作者：
Berry, Jesse L.;Pike, Sarah;Rajagopalan, Archeta;Reid, Mark W.;Fabian, Ido didi
期刊：
American Journal of Ophthalmology,2024年260:91-101 ISSN：0002-9394
通讯作者：
Berry, JL
作者机构：
[Pike, Sarah; Rajagopalan, Archeta; Berry, Jesse L.; Reid, Mark W.] Univ Southern Calif, Keck Sch Med, Roski Eye Inst, Los Angeles, CA USA.;[Pike, Sarah; Rajagopalan, Archeta; Berry, Jesse L.; Reid, Mark W.] Univ Southern California, Childrens Hosp Angeles, Keck Sch Med, Los Angeles, CA USA.;[Fabian, Ido didi] Tel Aviv Univ, Goldschleger Eye Inst, Sheba Med Ctr, Tel Hashomer, Israel.;[Fabian, Ido didi] Int Ctr Eye Hlth, London Sch Hyg & Trop Med, London, England.;[Berry, JL; Berry, Jesse L.] Childrens Hosp Los Angeles, Ocular Oncol, 4650 Sunset Blvd, Los Angeles, CA 90027 USA.
通讯机构：
[Berry, JL ] C;Childrens Hosp Los Angeles, Ocular Oncol, 4650 Sunset Blvd, Los Angeles, CA 90027 USA.
摘要：
center dot PURPOSE: Globally, disparities exist in retinoblastoma treatment outcomes between high- and low-income countries, but independent analysis of American countries is lacking. We report outcomes of American retinoblastoma patients and explore factors associated with survival and globe salvage. center dot DESIGN: Subanalysis of prospective cohort study data. center dot METHODS: Multicenter analysis at 57 American treatment centers in 23 countries of varying economic levels (low income [LIC], lower-middle income [LMIC], upper-middle income [UMIC], and high income [HIC]) of 491 treatment-naive retinoblastoma patients diagnosed in 2017 and followed through 2020. Survival and globe salvage rates analyzed with Kaplan-Meier analysis and Cox proportional hazard models. center dot RESULTS: Of patients, 8 (1.6%), 58 (11.8%), 235 (47.9%), and 190 (38.7%) were from LIC, LMIC, UMIC, and HIC groups, respectively. Three-year survival rates in LICs were 60.0% (95% confidence interval [CI] 12.6-88.2) compared with 99.2% (95% CI 94.6%-99.9%) in HICs. Death was less likely in patients > 4 years of age (vs <= 4 years, HR = 0.45 [95% CI 0.27-0.78], P = .048). Patients with more advanced tumors (eg, cT3 vs cT1, HR = 4.65 x 109 [95% CI 1.25 x 109 -1.72 x 10(10)], P < .001) and females (vs males, HR = 1.98 [95% CI 1.27-3.10], P = .04) were more likely to die. Three-year globe salvage rates were 13.3% (95% CI 5.1%-25.6%) in LMICs and 46.2% (95% CI 38.8%-53.3%) in HICs. At 3 years, 70.1% of cT1 eyes (95% CI 54.5%-81.2%) vs 8.9% of cT3 eyes (95% CI 5.5%-13.3%) were salvaged. Advanced tumor stage was associated with higher enucleation risk (eg, cT3 vs cT1, subhazard ratio = 4.98 [95% CI 2.36-10.5], P < .001). center dot CONCLUSIONS: Disparities exist in survival and globe salvage in American countries based on economic level and tumor stage demonstrating a need for childhood cancer programs. (Am J Ophthalmol 2024;260: 91-101. (c) 2023 Elsevier Inc. All rights reserved.)
期刊：
Physica Status Solidi-Rapid Research Letters,2024年18(2):2300334- ISSN：1862-6254
通讯作者：
Huo, SY
作者机构：
[Fu, Chun-Ming; Li, Hong-Kang; Huo, Shao-Yong; Yao, Long-Chao] Univ South China, Coll Mech Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Huo, SY ] U;Univ South China, Coll Mech Engn, Hengyang 421001, Peoples R China.
