作者机构：
[Axel Koenies] Max-Planck-Institut fur Plasmaphysik Teilinstitut Greifswald, Greifswald, Deutschland, GERMANY;[Jinjia Cao] School of Nuclear Science and Technology, University of South China, Hengyang 421001, CHINA;[Ralf Kleiber] Max-Planck-Institut fur Plasmaphysik Teilinstitut Greifswald, Wendelsteinstrasse 1, 17491 Greifswald, Greifswald, 17491, GERMANY
摘要：
We calculate Alfv\'en eigenmodes within a magnetic island (MiAE) 
 which have been conjectured over a decade ago. Starting from 
 a cylindrical plasma equilibrium, we calculate the complete metric of 
 the island interior assuming an iota profile with a constant shear for 
 Wendelstein 7-X (W7-X)
 parameters. Then, we solve the
 resulting magneto-hydrodynamic (MHD) equations inside the island
 optionally considering finite 
 Larmor radius (FLR) corrections. 
 
 We find various eigenmodes in the lowest gaps for $n=0$. 
 The eigenmode with the lowest frequency shows a weakly non-linear dependence on the island width.
 This non-linear behaviour and the appearance of odd and 
 even modes deviates qualitatively from an earlier frequency estimate.
期刊：
Journal of Biological Chemistry,2024年300(3):105721 ISSN：0021-9258
通讯作者：
Wang, Yugang
作者机构：
[Zeng, Xiao; Zhou, Runxin; Wang, Yu; Tong, Fuqiang; Guo, Dingyuan; Yin, Sibi; Wang, Yugang] Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China;[Yu, Weixing] Department of Biochemistry and Molecular Biology, College of Basic Medicine, Jining Medical University, Jining, 272067, China;[Jiang, Li] Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, China, 430060;[Jiang, Li] Department of Neurology, The Affiliated Nanhua Hospital, University of South China, Hengyang, 421001, China;[He, Leya] Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China
通讯机构：
[Yugang Wang] D;Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China<&wdkj&>Cell Architecture Research Center, Huazhong University of Science and Technology, Wuhan, Hubei, China
关键词：
histone sulfation;regulation of gene transcription;hypoxia;glycolysis;PDK1
摘要：
Histone H3 tyrosine-99 sulfation (H3Y99sulf) is a recently identified histone mark that can cross-talk with H4R3me2a to regulate gene transcription, but its role in cancer biology is less studied. Here, we report that H3Y99sulf is a cancer-associated histone mark that can mediate hepatocellular carcinoma (HCC) cells responding to hypoxia. Hypoxia-stimulated SNAIL pathway elevates the expression of PAPSS2, which serves as a source of adenosine 3′-phosphate 5′-phos-phosulfate for histone sulfation and results in upregulation of H3Y99sulf. The transcription factor TDRD3 is the downstream effector of H3Y99sulf-H4R3me2a axis in HCC. It reads and co-localizes with the H3Y99sulf-H4R3me2a dual mark in the promoter regions of HIF1A and PDK1 to regulate gene transcription. Depletion of SULT1B1 can effectively reduce the occurrence of H3Y99sulf-H4R3me2a-TDRD3 axis in gene promoter regions and lead to downregulation of targeted gene transcription. Hypoxia-inducible factor 1-alpha and PDK1 are master regulators for hypoxic responses and cancer metabolism. Disruption of the H3Y99sulf-H4R3me2a-TDRD3 axis can inhibit the expression and functions of hypoxia-inducible factor 1-alpha and PDK1, resulting in suppressed proliferation, tumor growth, and survival of HCC cells suffering hypoxia stress. The present study extends the regulatory and functional mechanisms of H3Y99sulf and improves our understanding of its role in cancer biology.
作者机构：
[Li, Kai; Tan, Fenghua; Chen, Liujie; Li, Jia; Hu, Zheng; Zeng, Jianling; Qu, Jiayao; Duan, Lili; Liang, Xinquan] Translational Medicine Institute, the First People's Hospital of Chenzhou, Hengyang Medical School, University of South China, Chenzhou 423000, China;[Tan, Fenghua; Chen, Liujie; Li, Jia; Hu, Zheng; Zeng, Jianling; Duan, Lili; Liang, Xinquan] The First Affiliated Hospital of Xiangnan University, Chenzhou 423000, China;[Li, Kai] National Engineering Research Center of Personalized Diagnostic and Therapeutic Technology, Hunan University of Chinese Medicine, Changsha 410208, China;[Luo, Dixian] Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen 518000, China;[Hu, Zheng] National & Local Joint Engineering Laboratory for High-through Molecular Diagnosis Technology, the First People's Hospital of Chenzhou, Chenzhou 423000, China
摘要：
The BCR-ABL fusion gene, formed by the fusion of the breakpoint cluster region protein ( BCR) and the Abl Oncogene 1, Receptor Tyrosine Kinase ( ABL) genes, encodes the BCR-ABL oncoprotein, which plays a crucial role in leukemogenesis. Current therapies have limited efficacy in patients with chronic myeloid leukemia (CML) because of drug resistance or disease relapse. Identification of novel strategies to treat CML is essential. This study aims to explore the efficiency of novel CRISPR-associated protein 9 (Cas9)/dual-single guide RNA (sgRNA)-mediated disruption of the BCR-ABL fusion gene by targeting BCR and c-ABL introns. A co-expression vector for Cas9 green fluorescent protein (GFP)/dual-BA-sgRNA targeting BCR and c-ABL introns is constructed to produce lentivirus to affect BCR-ABL expression in CML cells. The effects of dual-sgRNA virus-mediated disruption of BCR-ABL are analyzed via the use of a genomic sequence and at the protein expression level. Cell proliferation, cell clonogenic ability, and cell apoptosis are assessed after dual sgRNA virus infection, and phosphorylated BCR-ABL and its downstream signaling molecules are detected. These effects are further confirmed in a CML mouse model via tail vein injection of Cas9-GFP/dual-BA-sgRNA virus-infected cells and in primary cells isolated from patients with CML. Cas9-GFP/dual-BA-sgRNA efficiently disrupts BCR-ABL at the genomic sequence and gene expression levels in leukemia cells, leading to blockade of the BCR-ABL tyrosine kinase signaling pathway and disruption of its downstream molecules, followed by cell proliferation inhibition and cell apoptosis induction. This method prolongs the lifespan of CML model mice. Furthermore, the effect is confirmed in primary cells derived from patients with CML.
