二烯丙基二硫上调miR-22通过Wnt-1通路抑制人胃癌细胞增殖与迁移侵袭

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成果类型：

期刊论文

论文标题(英文)：

Up-regulation of miR-22 through Wnt pathway suppresses proliferation, migration and invasion in human gastric MGC803 cells by DADS

作者：

唐云云;唐仪;刘芳;苏坚;夏红;...

作者机构：

[Zeng X.; 夏红; 苏波; 刘芳; 苏琦] Cancer Research Institute, Center for Gastric Cancer Research of Hunan Province, Key Lab of Cancer Cellular and Molecular Pathology, Hunan Provincial University, University of South China, Hengyang Hunan, 421001, China

Dept of Basic Medicine, Yongzhou Vocational Technical College, Yongzhou Hunan, 425100, China

Dept of Pathology, Affiliated Xiangtan Hospital, University of South China, Xiangtan Hunan, 411101, China

Dept of Pathology, Second Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China

[唐云云] Cancer Research Institute, Center for Gastric Cancer Research of Hunan Province, Key Lab of Cancer Cellular and Molecular Pathology, Hunan Provincial University, University of South China, Hengyang Hunan, 421001, China, Dept of Basic Medicine, Yongzhou Vocational Technical College, Yongzhou Hunan, 425100, China

语种：

中文

关键词：

二烯丙基二硫;人胃癌细胞;Wnt通路;迁移;侵袭

关键词(英文)：

miR-22

期刊：

中国药理学通报

ISSN：

1001-1978

年：

2017

卷：

期：

页码：

1141-1147

DOI：

10.3969/j.issn.1001-1978.2017.08.020

基金类别：

No81374013:国家自然科学基金 81102854:国家自然科学基金 31100935:国家自然科学基金

机构署名：

本校为第一机构

院系归属：

医学院

摘要：

目的探讨二烯丙基二硫(diallyl disulfide,DADS)上调miR-22是否通过Wnt-1通路抑制人胃癌MGC803细胞增殖与迁移侵袭.方法 MTT、细胞划痕实验、侵袭实验分别检测DADS与miR-22对MGC803细胞增殖与迁移侵袭的影响.在线预测软件寻找miR-22调控的靶基因,荧光素酶报告基因检测miR-22对Wnt-1 3′UTR荧光酶活性的影响.qRT-PCR检测Wnt-1mRNA表达变化.Western blot检测Wnt-1、β-catenin与TCF-4蛋白表达.结果 MTT显示,DADS 与miR-22可明显抑制MGC803细胞增殖(P

摘要(英文)：

Aim: To investigate the up-regulation of miR-22 through Wnt pathway inhibits the proliferation, migration and invasion in human gastric MGC803 cells induced by diallyl disulfide (DADS). Methods: The effects of proliferation, migration, and invasion of gastric cancer cells were evaluated by MTT, wound-healing and invasion assays. Online prediction software was applied to search the target gene of miR-22. Luciferase report gene assay was used to assess the target genes Wnt-1 of miR-22. The expressions of Wnt-1,[3-cate-nin and TCF-4 were tested by...

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