关键词：
defect states;elastic valley edge states;power dividers;rainbow trapping;topological phononic crystals
摘要：
Topological phononic crystals (PnCs) with topologically protected boundary states have important applications in the fields of acoustic wave transmission and control. However, previous studies based on solid-state PnC systems are mostly limited by fixed structures, resulting in the difficulty to deform the edge states, which partly limits its practical applications. Herein, a 2D solid topological PnC coupled with the defect is designed to achieve the adjustable valley edge state and rainbow trapping. First, by breaking the spatial inversion symmetry, the valley Hall phase transition of elastic wave is realized and valley edge states are obtained. Next, by introducing defects of different widths between the two different valleys' topological PnCs, both the defect-adjusted valley edge state and defect state are achieved. Then, by designing different topological PnCs waveguides, the robust transport characteristics of the two above states are compared. Subsequently, a new power divider based on the defect-adjusted valley edge state is designed, which is found to possess various manners of operation such as equal and unequal power divisions. Finally, based on defect adjustment of the edge states, a rainbow trapping is implemented. This research provides an important guidance for ultrasonic devices, such as waveguides, energy harvesters, and power dividers. By introducing the line defects in the topological domain wall for the elastic wave, the defect-adjusted valley edge states and defect states are presented, which further are applied to design the novel elastic ultrasonic devices, such as the equal or unequal power dividers and the rainbow trapping effects.image & COPY; 2023 WILEY-VCH GmbH
摘要：
Electrosorption holds extraordinary promise for uranium (U(VI)) capture but is still limited by the finite accessible active sites of electrode materials, poor surface wettability and its intrinsic co-ion repulsion effect. Here we originally designed KOH-etched porous graphitic carbon nitride (g-C3N4) as active material, and multi-functional crosslinked xanthan gum-polyacrylic acid (XG-PAA) as binder to fabricate g-C3N4-X electrode for U(VI) capture. The abundant in-plane nanopores on 2D g-C3N4 lamellar, rich O- and N-containing active sites, and 3D tough interconnected conductive architecture, endowed g-C3N4-X electrode with fast electron/ion transport channels, alleviated co-ions exclusion, superior electrochemical properties, and hydrophilicity. These unique structure characteristics enabled U(VI) ions to form micro electric-field attraction with negatively charged COO- groups in advance to alleviate co-ion repulsion, then to electro-migrate towards and coordinate with active sites, and eventually to be electrocatalytic reduced into U(IV) deposits. Consequently, the g-C3N4-X electrode exhibited a 1.87 times faster adsorption kinetics, a much higher removal ratio of 98.1% within 70 min than traditional PVDF-bound electrode. Meanwhile, the cumulative adsorption capacity of g-C3N4-X electrode after six cycles was up to 1459.1 mg g−1. This efficient, scalable, and cost-effective g-C3N4-X electrode represents a significant step forward toward the practical applications of U(VI) electrosorption.
摘要：
Asparagine, an important amino acid in mammals, is produced in several organs and is widely used for the production of other nutrients such as glucose, proteins, lipids, and nucleotides. Asparagine has also been reported to play a vital role in the development of cancer cells. Although several types of cancer cells can synthesise asparagine alone, their synthesis levels are insufficient to meet their requirements. These cells must rely on the supply of exogenous asparagine, which is why asparagine is considered a semi-essential amino acid. Therefore, nutritional inhibition by targeting asparagine is often considered as an anti-cancer strategy and has shown success in the treatment of leukaemia. However, asparagine limitation alone does not achieve an ideal therapeutic effect because of stress responses that upregulate asparagine synthase (ASNS) to meet the requirements for asparagine in cancer cells. Various cancer cells initiate different reprogramming processes in response to the deficiency of asparagine. Therefore, it is necessary to comprehensively understand the asparagine metabolism in cancers. This review primarily discusses the physiological role of asparagine and the current progress in the field of cancer research.
