RADIATION AND ENVIRONMENTAL BIOPHYSICS,2015年54(2):207-216 ISSN：0301-634X
[Liu, Fang; Wen, Ge-Bo; You, Yong] Key Laboratory of Tumor Cellular and Molecular Pathology, University of South China, College of Hunan Province, Hengyang, China;[Du, Ke-Jie; Lin, Ying-Wu] School of Chemistry and Chemical Engineering, University of South China, Hengyang, China;[Lin, Ying-Wu; Wen, Ge-Bo] Laboratory of Protein Structure and Function, University of South China, Hengyang, China;[Fang, Zhen] College of Chemistry and Materials Science, Anhui Normal University, Wuhu, China
[Wen, GB] Univ South China, Lab Prot Struct & Funct, Hengyang 421001, Peoples R China.
Caspases - Chemical and biologicals - Death receptor pathways - Dose-dependent manner - Hepatic cells - Mitochondrial membrane potential - Signaling pathways - Transmission electron
[Liu, Fang; Tang, Yunlian; Cheng, Ailan; Wu, Yimou; Zhang, Yang; Gan, Runliang] Univ S China, Canc Res Inst, Hengyang City 421001, Hunan, Peoples R China.;[He, Rongfang] Univ S China, Dept Pathol, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.
[Gan, RL] Univ S China, Canc Res Inst, Hengyang City 421001, Hunan, Peoples R China.
BACKGROUND: Epstein-Barr virus (EBV) has a close association with various types of human lymphomas. Animal models are essential to elucidate the pathogenesis of human EBV-associated lymphomas. The aim of the present study is to evaluate the association between human IgG concentration and EBV-associated lymphoma development in huPBL/SCID mice. METHODS: Human peripheral blood lymphocytes (hu-PBL) from EBV-seropositive donors were inoculated intraperitoneally into SCID mouse. Immunohistochemical staining was used to examine differentiated antigens of tumor cells. EBV infection of the induced tumors was detected by in situ hybridization. IgG concentrations in the serums of 12 SCID mice were measured by unidirectional immunodiffusion assay. RESULTS: 21 out of 29 mice developed tumors in their body. Immunohistochemical staining showed that all induced tumors were LCA (leukocyte common antigen) positive, B-cell markers (CD20, CD79a) positive, and T-cell markers (both CD3 and CD45RO) negative. The tumors can be diagnosed as human B-cell lymphomas by these morphological and immunohistochemical features. In situ hybridization exhibited resultant tumor cells had EBV encoded small RNA-1 (EBER-1). Human-derived IgG could be found in the serum from SCID mice on the 15th day following hu-PBL transplantation, and IgG levels increased with the tumor development in 6 hu-PBL/SCID chimeras. CONCLUSIONS: Intraperitoneal transfer of hu-PBLs from EBV+ donors to SCID mice leads to high human IgG levels in mouse serum and B cell lymphomas. Our findings suggest that increasing levels of human-derived IgG in peripheral blood from hu-PBL/SCID mice could be used to monitor EBV-related human B-cell lymphoma development in experimental animals.
The interaction of blood glucose with heme proteins plays a key role in inducing diabetes, a serious disease threatening human health. In this study, we investigated the non-covalent interaction between glucose and myoglobin (Mb), both theoretically and experimentally, using molecular dynamics (MD) simulation combined with spectroscopic studies. It revealed that glucoses can occupy the side pocket of Mb, and bind closely to one of the xenon cavities in Mb, by hydrogen bonding interactions with two propionate groups of heme as well as surrounding amino acids. These interactions alter the conformation of the heme active site slightly and lead to an enhanced peroxidase activity of Mb, as determined by kinetic studies. This study provides general information for glucose-heme proteins interactions, and also for blood glucose-protein interactions for patients with diabetes.