五年制临床医学卓越医生教育培养中医学人文教育的探索
作者:
游咏;田英;桂庆军;沈元琼;李熠;...
期刊:
教育教学论坛 ,2014年(10):153-154 ISSN:1674-9324
作者机构:
南华大学 医学院 诊断学教研室,湖南 衡阳,421001;[李熠; 文格波; 沈元琼; 桂庆军; 尹凯; 游咏; 田英] 南华大学
关键词:
临床医学;卓越医生;医学人文
摘要:
卓越医生教育培养计划的目标是培养高素质医学人才。五年制临床医学本科教育是“5+3”培养模式的关键。本文基于医学人文教育在五年制临床医学卓越医生教育培养中的重要性,拟从课程体系、师资队伍、校园氛围及拓展式教学四个方面探索医学人文教育的实施策略。
语种:
中文
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提升师资队伍综合实力建设 促进卓越医生的培养
作者:
谢娟;尹凯;冯聚玲;程健琳;钟慧;...
期刊:
医学教育研究与实践 ,2014年(4):765-767 ISSN:2096-3181
作者机构:
南华大学医学院诊断学教研室,湖南衡阳,421001;南华大学预防医学与放射卫生实验中心,湖南衡阳,421001;[李熠; 程健琳; 沈元琼; 钟慧; 谢娟; 冯聚玲; 尹凯; 游咏] 南华大学
关键词:
卓越医生;师资队伍建设;综合实力
摘要:
卓越医生的培养是从培养卓越医学生做起,建设一支高素质的高校师资队伍是培养卓越医学生的根本保证。本文对提高师资队伍综合实力建设方面提出了几点建议,即提高人文素养,树立教师道德模范;多途径、全方位提高教师教学能力;着力解决教师的实际问题,创造良好的工作环境。
语种:
中文
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NLRP3炎性体与代谢性疾病的研究进展
作者:
Li Jin-Feng;Xie Di;He Ping-Ping;Tang Yan-Yan;Tu Yu-Lin* ;...
期刊:
生物化学与生物物理进展 ,2014年41(5):425-434 ISSN:1000-3282
通讯作者:
Tu Yu-Lin
作者机构:
[Li Jin-Feng; Xie Di; Tu Yu-Lin; Yin Kai; Tang Yan-Yan; He Ping-Ping] Univ South China, Inst Cardiovasc Dis, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[He Ping-Ping] Univ South China, Sch Nursing, Hengyang 421001, Peoples R China.;[Yin Kai] Univ South China, Hunan Univ Chinese Med, Dept Tradit Chinese Diagnost, Hengyang 421001, Peoples R China.
通讯机构:
[Tu Yu-Lin] U;Univ South China, Inst Cardiovasc Dis, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.
关键词:
NLRP3炎性体;代谢性疾病;炎性因子
摘要:
代谢性疾病是由体内氨基酸、葡萄糖和脂质代谢紊乱引起的一类疾病,慢性炎症反应是其重要特征之一.Nod 样受体蛋白3(Nod-like receptor protein 3,NLRP3)炎性体是位于细胞内的一种蛋白质复合体,主要功能为活化半胱氨酸天冬氨酸蛋白酶1(caspase-1)以间接调控白介素1β(IL-1β)、IL-18和IL-33等的成熟和分泌.NLRP3炎性体是炎性体相关研究的热点,多种内源性或外源性危险信号通过激活这一蛋白质复合体上调炎性因子的表达水平,从而促进多种代谢性疾病的发生发展.本文对NLRP3炎性体的结构、功能、调节以及在代谢性疾病中的作用做一综述,以期为代谢性疾病的防治提供新靶点.
语种:
中文
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Apelin-13增加ABCA1蛋白水平调节PC-12细胞Aβ代谢
作者:
欧阳新平;何平平;吕运成;赵国军;莫中成;...