作者机构：
[Chen, Alex F.; Zhang, Zhen; Yu, Fan] Cent South Univ, Dept Cardiol, Xiangya Hosp 3, Changsha, Peoples R China.;[Yu, Fan] Zhejiang Univ, Res Ctr Life Sci & Human Hlth, Binjiang Inst, Hangzhou, Zhejiang, Peoples R China.;[Leng, Yiping] Univ South China, Affiliated Changsha Cent Hosp, Res Ctr PhaseClin Trials 1, Hengyang Med Sch, Changsha, Hunan, Peoples R China.;[Chen, Alex F.] Shanghai Jiao Tong Univ, Inst Cardiovasc Dev & Regenerat Med, Dept Cardiol, Sch Med,Xinhua Hosp, 1665 Kongjiang Rd, Shanghai 200092, Peoples R China.
通讯机构：
[Chen, AF ] C;Cent South Univ, Dept Cardiol, Xiangya Hosp 3, Changsha, Peoples R China.;Shanghai Jiao Tong Univ, Inst Cardiovasc Dev & Regenerat Med, Dept Cardiol, Sch Med,Xinhua Hosp, 1665 Kongjiang Rd, Shanghai 200092, Peoples R China.
关键词：
GSDMD;O-GlcNAc;Sepsis;Endothelial;Pyroptosis
摘要：
OBJECTIVE: Increased O-linked β-N-acetylglucosamine (O-GlcNAc) stimulation has been reported to protect against sepsis associated mortality and cardiovascular derangement. Previous studies, including our own research, have indicated that gasdermin-D(GSDMD)-mediated endothelial cells pyroptosis contributes to sepsis-associated endothelial injury. This study explored the functions and mechanisms of O-GlcNAc modification on lipopolysaccharide (LPS)-induced pyroptosis and its effects on the function of GSDMD. METHODS: A LPS-induced septic mouse model administrated with O-GlcNAcase (OGA) inhibitor thiamet-G (TMG) was used to assess the effects of O-GlcNAcylation on sepsis-associated vascular dysfunction and pyroptosis. We conducted experiments on human umbilical vein endothelial cells (HUVECs) by challenging them with LPS and TMG to investigate the impact of O-GlcNAcylation on endothelial cell pyroptosis and implications of GSDMD. Additionally, we identified potential O-GlcNAcylation sites in GSDMD by utilizing four public O-GlcNAcylation site prediction database, and these sites were ultimately established through gene mutation. RESULTS: Septic mice with increased O-GlcNAc stimulation exhibited reduced endothelial injury, GSDMD cleavage (a marker of pyroptosis). O-GlcNAc modification of GSDMD mitigates LPS-induced pyroptosis in endothelial cells by preventing its interaction with caspase-11 (a human homologous of caspases-4/5). We also identified GSDMD Serine 338 (S338) as a novel site of O-GlcNAc modification, leading to decreased association with caspases-4 in HEK293T cells. CONCLUSIONS: Our findings identified a novel post-translational modification of GSDMD and elucidated the O-GlcNAcylation of GSDMD inhibits LPS-induced endothelial injury, suggesting that O-GlcNAc modification-based treatments could serve as potential interventions for sepsis-associated vascular endothelial injury.
