期刊论文

The differential expression of microRNA in human gastric cancer MGC803 cells induced by diallyl disulfide

中文

中国药理学通报

1001-1978

2019

35

6

782-786

10.3969/j.issn.1001-1978.2019.06.010

No81374013:国家自然科学基金 31100935:国家自然科学基金 81641112:国家自然科学基金

本校为第一机构

目的研究二烯丙基二硫(diallyl disulfide,DADS)诱导人胃癌MGC803细胞的差异miRNAs表达。方法采用miRNAs芯片与qRT-PCR检测DADS诱导人胃癌MGC803细胞的差异miRNAs表达。结果microRNA芯片检测显示, 30 mg·L ~(- 1) DADS处理24 h后,MGC803细胞的差异miRNAs表达中,miR-200b、miR-22、miR-7、miR-143、miR- 138、miR-34a、miR-150等7个miRNA表达明显上调,miR- 222、miR-21、miR-15b、miR-182、miR-18a等5个miRNA下调。qPCR证实,30 mg·L ~(- 1) DADS处理MGC803细胞后, miR-200b、miR-22、miR-7、miR-143、miR-138、miR-34a、miR- 150等表达较未处理组明显上调(P < 0. 05) 。且qPCR检测显示,miR-200...

Aim: To investigate the expression and i-dentification of differential miRNAs in human gastric cancer MGC803 cells induced by diallyl disulfide (DADS). Methods: Differential miRNAs expression in human gastric cancer MGC803 cells induced by DADS was detected and identified by miRNA chip and qPCR. Results: MiRNAs chip detection showed upregulation of miR-200b, miR-22, miR-7, miR-143, miR-138, miR-34a and miR-150, and down-regulation of miR-222, miR-21, miR-15b, miR-182 and miR-18a in differential miRNAs of MGC803 cells treated with 3 0 mg · IT-1...