作者： Xiao, Xuan;Yang, Chen;Qu, Shun-Lin;Shao, Yi-Duo;Zhou, Chu-Yi;...

期刊： Clinica Chimica Acta,2020年502:293-304 ISSN：0009-8981

通讯作者： Huang, Liang;Zhang, Chi

作者机构： [Shao, Yi-Duo; Huang, Liang; Xiao, Xuan; Zhou, Chu-Yi] Univ South China, Hengyang Med Coll, Res Lab Clin & Translat Med, Hengyang 421001, Hunan, Peoples R China.;[Yang, Chen; Xiao, Xuan; Qu, Shun-Lin; Chao, Ru; Zhang, Chi] Univ South China, Hengyang Med Coll, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Xuan] Univ South China, Hengyang Med Coll, Dept Clin Med, Hengyang 421001, Hunan, Peoples R China.;[Shao, Yi-Duo; Zhou, Chu-Yi] Univ South China, Hengyang Med Coll, Dept Stomatol, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Huang, Liang; Zhang, Chi] U;Univ South China, Hengyang Med Coll, Res Lab Clin & Translat Med, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hengyang Med Coll, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Hunan, Peoples R China.

关键词： Atherosclerosis;RAGE;S100A12;S100A8;S100A9

摘要： Atherosclerosis is an arterial disease associated with dyslipidemia, abnormal arterial calcification and oxidative stress. It has been shown that a continued chronic inflammatory state of the arterial wall contributes to the development of atherosclerosis. The inflammatory stimulation, recruitment of inflammatory cells and production of pro-inflammatory cytokines enhances vascular inflammation. Some members of the S100 proteins family bind with their receptors, such as advanced glycation end products (RAGE), scavenger receptors (CD36) and toll-like receptor 4 (TLR-4), contributing to the cellular response in atherosclerotic progression. This review summarizes the roles of S100 proteins (S100A8, S100A9 and S100A12) in the vascular inflammation, vascular calcification and vascular oxidative stress. S100 proteins are released from monocytes, smooth muscle cells and endothelial cells in response to cellular stress stimuli, and then the binding of S100 proteins to RAGE activate downstream signaling such as transcription factor kappa B (NF-κB) translocation and reactive oxygen species (ROS) production, which act as a positive feedback loop for inducing pro-inflammatory phenotype in a wide variety of cell types including endothelial cells, vascular smooth muscle cells and leukocytes. Thus, it suggests that the inhibition of S100 proteins-mediated RAGE and TLR4 activation appears to be a promising approach to treat atherosclerosis. In addition, recent study showed that serum S100A12 can predict future cardiovascular events, highlighting that S100A12 is likely to be a potential biomarker of therapeutic efficacy and disease progression in coronary heart disease. Future studies of patients with coronary heart disease may provide more evidences supporting that S100 proteins is promising drug target in the prevention and therapy of atherosclerosis. © 2019 Elsevier B.V.

语种： 英文

作者： Zhou, Fan;Huang, Liang;Qu, Shun-Lin;Chao, Ru;Yang, Chen;...

期刊： Clinica Chimica Acta,2019年497:130-136 ISSN：0009-8981

通讯作者： Zhang, Chi

作者机构： [Jiang, Zhi-Sheng; Huang, Liang; Yang, Chen; Zhou, Fan; Qu, Shun-Lin; Chao, Ru; Zhang, Chi] Univ South China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Hunan, Peoples R China.;[Huang, Liang] Univ South China, Sch Med, Dept Operat Surg, Hengyang 421002, Hunan, Peoples R China.;[Zhang, Chi] Univ South China, Hengyang Med Coll, Hunan Int Sci & Technol Cooperat Base Arterioscle, Key Lab Arteriosclerol Hunan Prov,Inst Cardiovasc, 28 Chang Sheng West Rd, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Zhang, Chi] U;Univ South China, Hengyang Med Coll, Hunan Int Sci & Technol Cooperat Base Arterioscle, Key Lab Arteriosclerol Hunan Prov,Inst Cardiovasc, 28 Chang Sheng West Rd, Hengyang 421001, Hunan, Peoples R China.

关键词： Diabetes;Diabetic complications;Extracellular vesicles;miRNAs

摘要： Diabetes and diabetic vascular complications are now the leading cause of death in the world. The effects of traditional medical treatment are usually limited and accompanied by many side effects, such as hypoglycemia, obesity, liver and kidney damage, and gastrointestinal adverse reactions. Thus, it is urgent to explore some new strategies for the treatment of patients with diabetes. Recently, extracellular vesicles have received increased attention because of their emerging roles of cell–to–cell communication under physiological and pathological conditions. In addition, because of their abundant existence in almost all body fluids, as well as their plentiful cargos of bioactive proteins and miRNAs they carry, extracellular vesicles have a strong potential for therapeutic and diagnostic applications in many metabolic diseases, such as obesity and insulin resistance. Here, with the aim of providing the basis for the development of new treatments for diabetes, we review current understanding of extracellular vesicles and the critical roles it has played in the onset and progression of diabetes and diabetic complications. © 2019

语种： 英文

作者： Yu, Hanjie;Li, Xiaojie;Chen, Mengting;Zhang, Fan;Liu, Xiawei;...