作者机构:
[欧阳新平; 何平平; 吕运成; 赵国军; 莫中成; 尹凯; 唐艳艳; 张敏; 谢巍; 唐朝克] 南华大学医学院生理学教研室、神经科学研究所;[欧阳新平; 何平平; 吕运成; 赵国军; 莫中成; 尹凯; 唐艳艳; 张敏; 谢巍; 唐朝克] 南华大学医学院心血管疾病研究所、生命科学研究中心
会议名称:
中国生理学会第24届全国会员代表大会暨生理学学术大会
会议时间:
2014-10-24
会议地点:
上海
会议论文集名称:
中国生理学会第24届全国会员代表大会暨生理学学术大会论文集
关键词:
Apelin-13;ABCA1;Aβ
摘要:
目的:观察apelin-13是否通过影响ABCA1蛋白水平从而调节Aβ代谢。方法:1Western blot检测PC-12细胞血管紧张素受体AT1相关的受体蛋白(putative recep tor p rotein related to the angiotensin recep tor AT1,APJ)表达,以确定PC-12细胞存在apelin-13作用受体。2用不同浓度(1、10、100nm
语种:
中文
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基于卓越医生教育培养计划的五年制临床医学人才培养模式的思考与实践
作者:
尹凯;谢娟;李熠;沈元琼;游咏;...
期刊:
医学教育研究与实践 ,2014年(3):442-443,496 ISSN:2096-3181
作者机构:
南华大学医学院诊断学教研室,湖南衡阳,421001;[李熠; 沈元琼; 桂庆军; 谢娟; 冯聚玲; 尹凯; 游咏] 南华大学
关键词:
卓越医生;五年制;临床医学;模式;思考
摘要:
我国卫生事业的发展和医药卫生体制改革对高等医学教育提出了更高要求,卓越医生教育培养计划是为提高医学人才培养质量而实施的改革举措之一。作者针对五年制临床医学人才卓越医生教育培养计划的内涵、目标、培养体系、培养方案和实践途径等方面进行探索和思考,为计划的实施提供了理论基础。
语种:
中文
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脂联素减轻高糖诱导的人血管内皮细胞系损伤
作者:
李熠;匡双玉;尹凯;匡稳定;桂庆军
期刊:
基础医学与临床 ,2014年34(10):1386-1390 ISSN:1001-6325
作者机构:
[李熠; 尹凯; 桂庆军] 南华大学医学院诊断学教研室;[匡双玉; 匡稳定] 南华大学医学院附属第二医院
关键词:
D-葡萄糖;人脐静脉内皮细胞;脂联素
摘要:
目的观察脂联素(APN)对高糖诱导血管内皮细胞系损伤的保护作用及NF-κB与LOX-1蛋白表达在其中的作用。方法30 mmol/LD-葡萄糖作用于HUVEC-12(人脐静脉内皮细胞系),不同浓度的脂联素(10、20和40 μg/mL)作用48 h及20 μg/mL脂联素作用不同时间(12、24、48和72 h)后,倒置相差显微镜观察内皮细胞形态,Western blot检测核转录因子NF-KB和LOX-1蛋白表达,间接免疫荧光法检测NF-KB核转位情况。结果脂联素作用于D-葡萄糖诱导的HUVEC-12,内皮细胞形态改善,折光性增强,胞内颗粒物减少,细胞排列相对规整;同时,LOX-1蛋白表达下调(P <0. 05),NF-κB活性降低(P<0.05),并呈现时间和浓度依赖性(n =3,P <0. 05)。结论脂联素可能通过NF-KB 信号通路引起LOX-1蛋白表达下调,从而发挥其对高糖诱导血管内皮细胞损伤的保护作用。
语种:
中文
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锌指蛋白去磷酸化在载脂蛋白AⅠ抑制脂多糖诱导的泡沫细胞炎症因子表达中的作用
作者:
李金凤;涂玉林;尹凯
期刊:
中国动脉硬化杂志 ,2014年22(3):233-236 ISSN:1007-3949
作者机构:
[李金凤; 涂玉林; 尹凯] 南华大学心血管疾病研究所
关键词:
锌指蛋白;载脂蛋白AⅠ;巨噬细胞源性泡沫细胞;炎症因子
摘要:
目的观察载脂蛋白AⅠ(ApoAⅠ)对脂多糖诱导的巨噬细胞源性泡沫细胞炎症因子表达的影响,探讨锌指蛋白(TTP)翻译后修饰在ApoAⅠ抑制泡沫细胞炎症因子表达中的作用。方法THP-1巨噬细胞源性泡沫细胞以 ApoAⅠ和/或脂多糖处理,采用ELISA 检测细胞裂解液中炎症因子白细胞介素1β(IL-1β)蛋白表达; 采用实时定量 PCR 检测IL-1β mRNA 表达和降解率; 采用Western blot 检测TTP 和磷酸化TTP(p-TTP)的表达。结果ApoAⅠ明显抑制脂多糖诱导泡沫细胞炎症因子的表达,并影响TTP 表达和TTP 去磷酸化。结论ApoAⅠ抑制泡沫细胞炎症因子表达机制涉及TTP 的去磷酸化,与TTP 促进含有腺苷酸环尿苷酸丰富的元件(ARE)的mRNA 降解有关。
语种:
中文
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Apolipoprotein A-I inhibits LPS-induced atherosclerosis in ApoE~(–/–) mice possibly via activated STAT3-mediated upregulation of tristetraprolin
作者:
Yin, Kai;Tang, Shi-lin;Yu, Xiao-hua;Tu, Guang-hui;He, Rong-fang;...