摘要：
Humans tend to mine objects by learning from a group of images or several frames of video since we live in a dynamic world. In the computer vision area, many researchers focus on co-segmentation (CoS), co-saliency detection (CoSD) and video salient object detection (VSOD) to discover the co-occurrent objects. However, previous approaches design different networks for these similar tasks separately, and they are difficult to apply to each other. Besides, they fail to take full advantage of the cues among inter- and intra-feature within a group of images. In this paper, we introduce a unified framework to tackle these issues from a unified view, term as <bold>UFGS</bold> (<bold>U</bold>nified <bold>F</bold>ramework for <bold>G</bold>roup-based <bold>S</bold>egmentation). Specifically, we first introduce a transformer block, which views the image feature as a patch token and then captures their long-range dependencies through the self-attention mechanism. This can help the network to excavate the patch-structured similarities among the relevant objects. Furthermore, we propose an intra-MLP learning module to produce self-mask to enhance the network to avoid partial activation. Extensive experiments on four CoS benchmarks (PASCAL, iCoseg Internet and MSRC), three CoSD benchmarks (Cosal2015, CoSOD3k, and CocA) and five VSOD benchmarks (DAVIS(16), FBMS, ViSal, SegV2, and DAVSOD) show that our method outperforms other state-of-the-arts on three different tasks in both accuracy and speed by using the same network architecture, which can reach 140 FPS in real-time.
作者机构：
[Dai, Hailu; He, Shoucheng] Yancheng Inst Technol, Sch Mat Sci & Engn, Yancheng 224051, Peoples R China.;[Bi, Lei] Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Bi, L ] U;Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.
关键词：
Sb doping;Cathode;LaFeO3;PCFCs;SOFCs
摘要：
The La0.5Sr0.5FeO3-delta (LSF) material is modified with Sb doping to improve the performance of conventional LSF cathode for protonic ceramic fuel cells (PCFCs). The Sb cations can be inserted into the LSF lattice to develop a new composition La0.5Sr0.5Fe0.9Sb0.1O3-delta (LSFSb), which reduces the formation energy of oxygen vacancies and lowers the proton migration energy of the Sb-doped material when compared to the Sb-free LSF. Additional experimental findings reveal that Sb doping enhances the proton/oxygen transport and surface exchange kinetics. The fuel cell with the LSFSb cathode has a substantially higher peak power density of 933 mW cm-2 at 700 degrees C than the LSF cell, which only reaches 606 mW cm-2 under the same testing conditions. Using the LSFSb composite cathode further improved the fuel-cell performance, resulting in a promising fuel cell performance of 1388 mW cm-2, which is high for LSF-based PCFCs, thus demonstrating that LSFSb is a suitable cathode for PCFCs.
摘要：
Recent advances in melanoma therapy have significantly improved the prognosis of metastasized melanoma. However, large therapeutic gaps remain that need to be closed by new strategies. Antiapoptotic Bcl-2 proteins critically contribute to apoptosis deficiency and therapy resistance. They can be targeted by BH3 mimetics, small molecule antagonists that mimic the Bcl-2 homology domain 3 (BH3) of proapoptotic BH3-only proteins. By applying in vitro experiments, we aimed to obtain an overview of the possible suitability of BH3 mimetics for future melanoma therapy. Thus, we investigated the effects of ABT-737 and ABT-263, which target Bcl-2, Bcl-x(L) and Bcl-w as well as the Bcl-2-selective ABT-199 and the Mcl-1-selective S63845, in a panel of four BRAF-mutated and BRAF-WT melanoma cell lines. None of the inhibitors showed significant effectiveness when used alone; however, combination of S63845 with each one of the three ABTs almost completely abolished melanoma cell survival and induced apoptosis in up to 50-90% of the cells. Special emphasis was placed here on the understanding of the downstream pathways involved, which may allow improved applications of these strategies. Thus, cell death induction was correlated with caspase activation, loss of mitochondrial membrane potential, phosphorylation of histone H2AX, and ROS production. Caspase dependency was demonstrated by a caspase inhibitor, which blocked all effects. Upregulation of Mcl-1, induced by S63845 itself, as reported previously, was blocked by the combinations. Indeed, Mcl-1, as well as XIAP (X-linked inhibitor of apoptosis), were strongly downregulated by combination treatments. These findings demonstrate that melanoma cells can be efficiently targeted by BH3 mimetics, but the right combinations have to be selected. The observed pronounced activation of apoptosis pathways demonstrates the decisive role of apoptosis in the loss of cell viability by BH3 mimetics.
