期刊：
Life Sciences,2020年255(1):117830 ISSN：0024-3205
通讯作者：
Chen, Zhuo;Mo, Zhong-cheng
作者机构：
[Long, Shuang-lian; Zhu, Jing-Ling; Chen, Zhuo] Univ South China, Hunan Prov Innovat Training Base Med Postgrad, Yueyang 416000, Hunan, Peoples R China.;[Long, Shuang-lian; Zhu, Jing-Ling; Chen, Zhuo] Yueyang Women & Childrens Med Ctr, Yueyang 416000, Hunan, Peoples R China.;[Zhu, Jing-Ling; Cai, Ya-qin; Mo, Zhong-cheng] Guilin Med Univ, Inst Basic Med Sci, Ctr Diabet Syst Med, Guangxi Key Lab Excellence, Guilin 541199, Guangxi, Peoples R China.;[Zhu, Jing-Ling; Cai, Ya-qin] Univ South China, Hengyang Med Sch, Dept Histol & Embryol, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Hunan, Peoples R China.;[Mo, Zhong-cheng] Guilin Med Univ, Guilin 541199, Guangxi, Peoples R China.
通讯机构：
[Chen, Zhuo] Y;[Mo, Zhong-cheng] G;Yueyang Women & Childrens Med Ctr, Yueyang 416000, Hunan, Peoples R China.;Guilin Med Univ, Guilin 541199, Guangxi, Peoples R China.
关键词：
Advanced glycation end products (AGEs);DNA damage;Infertility;Ovulatory dysfunction;Oxidative stress
摘要：
National Strength and Conditioning Association Journal is the professional journal for strength coaches, personal trainers, athletic trainers, physical therapists, and other health professionals working in the strength and conditioning field. The journal's goal is to publish articles that report both the practical applications of previously published peer-reviewed research findings and the knowledge gained by experienced professionals. This mission includes, to the greatest extent possible, integrating these two sources of knowledge, providing practitioners with the most accurate information available, and providing a forum for the exchange of information between the many disciplines involved in strength and conditioning.Full text coverage in the Journals@Ovid database ranges from 1983 to 1993.
摘要：
Polycystic ovary syndrome (PCOS) is a complex and heterogeneous endocrine disease characterized by clinical or laboratorial hyperandrogenism, oligo-anovulation and metabolic abnormalities, including insulin resistance, excessive weight or obesity, type II diabetes, dyslipidemia and an increased risk of cardiovascular disease. The most significant clinical manifestation of PCOS is hyperandrogenism. Excess androgen profoundly affects granulosa cell function and follicular development via complex mechanisms that lead to obesity and insulin resistance. Most PCOS patients with hyperandrogenism have steroid secretion defects that result in abnormal folliculogenesis and failed dominant follicle selection. Hyperandrogenism induces obesity, hairy, acne, and androgenetic alopecia. These symptoms can bring great psychological stress to women. Drugs such as combined oral contraceptive pills, metformin, pioglitazone and low-dose spironolactone help improve pregnancy rates by decreasing androgen levels in vivo. Notably, PCOS is heterogeneous, and hyperandrogenism is not the only pathogenic factor. Obesity and insulin resistance aggravate the symptoms of hyperandrogenism, forming a vicious cycle that promotes PCOS development. Although numerous studies have been conducted, the definitive pathogenic mechanisms of PCOS remain uncertain. This review summarizes and discusses previous and recent findings regarding the relationship between hyperandrogenism, insulin resistance, obesity and PCOS.
期刊：
DNA AND CELL BIOLOGY,2020年39(8):1401-1409 ISSN：1044-5498
通讯作者：
Zhong, Qiaoqing;Mo, Zhongcheng
作者机构：
[Zeng, Xin; Long, Shuang Lian; Huang, Qin; Mo, Zhongcheng] Univ South China, Hengyang Med Sch, Clin Anat & Reprod Med Applicat Inst, Dept Histol & Embryol, Hengyang, Hunan, Peoples R China.;[Zhong, Qiaoqing] Cent South Univ, Xiangya Hosp, Dept Cardiovasc Med, Changsha 410008, Peoples R China.;[Mo, Zhongcheng] Guilin Med Univ, Inst Basic Med Sci, Guilin 541100, Guangxi, Peoples R China.
通讯机构：
[Zhong, Qiaoqing] C;[Mo, Zhongcheng] G;Cent South Univ, Xiangya Hosp, Dept Cardiovasc Med, Changsha 410008, Peoples R China.;Guilin Med Univ, Inst Basic Med Sci, Guilin 541100, Guangxi, Peoples R China.