期刊:
中国药理学报 ,2013年34(6):837-846 ISSN:1671-4083
通讯作者:
Tu, Jian
作者机构:
[Tang, Chao-ke; Jiang, Zhi-sheng; Yu, Xiao-hua; Yin, Kai; Xie, Di; Li, Jin-feng; Tang, Shi-lin] Univ South China, Life Sci Res Ctr, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang 421001, Peoples R China.;[Gui, Qing-jun; Yin, Kai; Xie, Di] Univ South China, Coll Med, Dept Diagnost, Hengyang 421001, Peoples R China.;[Tang, Shi-lin] Univ South China, Affiliated Hosp 1, Dept Intens Care Unit, Hengyang 421001, Peoples R China.;[Tu, Guang-hui] Nan Xian Peoples Hosp, Dept Pathol, Yiyang 413200, Peoples R China.;[He, Rong-fang] Univ South China, Affiliated Hosp 1, Dept Pathol, Hengyang 421001, Peoples R China.
通讯机构:
[Tu, Jian] U;Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
关键词:
apolipoprotein A-I;heart;atherosclerosis;inflammation;cytokines;JAK2/STAT3 signaling pathway;tristetraprolin;lipopolysaccharide;ApoE-/-mice;macrophage;apolipoprotein A-I;heart;atherosclerosis;inflammation;cytokines;JAK2/STAT3 signaling pathway;tristetraprolin;lipopolysaccharide;ApoE-/-mice;macrophage
摘要:
Aim:To investigate the effects of the major component of high-density lipoprotein apolipoprotein A-I (apoA-I) on the development of atherosclerosis in LPS-challenged ApoE -/- mice and the underlying mechanisms.Methods: Male ApoE-KO mice were daily injected with LPS (25 μg, sc) or PBS for 4 weeks. The LPS-challenged mice were intravenously injected with rAAV-apoA-I-GFP or rAAV-GFP. After the animals were killed, blood, livers and aortas were collected for biochemical and histological analyses. For ex vivo experiments, the abdominal cavity macrophages were harvested from each treatment group of mice, and cultured with autologous serum, then treated with LPS.Results:Chronic administration of LPS in ApoE -/- mice significantly increased the expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, and MCP-1), increased infiltration of inflammatory cells, and enhanced the development of atherosclerosis. In LPS-challenged mice injected with rAAV-apoA-I-GFP, viral particles and human apoA-I were detected in the livers, total plasma human apoA-I levels were grammatically increased; HDL-cholesterol level was significantly increased, TG and TC were slightly increased. Furthermore, overexpression of apoA-I significantly suppressed the expression of proinflammatory cytokines, reduced the infiltration of inflammatory cells, and decreased the extent of atherosclerotic lesions. Moreover, overexpression of apoA-I significantly increased the expression of the cytokine mRNA-destabilizing protein tristetraprolin (TTP), and phosphorylation of JAK2 and STAT3 in aortas. In ex vivo mouse macrophages, the serum from mice overexpressing apoA-I significantly increased the expression of TTP, accompanied by accelerated decay of mRNAs of the inflammatory cytokines.Conclusion:ApoA-I potently suppresses LPS-induced atherosclerosis by inhibiting the inflammatory response possibly via activation of STAT3 and upregulation of TTP. © 2013 CPS and SIMM.
语种:
英文
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《临床技能学》国家精品课程建设的研究与实践
作者:
李熠;沈元琼;桂庆军;文格波;游咏;...