期刊： FRONTIERS IN ONCOLOGY,2019年9(JULY):459494 ISSN：2234-943X

通讯作者： Li, Zheng;Xie, Hailong

作者机构： [Du, Haoqi; Li, Zheng; Chen, Wentian; Zhang, Chen; Zhang, Fan; Yu, Hanjie; Liu, Xiawei; Zhang, Jing; Chen, Mengting; Zhang, Kun; Zhong, Yaogang; Yu, Jingmin; Shu, Jian] Northwest Univ, Coll Life Sci, Lab Funct Glyc, Xian, Shaanxi, Peoples R China.;[Li, Xiaojie] 1st Peoples Hosp Chenzhou, Dept Pathol, Chenzhou, Peoples R China.;[Xie, Hailong] Univ South China, Inst Canc Res, Hengyang, Peoples R China.

通讯机构： [Li, Zheng] N;[Xie, Hailong] U;Northwest Univ, Coll Life Sci, Lab Funct Glyc, Xian, Shaanxi, Peoples R China.;Univ South China, Inst Canc Res, Hengyang, Peoples R China.

关键词： Gastric Carcinoma;Glycosylation;Lectin microarray;MALDI-TOF/TOF-MS;LacNAc

摘要： Aberrant glycosylation is not only a feature of malignant cell transformation, but also plays an important role in metastasis. In the present study, an integrated strategy combining the lectin microarrays and lectin cytochemistry was employed to investigate and verify the altered glycopatterns in gastric cancer (GC) cell lines as well as resected tumor specimens from matched tissue sets of 46 GC patients. Subsequently, lectin-mediated affinity capture glycoproteins, and MALDI-TOF/TOF-MS were employed to further acquire precise structural information of the altered glycans. According to the results, the glycopatterns recognized by 10 (e.g., ACA, MAL-I, and ConA) and 3 lectins (PNA, MAL-I, and VVA) showed significantly variations in GC cells and tissue compared to their corresponding controls, respectively. Notably, the relative abundance of Galβ-1,4GlcNAc (LacNAc) recognized by MAL-I exhibited a significant increase in GC cells (p < 0.001) and tissue from patients at stage II and III (p < 0.05), and a significant increase in lymph node positive tumor cases, compared with lymph node negative tumor cases (p < 0.05). More LacNAc contained N-glycans were characterized in tumor sample with advanced stage compared to corresponding control. Moreover, there were 10 neo-LacNAc-contained N-glycans (e.g., m/z 1625.605, 1803.652, and 1914.671) only presented in GC tissue with advanced stage. Among these, six N-glycans were modified with sialic acid or fucose based on LacNAc to form sialylated N-glycans or lewis antigens, respectively. Our results revealed that the aberrant expression of LacNAc is a characteristic of GC, and LacNAc may serve as a scaffold to be further modified with sialic acid or fucose. Our findings provided useful information for us to understand the development of GC.

语种： 英文

作者： Hu, Heng-Jing;Zhang, Chi;Tang, Zhi-Han;Qu, Shun-Lin;Jiang, Zhi-Sheng*

期刊： Biochemical and Biophysical Research Communications,2019年516(3):653-660 ISSN：0006-291X

通讯作者： Jiang, Zhi-Sheng

作者机构： [Jiang, Zhi-Sheng; Hu, Heng-Jing] Univ South China, Dept Cardiol Lab, Affiliated Hosp 1, Hengyang, Hunan, Peoples R China.;[Jiang, Zhi-Sheng; Tang, Zhi-Han; Qu, Shun-Lin; Zhang, Chi] Univ South China, Inst Cardiovasc Dis, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Jiang, Zhi-Sheng; Tang, Zhi-Han; Qu, Shun-Lin; Zhang, Chi] Univ South China, Key Lab Arteriosclerol Hunan Prov, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Jiang, Zhi-Sheng; Hu, Heng-Jing] Univ South China, Postdoctoral Res Stn Basic Med, Hengyang, Hunan, Peoples R China.

通讯机构： [Jiang, Zhi-Sheng] U;Univ South China, Inst Cardiovasc Dis, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Key Lab Arteriosclerol Hunan Prov, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.