期刊：
IEEE Transactions on Information Forensics and Security,2024年19:3093-3108 ISSN：1556-6013
通讯作者：
Li, Q
作者机构：
[Jiang, Fangling] Univ South China, Sch Comp Sci, Hengyang 421001, Hunan, Peoples R China.;[Liu, Yunfan] Univ Chinese Acad Sci, Sch Elect Elect & Commun Engn, Beijing 101408, Peoples R China.;[Meng, Jingjing; Si, Haolin] Huawei Technol Co Ltd, Shenzhen 518129, Guangdong, Peoples R China.;[Li, Qi; Li, Q] CASIA, Ctr Res Intelligent Percept & Comp, State Key Lab Multimodal Artificial Intelligence S, Beijing 100190, Peoples R China.;[Li, Qi] Univ Chinese Acad Sci, Sch Artificial Intelligence, Beijing 100049, Peoples R China.
通讯机构：
[Li, Q ] C;CASIA, Ctr Res Intelligent Percept & Comp, State Key Lab Multimodal Artificial Intelligence S, Beijing 100190, Peoples R China.
关键词：
Face anti-spoofing;cross-scenario testing;generalized feature learning;unknown PAI detection
摘要：
In recent years, domain adaptation techniques have been widely used to adapt face anti-spoofing models to a cross-scenario target domain. Most previous methods assume that the Presentation Attack Instruments (PAIs) in such cross-scenario target domains are the same as in the source domain. However, since malicious users are free to use any form of unknown PAIs to attack the system, this assumption does not always hold in practical applications of face anti-spoofing. Thus, unknown PAIs would inevitably lead to significant performance degradation, since samples of known and unknown PAIs usually have large differences. In this paper, we propose an Evidential Semantic Consistency Learning (ESCL) framework to address this problem. Specifically, a regularized evidential deep learning strategy with a two-way balance of class probability and uncertainty is leveraged to produce uncertainty scores for unknown PAI detection. Meanwhile, an entropy optimization-based semantic consistency learning strategy is also employed to encourage features of live and known PAIs to be gathered in the label-conditioned clusters across the source and target domains, while making the features of unknown PAIs to be self-clustered according to intrinsic semantic information. In addition, a new evaluation metric, KUHAR, is proposed to comprehensively evaluate the error rate of known classes and unknown PAIs. Extensive experimental results on six public datasets demonstrate the effectiveness of our method in generalizing face anti-spoofing models to both known classes and unknown PAIs with different types and quantities in a cross-scenario testing domain. Our method achieves state-of-the-art performance on eight different protocols.
作者机构：
[Zhang, Jinjie; Mao, Zhiwei; Xiong, Guoqing; Zhang, JJ] Beijing Univ Chem Technol, Key Lab Engine Hlth Monitoring Control & Networkin, Minist Educ, Beijing 100029, Peoples R China.;[Xiang, Liping; Jiang, Qinglei; Bao, Binbin; Mao, Zhiwei] Univ South China, Key Lab Adv Nucl Energy Technol Design & Safety, Minist Educ, Hengyang 421099, Peoples R China.;[Jiang, Qinglei; Bao, Binbin] China Nucl Power Operat Technol Co Ltd, Wuhan 430223, Peoples R China.
通讯机构：
[Mao, ZW ; Zhang, JJ] B;Beijing Univ Chem Technol, Key Lab Engine Hlth Monitoring Control & Networkin, Minist Educ, Beijing 100029, Peoples R China.;Univ South China, Key Lab Adv Nucl Energy Technol Design & Safety, Minist Educ, Hengyang 421099, Peoples R China.
摘要：
The interaction of temperature variations and viscoelastic deformation is critical in dynamically loaded journal bearings, especially under fluctuating heavy load conditions. Previous studies have not fully explored the coupling of these two factors. Addressing this gap, this research introduces a novel transient mixed lubrication model that simultaneously considers thermal and viscoelastic effects. This model reveals intricate dynamics influencing the lubrication performance of journal bearings. The findings show that the combined impact of thermal and viscoelastic factors significantly alters the amplitude and phase of lubrication performance parameters. Notably, thermal effects tend to decrease oil film pressure while increasing asperity contact pressure, whereas viscoelastic effects enhance the oil film pressure peak and adjust its phase, concurrently reducing asperity contact pressure. Moreover, varying the eccentric speed profoundly influences the interplay between bearing thermal and viscoelastic effects. This study contributes to a deeper understanding of mixed lubrication in journal bearings and provides technical support for more accurate and predictive reliability improvement in complex mechanical systems.
