期刊：
Molecular and Cellular Biochemistry,2024年479(4):779-791 ISSN：0300-8177
通讯作者：
Zhisheng Jiang
作者机构：
[He, Shuya; He, Siqi; Jiang, Zhisheng; Ma, Yun; Gu, Tianhe] Univ South China, Inst Biochem & Mol Biol, Hengyang Med Coll, Hengyang 421001, Hunan, Peoples R China.;[Jiang, Zhisheng; Ma, Yun] Univ South China, Inst Cardiovasc Dis, Hengyang Med Sch, Key Lab Arteriosclerol Hunan Prov,Hunan Int Sci &, Hengyang 421001, Hunan, Peoples R China.;[Ma, Yun] Univ South China, Hengyang Med Sch, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zhisheng Jiang] I;Institute of Biochemistry and Molecular Biology, Hengyang Medical College, University of South China, Hengyang, China<&wdkj&>Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan Province, Hengyang Medical School, Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic Disease, University of South China, Hengyang, China
摘要：
Cardiovascular disease (CVD) has a high incidence and low cure rate worldwide, and atherosclerosis (AS) is the main factor inducing cardiovascular disease, of which lipid deposition in the vessel wall is the main marker of AS. Currently, although statins can be used to lower lipids and low-density lipoprotein (LDL) in AS, the cure rate for AS remains low. Therefore, there is an urgent need to develop new therapeutic approaches, and stem cells are now widely studied, while stem cells are a class of cell types that always maintain the ability to differentiate and can differentiate to form other cells and tissues, and stem cell transplantation techniques have shown efficacy in the treatment of other diseases. With the establishment of cellular therapies and continued research in stem cell technology, stem cells are also being used to address the problem of AS. In this paper, we focus on recent research advances in stem cell therapy for AS and briefly summarize the relevant factors that induce the formation of AS. We mainly discuss the efficacy and application prospects of mesenchymal stem cells (MSCs) for the treatment of AS, in addition to the partial role and potential of exosomes in the treatment of AS. Further, provide new ideas for the clinical application of stem cells.
摘要：
Long non-coding RNA (lncRNA) is a kind of biomolecule that can regulate important life activities such as cell proliferation, apoptosis, differentiation, aging, and body development. It has been found that lncRNAs are closely related to various diseases. In cardiovascular diseases, lncRNAs affect the expression level of related genes in atherosclerotic plaques, which are closely related to endothelial dysfunction, smooth muscle cell proliferation, macrophage dysfunction, abnormal lipid metabolism, and cellular autophagy, thus participating in regulating the occurrence and development of AS. In view of this, investigating the role of lncRNAs in regulating cardiac gene networks on cardiovascular system diseases has attracted much clinical attention and may be a novel target for AS therapy. This paper focuses on lncRNAs related to AS, explores the relationship between lncRNAs and AS, suggests the role of lncRNAs in the prevention and treatment of AS, and expects the application of more lncRNAs as the marker in the clinical diagnosis and treatment of AS.
作者机构：
[Wang, Wuzhou; He, Shuya; Shen, Wangtao; Qi, Huizhou; Ma, Yun] Univ South China, Inst Biochem & Mol Biol, Hengyang Med Coll, Hengyang 421001, Peoples R China.;[Qi, Huizhou] Univ South China, Hengyang Med Coll, Funct Lab Ctr, Hengyang 421001, Peoples R China.;[Wang, Wuzhou; He, Shuya; Qi, Huizhou; Xiao, Fangzhu] Univ South China, Hengyang Key Lab Biol Effects Nucl Radiat, Hengyang 421001, Peoples R China.;[Zhu, Hui] Univ South China, Inst Engn Math, Math & Phys Coll, Hengyang 421001, Peoples R China.;[He, Junyan] Univ South China, Affiliated Hosp 1, Dept Radiat Oncol, Hengyang 421001, Peoples R China.
通讯机构：
[Ma, Yun] I;[He, Shuya] H;Institute of Biochemistry and Molecular Biology, Hengyang Medical College, University of South China, Hengyang, 421001, China. Electronic address:;Hengyang Key Laboratory for Biological Effects of Nuclear Radiation, University of South China, Hengyang, 421001, China. Electronic address:
关键词：
DNA double-strand break;DSB reaction intermediate;Kinetics of DSB repair;Mathematical model
摘要：
This manuscript outlines the kinetics of two main repair pathways of DNA double-strand break (DSB) in eukaryotes: non-homologous end joining (NHEJ) and homologous recombination repair (HRR). In this review, we discuss the precise study of recruitment kinetics of repair proteins based on the latest technologies in the past two decades. Then we simulate the theoretical description of the DNA repair process by mathematical models. In our study, the consecutive reactions chain (CRC) model and continuous-time random walk (CTRW) model have been unified by us, so that we can obtain the function of the number of intermediates with time in the same framework of equations, overcome the incompatibility between the two models. On this basis, we propose a data fitting workflow using these both models. Finally, we give an overview of different real-time quantitative methods and the new mechanism complexity that can be found from the corresponding dynamic models.