关键词： Atrial fibrillation;Pyruvate dehydrogenase kinase-1;Pyruvate dehydrogenase kinase-4;Warburg effect

摘要： Atrial fibrillation (AF) is associated with metabolic stress and induces myocardial fibrosis reconstruction by increasing glycolysis. One goal in the treatment of paroxysmal AF (p-AF) is to improve myocardial fibrosis reconstruction and myocardial metabolic stress caused by the Warburg effect. Adopted male canine that rapid right atrial pacing (RAP) for 6 days to establish a p-AF model. The canines were pre-treated with phenylephrine (PE) or dichloroacetic acid (DCA) before exposure to p-AF or non-p-AF. P-wave duration (P-max), minimum P-wave duration (P-min), P wave dispersion (PWD), atrial effective refractory period (AERP) and AERP dispersion (AERPd) were measured in canine atrial cardiomyocytes. Pyruvate dehydrogenase kinase-1 (PDK-1), PDK-4, lactate dehydrogenase A (LDHA), pyruvate dehydrogenase (PDH), citrate synthase (CS), isocitrate dehydrogenase (IDH), and matrix metalloproteinase 9 (MMP-9) were evaluated by western blotting and reverse transcription polymerase chain reaction (RTPCR), content of adenosine monophosphate (AMP), adenosine triphosphate (ATP), lactic acid and glycogen, and activity of LDHA, PDK-1 and PDK-4 were evaluated by enzyme-linked immunosorbent assay (ELISA), myocardial tissue glycogen content was evaluated by PAS, myocardial fibrosis remodeling was evaluated by hematoxylin and eosin (H&E) and Masson staining. Our findings demonstrated that p-AF increases the Warburg effect-related metabolic stress and myocardial fibrosis remodeling by increasing the expression and activity of PDK-1, PDK-4, and LDHA, content of AMP and lactic acid, and the ratio of AMP/ATP and decreasing the expression of PDH, CS, and IDH, and glycogen content. In addition, p-AF can induce cardiomyocyte fibrosis remodeling and increase MMP-9 expression, and p-AF also increases atrial intracardiac waveform activity by prolonging P-max, P-min, PWD, and AERPd and shortening AERP. PDK isoforms agonists (PE) produce a similar p-AF pathological effect and can produce synergistic effects with p-AF, further increasing Warburg effect-related metabolic stress, myocardial fibrosis remodeling, and atrial intracardiac waveform activity. In contrast, the use of PDK-specific inhibitors (DCA) completely reverses these pathophysiological changes induced by p-AF. We demonstrate that p-AF can induce the Warburg effect in canine atrial cardiomyocytes and significantly improve p-AF-induced metabolic stress, myocardial fibrosis remodeling, and atrial intracardiac waveform activity by inhibiting the Warburg effect. (C) 2019 Elsevier Inc. All rights reserved.

语种： 英文

作者： 周凡;巢汝;杨晨;屈顺林;张弛

期刊： 生命的化学,2019年39(3):510-514 ISSN：1000-1336

作者机构： 南华大学心血管疾病研究所,动脉硬化学湖南省重点实验室,动脉硬化性疾病湖南省国际科技合作创新基地,衡阳421001;[杨晨; 周凡; 张弛; 屈顺林; 巢汝] 南华大学

关键词： 高密度脂蛋白;脓毒症;载脂蛋白A-I

摘要： 脓毒症是指由感染引起的全身炎症反应综合征,由于机体应对感染反应失调而导致多器官功能障碍,常危及生命。研究发现,高密度脂蛋白(high-density lipoprotein, HDL)在调节血管内皮细胞功能和机体免疫中发挥着重要作用,提高循环血HDL水平可以有效预防脓毒性休克的发生。本文论述了 HDL抗脓毒症的潜在机制以及重组高密度脂蛋白应用于脓毒症治疗的前景和面临的挑战。

语种： 中文

作者： 曾勇智;张弛;易光辉

期刊： 吉林医学,2018年39(3):597-599 ISSN：1004-0412

作者机构： 南华大学医学院机能学实验中心,湖南衡阳,421001;[张弛; 易光辉; 曾勇智] 南华大学

关键词： 开放式;医学机能实验;教学模式

摘要： 近年来,我校以“一基三实、一路三建”为发展思路,围绕创建特色鲜明的高水平教学研究型大学为目标,不断完善本科专业创新人才培养方案,深化高校课程教学模式的改革,对于专业核心课程要求全部采用研究型教学模式,倡导问题导向的学习方法,学生变被动接受为主动获取,这就要求学校构建的实践教学体系和内容须体现“以学生为本”的教学理念,学校所提供的实验平台必须是开放式的,以满足学生开展自主学习与实践创新的需要.医学作为一门实践性很强的学科,为了更好地促进资源共享,许多高等医学院校成立了机能学实验中心.

语种： 中文

作者： 黄靓;翁钰杰;张培;龙吟;张弛

期刊： 科教文汇(下旬刊),2018年(30):80-81 ISSN：1672-7894

作者机构： 南华大学外总教研室 湖南·衡阳 421001;南华大学医学院 湖南·衡阳 421001;南华大学机能学实验中心 湖南·衡阳 421001;[张培; 翁钰杰; 张弛; 龙吟; 黄靓] 南华大学

关键词： 标准化病人;培训;考核;问诊

摘要： 标准化病人传入国内已有20多年历史,其培养体系正不断完善,结合文献与个人培训经验,分析标准化病人在招募、培训、考核及录用中的实际流程,探究标准化病人应用的实际意义,以期为临床教学与技能考试提供参考。

语种： 中文

作者： 胡恒境;刘启明;谢忠;周丹;唐志晗;...