期刊：
Scandinavian Journal of Immunology,2024年99(3):e13357- ISSN：0300-9475
通讯作者：
Lei, AH;Zhang, HJ
作者机构：
[Long, WeiXiang; Lei, AH; Lei, Aihua; Dai, Zhongling; Liu, Duo; Gong, Zhande; Wang, Cui] Univ South China, Inst Pathogen Biol, Sch Basic Med Sci, Hengyang Med Sch, 28 Changsheng Western Rd, Hengyang 421001, Hunan, Peoples R China.;[Long, WeiXiang; Lei, Aihua; Dai, Zhongling; Liu, Duo; Gong, Zhande; Wang, Cui] Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Con, Hengyang, Peoples R China.;[Long, WeiXiang; Lei, Aihua; Dai, Zhongling; Liu, Duo; Gong, Zhande; Wang, Cui] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang, Peoples R China.;[Zhang, Haijun] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Cardiol, 69 Chuanshan Ave, Hengyang 421000, Hunan, Peoples R China.
通讯机构：
[Lei, AH ; Zhang, HJ ] U;Univ South China, Inst Pathogen Biol, Sch Basic Med Sci, Hengyang Med Sch, 28 Changsheng Western Rd, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Cardiol, 69 Chuanshan Ave, Hengyang 421000, Hunan, Peoples R China.
关键词：
allergic airway inflammation;hormones;ILC2s
摘要：
Group 2 innate lymphoid cells (ILC2s) are a type of innate immune cells that produce a large amount of IL-5 and IL-13 and two cytokines that are crucial for various processes such as allergic airway inflammation, tissue repair and tissue homeostasis. It is known that damaged epithelial-derived alarmins, such as IL-33, IL-25 and thymic stromal lymphopoietin (TSLP), are the predominant ILC2 activators that mediate the production of type 2 cytokines. In recent years, abundant studies have found that many factors can regulate ILC2 development and function. Hormones synthesized by the body's endocrine glands or cells play an important role in immune response. Notably, ILC2s express hormone receptors and their proliferation and function can be modulated by multiple hormones during allergic airway inflammation. Here, we summarize the effects of multiple hormones on ILC2-driven allergic airway inflammation and discuss the underlying mechanisms and potential therapeutic significance.
期刊：
Journal of Biological Chemistry,2024年300(3):105721 ISSN：0021-9258
通讯作者：
Wang, Yugang
作者机构：
[Zeng, Xiao; Zhou, Runxin; Wang, Yu; Tong, Fuqiang; Guo, Dingyuan; Yin, Sibi; Wang, Yugang] Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China;[Yu, Weixing] Department of Biochemistry and Molecular Biology, College of Basic Medicine, Jining Medical University, Jining, 272067, China;[Jiang, Li] Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, China, 430060;[Jiang, Li] Department of Neurology, The Affiliated Nanhua Hospital, University of South China, Hengyang, 421001, China;[He, Leya] Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China
通讯机构：
[Yugang Wang] D;Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China<&wdkj&>Cell Architecture Research Center, Huazhong University of Science and Technology, Wuhan, Hubei, China
关键词：
histone sulfation;regulation of gene transcription;hypoxia;glycolysis;PDK1
摘要：
Histone H3 tyrosine-99 sulfation (H3Y99sulf) is a recently identified histone mark that can cross-talk with H4R3me2a to regulate gene transcription, but its role in cancer biology is less studied. Here, we report that H3Y99sulf is a cancer-associated histone mark that can mediate hepatocellular carcinoma (HCC) cells responding to hypoxia. Hypoxia-stimulated SNAIL pathway elevates the expression of PAPSS2, which serves as a source of adenosine 3′-phosphate 5′-phos-phosulfate for histone sulfation and results in upregulation of H3Y99sulf. The transcription factor TDRD3 is the downstream effector of H3Y99sulf-H4R3me2a axis in HCC. It reads and co-localizes with the H3Y99sulf-H4R3me2a dual mark in the promoter regions of HIF1A and PDK1 to regulate gene transcription. Depletion of SULT1B1 can effectively reduce the occurrence of H3Y99sulf-H4R3me2a-TDRD3 axis in gene promoter regions and lead to downregulation of targeted gene transcription. Hypoxia-inducible factor 1-alpha and PDK1 are master regulators for hypoxic responses and cancer metabolism. Disruption of the H3Y99sulf-H4R3me2a-TDRD3 axis can inhibit the expression and functions of hypoxia-inducible factor 1-alpha and PDK1, resulting in suppressed proliferation, tumor growth, and survival of HCC cells suffering hypoxia stress. The present study extends the regulatory and functional mechanisms of H3Y99sulf and improves our understanding of its role in cancer biology.