期刊:
教育教学论坛 ,2013年(42):167-169 ISSN:1674-9324
作者机构:
南华大学 医学院,湖南 衡阳,421001;[格波; 李熠; 沈元琼; 桂庆军; 尹凯; 游咏] 南华大学
关键词:
临床技能学;国家精品课程;研究与实践
摘要:
国家精品课程建设对提高高校教育教学以及人才培养质量具有重要的作用。通过探索临床技能学培养新模式、推进立体化教学资源建设、改革创新教学方法和手段以及加强硬件环境的建设等措施,为提高国家级精品课程建设的质量和水平提供新的思路和方向。
语种:
中文
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南华大学五年制临床医学专业人才培养方案的优化与实践
作者:
田英;尹凯;桂庆军;张新华;唐志晗;...
期刊:
中华医学教育探索杂志 ,2013年(9):875-878 ISSN:2095-1485
作者机构:
南华大学医学院, 衡阳,421001
关键词:
五年制;临床医学;培养方案
摘要:
随着我国卫生事业的发展,原有的临床医学专业人才培养方案已不能适应社会对人才的更高要求,构建新的人才培养方案成为高等医学院校教学改革的重要内容。现以南华大学为例,从人才培养目标、课程体系、实践环节和教学内容等方面分析五年制临床医学专业人才培养方案的变化趋势,对进一步优化临床医学专业人才培养方案提出思考。
语种:
中文
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Antagonism of Betulinic Acid on LPS-Mediated Inhibition of ABCA1 and Cholesterol Efflux through Inhibiting Nuclear Factor-kappaB Signaling Pathway and miR-33 Expression
作者:
Zhao, Guo-Jun;Tang, Shi-Lin;Lv, Yun-Cheng;Ouyang, Xin-Ping;He, Ping-Ping;...
期刊:
PLOS ONE ,2013年8(9):e74782 ISSN:1932-6203
通讯作者:
Yin, Kai
作者机构:
[Yin, Kai; He, Ping-Ping; Ouyang, Xin-Ping; Chen, Wu-Jun; Zhang, Min; Zhao, Guo-Jun; Lv, Yun-Cheng; Tang, Chao-Ke; Lu, Qian; Tang, Yan-Yan; Yao, Feng; Tang, Shi-Lin] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang, Hunan, Peoples R China.;[Zhao, Guo-Jun] Univ South China, Dept Histol & Embryol, Hengyang, Hunan, Peoples R China.;[He, Ping-Ping] Univ South China, Sch Nursing, Hengyang, Hunan, Peoples R China.;[Fu, Yuchang] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 USA.;[Zhang, Da-Wei] Univ Alberta, Dept Pediat, Edmonton, AB, Canada.
通讯机构:
[Yin, Kai] U;Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang, Hunan, Peoples R China.
关键词:
Cholesterol;Transcription factors;Macrophages;Atherosclerosis;Inflammation;Phosphorylation;MicroRNAs;Inflammatory diseases
摘要:
ATP-binding cassette transporter A1 (ABCA1) is critical in exporting cholesterol from macrophages and plays a protective role in the development of atherosclerosis. The purpose of this study was to investigate the effects of betulinic acid (BA), a pentacyclic triterpenoid, on ABCA1 expression and cholesterol efflux, and to further determine the underlying mechanism. BA promoted ABCA1 expression and cholesterol efflux, decreased cellular cholesterol and cholesterol ester content in LPS-treated macrophages. Furthermore, we found that BA promoted ABCA1 expression via down-regulation of miR-33s. The inhibition of LPS-induced NF-kappa B activation further decreased miR-33s expression and enhanced ABCA1 expression and cholesterol efflux when compared with BA only treatment. In addition, BA suppressed I kappa B phosphorylation, p65 phosphorylation and nuclear translocation, and the transcription of NF-kappa B-dependent related gene. Moreover, BA reduced atherosclerotic lesion size, miR-33s levels and NF-kappa B activation, and promoted ABCA1 expression in apoE(-/-) mice. Taken together, these results reveal a novel mechanism for the BA-mediated ABCA1 expression, which may provide new insights for developing strategies for modulating vascular inflammation and atherosclerosis.
语种:
英文
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大临床技能教学模式在医学人才培养中的应用探讨
作者:
桂庆军;游咏;尹凯;李熠;沈元琼;...
