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二烯丙基二硫上调RORα抑制人胃癌细胞Wnt/β-catenin通路靶基因

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成果类型:
期刊论文
作者:
苏坚;赵晓红;向姝霖;刘芳;夏红;...
作者机构:
Dept of Pathology, Second Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China
[夏红; Zeng X.; 苏琦; 刘芳; 向姝霖; 凌晖; 苏波] Cancer Research Institute, Center for Gastric Cancer Research of Hunan Province, Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan, Provincial University, University of South China, Hengyang, 421001, China
Hainan Maternal and Child Health Hospital, Haikou, 570206, China
[苏坚] Dept of Pathology, Second Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China, Cancer Research Institute, Center for Gastric Cancer Research of Hunan Province, Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan, Provincial University, University of South China, Hengyang, 421001, China
[赵晓红] Cancer Research Institute, Center for Gastric Cancer Research of Hunan Province, Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan, Provincial University, University of South China, Hengyang, 421001, China, Hainan Maternal and Child Health Hospital, Haikou, 570206, China
语种:
中文
关键词:
二烯丙基二硫;人胃癌细胞;Wnt/β-catenin信号通路;相关分子;靶基因;启动子活性
关键词(英文):
RORα
期刊:
中国药理学通报
ISSN:
1001-1978
年:
2017
卷:
33
期:
4
页码:
522-528
基金类别:
国家自然科学基金资助项目(No 81374013,81102854) 湖南省卫计委科研课题(No B2015-182)
机构署名:
本校为第一机构
院系归属:
医学院
摘要:
目的观察DADS上调RORα对人胃癌MGC803细胞Wnt/β-catenin通路相关分子与靶基因表达的影响。方法RT-PCR、Western blot与免疫共沉淀检测Wnt/β-catenin通路相关分子与靶基因表达。荧光素酶报告基因检测c-Myc基因启动子活性。结果 RT-PCR与Western blot显示,DADS处理后各组RORα mRNA与蛋白表达上调(P
摘要(英文):
Aim: In ground of prophase work, to investigate the upregulation of RORα suppresses target genes of Wnt/p-catenin pathway in human gastric MGC803 cells by DADS. Methods: The expressions of correlated molecules and target genes in Wnt/β-catenin pathway were tested by RT-PCR, Western blot and co-immunoprecipitation. Luciferase assay was used to assess the promoter activity of c-Myc. Results: Soft agar colony formation showed that the proliferation in silence group of RORa was significantly strengthened compared with in control group, and the pr...

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