作者机构：
[Fu, Lichun; Zhen, Deshuai; Yang, Jing; Xiong, Lihao; Luo, Chunhua] Hengyang Market Supervis Inspect & Testing Ctr, Hengyang 421001, Peoples R China.;[Zhen, Deshuai; Xiong, Lihao; Yang, Fei] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Gao, Linxiao; Zhen, Deshuai; Luo, Xiaohu] Qiannan Normal Univ Nationalities, Engn Res Ctr Loss Efficacy & Anticorros Mat Guizho, Sch Chem & Chem Engn, Duyun 558000, Peoples R China.;[Zhou, Huang] Huaihua Univ, Coll Chem & Mat Engn, Huaihua 418000, Peoples R China.;[Yang, Lixia] Nanchang Hangkong Univ, Key Lab Jiangxi Prov Persistent Pollutants Control, Nanchang 330063, Peoples R China.
通讯机构：
[Gao, LX] Q;[Zhen, DS ] H;Hengyang Market Supervis Inspect & Testing Ctr, Hengyang 421001, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;Qiannan Normal Univ Nationalities, Engn Res Ctr Loss Efficacy & Anticorros Mat Guizho, Sch Chem & Chem Engn, Duyun 558000, Peoples R China.
关键词：
Yb@TiO2;Perfluorooctane Sulfonate;Tetrabromobisphenol A;Contaminants;SELDI-TOF MS
摘要：
Ytterbium modified TiO2 nanoparticles (Yb@TiO2), synthesized by a sol-gel process, were employed as an efficient adsorbent and matrix for the analysis of Perfluorooctane sulfonate (PFOS) and Tetrabromobisphenol A (TBBPA) via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The detection limits for PFOS and TBBPA are achievement with 0.01 pg center dot mL-1. The results of the analysis of camellia oil samples show good recovery (80.3%-86.8%) with a low detection limit for TBBPA.
摘要：
Halloysite nanotubes (HNTs) are nanomaterials (NMs) derived from natural clays and have been considered as biocompatible NMs for biomedical uses. However, the cardiovascular toxicity of HNTs has not been thoroughly investigated. In this study, we compared the cardiotoxicity of HNTs and multi-walled carbon nanotubes (MWCNTs), focusing on the changes in Kruppel-like factor (KLF)-mediated signaling pathways. Mice were intravenously injected with 50µg NMs, once a day, for 5 days, and then mouse hearts were removed for experiments. While HNTs or MWCNTs did not induce obvious pathological changes, RNA-sequencing data suggested the alterations of KLF gene expression. We further confirmed an increase of Klf15 positive cells, accompanied by changes in Klf15-related gene ontology (GO) terms. We noticed that most of the changed GO terms are related with the regulation of gene expression, and we confirmed that the NMs increased myoneurin (Mynn) but decreased snail family transcriptional repressor 1 (Snai1), two transcription factors (TFs) related with Klf15. Besides, the changed GO terms also include metal ion binding and positive regulation of glucose import, and we verified an increase of phosphoenolpyruvate carboxykinase 1 (Pck1) and insulin receptor (Insr). However, HNTs and MWCNTs only showed minimal impact on cell death signaling pathways, and no increase in apoptotic sites was observed after NM treatment. We concluded that intravenous administration of HNTs and MWCNTs activated a protective TF, namely Klf15 in mouse aortas, to alter gene expression and signaling pathways related with metal ion binding and glucose import.
摘要：
Multiple pesticides are often used in combination for plant protection and public health. Therefore, it is important to analyze the physiological changes induced by multiple pesticides exposure. The objective of this study was to investigate the combined toxicity of the widely-used organophosphorus and pyrethroid pesticides diazinon, dimethoate, and cypermethrin. Male Wistar rats were administrated by gavage once daily with the three pesticides individual or in combination for consecutive 28 days. The metabolic components of serum and urine samples were detected by using 1H nuclear magnetic resonance (NMR)-based metabolomics method. Histopathological examination of liver and kidneys and serum biochemical determination were also carried out. The results showed that after the 28-day subacute exposure, serum glutamic transaminase and albumin were significantly increased and blood urea nitrogen was significantly decreased in the rats exposed to the mixture of the pesticides compared with the control rats, suggesting that the co-exposure impaired liver and kidney function. Metabolomics analysis indicated that the indicators 14 metabolites were statistically significant altered in the rats after the exposure of the pesticides. The increase in 3-hydroxybutyric acid in urine or decrease of lactate and N-acetyl-L-cysteine in serum could be a potentially sensitive biomarker of the subchronic combined effects of the three insecticides. The reduction level of 2-oxoglutarate and creatinine in urine may be indicative of dysfunction of liver and kidneys. In summary, the exposure of rats to pesticides diazinon, dimethoate, and cypermethrin could cause disorder of lipid and amino acid metabolism, induction of oxidative stress, and dysfunction of liver and kidneys, which contributes to the understanding of combined toxic effects of the pesticides revealed by using the metabolomics analysis of the urine and serum profiles.