摘要：
Objectives: This study aimed to evaluate the therapeutic effects and mechanism of genistein on dextran sulfate sodium-induced mouse model of ulcerative colitis (UC).Materials and Methods: A DSS-induced mouse model for UC was used in this study. Mice were then treated with genistein or a combination of genistein and the G protein-coupled estrogen receptor (GPER) antagonist G15. Colon length, disease activity index (DAI), spleen index (SI), histopathological alterations, and integrity of the colonic barrier were evaluated. The expression of inflammatory cytokines and antioxidant capacity were detected. Populations of T helper 17 cells (Th17) and Regulatory T cells (Treg) in the mesenteric lymph nodes (MLN) were analyzed. The expression of a transcription factor for Th17 and Treg in the colonic tissues was detected.Results: Genistein treatment significantly alleviated DSS-induced colitis, summarized as increased colonic length, decreased DAI, SI, improved histopathological alterations, and colonic barrier integrity. Genistein treatment restrained pro-inflammatory cytokines and oxidative enzyme release while promoting anti-inflammatory and anti-oxidative enzyme release. Flow cytometry indicated that genistein significantly reduced the Th17 population while boosting Treg populations. Furthermore, genistein inhibited the expression of transcription factors associated with Th17 and promoted the expression of transcription factors associated with Treg in the colonic tissue. Intriguingly, these observed effects of genistein were abolished when UC mice were treated with a combination of genistein and GPER antagonist G15.Conclusion: This study suggests that genistein is potent in protecting against DSS-induced colonic injuries by rebalancing Th17/Treg and that GPER may be a vital target for genistein-mediated immunomodulatory effects in UC.
期刊：
FRONTIERS IN SURGERY,2024年11:1351511 ISSN：2296-875X
作者机构：
The First Affiliated Hospital, University of South China, Hengyang, 421001, Hunan Province, China
关键词：
Full-endoscopic lumbar discectomy;Microsurgery;Disc herniation;clinical efficacy;Posterior apophyseal ring fracture
摘要：
The objective of this study was to evaluate the mid-term efficacy of full-endoscopic lumbar discectomy for patients with posterior apophyseal ring fracture (PARF) combined with lumbar disc herniation (LDH). Methods: Data from patients with PARF combined with LDH who were admitted to our hospital from 2015 to 2020 were reviewed. A total of 42 patients (29 males and 13 females) met the inclusion criteria. Among them, 15 had type I PARF, 20 had type II PARF, and 7 had type III PARF. The mean age was 34.5±2.6 years, and the mean follow-up time was approximately 5.5 years. A total of 30 patients with type I/II PARF were treated with transforaminal approach endoscopy, 5 patients with type I/II PARF who could not be treated with transforaminal approach endoscopy and all patients with type III PARF were treated with interlaminar approach endoscopy. Results: All patients successfully underwent the operation without serious complications. Four patients had unstable vital signs during the operation, and 3 patients had dural rupture during the operation. Four patients had transient neurological symptoms after the operation; three of these patients reported postoperative low back and leg pain, and 1 patient reported postoperative numbness in the perineal area, all of which improved after conservative treatment. The patients' mean postoperative scores on the visual analog scale and the Oswestry Disability Index were significantly improved compared with the preoperative scores. According to the modified MacNab criteria, 27 patients had excellent surgical efficacy, 9 patients had good surgical efficacy, 3 patients had fair surgical efficacy, only 1 patient had poor surgical efficacy. The patients with fair or poor efficacy had more serious neurological dysfunction before surgery. Conclusion: As total spinal endoscopy technology has progressed, it has gradually become the treatment of choice for patients with PARF combined with LDH due to its advantages of safety and minimal invasiveness. However, the technique is difficult for young surgeons due to the small surgical field and operating channel as well as the ambiguous anatomical structure under the mirror. To achieve good surgical efficacy, a longer learning cycle is needed, and more care and patience are needed.
摘要：
To explore the influence of lithology on the failure behavior of layered tunnel, true triaxial compression experiments were undertaken on cubical phyllite and yellow sandstone samples containing a "D" shaped hole. The failure progress of the hole sidewalls was monitored and captured using a miniature camera. The results reveal that the initial vertical failure stress of the phyllite samples presents a "U" shaped change as the bedding angle increases. At the bedding angle of 45 degrees, compared with the initial vertical failure stress of the yellow sandstone tunnel, that of the phyllite tunnel is lower, resulting in larger rock fragments and deeper V-shaped grooves. The failure pattern of the phyllite tunnel is primarily manifested as extensive shear sliding failure, and the failure is more severe. The failure of the yellow sandstone tunnel is primarily characterized by the sequential laminar fracturing along the maximum principal stress direction, predominantly manifesting as tensile failure. The primary factors influencing the failure of two types of layered rocks are the significant variations in the clay mineral content within the rocks. For the phyllite, it contains nearly one-third of montmorillonite (a clay mineral). This results in the formation of weak bedding planes within surrounding rocks, which induces shear slip failures along these bedding planes. In contrast, the yellow sandstone has a lower clay mineral content, leading to the absence of distinct weak bedding planes within the surrounding rock. In this case, bedding planes present ignorable effect on the surrounding rock.