作者机构：
[Chen, Alex F.; Zhang, Zhen; Yu, Fan] Cent South Univ, Dept Cardiol, Xiangya Hosp 3, Changsha, Peoples R China.;[Yu, Fan] Zhejiang Univ, Res Ctr Life Sci & Human Hlth, Binjiang Inst, Hangzhou, Zhejiang, Peoples R China.;[Leng, Yiping] Univ South China, Affiliated Changsha Cent Hosp, Res Ctr PhaseClin Trials 1, Hengyang Med Sch, Changsha, Hunan, Peoples R China.;[Chen, Alex F.] Shanghai Jiao Tong Univ, Inst Cardiovasc Dev & Regenerat Med, Dept Cardiol, Sch Med,Xinhua Hosp, 1665 Kongjiang Rd, Shanghai 200092, Peoples R China.
通讯机构：
[Chen, AF ] C;Cent South Univ, Dept Cardiol, Xiangya Hosp 3, Changsha, Peoples R China.;Shanghai Jiao Tong Univ, Inst Cardiovasc Dev & Regenerat Med, Dept Cardiol, Sch Med,Xinhua Hosp, 1665 Kongjiang Rd, Shanghai 200092, Peoples R China.
关键词：
GSDMD;O-GlcNAc;Sepsis;Endothelial;Pyroptosis
摘要：
OBJECTIVE: Increased O-linked β-N-acetylglucosamine (O-GlcNAc) stimulation has been reported to protect against sepsis associated mortality and cardiovascular derangement. Previous studies, including our own research, have indicated that gasdermin-D(GSDMD)-mediated endothelial cells pyroptosis contributes to sepsis-associated endothelial injury. This study explored the functions and mechanisms of O-GlcNAc modification on lipopolysaccharide (LPS)-induced pyroptosis and its effects on the function of GSDMD. METHODS: A LPS-induced septic mouse model administrated with O-GlcNAcase (OGA) inhibitor thiamet-G (TMG) was used to assess the effects of O-GlcNAcylation on sepsis-associated vascular dysfunction and pyroptosis. We conducted experiments on human umbilical vein endothelial cells (HUVECs) by challenging them with LPS and TMG to investigate the impact of O-GlcNAcylation on endothelial cell pyroptosis and implications of GSDMD. Additionally, we identified potential O-GlcNAcylation sites in GSDMD by utilizing four public O-GlcNAcylation site prediction database, and these sites were ultimately established through gene mutation. RESULTS: Septic mice with increased O-GlcNAc stimulation exhibited reduced endothelial injury, GSDMD cleavage (a marker of pyroptosis). O-GlcNAc modification of GSDMD mitigates LPS-induced pyroptosis in endothelial cells by preventing its interaction with caspase-11 (a human homologous of caspases-4/5). We also identified GSDMD Serine 338 (S338) as a novel site of O-GlcNAc modification, leading to decreased association with caspases-4 in HEK293T cells. CONCLUSIONS: Our findings identified a novel post-translational modification of GSDMD and elucidated the O-GlcNAcylation of GSDMD inhibits LPS-induced endothelial injury, suggesting that O-GlcNAc modification-based treatments could serve as potential interventions for sepsis-associated vascular endothelial injury.