期刊:
医学教育研究与实践 ,2013年21(3):460-461,468 ISSN:2096-3181
作者机构:
南华大学医学院诊断学教研室,湖南衡阳,421002;[李熠; 文格波; 沈元琼; 桂庆军; 尹凯; 游咏] 南华大学
关键词:
临床技能教学模式;医学人才;培养
摘要:
临床技能教学是临床医学教学中的核心内容之一,是高等医学教育的重要组成部分。建立贴近临床,贴近应用的科学、系统的大,临床技能教学模式,更符合现代医学教育要求,能确实提高医学生临床技能,使之能尽快适应现实医疗环境。符合现代医学模式下医学人才培养的改革思路。可为临床技能教学模式改革提供借鉴。
语种:
中文
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Foam cells in atherosclerosis
作者:
Yu, Xiao-Hua;Fu, Yu-Chang;Zhang, Da-Wei;Yin, Kai* ;Tang, Chao-Ke
期刊:
Clinica Chimica Acta ,2013年424:245-252 ISSN:0009-8981
通讯作者:
Yin, Kai
作者机构:
[Tang, Chao-Ke; Yu, Xiao-Hua] Univ South China, Life Sci Res Ctr, Hengyang 421001, Hunan, Peoples R China.;[Tang, Chao-Ke; Yin, Kai] Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.;[Fu, Yu-Chang] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 USA.;[Zhang, Da-Wei] Univ Alberta, Dept Pediat, Edmonton, AB T6G 2S2, Canada.;[Zhang, Da-Wei] Univ Alberta, Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada.
通讯机构:
[Yin, Kai] U;Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词:
Foam cells;Atherosclerosis;CD36;ACAT1;ABCA1;ABCG1
摘要:
Atherosclerosis is a chronic disease characterized by the deposition of excessive cholesterol in the arterial intima. Macrophage foam cells play a critical role in the occurrence and development of atherosclerosis. The generation of these cells is associated with imbalance of cholesterol influx, esterification and efflux. CD36 and scavenger receptor class A (SR-A) are mainly responsible for uptake of lipoprotein-derived cholesterol by macrophages. Acyl coenzyme A:cholesterol acyltransferase-1 (ACAT1) and neutral cholesteryl ester hydrolase (nCEH) regulate cholesterol esterification. ATP-binding cassette transporters A1(ABCA1), ABCG1 and scavenger receptor BI (SR-BI) play crucial roles in macrophage cholesterol export. When inflow and esterification of cholesterol increase and/or its outflow decrease, the macrophages are ultimately transformed into lipid-laden foam cells, the prototypical cells in the atherosclerotic plaque. The aim of this review is to describe what is known about the mechanisms of cholesterol uptake, esterification and release in macrophages. An increased understanding of the process of macrophage foam cell formation will help to develop novel therapeutic interventions for atherosclerosis. © 2013 The Authors.
语种:
英文
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Posttranscriptional regulation of ATP-binding cassette transporter a1 in lipid metabolism
作者:
Lv, Yun-cheng;Yin, Kai;Fu, Yu-chang;Zhang, Da-wei;Chen, Wu-jun;...
期刊:
DNA AND CELL BIOLOGY ,2013年32(7):348-358 ISSN:1044-5498
通讯作者:
Tang, Chao-ke
作者机构:
[Tang, Chao-ke; Lv, Yun-cheng; Yin, Kai; Chen, Wu-jun] Univ South China, Life Sci Res Ctr, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Lv, Yun-cheng] Univ South China, Lab Clin Anat, Hengyang 421001, Peoples R China.;[Fu, Yu-chang] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 USA.;[Zhang, Da-wei] Univ Alberta, Dept Pediat, Edmonton, AB, Canada.;[Zhang, Da-wei] Univ Alberta, Grp Mol & Cell Biol Lipids, Edmonton, AB, Canada.
通讯机构:
[Tang, Chao-ke] U;Univ South China, Life Sci Res Ctr, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.
