期刊论文

中文

实用医学杂志

1006-5725

2019

35

8

1233-1238+1245

10.3969/j.issn.1006-5725.2019.08.009

2018JJ2352:湖南省自然科学基金 2015JJ2128:湖南省自然科学基金 2017KJ182:实验室建设项目 2018KYY269:南华大学研究生科学基金项目 2018KYZ053:南华大学研究生科学基金项目

本校为第一机构

目的探讨棕榈酸经过氧化物酶体增殖物激活受体α（PPARα）对卵巢颗粒细胞脂质含量的影响。方法采用不同浓度的棕榈酸（PA）处理人卵巢颗粒细胞株（KGN）。经油红O和NBD-Cholesterol方法观察KGN细胞内脂质含量,Western blot、免疫细胞化学和免疫荧光检测B类I型清道夫受体（SR-BI）、PPARα的表达。结果不同浓度棕榈酸（0、0.000 1、0.000 2、0.000 3 mol/L）呈浓度依赖性促进KGN细胞脂质增加。不同浓度棕榈酸（0、0.000 1、0.000 2、0.000 3 mol/L）呈浓度依赖性上调KGN细胞SR-BI的表达（P

Objective In order to study the effects and mechanisms of palmitic acid(PA)on lipid content in human ovarian granulose cells(KGN).Methods Oil red O and NBD-Cholesterol were used to observe lipid content in KGN treatment with different concentrations of PA.The expressions of scavenger receptor class B type I(SR-BI)and peroxisome proliferators-activated receptorα(PPARα)were measured by Western blotting,Immunocytochemistry and Immunofluorescence.Results Palmitic acid(0,0.000 1,0.000 2,0.000 3 mol/L)promoted lipid increase in KGN cells in a conce...