期刊： 中国动脉硬化杂志,2018年26(7):678-684 ISSN：1007-3949

作者机构： 南华大学附属第一医院心内科,湖南省衡阳市421001;南华大学心血管疾病研究所动脉硬化学湖南省重点实验室,湖南省衡阳市421001;中南大学湘雅二医院心内科,湖南省长沙市,410000;南华大学附属第一医院心内科,湖南省衡阳市,421001;南华大学心血管疾病研究所动脉硬化学湖南省重点实验室,湖南省衡阳市,421001

关键词： 内脂素;心房颤动;急性ST段抬高型心肌梗死;主要不良心血管事件

摘要： 目的 循环内脂素(visfatin)水平高低与急性ST段抬高型心肌梗死(STEMI)患者的全因死亡发生具有相关性.据此推测,较高的内脂素可能与主要不良心血管事件(MACE)的发生有关,并增加急诊经皮冠状动脉介入治疗(PPCI) STEMI患者心房颤动(AF)的发生率.方法 604例接受PPCI治疗的STEMI患者纳入研究.使用ELISA法测量术前血浆内脂素浓度.随访1年,记录术后AF及MACE发生率,并分析血浆内脂素水平与MACE、新发AF之间的关系.结果 在604例患者中,新发AF42例(6.95％).与术后无AF患者相比,新发AF患者1年MACE发生率升高(45.24％比19.57％,P＜0.05).此外,与血浆低水平内脂素(≤14.5 μg/L)患者相比,血浆高水平内脂素(＞14.5g/L)患者拥有更高的MACE发生率(31.02％比9.56％,P＜0.001)和新发AF率(10.24％比2.94％,P＜0.001).多因素Cox风险回归模型分析显示,血浆内脂素水平为PPCI术后1年新发AF的独立预测因素(HR l.51,95％CI l.03～2.23,P=0.021).结论 在行PPCI的STEMI患者中,血浆内脂素水平高低与MACE的发生有关,并且与新发AF率呈正相关.

语种： 中文

作者： 巢汝;黄靓;屈顺林;张弛

期刊： 生命的化学,2018年38(3):478-482 ISSN：1000-1336

作者机构： [巢汝; 黄靓; 屈顺林; 张弛] 南华大学心血管疾病研究所, 动脉硬化学湖南省重点实验室, 衡阳, 421001

关键词： 巨噬细胞;动脉粥样硬化;能量代谢

摘要： 巨噬细胞通过细胞表型转换与内环境的变化相适应.M1型巨噬细胞具有促炎作用,而M2型巨噬细胞具有抗炎和促纤维化作用.进展的易损动脉粥样硬化斑块内以M1型巨噬细胞为主.糖酵解是M1型巨噬细胞主要的供能途径,伴随细胞内大量活性氧的生成和炎性细胞因子的分泌.而稳定动脉粥样硬化斑块内以M2型巨噬细胞为主,M2型巨噬细胞以氧化磷酸化为主要供能途径,通过抑制巨噬细胞糖酵解,促进巨噬细胞氧化磷酸化,能够减轻动脉粥样硬化病变.本文综述了与巨噬细胞表型转换相适应的细胞能量代谢变化及其对动脉粥样硬化斑块进展和斑块稳定性的影响及其作用机制.

语种： 中文

作者： Fang, Yimin*;Hill, Cristal M.;Darcy, Justin;Reyes-Ordonez, Adriana;Arauz, Edwin;...

期刊： Proceedings of the National Academy of Sciences of the United States of America,2018年115(7):E1495-E1503 ISSN：0027-8424

通讯作者： Fang, Yimin

作者机构： [Hill, Cristal M.; Osland, Jared; Darcy, Justin; McFadden, Samuel; Fang, Yimin; Bartke, Andrzej; Yuan, Rong] Southern Illinois Univ, Dept Internal Med, Sch Med, Springfield, IL 62702 USA.;[Chen, Jie; Arauz, Edwin; Reyes-Ordonez, Adriana] Univ Illinois, Dept Cell & Dev Biol, Urbana, IL 61801 USA.;[Zhang, Chi] Univ South China, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.;[Gao, John] Southern Illinois Univ, Dept Pathol & Lab Med, Sch Med, Springfield, IL 62702 USA.;[Zhang, Tian] Southern Illinois Univ, Dept Surg, Sch Med, Springfield, IL 62702 USA.

通讯机构： [Fang, Yimin] S;Southern Illinois Univ, Dept Internal Med, Sch Med, Springfield, IL 62702 USA.

