期刊论文

中文

中国实验血液学杂志

1009-2137

2018

26

3

750-755

10.7534/j.issn.1009-2137.2018.03.020

国家自然科学基金

本校为第一机构

目的:研究二烯丙基二硫(diallyl disulfide,DADS)下调MCL-1诱导人白血病K562细胞周期阻滞及其机制.方法:采用CCK-8检测DADS对K562细胞增殖的作用;流式细胞术观察DADS与沉默MCL-1对K562细胞周期的影响;Western blot检测MCL-1、PCNA和CDK1表达;免疫共沉淀方法分析MCL-1与PCNA及CDK1结合作用.结果: 15、30、60、120、240 μmol/L DADS对K562细胞增殖抑制率分别为32.48%、59.34%、66.42%、77.06%和81.05%(P

OBJECTIVE: To investigate the inducing effect of down-regulation of MCL-1 by diallyl disulfide(DADS) on the G2/M arrest of human leukemia K562 cells and its mechanisms. METHODS: CCK-8 was used to detect the effect of DADS on proliferation of K562 cells, flow cytometry was employed to observe the effect of cycle arrest by DADS and RNAi silencing MCL-1 gene in K562 cells. The expressions of MCL-1, PCNA and CDK1 in K562 cells treated with DADS were detected by Western blot. The amphigamy of MCL-1 with PCNA and CDK1 was detected by Coimmunoprecipitation. RESULTS: CCK-8 detection showed that the in...