摘要：
Given that intricate toxicological profiles exist among different antibiotics and pose serious threats to the environment and human health, synchronous analysis of multiple residues becomes crucial. Sensor arrays show potential to achieve the above purpose, but it is challenging to develop easy-to-use and high-sensitivity tools because the state-of-the-art arrays often require more than one recognition unit and are monosignal dependent. Here we exquisitely designed a fluorescent nanoprobe (2-aminoterephthalic acid-anchored CdTe quantum dots with Eu3+ coordination, CdTe-ATPA-Eu3+) featuring triple emissions at the same excitation as the only element to fabricate a luminescent sensor array with ratiometric calculations for identifying multiple antibiotics. By taking tetracycline, chlortetracycline, doxycycline, oxytetracycline, penicillin G, and sulfamethoxazole as models, the six species exhibited distinguishable motivation or/and quenching impacts on the three emissions of CdTe-ATPA-Eu3+, which were employed as indicators to perform the ratiometric logical operation and further combined with pattern recognition analysis for multitarget determination. Evidently, such a design exhibits two advances: (1) with the triple-emission probe as the sole receptor requiring neither internal nor external adjustments, the fabricated array acts as an extremely facile tool for multianalyte detection; (2) the ratiometric calculations offer excellent sensitivity and reliability for high-performance determination. Consequently, accurate identification and quantification of individual antibiotics and their combinations at various levels were verified in both laboratory and practical matrices. Our work provides a new tool for simultaneously detecting multiple antibiotics, and it will inspire the development of advanced sensor arrays for multitarget analysis.
通讯机构：
[Yang, SY ] U;Univ South China, Coll Publ Hlth, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Hunan, Peoples R China.
关键词：
Cobalt -nitrogen co -doped carbon dots;Colorimetric;Fluorometric;Dual -mode;Gallic acid
摘要：
Gallic acid (GA), widely used in food and medicine production industries. Herein, a kind of newly synthesized cobalt-nitrogen co-doped carbon dots (Co,N-CDs) with dual functional properties of oxidase-like activity and photoluminescence property could oxidize the 3,3 ',5,5 '-tetramethylbenzidine (TMB) to oxTMB with a new ab-sorption peak at 653 nm. In addition, it was found that oxTMB performed a fluorescence-quenching effect on Co, N-CDs at 440 nm attributed to FRET (Fluorescence resonance energy transfer). Reductive GA could reduce oxTMB to TMB, weaken the blue color, and restore the fluorescence of Co,N-CDs. Thus, a colorimetric and fluorometric method for dual-mode detection of GA was established. The synthesis of the dual-signal probe was completed with a single and environmental precursor in just one step. Simultaneously, the dual-mode detection reduced detection limits, broadened the linear range and improved anti-interference ability. This method exhibited a satisfactory application prospect in the actual detection of GA in tea beverages.
作者机构：
[Wang, Jingyu; Bai, Qinqin; Liang, H; Zheng, Yi; Zhao, Fengxia; Liang, Hao; Yan, Hangli; Wu, Linghao; Niu, Xiangheng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.;[Hu, Hongmei] Hengyang Ctr Dis Control & Prevent, Hengyang 421001, Peoples R China.
通讯机构：
[Liang, H ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.
摘要：
The detection of foodborne pathogens is crucial for food hygiene regulation and disease diagnosis. Colorimetry has become one of the main analytical methods in studying foodborne pathogens due to its advantages of visualization, low cost, simple operation, and no complex instrument. However, the low sensitivity limits its applications in early identification and on-site detection for trace analytes. In order to overcome such a limitation, herein we propose a joint strategy featuring dual signal amplification based on the hybridization chain reaction (HCR) and DNA-enhanced peroxidase-like activity of gold nanoparticles (AuNPs) for the sensitive visual detection of Escherichia coli. Target bacteria bound specifically to the aptamer domain in the capture hairpin probe, exposing the trigger domain for HCR and forming the extended double-stranded DNA (dsDNA) structures. The peroxidase-like catalytic capacity of AuNPs can be enhanced significantly by dsDNAs with the sticky ends of dsDNAs being adsorbed on AuNPs and the rigidity of dsDNAs causing the spatial regulation of AuNP concentration. The intensity of the enhancement was linearly related to the number of target bacteria. With the above strategy, the detection limit of our colorimetric method for Escherichia coli was down to 28 CFU mL(-1) within a short analytical time (50 min). This study provides a new perspective for the sensitive and visual detection of early bacterial contamination in foods.