关键词： mTORC2;rapamycin;longevity;homeostasis;GHR-KO

摘要： It is well documented that inhibition of mTORC1 (defined by Raptor), a complex of mechanistic target of rapamycin (mTOR), extends life span, but less is known about the mechanisms by which mTORC2 (defined by Rictor) impacts longevity. Here, rapamycin (an inhibitor of mTOR) was used in GHR-KO (growth hormone receptor knockout) mice, which have suppressed mTORC1 and up-regulated mTORC2 signaling, to determine the effect of concurrently decreased mTORC1 and mTORC2 signaling on life span. We found that rapamycin extended life span in control normal (N) mice, whereas it had the opposite effect in GHR-KO mice. In the rapamycin-treated GHR-KO mice, mTORC2 signaling was reduced without further inhibition of mTORC1 in the liver, muscle, and s.c. fat. Glucose and lipid homeostasis were impaired, and old GHR-KO mice treated with rapamycin lost functional immune cells and had increased inflammation. In GHR-KO MEF cells, knockdown of Rictor, but not Raptor, decreased mTORC2 signaling. We conclude that drastic reduction of mTORC2 plays important roles in impaired longevity in GHR-KO mice via disruption of whole-body homeostasis. © 2018 National Academy of Sciences.All Rights Reserved.

语种： 英文

作者： 黄靓;赵成;罗玉荣;邹飞燕;张弛

期刊： 西部素质教育,2018年4(08):133-134 ISSN：2095-6401

作者机构： 南华大学外总教研室,湖南 衡阳,421001;南华大学医学院,湖南 衡阳,421001;南华大学机能学实验中心,湖南 衡阳,421001;[罗玉荣; 张弛; 邹飞燕; 赵成; 黄靓] 南华大学

关键词： 临床技能学;专业研讨课;教学计划

摘要： 为了给相关专业开设临床技能学专业研讨课提供新思路,文章首先以南华大学为例,阐述了临床技能学专业研讨课对大二学生从基础医学向临床医学这一过渡阶段起到的重要作用,然后从开设临床技能学研讨课的目的、临床技能学专业研讨课的教学计划和授课方式、学生表现、成绩评定和反馈信息、临床技能学专业研讨课的总结和改进措施等方面对临床技能学专业研讨课进行了分析。

语种： 中文

作者： 黄靓;杨麒玉;王芳;朱梦霞;张弛

期刊： 西部素质教育,2018年4(5):172-173 ISSN：2095-6401

作者机构： 南华大学船山学院医学系,湖南 衡阳,421001;南华大学外总教研室,湖南 衡阳,421001;南华大学临床技能中心,湖南 衡阳,421001;南华大学机能学实验中心,湖南 衡阳,421001;[张弛; 王芳; 朱梦霞; 杨麒玉; 黄靓] 南华大学

关键词： 独立学院;特色为根;应用型医学人才

摘要： 文章通过分析我国独立学院医学生人才培养现状,提出以“特色为根”,探寻应用型医学人才培养模式。具体包括人才培养目标突出特色;根据社会和市场需求,优化专业设置;特色化课程体系的构建;强化实践教学环节;构建具有特色的考核评价体系。

语种： 中文

作者： Fu, Hao;Song, Wenke;Chen, Xuancai;Guo, Tao;Duan, Bin;...

期刊： Molecular and Cellular Biochemistry,2018年437(1-2):143-152 ISSN：0300-8177

通讯作者： Huang, Liang;Zhang, Chi

作者机构： [Duan, Bin; Chen, Xuancai; Wang, Xinxi; Fu, Hao; Tang, Yachun; Guo, Tao] Univ South China, Affiliated Nanhua Hosp, Dept Urol, Hengyang 421002, Hunan, Peoples R China.;[Song, Wenke] Univ South China, Affiliated Nanhua Hosp, Dept Obstet & Gynecol, Hengyang 421002, Hunan, Peoples R China.;[Huang, Liang] Univ South China, Sch Med, Dept Operat Surg, Hengyang 421002, Hunan, Peoples R China.;[Zhang, Chi] Univ South China, Sch Med, Funct Lab Ctr, Dept Pathophisiol, Hengyang 421002, Hunan, Peoples R China.

通讯机构： [Huang, Liang; Zhang, Chi] U;Univ South China, Sch Med, Dept Operat Surg, Hengyang 421002, Hunan, Peoples R China.;Univ South China, Sch Med, Funct Lab Ctr, Dept Pathophisiol, Hengyang 421002, Hunan, Peoples R China.

关键词： MiR-200a;SIRT1;Renal cell carcinoma;Apoptosis

摘要： Accumulating evidence indicates that microRNAs are implicated in tumor initiation and progression through negatively regulating oncogenes or tumor suppressor genes. In the present study, we report that the expression of miR-200a was significantly lower in renal cell carcinoma (RCC) specimens and RCC cell lines. Restoration of miR-200a suppressed cell growth, arrested cell cycle progression, and promoted cell apoptosis in RCC cell lines. We next used qRT-PCR array technology to identify Sirtuin 1 (SIRT1) as one of the downregulated proteins during miR-200a overexpression in 786-O cells. Following a further assay by luciferase reporter system, SIRT1 was validated as a direct target of miR-200a. Moreover, siRNA-mediated knockdown of SIRT1 could partially phenocopy the effects of miR-200a overexpression. In contrast, overexpression of truncated SIRT1 (without an endogenous 3′-UTR) could rescue the effect of miR-200a overexpression on 786-O cells, which suggested that SIRT1 3′-UTR is targeted by miR-200a specifically. These observations provide further evidence for a critical tumor-suppressive role of the miR-200a in RCC in addition to identifying a novel regulatory mechanism, which may contribute to SIRT1 upregulation in RCC. © 2017, Springer Science+Business Media, LLC.

