期刊论文

中文

中国药理学通报

1001-1978

2014

30

1

104-108

10.3969/j.issn.1001-1978.2014.01.023

11C1093:湖南省教育厅科学研究项目 11C1095:湖南省教育厅科学研究项目

本校为第一机构

目的 研究新型褪黑素受体激动剂Neu-P11对心肌细胞缺氧/复氧损伤的作用及分子机制.方法 实验分为对照组、缺氧/复氧模型组(H/R组)、H/R+Neu-P11组、H/R+Compound C组、H/R+Neu-P11+Compound C组.构建H9c2心肌细胞缺氧/复氧模型,检测各组心肌细胞凋亡情况,细胞培养液中乳酸脱氢酶(LDH)、肌酸激酶(CK)、SOD活性及MDA含量及心肌细胞AMPK的活性.结果 与缺氧/复氧组细胞相比,Neu-P11预处理组细胞凋亡率明显减少,CK、LDH、MDA含量明显下降,SOD活性增加,心肌细胞AMPK活性明显增高,而AMPK特异性抑制剂Compound C可以阻断Neu-P11的保护作用.结论 Neu-P11能够减轻心肌细胞缺氧/复氧损伤,这一保护作用与激活...

Aim: To study the protective effects of Neu-P11 on hypoxia/reoxygenation (H/R) injury in H9c2 myocardial cells. Methods: The hypoxia/reoxygen (H/R) injury cardiomyocyte model was established in H9c2 myocardial cell strain. The cells were randomly divided into five groups; control group, H/R group, H/R + Neu-P11 group, H/R + Neu-P11 + Compound C group and H/R + Compound C group. Cell apoptosis of cardiomyocytes was measured by flow cytometry. The activity of lactate dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD) and the malondialdehyde (MDA) contents were assayed respecti...