摘要：
This paper presents a theoretical solution for bond-slip behavior of SRC interface based on experiment, numerical simulation, and theoretical derivation. Push-out tests of five specimens were firstly carried out, based on which, the simplified bond-slip model was proposed. The specimen was fabricated using the Q345H-shape steel (400 mm x 200 mmx13 mm x 8 mm) and C50 concrete (350 mm x 600 mm) with a bonding length of 500 mm. A FE model was established based on the bond-slip model to analyze the nonlinear bonding stress that are difficult to obtain from the experiment. The research shows that although the bonding stress in the elastic stage is not uniformly distributed, it is basically uniformly distributed when the ultimate bearing capacity is reached. It proves that assuming the average interfacial bonding stress under ultimate load to replace the maximum bonding stress is reasonable, which is crucial for designer to estimate interface bearing capacity and maximum bonding stress. Moreover, five different stages were obtained by the FE model analysis and the theoretical equations of bonding stress nonlinear distribution were obtained based on the boundary condition of each stage. The theoretical solution clearly showed the relationship between interface nonlinear stress distribution and influencing parameters including the bond area, elasticity modulus of material, section area of specimen, bond-slip constitutive model, and the external load. Based on the theoretical model, the influences of different parameters can be directly obtained without conducting further experiments and establishing different FE models.
摘要：
Van der Hoeve's syndrome, also known as osteogenesis imperfecta (OI), is a genetic connective tissue disorder characterized by fragile, fracture-prone bone and hearing loss. The disease is caused by a gene mutation in one of the two type I collagen genes COL1A1 or COL1A2. In this study, we identified a novel frameshift mutation of the COL1A1 gene (c.1607delG) in a family with OI using whole-exome sequencing, bioinformatics analysis and Sanger sequencing. This mutation may lead to the deletion of a portion of exon 23 and the generation of a premature stop codon in the COL1A1 gene. To further investigate the impact of this mutation, we established two induced pluripotent stem cell (iPSC) lines from peripheral blood mononuclear cells of OI patients carrying a novel mutation in the COL1A1 gene. Osteoblasts (OB) derived from OI-iPSCs exhibited reduced production of type I collagen and diminished ability to differentiate into osteoblasts. Using a CRISPR-based homology-directed repair strategy, we corrected the OI disease-causing COL1A1 novel mutations in iPSCs generated from an affected individual. Our results demonstrated that the diminished expression of type I collagen and osteogenic potential were enhanced in OB induced from corrected OI-iPSCs compared to those from OI-iPSCs. Overall, our results provide new insights into the genetic basis of Van der Hoeve's syndrome and highlight the potential of iPSC technology for disease modeling and therapeutic development.
摘要：
Nanozymes have become promising alternatives to antibiotics for fighting bacteria owing to their broadspectrum antibacterial activity, robust stability, and multifunctionality. However, the deficient activity of nanozymes and the overexpressed glutathione in the infectious microenvironments extensively hamper their antibacterial efficiency. Herein, we present a norepinephrine (NE) induced Pd metallic aerogel as a highly efficient hydrophilic nanozyme for photothermal-enhanced chemodynamic therapy for bacterial infections and wound healing. The prepared NE-Pd aerogels exhibit satisfactory peroxidase-like and oxidase-like activity, which could generate an amount of hydroxyl radical and superoxide anion radical to cause irreversible damage to bacteria and decompose biofilm components with no bacteria resistance. In addition, the glutathione peroxidaselike activity of NE-Pd aerogels could deplete glutathione to adjust the infectious microenvironments for intensifying the generation of ROS, thus boosting the chemodynamic therapeutic efficacy. Furthermore, the intrinsic photothermal effect of NE-Pd aerogels generates hyperthermia to impair bacteria and further substantially promotes the multienzyme-like activity to damage bacteria and their biofilms. Consequently, the animal skinwound model results further denoted that NE-Pd aerogels could effectively cure methicillin-resistant Staphylococcus aureus (MRSA) infected wounds with excellent in vivo biocompatibility in either wound tissue or living organisms. This work demonstrates that NE-Pd aerogel nanozymes have tremendous potential as a synergistically photothermal-catalytic antibacterial agent for treating bacterial infection and accelerating wound healing.