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,2024年1870(3):167050 ISSN：0925-4439
通讯作者：
Wei Huang
作者机构：
[Hu, Ting; Song, Zhihao; Huang, Wei; Liu, Qing; Li, Haoyu] Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, China;[Hu, Ting; Song, Zhihao; Liu, Qing; Li, Haoyu] Institute of Skull Base Surgery and Neurooncology at Hunan Province, Changsha 410008, China;[Hu, Ting; Song, Zhihao; Liu, Qing] National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China;[Zeng, Chong] Department of Medicine, The Seventh Affiliated Hospital, Hengyang Medical School, University of South China, Changsha 410119, China;[Chen, Si] National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China
通讯机构：
[Wei Huang] D;Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, China<&wdkj&>National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China<&wdkj&>Research Center of Carcinogenesis and Targeted Therapy, Xiangya Hospital, Central South University, Changsha 410008, China
摘要：
HNRNPA2B1 and HNRNPR stabilize ASCL1 mRNA in neuroblastoma, but whether their regulatory effects depend on m6A modification and whether their function involves ASCL1 remain unknown. This study investigated the m6A-dependent binding of HNRNPA2B1 and HNRNPR to ASCL1 and subsequent regulation, as well as the expression, clinical significance, and function of HNRNPA2B1 and HNRNPR in neuroblastoma. We revealed that METTL14 mediated ASCL1 m6A modification to stabilize ASCL1. HNRNPA2B1 and HNRNPR significantly enriched ASCL1 mRNA by binding to the 5' and 3' untranslated regions, respectively, and METTL14 knockdown reduced this enrichment. Mutations in m6A sites in the untranslated regions of ASCL1 mRNA considerably decreased probe capacity to engage HNRNPA2B1 and HNRNPR. HNRNPR interacts with IGF2BP1, and knocking down either impaired binding to ASCL1 mRNA. HNRNPA2B1 and HNRNPR knockdown suppressed neuroblastoma cell growth and invasion, while ASCL1 overexpression restored these effects. The high HNRNPA2B1 and HNRNPR expression in neuroblastoma correlated with ASCL1 expression. Thus, HNRNPA2B1 and HNRNPR bind and stabilize ASCL1 mRNA in an m6A-dependent manner to promote neuroblastoma progression. This study not only discovered a new mechanism underlying the high ASCL1 expression in neuroblastoma but also identified the HNRNPA2B1/HNRNPR/ASCL1 axis as a promising target for inhibiting neuroblastoma progression.
摘要：
BACKGROUND: With the burgeoning advancements in disease modeling, drug development, and precision medicine, organ-on-a-chip has risen to the forefront of biomedical research. Specifically in tumor research, this technology has exhibited exceptional potential in elucidating the dynamics of metastasis within the tumor microenvironment. Recognizing the significance of this field, our study aims to provide a comprehensive bibliometric analysis of global scientific contributions related to organ-on-a-chip. METHODS: Publications pertaining to organ-on-a-chip from 2014 to 2023 were retrieved at the Web of Science Core Collection database. Rigorous analyses of 2305 articles were conducted using tools including VOSviewer, CiteSpace, and R-bibliometrix. RESULTS: Over the 10-year span, global publications exhibited a consistent uptrend, anticipating continued growth. The United States and China were identified as dominant contributors, characterized by strong collaborative networks and substantial research investments. Predominant institutions encompass Harvard University, MIT, and the Chinese Academy of Sciences. Leading figures in the domain, such as Dr. Donald Ingber and Dr. Yu Shrike Zhang, emerge as pivotal collaboration prospects. Lab on a Chip, Micromachines, and Frontiers in Bioengineering and Biotechnology were the principal publishing journals. Pertinent keywords encompassed Microfluidic, Microphysiological System, Tissue Engineering, Organoid, In Vitro, Drug Screening, Hydrogel, Tumor Microenvironment, and Bioprinting. Emerging research avenues were identified as "Tumor Microenvironment and Metastasis," "Application of organ-on-a-chip in drug discovery and testing" and "Advancements in personalized medicine applications". CONCLUSION: The organ-on-a-chip domain has demonstrated a transformative impact on understanding disease mechanisms and drug interactions, particularly within the tumor microenvironment. This bibliometric analysis underscores the ever-increasing importance of this field, guiding researchers and clinicians towards potential collaborative avenues and research directions.
作者机构：
[Lu, Jibu; Song, Cailu; Xie, Xiaoming; Liu, Lingrui; Tang, HL; Tang, Hailin; Mo, Yunxian; Wu, Song] Sun Yat Sen Univ, Canc Ctr, Guangdong Prov Clin Res Ctr Canc, State Key Lab Oncol South China, Guangzhou 510060, Peoples R China.;[Li, YH; Zhu, Hongbo; Li, Yuehua; Xie, Liming] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Hengyang, Peoples R China.;[Wu, Feiyue] Guizhou Prov Peoples Hosp, Guiyang, Peoples R China.;[Lin, Huan; Tang, Hailin] Guangzhou Med Univ, Affiliated TCM Hosp, Guangzhou, Peoples R China.
通讯机构：
[Tang, HL ] S;[Li, YH ] U;[Lin, H ] G;Sun Yat Sen Univ, Canc Ctr, Guangdong Prov Clin Res Ctr Canc, State Key Lab Oncol South China, Guangzhou 510060, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Hengyang, Peoples R China.