摘要:
Aim: Several lines of evidence have shown that posttranscriptional regulations play an important role in the modulation of ATP-binding cassette transporter A1 (ABCA1) expression and function. Results: The clearance of ABCA1 mRNA as well as the trafficking, stability, degradation, and activity of the ABCA1 protein are regulated by diverse posttranscriptional mechanisms. ABCA1 mRNA clearance is induced by several microRNAs that result in translational repression and reduction of ABCA1 protein expression. Intracellular ABCA1 trafficking is enhanced toward the plasma membrane, leading to an elevation of cell-surface localization, where the majority of the cholesterol efflux activity occurs. The ABCA1 protein turnover is rapid via calpain-mediated degradation and ubiquitin-mediated degradation. Various modulators retard ABCA1 protein clearance, which raises ABCA1 protein levels. The activity of ABCA1 can also be altered by a few molecules that do not affect ABCA1 protein expression. Conclusion: In this review, we summarize the advances in the knowledge of ABCA1 posttranscriptional regulation, which is warranted to better understand the role of ABCA1 in reverse cholesterol transport, lipid metabolism, and atherosclerosis. © 2013 Mary Ann Liebert, Inc.
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英文
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PHF-2在氧化型低密度脂蛋白诱导THP-1源性巨噬细胞核因子кB激活中的作用
作者:
涂光辉;李金凤;谢笛;桂庆军;唐朝克;...
期刊:
中国动脉硬化杂志 ,2013年21(9):780-784 ISSN:1007-3949
作者机构:
南县人民医院病理科,湖南省益阳市;南华大学医学院诊断学教研室,湖南省衡阳市421001
会议名称:
第十二次全国动脉硬化性疾病学术会议
会议时间:
2013-10-11
会议地点:
江苏苏州
会议论文集名称:
第十二次全国动脉硬化性疾病学术会议论文集
关键词:
氧化型低密度脂蛋白;巨噬细胞;植物同源结构域指蛋白2;炎症因子
摘要:
目的:动脉粥样硬化(atherosclerosis ,As)是一种炎症疾病,多种炎症因子参与了As的发生发展川。低密度脂蛋白(low density lipoprotein, LDL)是促进As进展的主要危险因素。观察氧化型低密度脂蛋白对THP-1巨噬细胞植物同源结构域指蛋白2(PHF-2)表达的影响,探讨表观遗传调节在氧化型低密度脂蛋白诱导巨噬细胞炎症反应中的作用.
方法:用不同浓度(0、50、100、150mg/L)氧化型低密度脂蛋白处理THP-1巨噬细胞4h,采用酶联免疫吸附法检测肿瘤坏死因子α和巨噬细胞炎性蛋白1β的表达;采用实时荧光定量聚合酶链反应和Western blot分别检测PHF-2mRNA和蛋白质的表达;采用免疫共沉淀法检测PHF-2和p65核因子κB的结合.
结果:100~150mg/L氧化型低密度脂蛋白处理THP-1巨噬细胞4h后,肿瘤坏死因子α和巨噬细胞炎性蛋白1β的表达明显上调;氧化型低密度脂蛋白呈浓度依赖性上调THP-1巨噬细胞PHF-2mRNA和蛋白质的表达,并促进PHF-2和p65核因子κB的结合.
结论:PHF-2参与氧化型低密度脂蛋白诱导THP-1巨噬细胞核因子κB激活和炎症因子表达.
语种:
中文
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亲环素A对氧化型低密度脂蛋白诱导的RAW264.7细胞中NF-κB蛋白表达的影响
作者:
许选选;尹凯;李涛;李金凤;涂剑;...
期刊:
中国动脉硬化杂志 ,2013年21(4):311-314 ISSN:1007-3949
作者机构:
南华大学心血管疾病研究所动脉硬化学湖南省重点实验室,湖南省衡阳市,421001;南华大学药物药理研究所,湖南省衡阳市,421001
关键词:
亲环素A;氧化型低密度脂蛋白;核因子κB
摘要:
目的 观察亲环素A对氧化型低密度脂蛋白诱导的RAW264.7细胞中核因子κB (NF-κB)表达的影响.方法 Western blot检测氧化型低密度脂蛋白处理RAW264.7细胞不同时间亲环素A和NF-κB蛋白的表达,siRNA沉降亲环素A后再用Western blot检测氧化型低密度脂蛋白对RAW264.7细胞中NF-κB蛋白表达的影响.结果 氧化型低密度脂蛋白能够上调RAW264.7细胞中亲环素A和NF-κB蛋白的表达,siRNA沉降亲环素A能降低细胞中NF-κB蛋白的表达.结论 亲环素A表达的改变能影响氧化型低密度脂蛋白诱导的RAW264.7细胞中NF-κB的蛋白表达.