语种： 英文

作者： Hu, Tian;Li, Jincheng;Zhang, Chuhong;Lv, Xiu;Li, Sai;...

期刊： 基因与疾病,2017年4(2):116-122 ISSN：2352-4820

通讯作者： Wen, Meiling;Zuo, Jianhong

作者机构： [Tan, Yixi; Zuo, Jianhong; Lv, Xiu; Wen, Meiling; Zhang, Chuhong; Li, Sai; Yan, Hanxing; Hu, Tian; He, Sha] Univ South China, Affiliated Nanhua Hosp, Hengyang 421001, Hunan, Peoples R China.;[Zuo, Jianhong; Hu, Tian] Univ South China, Sch Med, Hengyang 421001, Hunan, Peoples R China.;[Li, Jincheng] Shaoyang Univ, Med Sch, Shaoyang 422000, Hunan, Peoples R China.;[Lei, Mingsheng] Zhangjiajie City Hosp, Dept Resp & Crit Care Med, Zhangjiajie 427000, Hunan, Peoples R China.

通讯机构： [Wen, Meiling; Zuo, Jianhong] U;Univ South China, Affiliated Nanhua Hosp, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Sch Med, Hengyang 421001, Hunan, Peoples R China.

关键词： Hepatocellular carcinoma;malignant tumors;microRNA-4463;microRNA-6087;miR-4463;Prognostic

摘要： The purpose of this study is to measure the expression of microRNA-4463 and microRNA-6087 between normal persons and patients with hepatocellular carcinoma (HCC), and to clarify the meaning of them in the prognosis evaluation in HCC. Forty-five samples from healthy people and patients, who had been diagnosed with hepatocellular carcinoma before any treatment, were collected to study respectively. Real-time PCR was used to detect the expression of miRNA-4463 and miRNA-6087 in the serum of control group and hepatocellular carcinoma patients. The expression of miR-4463 in the serum of HCC patients was significantly higher than that in control group (P < 0.05), and the expression level was independent of gender, tumor size, cell types, stages, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and HBsAg status (P > 0.05). But there was a significant difference of different level of AFP in HCC (P < 0.05), and the difference between the group of AFP lower than 400 ug/l and the control group is statistically significant (P < 0.05). Besides, the survival time had showed a significant difference at the high and low expression levels (P < 0.05). But the expression level of miRNA-6087 was no difference in HCC and control group. The disorder of miRNA-4463 occurred in HCC, even the AFP level doesn't rises. What's more, patients who get the high level of miRNA-4463 seem to have a shorter survival time. And it contributes great to the prognostic evaluation. This is the first study to illustrate the potential significance of miRNA-4463 in the prognosis in HCC. © 2017 Chongqing Medical University

语种： 英文

作者： 张弛;梁笃;许子宜;吴建群;刘松;...

期刊： 中华创伤骨科杂志,2017年11(7):617-623 ISSN：1007-3949

作者机构： [张弛; 刘松; 王簕; 王钊; 胡汉生] 510145,广州医科大学附属第三医院骨科;[梁笃] 510045,广州市正骨医院骨伤六区;南方医科大学, 广州,510515;[许子宜; 吴建群] 南方医科大学

关键词： 神经肽Y;小鼠;细胞增殖;细胞分化;成骨细胞系细胞

摘要： 目的 探讨神经肽Y(NPY)对小鼠成骨细胞系细胞(MC3T3-E1细胞)成骨分化的生物学效应,并从Wnt信号通路角度探讨其可能的机制. 方法 体外培养MC3T3-E1细胞,将不同浓度的NPY(10-8 mol/L、10-10 mol/L、10-12 tool/L)作用于细胞,在培养的不同时期(1、3、5、7、9 d)使用噻唑蓝法检测细胞增殖情况.成骨诱导条件下将不同浓度的NPY作用于MC3T3-E1细胞,在传代培养的不同时期(4、7、14 d)采用免疫细胞化学、Western blot对MC3T3-T1细胞诱导进行鉴定并确定促成骨效应最适的浓度;成骨诱导4d时将最适浓度NPY、NPY加经典Wnt通路抑制因子(DKK1)、NPY加Y1受体拮抗剂分别作用于细胞,采用Western blot法检测经典Wnt通路相关蛋白(β-catenin和p-GSK-3β)的表达,免疫荧光检测各组β-catenin蛋白核内转移的情况. 结果 与对照组相比,不同浓度的NPY在7、9d时均显著促进MC3T3-E1细胞增殖.成骨诱导条件下,NPY(10-s mol/L)和NPY(10-10 mol/L)浓度组、NPY(10-1o mol/L)和NPY(10-12 mol/L)浓度组分别在4d、14 d时显著促进碱性磷酸酶(ALP)蛋白表达.NPY促成骨效应的最适浓度为10-10 mol/L.在成骨诱导4d时,使用DKK1或Y1受体拮抗剂可以显著抑制NPY(10-10 mol/L)组对ALP蛋白表达的促进作用,虽然β3-catenin和p-GSK-3β蛋白表达未受影响,但是NPY可明显激活β-catenin的核内转移. 结论 NPY促进MC3T3-E1细胞成骨分化过程中ALP的蛋白表达,该效应可能是通过经典Wnt信号通路介导.