关键词：
Autophagy;Circular RNAs;Competitive endogenous RNAs;Triple-negative breast cancer;circKIF4A
摘要：
Among patients with triple-negative breast cancer (TNBC), distant metastasis is the leading cause of death. Our previous studies have shown that TNBC progression is greatly facilitated by circKIF4A, but uncertainty remains regarding its role in TNBC brain metastasis and the molecular mechanism. In this study, we found notable upregulation of circKIF4A in TNBC cell lines and brain metastases. Inhibition of circKIF4A impaired the ability of TNBC to proliferate, migrate, and cause brain metastasis. Luciferase reporter assays confirmed that circKIF4A competed for binding to miR-637 with STAT3 3' UTR. Western blot analysis revealed that inhibition of circKIF4A decreased STAT3 and p62 expression, while increased the LC3B-II/LC3B-I ratio and the expression of Beclin, indicating that downregulation of circKIF4A induced autophagy by competing with STAT3 for binding to miR-637. By employing a competitive endogenous RNA (ceRNA) mechanism, the circKIF4A-miR-637-STAT3 axis coordinates brain metastasis in TNBC. circKIF4A can therefore be used as a prognostic biomarker for brain metastasis in TNBC and as a therapeutic target.
期刊：
European Journal of Medicinal Chemistry,2024年268:116274 ISSN：0223-5234
通讯作者：
Yong Guo
作者机构：
[Yang, Ruige; Guo, Yong; Xu, Ting] Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan Province, China;[Yang, Ruige] School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, Henan Province, China;[Zhang, Miaomiao; Cheng, Wanqing; Huang, Meijuan; Liu, Jifeng; Qin, Shangshang] School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, Henan Province, China;[Guo, Yong] School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, Henan Province, China. Electronic address: guoyong_122@163.com
通讯机构：
[Yong Guo] H;Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan Province, China<&wdkj&>School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, Henan Province, China
摘要：
Methicillin-resistant Staphylococcus aureus (MRSA) is a widespread pathogen causing clinical infections and is multi-resistant to many antibiotics, making it urgent need to develop novel antibacterials to combat MRSA. Here, a series of novel isoxanthohumol-amine conjugates were synthesized as antibacterials. After bioactivity evaluation, a compound E2 was obtained, which showed excellent antibacterial activity against S. aureus and clinical MRSA isolates (MICs=0.25-1μg/mL), superior to vancomycin, and with negligible hemolysis and good membrane selectivity. Additionally, E2 exhibited fast bacterial killing, less susceptible to resistance, relatively low cytotoxicity, and good plasma stability. Mechanism investigation revealed that E2 can disrupt bacterial membranes by specifically binding to phosphatidylglycerol on the bacterial membrane, thus causing elevated intracellular ROS and leakage of DNA and proteins, and ultimately killing bacteria. Noticeably, E2 displayed a good in vivo safety profile and better in vivo therapeutic efficacy than the same dose of vancomycin, allowing it to be a potential antibacterial to conquer MRSA infections.
作者机构：
[Wen, S P; Hou, G Y; Li, Xiaoyu; Liang, H; Dai, H L; Yuan, C Z; Zhao, G; Xu, W; Xiao, S Y; Zhao, Ling; Ma, Q M; Lou, X C; Hou, X T; Zhang, P; Liu, K; Tang, G Y; Batozskaya, V; Liu, P L; Wang, H P; Cao, G F; Sun, H K; Yan, X Q; Wang, Y F; Zhang, Jiawei; Kiuchi, R; Shi, R S; Wang, Y Q; Sun, Y Z; Zhang, J Z; Zhang, J W; Zhang, J Y; Zhang, Z H; Wang, Yaqian; K, X; Liu, C X; Mo, X H; Hu, Y; Hu, T; Song, W M; Zhu, Z A; Wu, Z; Zheng, W J; Chang, J F; Yuan, Y; Deng, Z Y; Yang, Yifan; Lu, J G; Yu, G; Hu, H M; Zheng, J P; Zeng, Y J; Ma, R Q; Ouyang, Q; Fang, W X; Liu, Huanhuan; Chen, T; Yuan, S C; Fu, Y W; Rong, G; Chen, G; Ding, B; Liu, B J; Zhu, K J; Hou, Z L; Ablikim, M; Xu, C F; Cai, X; Li, L K; Yang, Tao; Li, L J; Ma, H L; Qiu, J F; Zhang, Shuihan; Liu, Fang; Ma, X Y; Lu, Y P; Heng, Y K; Chang, W L; Qin, Z H; Fang, S S; Wang, B; Sun, S S; Wang, K; Ping, R G; Du, M C; Wang, Z; Liu, H M; Liao, Y P; Mao, Z P; Yin, J H; Yu, B X; Gong, W X; Lu, Z H; Chen, M L; Li, Ke; Zhu, K; Ji, X L; Zhang, B X; Ji, X B; Zhang, B L; Guan, C Y; Jing, M Q; Li, G; Shi, J Y; Li, F; Lin, T; Yuan, X Q; Zhang, A Q; Li, H B; Lu, X L; Xu, G F; Chen, X T; Yang, H X; Gu, M H; Zhang, Yao; Wang, Z Y; Zhang, Y H; Zou, J H; Wu, L H; Wu, L J; Zhang, H Q; Zhou, L P; Chen, H S; Shao, L G; Fu, C D; Zhao, J Y; Zhao, J Z; Xing, T Y; Li, W D; Li, W G; He, K L; Ma, M M; Qi, F Z; Qian, S; Yang, Y X; Zhang, X M; Zhang, H Y; Zhao, Y B; Chen, Y B; Sun, G X; Wang, L L; Ning, Z; Huang, Y P; Wang, Meng; Ma, J L; Dong, L Y; Luo, X L; Jiang, X S; Fang, Y; Fang, J; Ye, M; Shen, X Y; Dong, M Y; Cao, N; Miao, H; Shi, X; Ji, Q; Wu, J