语种:
中文
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现代教育技术在医学生临床基本技能教学中的应用探讨
作者:
梁丽红;桂庆军;文格波;沈元琼;尹凯;...
期刊:
医学教育研究与实践 ,2013年21(3):561-563 ISSN:2096-3181
作者机构:
南华大学医学院,湖南衡阳,421001;[李熠; 唐志晗; 沈元琼; 文格波; 桂庆军; 尹凯; 游咏; 梁丽红] 南华大学
关键词:
现代教育技术;临床基本技能;立体化教学
摘要:
通过探讨基础仿真模拟技术、高级综合模拟人技术、多媒体教学技术、计算机辅助教学、网络教学等现代教育技术的特点,分析各种现代教育技术手段教学方法的优缺点,提出通过优化组合各技术手段方法,可以实现优势互补,相辅相成,从而建立临床基本技能的立体化教学模式,提高教学效果。
语种:
中文
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Tertiary-butylhydroquinone upregulates expression of ATP-binding cassette transporter A1 via nuclear factor E2-related factor 2/heme oxygenase-1 signaling in THP-1 macrophage-derived foam cells
作者:
Lu, Qian;Tang, Shi-Lin;Liu, Xiao-Yan;Zhao, Guo-Jun;Ouyang, Xin-Ping;...
期刊:
Circulation Journal ,2013年77(9):2399-2408 ISSN:1346-9843
通讯作者:
Tang, Chao-Ke
作者机构:
[Yin, Kai; Liu, Xiao-Yan; He, Ping-Ping; Ouyang, Xin-Ping; Chen, Wu-Jun; Zhang, Min; Zhao, Guo-Jun; Lv, Yun-Cheng; Tang, Chao-Ke; Lu, Qian; Tang, Yan-Yan; Yao, Feng; Tang, Shi-Lin] Life Sci Res Ctr, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang, Hunan, Peoples R China.;[Tang, Shi-Lin] Univ South China, Affiliated Hosp 1, Dept Intens Care Unit, Hengyang 421001, Hunan, Peoples R China.;[He, Ping-Ping] Univ South China, Sch Nursing, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Da-Wei] Univ Alberta, Dept Pediat, Edmonton, AB, Canada.;[Zhang, Da-Wei] Univ Alberta, Grp Mol & Cell Biol Lipids, Edmonton, AB, Canada.
通讯机构:
[Tang, Chao-Ke] U;Univ South China, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词:
Atherosclerosis;ATP-binding cassette transporter A1 (ABCA1);Heme oxygenase-1 (HO-1);Nuclear factor E2-related factor 2 (Nrf2);Tert-butylhydroquinone (tBHQ)
摘要:
Tert-butylhydroquinone (tBHQ), a synthetic phenolic antioxidant, is commonly used as a food preservative because of its potent antilipid peroxidation activity. Several lines of evidence have demonstrated that dietary supplementation with antioxidants has an antiatherogenic function through reducing cholesterol uptake or promoting reverse cholesterol transport. In this study, we investigated whether tBHQ affects expression of ATP-binding cassette transporter A1 (ABCA1) and the potential subsequent effect on cellular cholesterol homeostasis. Methods and Results: tBHQ increased ABCA1 protein levels and markedly enhanced cholesterol efflux from THP-1 macrophage-derived foam cells. Furthermore, tBHQ reduced calpain-mediated ABCA1 proteolysis via activation of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). Inhibition of HO-1 with a pharmacological inhibitor or siRNA and knockdown of Nrf2 suppressed the stimulatory effects of tBHQ on ABCA1 expression and calpain activity. Conclusions: Nrf2/HO-1 signaling is required for the regulation by tBHQ of ABCA1 expression and cholesterol efflux in macrophage-derived foam cells and an antiatherogenic role of tBHQ is suggested.
