期刊论文

Neuropeptide Y stimulates osteoblastic differentiation of murine MC3T3-E1 cells related to activated Wnt signaling in vitro

中文

中华创伤骨科杂志

Chinese Journal of Orthopaedic Trauma

1007-3949

2017

11

7

617-623

10.3760/cma.j.issn.1671-7600.2017.07.011

国家自然科学基金(31570980);广州市医药卫生科技项目(20141A010082).National Natural Science Foundation of China(31570980);Medical Science Program of Guangzhou(20141A010082)

本校为其他机构

目的 探讨神经肽Y(NPY)对小鼠成骨细胞系细胞(MC3T3-E1细胞)成骨分化的生物学效应,并从Wnt信号通路角度探讨其可能的机制. 方法 体外培养MC3T3-E1细胞,将不同浓度的NPY(10-8 mol/L、10-10 mol/L、10-12 tool/L)作用于细胞,在培养的不同时期(1、3、5、7、9 d)使用噻唑蓝法检测细胞增殖情况.成骨诱导条件下将不同浓度的NPY作用于MC3T3-E1细胞,在传代培养的不同时期(4、7、14 d)采用免疫细胞化学、Western blot对MC3T3-T1细胞诱导进行鉴定并确定促成骨效应最适的浓度;成骨诱导4d时将最适浓度NPY、NPY加经典Wnt通路抑制因子(DKK1)、NPY加Y1受体拮抗剂分别作用于细胞,采用Western blot法检测经典Wnt通...

Objective To investigate the effect of neuropeptide Y （NPY） on the osteoblastic dif- ferentiation of routine MC3T3-E1 cells and its mechanism related to the Wnt signaling pathway. Methods The routine MC3T3-E1 cells were divided into 4 groups according to the stimulators added： phosphate buffered saline （PBS） （control） and different concentrations of NPY （10^-8 mol/L, 10^-10 mol/L and 10^-12 mol/L）. The cellular proliferation was detected with MTT assay after 1, 3, 5, 7 and 9 days. The cells were identified with cell immunochemistry and...