作者机构：
[Wang, Yang; Fu, Danning] State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510641, China;[Yang, Rendang] State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510641, China. Electronic address: rendangyang@163.com;[Wang, Ruibin] College of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China. Electronic address: rbwang@usc.edu.cn;[Li, Yan] Longyou Inspection and Testing Institute, Quzhou 324000, China;[Bian, Huiyang] International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China
通讯机构：
[Wang, Ruibin] C;[Yang, Rendang] S;State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510641, China. Electronic address:;College of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China. Electronic address:
摘要：
Flexible conductive hydrogels (FCHs) have attracted widespread interest as versatile monoliths that can be intricately integrated with various ingredients boasting multiple functionalities. The chemicophysical properties of FCHs cover a wide range, which significantly vary in their building blocks. However, achieving both favorable mechanical strength and high conductivity simultaneously through a facile approach remains a challenge. Herein, polyvinyl alcohol, dialdehyde cellulose nanofibrils, silver nanoparticles, borax, and tannic acid are readily "one-pot" incorporated into FCHs with great tensile stress (499kPa), tensile strain (4591%), and compressive stress (269kPa) due to abundant hydrogen bonding, dynamic borate-diol bonding, and intermolecular acetalization. They also exhibit desired self-healing, generalized-adhesive, and antibacterial performances. Taking advantage of these, FCHs are further employed to support an epidermal sensor, on which remarkable strain sensitivity (gauge factor=8.22), high-pressure sensitivity (≥ 0.258kPa(-1)), and fast response (≤ 190ms) are recorded. Its highly adaptive mechano-electric transformability and functions can be well maintained in serving as an array unit and touch screen pen. The results well addressed in this work are anticipated to pave the universal way of engineering FCHs.
摘要：
Diabetic nephropathy (DN) is one of the most serious and general complications of diabetes, while schedules for prevention and treatment are unsatisfactory. Leucine aminopeptidase (LAP) as one of the biomarkers of DN is associated with glomerular and/or tubular injury. Imaging of LAP activity in DN disease model in vivo is thus beneficial for early diagnosis and prevention of DN, but such a strategy is still lacking. Herein, an enzyme-activated probe HD-LAP with a NIR fluorescence emission for specific detection of LAP activity in the DN model is designed and synthesized. HD-LAP has a significant fluorescence enhancement after reacted with LAP and shows a NIR fluorescence emission at 704 nm based on intramolecular charge transfer mechanism. Moreover, HD-LAP can be employed to image LAP activity in HK-2 and HepG2 cells. More importantly, HD-LAP is the first example to real-time image LAP in DN mice and clinical serum samples. These results demonstrated that HD-LAP is promising as a powerful tool for the research on LAP associated diabetic diseases in future.
通讯机构：
[Xu, BM ; Wang, XZ] S;[Wang, XZ ] U;Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China.;Univ South China, Sch Elect Engn, Hengyang 421001, Peoples R China.;Southern Univ Sci & Technol, Shenzhen Engn Res & Dev Ctr Flexible Solar Cells, Shenzhen 518055, Peoples R China.
摘要：
As the most promising lead-free perovskite, tin-based perovskite has attracted much attention due to its suitable bandgap and low toxicity. However, many defects (tin vacancy defects caused by Sn2+ oxidation to Sn4+, grain boundary defects and point defects) in tin-based perovskite lead to serious losses in device efficiency. Here, a universal and effective doping strategy is delivered to improve the structure and optoelectronic properties of tin-based perovskite films through 2,8-dibromo-dibenzothiophene-S,S-dioxide (BrDS) doping. The introduction of BrDS can effectively inhibit the oxidation of Sn2+ and passivate grain boundary defects and point defects in the perovskite films. The tin-based perovskite film doped with BrDS exhibits higher carrier lifetime and crystal quality. As a result, the BrDS-doped device achieves a power conversion efficiency of 14.98% with a certified efficiency of 14.36%, which is one of the highest PCEs among all values reported to date for tin-based PSCs. In addition, the BrDS-doped PSC devices exhibit significantly improved long-term stability and improved operational stability in a N-2 atmosphere. This work represents a noteworthy step towards manufacturing efficient and stable tin-based PSCs.