语种:
英文
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AT1R基因A1166C多态性与急性心肌梗死的相关性研究
作者:
彭建业;张玲;尹凯;桂庆军
期刊:
现代诊断与治疗 ,2012年23(12):2059-2062 ISSN:1001-8174
作者机构:
南华大学附属第二医院心内科,湖南衡阳,421001;南华大学医学院诊断学教研室,湖南衡阳,421001;[彭建业; 张玲] 南华大学第二附属医院;[桂庆军; 尹凯] 南华大学
关键词:
血管紧张素Ⅱ1型受体(AT1R);基因多态性;急性心肌梗死
摘要:
目的 研究AT1R基因A1166C多态性与急性心肌梗死(AMI)的相关性.方法 采用PCR-RFLP方法,对105例初发AMI患者和111例健康对照个体进行ATIR基因A1166C多态性分析.结果 AT1R基因A1166C多态性中AA和AC+CC基因型在AMI患者组的频率分别为88.6%和11.4%,等位基因A和C的频率分别为93.3%和6.7%; AA和AC+CC基因型在对照组中的频率分别为92.8%和7.2%,等位基因A和C的频率分别为95.9%和4.1%.AMI组与对照组比较,AA和AC+CC基因型频率及A和C两等位基因频率均无统计学显著性差异(P>0.05).Logistic回归分析显示高血压、冠状动脉疾病家族史、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇是AMI发生的独立危险因素(P<0.05),高密度脂蛋白胆固醇是保护性因素(OR<1,95%CI 0.155~0.931),其余三项为危险因素(0R>1).结论 AT1R基因A1166C多态性与AMI的发生可能无关联.
语种:
中文
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Apolipoprotein A-I Inhibits CD40 Proinflammatory Signaling via ATP-Binding Cassette Transporter A1-Mediated Modulation of Lipid Raft in Macrophages
作者:
Yin, Kai;Chen, Wu-Jun;Zhou, Zhi-Gang;Zhao, Guo-Jun;Lv, Yun-Chen;...
期刊:
Journal of Atherosclerosis and Thrombosis ,2012年19(9):823-836 ISSN:1340-3478
通讯作者:
Tang, Chao-Ke
作者机构:
[Zhao, Guo-Jun; Jiang, Zhi-Sheng; Tang, Chao-Ke; Lv, Yun-Chen; Ouyang, Xin-Pin; Yu, Xiao-Hua; Yin, Kai; Chen, Wu-Jun; Zhou, Zhi-Gang] Univ S China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr, Hengyang 421001, Hunan, Peoples R China.;[Yin, Kai] Univ S China, Coll Med, Dept Diagnost, Hengyang 421001, Hunan, Peoples R China.;[Zhou, Zhi-Gang] Univ S China, Affiliated Hosp 1, Dept Anesthesiol, Hengyang 421001, Hunan, Peoples R China.;[Fu, Yuchang] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 USA.
通讯机构:
[Tang, Chao-Ke] U;Univ S China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr, Hengyang 421001, Hunan, Peoples R China.
关键词:
Apolipoprotein A-I;ATP-binding cassette transporter A1;CD40;Lipid raft
摘要:
AIM: Apolipoprotein A-I (apoA-I), the major component of high-density lipoprotein (HDL), has been recently found to suppress inflammation. This study was to investigate the effects and potential mechanisms of apoA-I on the CD40/CD40 ligand (CD40L) proinflammatory signaling pathway. METHODS: Human THP-1 macrophage-derived foam cells were treated with sCD40L alone or in the presence of apoA-I. Secretion of proinflammatory cytokines was performed by enzyme-linked immunosorbent assay(ELISA). The proteins and mRNA expression were examined by western-blot and real-time PCR analysis, respectly. Cholesterol efflux was assessed by liquid scintillation counting. Cholesterol depletion of macrophages was performed with methylated beta-cyclodextrin. RESULTS: ApoA-I inhibits the inflammatory response stimulated by soluble CD40L (sCD40L) in macrophages. In addition, apoA-I inhibited the sCD40L-stimulated activation of nuclear factor-kB (NF-kB). The apoA-I-induced NF-kB deactivation was related to the decreased recruitment of tumor necrosis factor receptor-associated factor 6 (TRAF-6), a crucial adapter protein for CD40 in macrophages, to lipid rafts after being treated by sCD40L. When interfering the expression of ATP-binding cassette transporter A1 (ABCA1), a major cholesterol transporter for apoA-I in macrophages, it could significantly diminish the effect of apoA-I on the sCD40L-stimulated inflammatory response. CONCLUSION: ApoA-I suppresses CD40 proinflammatory signaling in macrophages by preventing TRAF-6 translocation to lipid rafts through ABCA1-dependent regulation of free cholesterol (FC) efflux, which may present a novel mechanism of apoA-I-mediated inflammation inhibition in macrophages.
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英文
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