语种： 中文

作者： Zhu, Yu-Ning;Fan, Wen-Jing;Zhang, Chi;Guo, Fang;Li, Wei;...

期刊： MOLECULAR MEDICINE REPORTS,2017年15(5):2903-2908 ISSN：1791-2997

通讯作者： Qu, Shun-Lin

作者机构： [Jiang, Zhi-Sheng; Qu, Shun-Lin; Guo, Fang; Li, Wei; Zhang, Chi; Zhu, Yu-Ning; Wang, Yu-Fei; Fan, Wen-Jing] Univ South China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Fan, Wen-Jing] Univ South China, Emergency Dept, Affiliated Hosp 2, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Qu, Shun-Lin] U;Univ South China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.

关键词： Atherosclerosis;Autophagy;Molecular mechanism;Treatment;Vascular

摘要： Atherosclerosis (AS) remains the leading cause for global cardiovascular disease morbidity and mortality, and a major cause of cardiopathy, myocardial infarction and peripheral vascular diseases. Macrophages serve a critical role in atherosclerotic plaque stabilization and rupture, and the selective removal of macrophages may be beneficial in improving plaque stability. Autophagy is a process of self-feeding, during which cytoplasmic proteins or organelles are packaged into vesicles and fused with the lysosome to form an autophagosome. The newly formed autophagosome can degrade internalized proteins, and this process may be used to serve the metabolic and self-renewal requirements of the cell. Autophagy serves an important role in maintaining cell homeostasis and promoting cell survival, and therefore an imbalance in autophagy is closely associated with multiple diseases.

语种： 英文

作者： Lv, Xiu;Li, Jincheng;Zhang, Chuhong;Hu, Tian;Li, Sai;...

期刊： 基因与疾病,2017年4(1):19-24 ISSN：2352-4820

通讯作者： Wen, Meiling;Zuo, Jianhong

作者机构： [Tan, Yixi; Zuo, Jianhong; Lv, Xiu; Wen, Meiling; Zhang, Chuhong; Li, Sai; Yan, Hanxing; Hu, Tian; He, Sha] Univ South China, Affiliated Nanhua Hosp, Hengyang 421001, Hunan, Peoples R China.;[Zuo, Jianhong; Lv, Xiu] Univ South China, Sch Med, Hengyang 421001, Hunan, Peoples R China.;[Li, Jincheng] Shaoyang Univ, Med Sch, Shaoyang 422000, Hunan, Peoples R China.;[Lei, Mingsheng] Zhangjiajie City Hosp, Dept Resp & Crit Care Med, Zhangjiajie 427000, Hunan, Peoples R China.

通讯机构： [Wen, Meiling; Zuo, Jianhong] U;Univ South China, Affiliated Nanhua Hosp, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Sch Med, Hengyang 421001, Hunan, Peoples R China.

关键词： Angiogenesis;HIF;Hypoxia;Metabolism;VHL

摘要： Hypoxia-inducible factor (HIF) is a main heterodimeric transcription factor that regulates the cellular adaptive response to hypoxia by stimulating the transcription of a series of hypoxia-inducible genes. HIF is frequently upregulated in solid tumors, and the overexpression of HIF can promote tumor progression or aggressiveness by blood vessel architecture and altering cellular metabolism. In this review, we focused on the pivotal role of HIF in tumor angiogenesis and energy metabolism. Furthermore, we also emphasized the possibility of HIF pathway as a potential therapeutic target in cancer. © 2016 Chongqing Medical University

语种： 英文

作者： 赵战芝;谭健苗;张弛;易光辉

期刊： 中国高等医学教育,2016年(5):106-107 ISSN：1002-1701

作者机构： 南华大学医学院,湖南衡阳,421001;[赵战芝; 张弛; 易光辉; 谭健苗] 南华大学

关键词： 机能实验学;任务教学法;教学效果

摘要： 目的：探讨任务教学法在机能实验学教学中的应用效果。方法：以2012级临床医学专业部分学生为研究对象，对照组采用传统教学法授课，实验组采用任务教学法授课，以实验成绩和问卷调查评估教学效果。结果：实验组的总成绩和优秀率、学生的学习兴趣、自主学习能力及对教学法的满意度均高于对照组。结论：任务教学法可显著提升机能实验学的教学效果。

语种： 中文

作者： Zhang, Chi;Qu, Shunlin;Wei, Xing;Feng, Yansheng;Zhu, Honglin;...