F; Liu, Z A; Shen, H F; Sun, T; Xie, Y G; Dong, J; Liu, J Y] Institute of High Energy Physics, Beijing 100049, People's Republic of China;[Achasov, M N; Muchnoi, N Yu; Nikolaev, I B] Budker Institute of Nuclear Physics SB RAS (BINP), Novosibirsk 630090, Russia;[Adlarson, P; Schoenning, K; Thoren, V; Kupsc, A; Wolke, M; Johansson, T] Uppsala University, Box 516, SE-75120 Uppsala, Sweden;[Li, D M; Liu, Y; Zhang, J; Du, S X; Yan, W C; Zhang, Q Y; Ai, X C; Zhao, S J; Ke, B C; Zhang, Y T] Zhengzhou University, Zhengzhou 450001, People's Republic of China;[Redmer, C F; Hüsken, N; Schelhaas, Y; Lenz, T; Lellmann, M; Stieler, F; Leithoff, H; Muskalla, J; Gradl, W; Aliberti, R; Heinz, C H; Plura, S; Berger, N; Denig, A] Johannes Gutenberg University of Mainz, Johann-Joachim-Becher-Weg 45, D-55099 Mainz, Germany
摘要：
Based on data samples collected with the BESIII detector at the BEPCII collider, the process e^{+}e^{-}→Σ^{+}Σ[over ¯]^{-} is studied at center-of-mass energies sqrt[s]=2.3960, 2.6454, and 2.9000GeV. Using a fully differential angular description of the final state particles, both the relative magnitude and phase information of the Σ^{+} electromagnetic form factors in the timelike region are extracted. The relative phase between the electric and magnetic form factors is determined to be sinΔΦ=-0.67±0.29(stat)±0.18(syst) at sqrt[s]=2.3960 GeV, ΔΦ=55°±19°(stat)±14°(syst) at sqrt[s]=2.6454 GeV, and 78°±22°(stat)±9°(syst) at sqrt[s]=2.9000 GeV. For the first time, the phase of the hyperon electromagnetic form factors is explored in a wide range of four-momentum transfer. The evolution of the phase along with four-momentum transfer is an important input for understanding its asymptotic behavior and the dynamics of baryons.
期刊：
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2024年14:1332666 ISSN：2235-2988
作者机构：
[Ye, Guoguo; Liu, Yingxia; Li, Yanjie; Qing, Ling; Zhang, Li; Yang, Liuqing; Ou, Guanyong; Peng, Ling; Yang, Yang] National Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, The Third People's Hospital of Shenzhen, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen, China;[Ou, Guanyong] School of Medicine, Southern University of Science and Technology, Shenzhen, China;[Qing, Ling; Zhang, Li] Graduate Collaborative Training Base of Shenzhen Third People's Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China
关键词：
hepatitis B vaccine;HGF;IL-5;cytokine;immune response;infant;non/low response
摘要：
BACKGROUND: The immune response to hepatitis B vaccine may be influenced by numerous factors, and patients with non/low response re-exposed to hepatitis B virus remain susceptible. Thus, a better understanding of the underlying mechanisms of non/low immune response in infants born to Hepatitis B surface antigen (HBsAg)-positive mothers is essential. METHODS: 100 infants born to HBsAg-positive mothers from 2015 to 2020 were enrolled in the study, further divided into the non/low response group (n=13) and the moderate strong response group (n=87) based on the quantification of hepatitis B surface antibody at 12 months of age. The differential expression of 48 immune-related cytokines in the two groups was compared and analyzed in detail. The key cytokines were further identified and clinically predictive models were developed. RESULTS: We found that 13 cytokines were lowly expressed and one cytokine was highly expressed in the non/low response group, compared with the moderate strong response group at birth. In addition, 9 cytokines were lowly expressed and one cytokine was highly expressed in the non/low response group at 12 months of age. Furthermore, we found that IL-5 and HGF were promising predictors for predicting the immunization response to hepatitis B vaccine in infants, and the combination of the two cytokines showed the best predictive efficiency, with an area under the curve (AUC) value of 0.844. CONCLUSION: The present study provides a theoretical basis on cytokines for developing and implementing effective immunotherapies against non/low immune response in infants born to HBsAg-positive mothers.
期刊：
JOURNAL OF MATERIALS CHEMISTRY B,2024年12(6):1530-1537 ISSN：2050-750X
通讯作者：
Cheng, D;He, Longwei
作者机构：
[Cheng, Dan; He, Longwei; He, LW; Jiang, Renfeng] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Sch Pharmaceut Sci, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.;[Cheng, Dan; Zhang, Hongshuai; Yang, Xuefeng] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Gastroenterol, Hengyang 421002, Hunan Prov, Peoples R China.;[Cheng, Dan; Liu, Qian; Zhang, Hongshuai; Yang, Xuefeng; Xia, Yuqing] Univ South China, Affiliated Nanhua Hosp, Hunan Prov Clin Res Ctr Metab Associated Fatty Liv, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.
