Pharmaceut Ltd, Drug Discovery, Tel Aviv, Israel.;[Wang, Z.] Univ South China, Dept Lab Anim Sci, Hengyang, Hunan, Peoples R China.
通讯机构：
[Yin, W.] U;Univ South China, Key Lab Arteriosclerosis Hunan Prov, Inst Cardiovasc Res, Hengyang, Hunan, Peoples R China.
关键词：
Piromelatine;Melatonin;Hypertension;Spontaneously hypertensive rats;Metabolic diseases
摘要：
Recently, widespread interest has grown regarding melatonin treatment of hypertension including its cardioprotective effects. Studies in rodents indicate that melatonin plays a role in the pathogenesis of hypertension in rats with metabolic syndrome. Piromelatine, a melatonin agonist, serotonin 5-HT-1A and 5-HT-1D agonist and serotonin 5-HT2B antagonist is a multimodal agent with sleep promoting, anti-diabetic, analgesic, anti-neurodegenerative, anxiolytic and antidepressant potential, currently in development for the treatment of insomnia. In this report we assessed the effects of piromelatine and melatonin treatment on blood pressure in spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. Five groups of 12-wk-old rats (10/group) were treated for 5 weeks with a vehicle, piromelatine (5, 15 and 50 mg/kg BW) and melatonin (10 mg/kg BW) and an age-matched WKY control group. Systolic blood pressure (tail-cuff method) was measured weekly at 9:00 a.m. and at 9:00 p.m. The rats body weight, plasma glucose, insulin, triglyceride, adiponectin, total cholesterol, HDL and LDL/VLDL cholesterol were also measured. Our results showed that both piromelatine and melatonin reduced SHR blood pressure significantly both during the morning and the evening. Piromelatine, but not melatonin, also reduced SHR body weight gain and both significantly decreased plasma glucose and insulin levels and increased adiponectin levels. Piromelatine, similar to melatonin, has an antihypertensive effect and also attenuates body weight, improves metabolic profiles and might be useful in the treatment of hypertension and the metabolic syndrome.
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英文
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