关键词：
mitochondrion;reproductive metabolism;polycystic ovary syndrome;follicular development
摘要：
Polycystic ovary syndrome (PCOS) is one of the most common female reproductive metabolisms. It is an endocrine disease that affects reproductive women and often exhibits with hyperandrogenemia, insulin resistance (IR), low inflammation, and an increased risk of type 2 diabetes mellitus, metabolic syndrome, and cardiovascular events such as hypertension and dyslipidemia in patients. However, the molecular mechanism of PCOS is still unclear. Recently, an increasing number of studies have shown that the oxidative stress induced by mitochondrial dysfunction has negative effects on IR, lipid metabolism, and follicular development, suggesting that mitochondrial dysfunction plays an essential role in the development of PCOS. Abnormal mitochondrial DNA copy number in patients with PCOS, and mitochondrial gene mutations, has been the focus of research in recent years, and functional mitochondrial diseases have been gradually accepted as a related factor in PCOS. This review is intended to summarize and discuss previous and recent studies and findings on the connections between mitochondrial dysfunction and PCOS.
期刊：
CURRENT MOLECULAR MEDICINE,2019年19(10):719-730 ISSN：1566-5240
通讯作者：
Mo, Z.
作者机构：
[Yang, Zhen; Huang, Qin; Xie, Yuanjie; Mo, Zhongcheng] Clinical Anatomy & Reproductive Medicine Application Institute, Department of Histology and Embryology, Hengyang Medical school, University of South China, Hengyang 421001, China;[Liu, Yannan] Nursing School, Hunan University of Medicine, Huaihua 418000, China
通讯机构：
[Mo, Z.] C;Clinical Anatomy & Reproductive Medicine Application Institute, Department of Histology and Embryology, Hengyang Medical school, University of South China, Hengyang, China
关键词：
Ovarian follicles;lipoprotein;sterol carrier protein 2;steroidogenesis;cholesterol homeostasis;fertility.
期刊：
DNA AND CELL BIOLOGY,2019年38(8):754-762 ISSN：1044-5498
通讯作者：
Wu, Peng;Mo, Zhongcheng
作者机构：
[Wu, Hongliang; Ou, Hanxiao; Chen, Zhuo; Wu, Peng; Mo, Zhongcheng] Univ South China, Hunan Prov Innovat Training Base Med Postgrad, YueYang Maternal Child Med Hlth Hosp, Yueyang 414000, Hunan, Peoples R China.;[Wu, Hongliang; Ou, Hanxiao; Chen, Zhuo; Wu, Peng; Mo, Zhongcheng] Yueyang Women & Childrens Med Ctr, Yueyang 414000, Hunan, Peoples R China.;[Ou, Hanxiao; Mo, Zhongcheng] Univ South China, Hengyang Med Sch, Clin Anat & Reprod Med Applicat Inst, Dept Histol & Embryol, Hengyang, Hunan, Peoples R China.;[Mo, Zhongcheng] Univ South China, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wu, Peng; Mo, Zhongcheng] U;[Wu, Peng] Y;Univ South China, Hunan Prov Innovat Training Base Med Postgrad, YueYang Maternal Child Med Hlth Hosp, Yueyang 414000, Hunan, Peoples R China.;Yueyang Women & Childrens Med Ctr, Yueyang 414000, Hunan, Peoples R China.;Univ South China, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
摘要：
Polycystic ovary syndrome (PCOS) is the most typical metabolic syndrome in women of reproductive age, with a high prevalence and an increased risk of long-term complications. PCOS mainly manifests as hyperandrogenism (HA), ovulatory dysfunction, and polycystic ovaries, in addition to being relevant to infertility, insulin resistance (IR), obesity, lipid abnormalities, and chronic low-grade inflammation. The etiology of this syndrome remains largely unknown. microRNAs (miRNAs), small, noncoding RNAs (nearly 22 nucleotides long), regulate gene expression at the posttranscriptional level. Abnormal miRNA levels are closely associated with the occurrence of diseases, such as diabetes, cancers, and atherosclerosis, and miRNAs can be used as predictors and diagnostic biomarkers for cancer. Interestingly, the roles of miRNAs in PCOS pathology have attracted considerable attention in recent years. Research has established that alterations in miRNA expression in women with PCOS compared with healthy women may act as noninvasive biomarkers and new therapeutic targets in PCOS. This article aims to summarize the latest research on the relationship between miRNAs and the clinical manifestations of PCOS while also providing a few mechanisms based on previous studies. Understanding the relationship between miRNAs and PCOS will provide guidance for researchers to further explore the complexity and heterogeneity of PCOS.