通讯机构：
[Cheng, D ; He, LW] U;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Gastroenterol, Hengyang 421002, Hunan Prov, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Hunan Prov Clin Res Ctr Metab Associated Fatty Liv, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Sch Pharmaceut Sci, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.
摘要：
Carboxylesterases (CESs) are critical for metabolizing ester-containing biomolecules and are specifically important in liver metabolic disorders. The modulation of CESs is also an important issue in pharmacology and clinical applications. Herein, we present a near-infrared (NIR) CES fluorescent probe (NCES) based on the protection-deprotection of the hydroxyl group for monitoring CES levels in living systems. The NCES probe has good selectivity and sensitivity for CESs with a limit of detection (LOD) of 5.24 mU mL-1, which allows for tracing the fluctuation of cellular CES after treatment with anticancer drugs and under inflammation and apoptosis states. Furthermore, NCES can be successfully applied for guiding liver cancer surgery with high-contrast in vivo imaging and detecting clinical serum samples from liver cancer patients. This work showed that the NCES probe has great potential in drug development, imaging applications for medical diagnosis, and early-stage detection for clinical liver diseases. A carboxylesterase-activated near-infrared fluorescent probe with high sensitivity and selectivity was developed to guide surgical resection of liver tumors and monitor clinical serum samples from liver cancer patients.
期刊：
EUROPEAN JOURNAL OF MEDICAL RESEARCH,2024年29(1):1-21 ISSN：0949-2321
通讯作者：
Liu, T;Zhu, GH
作者机构：
[Liu, Tang; Wang, Lifan; Lin, Zhengjun; Wang, Mingrui; Ji, Yuqiao; Yang, Jing; Zhou, Ziting; Yang, Yaocheng] Cent South Univ, Xiangya Hosp 2, Dept Orthoped, Changsha 410011, Hunan, Peoples R China.;[Zhu, Guanghui] Hunan Childrens Hosp, Dept Pediat Orthoped, Hunan Prov Key Lab Pediat Orthoped, Changsha 410007, Hunan, Peoples R China.;[Zhu, Guanghui] Furong Lab, Changsha, Hunan, Peoples R China.;[Zhu, Guanghui] Univ South China, MOE Key Lab Rare Pediat Dis, Hengyang 421001, Hunan, Peoples R China.;[Wang, Mingrui] Cent South Univ, Sch Basic Med Sci, Changsha 410078, Hunan, Peoples R China.
通讯机构：
[Zhu, GH ] H;[Liu, T ] C;Cent South Univ, Xiangya Hosp 2, Dept Orthoped, Changsha 410011, Hunan, Peoples R China.;Hunan Childrens Hosp, Dept Pediat Orthoped, Hunan Prov Key Lab Pediat Orthoped, Changsha 410007, Hunan, Peoples R China.;Furong Lab, Changsha, Hunan, Peoples R China.
摘要：
BACKGROUND: The importance of protein tyrosine phosphatase non-receptor type 3 (PTPN3) in controlling multifaceted tumor cell behaviors throughout cancer development has received widespread attention. Nevertheless, little is known about the biological roles of PTPN3 in drug sensitivity, immunotherapeutic effectiveness, tumor immune microenvironment, and cancer prognosis. METHODS: The Cancer Genome Atlas (TCGA) database's RNAseq data were used to examine the expression of PTPN3 in 33 different cancer types. In addition, immunohistochemistry (IHC) was performed to validate the expression of PTPN3 across various cancer types within our clinical cohorts. The features of PTPN3 alterations were demonstrated throughout the cBioPortal database. This study focused on examining the prognostic and clinicopathological importance of PTPN3 through the acquisition of clinical data from the TCGA database. The investigation of PTPN3's probable role in the tumor immune microenvironment was demonstrated by theapplication of CIBERSORT, ESTIMATE algorithms, and the TISIDB database. Using Spearman's rank correlation coefficient, the relationships between PTPN3 expression and tumor mutation burden (TMB) and microsatellite instability (MSI) were evaluated. To further investigate the putative biological activities and downstream pathways of PTPN3 in various cancers in humans, Gene Set Enrichment Analysis (GSEA) was carried out. In addition, an examination was conducted to explore the associations between PTPN3 and the effectiveness of PD-1/PD-L1 inhibitors,utilizingdata extracted from the GEO database. RESULTS: PTPN3 was abnormally expressed in multiple cancer types and was also strictly associated with the prognosis of cancer patients. IHC was used to investigate and confirm the various expression levels of PTPN3 in various malignancies, including breast cancer, lung cancer, sarcoma, and kidney renal clear cell carcinoma in our clinical cohorts. There is a high correlation between the levels of PTPN3 expression in different cancers and infiltrating immune cells, including mast cells, B cells, regulatory T cells, CD8 + T cells, macrophages, and dendritic cells. Infiltrating immune cells, such as regulatory T cells, CD8 + T cells, macrophages, B cells, dendritic cells, and mast cells, are strongly correlated with PTPN3 expression levels in various tumors. The expression of PTPN3 exhibited a substantial correlation with many immune-related biomolecules and the expression of TMB and MSI in multiple types of cancer. In addition, PTPN3 has demonstrated promise in predicting the therapeutic benefits of PD-1/PD-L1 inhibitors and the susceptibility to anti-cancer medications in the treatment of clinical cancer. CONCLUSIONS: Our findings highlight the importance of PTPN3 as a prognostic biomarker and predictor of immunotherapy success in various forms of cancer. Furthermore, PTPN3 appears to have an important role in modifying the tumor immune microenvironment, highlighting its potential as a promising biomarker for prognosis prediction, immunotherapeutic efficacy evaluation, and identification of immune-related characteristics in diverse cancer types.