期刊： CELL STRESS & CHAPERONES,2016年21(2):251-260 ISSN：1355-8145

通讯作者： Xiao, Xianzhong

作者机构： [Wei, Xing; Xiao, Xianzhong; Feng, Yansheng; Wang, Kangkai; Zhang, Chi; Zhu, Honglin; Liu, Meidong; Zhang, Huali; Qu, Shunlin; Liu, Ke] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, 110 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.;[Wei, Xing; Deng, Jia; Zhang, Chi; Qu, Shunlin] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Xiao, Xianzhong] C;Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, 110 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.

关键词： HSP25;SIRT1;p53;Cardiomyocyte apoptosis;Doxorubicin-induced cardiotoxicity

摘要： Heat shock proteins (HSPs) play important roles in cellular stress resistance. Previous reports had already suggested that HSP27 played multiple roles in preventing doxorubicin-induced cardiotoxicity. Although HSP25 might have biological functions similar to its human homolog HSP27, the mechanism of HSP25 is still unclear in doxorubicin-induced cardiomyocyte apoptosis. To investigate HSP25 biological function on doxorubicin-induced apoptosis, flow cytometry was employed to analyze cell apoptosis in over-expressing HSP25 H9c2 cells in presence of doxorubicin. Unexpectedly, the H9c2 cells of over-expressing HSP25 have no protective effect on doxorubicin-induced apoptosis. Moreover, no detectable interactions were detected by coimmunoprecipitation between HSP25 and cytochrome c, and HSP25 over-expression failed in preventing cytochrome c release induced by doxorubicin. However, down-regulation of endogenous HSP25 by a specific small hairpin RNA aggravates apoptosis in H9c2 cells. Subsequent studies found that HSP25, but not HSP90, HSP70, and HSP20, interacted with SIRT1. Knockdown of HSP25 decreased the interaction between SIRT1 and p53, leading to increased p53 acetylation on K379, up-regulated pro-apoptotic Bax protein expression, induced cytochrome c release, and triggered caspase-3 and caspase-9 activation. These findings indicated a novel mechanism by which HSP25 regulated p53 acetylation through dissociation of SIRT1 from p53 in doxorubicin-induced H9c2 cell apoptosis. © 2015, Cell Stress Society International.

语种： 英文

作者： Darcy, Justin*;McFadden, Samuel;Fang, Yimin;Huber, Joshua A.;Zhang, Chi;...

期刊： ENDOCRINOLOGY,2016年157(12):4744-4753 ISSN：0013-7227

通讯作者： Darcy, Justin

作者机构： [Fang, Yimin; Zhang, Chi; Bartke, Andrzej; Huber, Joshua A.; Darcy, Justin; McFadden, Samuel] Southern Illinois Univ, Sch Med, Dept Internal Med, Geriatr Res, Springfield, IL 62702 USA.;[Bartke, Andrzej; Darcy, Justin] Southern Illinois Univ, Sch Med, Dept Med Microbiol Immunol & Cell Biol, Springfield, IL 62702 USA.;[Zhang, Chi] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Sun, Liou Y.] Univ Alabama Birmingham, Dept Biol, Birmingham, AL 35294 USA.;[Darcy, Justin] Southern Illinois Univ, Sch Med, Dept Internal Med, POB 19628, Springfield, IL 62702 USA.

通讯机构： [Darcy, Justin] S;Southern Illinois Univ, Sch Med, Dept Internal Med, POB 19628, Springfield, IL 62702 USA.

摘要： Ames dwarf mice (Prop1df/df) are long-lived due to a loss of function mutation, resulting in deficiency of GH, TSH, and prolactin. Along with a marked extension of longevity, Ames dwarf mice have improved energy metabolism as measured by an increase in their oxygen consumption and heat production, as well as a decrease in their respiratory quotient.Alongwith alterations in energy metabolism, Ames dwarf mice have a lower core body temperature. Moreover, Ames dwarf mice have functionally altered epididymal white adipose tissue (WAT) that improves, rather than impairs, their insulin sensitivity due to a shift from pro-to anti-inflammatory cytokine secretion. Given the unique phenotype of Ames dwarf epididymal WAT, their improved energy metabolism, and lower core body temperature, we hypothesized that Ames dwarf brown adipose tissue (BAT) may function differently from that of their normal littermates. Here we use histology and RT-PCR to demonstrate that Ames dwarf mice have enhanced BAT function. We also use interscapular BAT removal to demonstrate that BAT is necessary for Ames dwarf energy metabolism and thermogenesis, whereas it is less important for their normal littermates. Furthermore, we show that Ames dwarf mice are able to compensate for loss of interscapular BAT by using their WAT depots as an energy source. These findings demonstrate enhanced BAT function in animals withGHand thyroid hormone deficiencies, chronic reduction of body temperature, and remarkably extended longevity. Copyright © 2016 by the Endocrine Society.

语种： 英文