期刊论文

miR-548a-3p targeting MMP-2 inhibits the metastasis and invasion of gastric cancer cells

中文

实用医学杂志

1006-5725

2017

33

8

1235-1238

10.3969/j.issn.1006-5725.2017.08.011

衡阳市科技局课题资助项目（编号:2014KJ13）；

本校为第一机构

目的:研究miR-548a-3p靶向基质金属蛋白酶-2(MMP-2)抑制胃癌侵袭转移的分子机制.方法:通过生物信息学软件寻找可与MMP-2结合的miRNAs.qRT-PCR检测miR-548a-3p在不同分化和转移的胃癌细胞株中的表达,Western blot检测MMP-2表达;在三个重组细胞株中,qRT-PCR检测miR-548a-3p表达,Western blot检测MMP-2表达;双荧光素酶报告基因系统检测miR-548a-3p是否靶向结合MMP-2的3'-UTR端;Transwell实验检测转染前后胃癌细胞侵袭能力改变.结果:人低分化胃癌细胞株BGC-823中MMP-2高表达,而miR-548a-3p低表达;中、高分化人胃癌细胞株中MMP-2低表达,而miR-548a-3p高表达;双荧光素酶实验结果表明miR-548a-3p能靶向...

Objective To study the mechanism of miR-548a-3p targeting MMP-2 inhibits the metastasis and invasion of gastric cancer cells. Methods Bioinformatics were used to search those miRNAs targeting MMP-2. The level of miR-548a-3p in gastric cancer tissues were detected by qRT-PCR and the expression of MMP-2 were detected by Western blot. The level of miR-548a-3p in three reconstructed gastric cancer cell lines were detected by qRT-PCR and the expression of MMP-2 were detected by Western blot. miR-548a-3p targeting the 3＇-UTR of MMP-2 were detected b...