期刊论文

Upregulation of RORα suppresses target genes ofWnt/β-catenin pathway in human gastric MGC803 cells by DADS

中文

中国药理学通报

1001-1978

2017

33

4

522-528

10.3969/j.issn.1001-1978.2017.04.015

国家自然科学基金资助项目（No 81374013,81102854） 湖南省卫计委科研课题（No B2015-182）

本校为第一机构

目的 观察DADS上调RORα对人胃癌MGC803细胞Wnt/β-catenin通路相关分子与靶基因表达的影响.方法 RT-PCR、Western blot与免疫共沉淀检测Wnt/β-catenin通路相关分子与靶基因表达.荧光素酶报告基因检测c-Myc基因启动子活性.结果 RT-PCR 与Western blot显示,DADS处理后各组RORα mRNA与蛋白表达上调(P

Aim: In ground of prophase work, to investigate the upregulation of RORα suppresses target genes of Wnt/p-catenin pathway in human gastric MGC803 cells by DADS. Methods: The expressions of correlated molecules and target genes in Wnt/β-catenin pathway were tested by RT-PCR, Western blot and co-immunoprecipitation. Luciferase assay was used to assess the promoter activity of c-Myc. Results: Soft agar colony formation showed that the proliferation in silence group of RORa was significantly strengthened compared with in control group, and the pr...