摘要：
Polycystic ovary syndrome (PCOS), one of the most common endocrine diseases that causes infertility in reproductive women, is characterized by hyperandrogenemia, chronic anovulation, and polycystic ovary morphology (PCOM), and most women with PCOS have metabolic abnormalities. A reduction in plasma sex hormone-binding globulin (SHBG), a transport carrier that binds estrogen and androgens and regulates their biological activities, is often used as an indicator of hyperandrogenism in women with PCOS. Low serum SHBG levels are considered a biomarker of abnormal metabolism and are related to insulin resistance (IR), compensatory hyperinsulinemia and abnormalities in glucose and lipid metabolism in PCOS patients. SHBG is also associated with the long-term prognosis of PCOS. SHBG gene polymorphism is correlated with the risk of PCOS. As SHBG plays a vital role in the occurrence and development of PCOS, knowledge regarding its role in PCOS is helpful for further understanding the molecular mechanism of SHBG in PCOS development and providing new ideas for the treatment of female infertility. Hepatocyte nuclear factor-4alpha (HNF-4alpha) is a vital transcription factor in the SHBG synthesis process. HNF-4alpha binds to the cis-type element DR1 in the SHBG promoter to initiate transcription and regulates hepatic SHBG levels by modulating glucose and lipid metabolism and inflammatory factors. However, it remains unclear whether HNF-4alpha is indirectly involved in the pathogenesis of PCOS via regulation of hepatic SHBG synthesis. Therefore, this review discusses the interaction between SHBG and the various complications of PCOS as well as the regulatory effect of HNF-4alpha on SHBG expression.
摘要：
With the arrival of the precision medicine and personalized treatment era, targeted therapy that improves efficacy and reduces side effects has become the mainstream approach of cancer treatment. Antibody fragments that further enhance penetration and retain the most critical antigen-specific binding functions are considered the focus of research targeting cancer imaging and therapy. Thanks to the superior penetration and rapid blood clearance of antibody fragments, antibody fragment-based imaging agents enable efficient and sensitive imaging of tumour sites. In tumour-targeted therapy, antibody fragments can directly inhibit tumour proliferation and growth, serve as an ideal carrier for delivery of anti-tumour drugs, or manipulate the immune system to eliminate tumour cells. In this review, the excellent physicochemical properties and the basic structure of antibody fragments are expressly depicted depicted, the progress of antibody fragments in cancer therapy and imaging are thoroughly summarized, and the future development of antibody fragments is predicted.
作者机构：
[Zhang, Mengxia] Hunan Univ Chinese Med, Dept Histol & Embryol, Changsha 410208, Hunan, Peoples R China.;[Tang, Shengsong] Hunan Univ Med, Hunan Prov Key Lab Antibody Based Drug & Intellig, Huaihua 418000, Hunan, Peoples R China.;[Zhang, Mengxia; Liu, Qi; Mo, Zhongcheng] Univ South China, Clin Anat & Reprod Med Applicat Inst, Dept Histol & Embryol, Hengyang 421001, Hunan, Peoples R China.;[Tang, Shengsong; Lei, Xiaoyong; Tu, Jian; Li, Lijun] Univ South China, Insitute Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.;[Ning, Jing; Tang, Shengsong] Hunan Univ Med, Dept Pharmacol, Huaihua 418000, Hunan, Peoples R China.
通讯机构：
[Tang, Shengsong] H;Hunan Univ Med, Hunan Prov Key Lab Antibody Based Drug & Intellig, Huaihua 418000, Hunan, Peoples R China.
关键词：
macrophage colony-stimulating factor;breast cancer;apoptosis;HIF-1 and BINP3
摘要：
Macrophage colony-stimulating factor (M-CSF), a tumour marker, is related to tumour cell anti-apoptosis and drug resistance. However, the role of M-CSF in MCF-7 cells is unknown. In the present study, the effect and mechanism of M-CSF on hypoxia-inducible factor-1 (HIF-1)/BCL2/adenovirus E1B 19 kDa-interacting protein 3 (BNIP3)/Apoptosis Regulator BAX signalling in human breast cancer MCF-7 cells were investigated. Western blotting revealed that the expression of HIF-1, BNIP3, Bax, caspase-3 and caspase-9 was lower in MCF-7-M cells compared to MCF-7 and MCF-7-C cells treated with adriamycin (ADM). Immunoprecipitation combined with western blotting was used to detect the interaction between Bcl-2 and BNIP3 or Bax protein. MCF-7-M cells had a higher amount of Bax binding to Bcl-2 compared to MCF-7 cells or MCF-7-C cells, while the amount of BNIP3 binding to Bcl-2 was decreased in MCF-7-M cells. Hoechst 33342 staining and flow cytometry were utilized to evaluate the effect of M-CSF on apoptosis in MCF-7 cells treated with ADM. Compared to ADM-treated MCF-7 cells, the apoptotic rate of MCF-7-M cells was significantly decreased. These effects were dependent on the concentration of ADM. In conclusion, cytoplasmic M-CSF suppressed apoptosis by inhibiting the HIF-1/BNIP3/Bax signalling pathway, which potentiated the dissociation of Bcl-2 from Bcl-2-BNIP3 compounds and the formation of Bcl-2-Bax compounds.
