期刊论文

中文

中国动脉硬化杂志

1007-3949

2013

21

4

311-314

湖南省卫生厅科技项目（132007101） 博士启动基金（B2011XQD44）资助

本校为第一机构

目的 观察亲环素A对氧化型低密度脂蛋白诱导的RAW264.7细胞中核因子κB (NF-κB)表达的影响.方法 Western blot检测氧化型低密度脂蛋白处理RAW264.7细胞不同时间亲环素A和NF-κB蛋白的表达,siRNA沉降亲环素A后再用Western blot检测氧化型低密度脂蛋白对RAW264.7细胞中NF-κB蛋白表达的影响.结果 氧化型低密度脂蛋白能够上调RAW264.7细胞中亲环素A和NF-κB蛋白的表达,siRNA沉降亲环素A能降低细胞中NF-κB蛋白的表达.结论 亲环素A表达的改变能影响氧化型低密度脂蛋白诱导的RAW264.7细胞中NF-κB的蛋白表达.

Aim To observe the effect of Cyclophilin A on the expression of nuclear factor-KB （NF-KB） protein in RAW264. 7 cells induced by oxidized low density lipoprotein （ox-LDL）. Methods Western blot was applied to detect the expression of Cyclophilin A and NF-κB protein in RAW264. 7 cells induced by ox-LDL. Western blot was ap- plied to detect the expression of Cyclophilin A and NF-κB protein in Cyclophilin A siRNA RAW264. 7 ceils induced by ox- LDL. Results The expression of Cyelophilin A and NF-KB protein in RAW264.7 ceils was up-regulated by ...