期刊：
Research in Microbiology,2020年171(2):45-54 ISSN：0923-2508
通讯作者：
He, Shu-ya
作者机构：
[He, Shu-ya; Wang, Wu-zhou; He, Jun-yan; Ma, Yun; Qi, Hui-zhou] Univ South China, Hengyang Med Coll, Inst Biochem & Mol Biol, Hengyang 421001, Peoples R China.;[Qi, Hui-zhou] Univ South China, Hengyang Med Coll, Funct Lab Ctr, Hengyang 421001, Peoples R China.;[He, Shu-ya; Wang, Wu-zhou; He, Jun-yan; Xiao, Fang-zhu; Qi, Hui-zhou] Univ South China, Hengyang Key Lab Biol Effects Nucl Radiat, Hengyang 421001, Peoples R China.
通讯机构：
[He, Shu-ya] U;Univ South China, Hengyang Key Lab Biol Effects Nucl Radiat, Hengyang 421001, Peoples R China.
关键词：
Antioxidative system;Deinococcus radiodurans;Manganese;PprI;PprM;Reactive oxygen species
摘要：
Deinococcus radiodurans is famous for its extreme resistance to various stresses such as ionizing radiation (IR), desiccation and oxidative stress. The underlying mechanism of exceptional resistance of this robust bacterium still remained unclear. However, the antioxidative system of D. radiodurans has been considered to be the determinant factor for its unparalleled resistance and protects the proteome during stress, then the DNA repair system and metabolic system exert their functions to restore the cell to normal physiological state. The antioxidative system not only equipped with the common reactive oxygen species (ROS) scavenging enzymes (e.g. catalase and superoxide dismutase) but also armed with a variety of non-enzyme antioxidants (e.g. carotenoids and manganese species). And the small manganese complexes play an important role in the antioxidative system of D. radiodurans. Recent studies have characterized several regulators (e.g., PprI and PprM) in D. radiodurans, which play critical roles in the protection of the bacteria from various stresses. In this review, we offer a panorama of the progress regarding the characteristics of the antioxidative system in D. radiodurans and its application in the future.
摘要：
Deinococcus radiodurans is a model microorganism used for studies on DNA repair and antioxidation due to its extraordinary tolerance to ionizing radiation and other DNA-damaging agents. Various transcriptome analyses have revealed that hundreds of genes are induced and that many other genes are repressed during recovery of D. radiodurans following irradiation, suggesting that gene regulation is of great importance for the extreme resistance of this microorganism to ionizing radiation. In this article, we focus on some reported strategies that are employed by D. radiodurans to regulate the genes implicated in its extreme tolerance to ionizing radiation for a comprehensive understanding of the reasons this bacterium can survive such extraordinary stress. We expect this review to shed light on potential radioprotective agents and applications for use in a range of fields.
摘要：
Deinococcus radiodurans has attracted a great interest in the past decades due to its extraordinary resistance to ionizing radiation and highly efficient DNA repair system. Recent studies indicated that pprM is a putative pleiotropic gene in D. radiodurans and plays an important role in radioresistance and antioxidation, but its underlying mechanisms are poorly elucidated. In this study, pprM mutation was generated to investigate resistance to desiccation and oxidative stress. The result showed that the survival of pprM mutant under desiccation was markedly retarded compared to the wild strain from day 7–28. Furthermore, knockout of pprM increases the intercellular accumulation of ROS and the sensibility to H2O2 stress in the bacterial growth inhibition assay. The absorbance spectrum experiment for detecting the carotenoid showed that deinoxanthin, a carotenoid that peculiarly exists in Deinococcus, was reduced in the pprM mutant in the pprM mutant. Quantitative real time PCR showed decreased expression of three genes viz. CrtI (DR0861, 50%),CrtB (DR0862, 40%) and CrtO (DR0093, 50%), which are involved in deinoxanthin synthesis, and of Dps (DNA protection during starving) gene (DRB0092) relevant to ion combining and DNA protection in cells. Our results suggest that pprM may affect antioxidative ability of D. radiodurans by regulating the synthesis of deinoxanthin and the concentration of metal ions. This may provide new clues for the treatment of antioxidants.