作者：
Yang Yang*;Jun Zeng;Boyu Yang;Linmao Yin;Tianyou He
期刊：
International Journal of Refrigeration,2024年160:390-401 ISSN：0140-7007
通讯作者：
Yang Yang
作者机构：
[Tianyou He] School of Civil Engineering, University of South China, Hengyang 421001 Hunan, China;Key Lab of Hunan for the Technologies of Energy Conservation in Prefabricated Buildings, Hengyang 421001, Hunan, China;[Yang Yang; Jun Zeng; Boyu Yang; Linmao Yin] School of Civil Engineering, University of South China, Hengyang 421001 Hunan, China<&wdkj&>Key Lab of Hunan for the Technologies of Energy Conservation in Prefabricated Buildings, Hengyang 421001, Hunan, China
通讯机构：
[Yang Yang] S;School of Civil Engineering, University of South China, Hengyang 421001 Hunan, China<&wdkj&>Key Lab of Hunan for the Technologies of Energy Conservation in Prefabricated Buildings, Hengyang 421001, Hunan, China
摘要：
The mechanical vapor compression system (MVC) coupled with the evaporative-cooling condenser is an effective method to improve its high temperature adaptability. However, this hybrid systems typically use an evaporative cooler as a single-stage pre-cooling unit of condenser and is mainly designed for hot–dry climates. In this paper, based on conventional evaporative-cooling condenser configuration, three two-stage evaporative-cooling condenser systems (MVC-TSEC(A), MVC-TSEC(B) and MVC-TSEC(C)) were proposed. The MVC-TSEC(A) consists of one condenser and an indirect/direct evaporative-cooler. The MVC-TSEC(B) and MVC-TSEC(C) have two condensers (in series), which are coupled with a two-stage evaporative cooling system. The refrigerant in the MVC-TSEC(C) forms a counter-flow configuration with the outdoor air, while it is concurrent flow in MVC-TSEC(B). Subsequently, the effects of the ambient parameters and outdoor air flowrate on the three two-stage hybrid systems were analyzed comparatively based on detailed numerical models. Finally, the application potential of these hybrid systems was evaluated comprehensively in hot–humid climates. The results showed that the seasonal COP and energy-saving rate for the MVC-TSEC(C) are reached by 4.2–4.7 and 12.7–21.1 %, respectively. Moreover, the static equipment payback period of the three two-stage hybrid systems are 3.8, 3.3 and 3.0 years, respectively.
期刊：
Journal of Hazardous Materials,2024年467:133741 ISSN：0304-3894
通讯作者：
Huang, Qunying;Ning, Shunyan
作者机构：
[Huang, Qunying; Zhang, Shichang] Institute of Nuclear Energy Safety Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, PR China;[Zhang, Shichang] University of Science and Technology of China, Hefei 230026, PR China;[Huang, Qunying] University of Science and Technology of China, Hefei 230026, PR China. Electronic address: qunying.huang@inest.cas.cn;[Wei, Yuezhou; Hamza, Mohammed F; Yin, Xiangbiao; Chen, Lifeng] School of Nuclear Science and Technology, University of South China, Hengyang 421001, PR China;[Wu, Kun; Zhong, Yilai; Hu, Fengtao] School of Resources, Environment and Materials, Guangxi University, Nanning 530004, PR China
通讯机构：
[Ning, Shunyan] S;[Huang, Qunying] U;University of Science and Technology of China, Hefei 230026, PR China. Electronic address: qunying.;School of Nuclear Science and Technology, University of South China, Hengyang 421001, PR China. Electronic address:
摘要：
Radioactive strontium ((90)Sr) is considered as one of the most dangerous radionuclides due to its high biochemical toxicity. For the efficient and selective separation of Sr from acidic environments, a novel functional adsorbent CEPA@SBA-15-APTES was prepared in this work through the phosphorylation of amino-modified mesoporous silica with organic content of approximately 20wt%. CEPA@SBA-15-APTES was characterized by TEM, SEM, EDS, TG-DSC, BET, FTIR, and XPS techniques, revealing its characteristics of an ordered hexagonal lattice-like structure and rich functional groups. The experimental results demonstrated that the adsorbent exhibited good adsorption capacity for Sr over a wide acidity range (i.e., from 10(-10) M to 4M HNO(3)). The adsorption equilibriums of Sr by CEPA@SBA-15-APTES in 10(-6) M and 3M HNO(3) solutions were reached within 30 and 5min, respectively, and the adsorption capacities at 318K were 112.6 and 71.8mg/g, respectively. Furthermore, by combining the experimental and characterization results, we found that the adsorption mechanism consisted of ion exchange between Sr(II) and H(+) (in P-OH) in the 10(-6) M HNO(3) solution and coordination between the Sr(II) and oxygen-containing (CO and P=O) functional groups in the 3M HNO(3) solution.