作者机构：
[Min, Junxia; Yu, Yingying; Wang, Fudi; Liu, Yutong; Zhou, Jiahui; Yue, Wuyang; Su, Yunxing; Yang, Sisi; Li, Xiaopeng; Min, JX; Sun, Shumin] Zhejiang Univ, Affiliated Hosp 1, Affiliated Hosp 2, Inst Translat Med,Sch Med, Hangzhou 310058, Peoples R China.;[Yu, Yingying; Wang, Fudi; Lin, Zhiting] Univ South China, Affiliated Hosp 1, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Shah, YM; Shah, Yatrik M.; Das, Nupur K.] Univ Michigan, Div Gastroenterol, Internal Med, Ann Arbor, MI 48109 USA.;[Shah, YM; Shah, Yatrik M.; Das, Nupur K.] Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA.;[Wu, Qian] Zhejiang Univ, Int Inst Med, Sch Med, Yiwu, Zhejiang, Peoples R China.
通讯机构：
[Wang, FD ; Min, JX] Z;[Shah, YM ] U;Zhejiang Univ, Affiliated Hosp 1, Affiliated Hosp 2, Inst Translat Med,Sch Med, Hangzhou 310058, Peoples R China.;Univ South China, Affiliated Hosp 1, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Univ Michigan, Div Gastroenterol, Internal Med, Ann Arbor, MI 48109 USA.
关键词：
FG-4592;HIF;anemia of inflammation;chemotherapy-induced anemia;hypoxia;iron-refractory iron deficiency anemia
摘要：
In clinics, hepcidin levels are elevated in various anemia-related conditions, particularly in iron-refractory anemia and in high inflammatory states that suppress iron absorption, which remains an urgent unmet medical need. To identify effective treatment options for various types of iron-refractory anemia, the potential effect of hypoxia and pharmacologically-mimetic drug FG-4592 (Roxadustat) are evaluated, a hypoxia-inducible factor (HIF)-prolyl hydroxylase (PHD) inhibitor, on mouse models of iron-refractory iron-deficiency anemia (IRIDA), anemia of inflammation and 5-fluorouracil-induced chemotherapy-related anemia. The potent protective effects of both hypoxia and FG-4592 on IRIDA as well as other 2 tested mouse cohorts are found. Mechanistically, it is demonstrated that hypoxia or FG-4592 could stabilize duodenal Hif2 alpha, leading to the activation of Fpn transcription regardless of hepcidin levels, which in turn results in increased intestinal iron absorption and the amelioration of hepcidin-activated anemias. Moreover, duodenal Hif2 alpha overexpression fully rescues phenotypes of Tmprss6 knockout mice, and Hif2 alpha knockout in the gut significantly delays the recovery from 5-fluorouracil-induced anemia, which can not be rescued by FG-4592 treatment. Taken together, the findings of this study provide compelling evidence that targeting intestinal hypoxia-related pathways can serve as a potential therapeutic strategy for treating a broad spectrum of anemia, especially iron refractory anemia. In this article, it is demonstrated that targeting the duodenal Hif2 alpha-Fpn axis as a novel strategy to improve refractory hepcidin-activated anemias, including iron-refractory iron-deficiency anemia (IRIDA), inflammatory anemia and chemotherapy-induced anemia, in mice, which provides compelling evidence for further clinical translation.image
摘要：
Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) persistently kills nearly 1.5 million lives per year in the world, whereas the only licensed TB vaccine BCG exhibits unsatisfactory efficacy in adults. Taking BCG as a vehicle to express Mtb antigens is a promising way to enhance its efficacy against Mtb infection. In this study, the immune efficacy of recombination BCG (rBCG-ECD003) expressing specific antigens ESAT-6, CFP-10, and nDnaK was evaluated at different time points after immunizing BALB/c mice. The results revealed that rBCG-ECD003 induced multiple Th1 cytokine secretion including IFN-γ, TNF-α, IL-2, and IL-12 when compared to the parental BCG. Under the action of PPD or ECD003, rBCG-ECD003 immunization resulted in a significant increase in the proportion of IL-2(+) and IFN-γ(+)IL-2(+) CD4(+)T cells. Importantly, rBCG-ECD003 induced a stronger long-term humoral immune response without compromising the safety of the parental BCG vaccine. By means of the protective efficacy assay in vitro, rBCG-ECD003 showed a greater capacity to inhibit Mtb growth in the long term. Collectively, these features of rBCG-ECD003 indicate long-term protection and the promising effect of controlling Mtb infection.
摘要：
Uranium, regarded as an important element in nuclear fission energy schemes, is harmful to public health owing to its high toxicity and radioactivity. Herein, a novel fluorescent probe (SC4A@Tb3+) was developed using a one-step liquid-phase method with 4-sulfocalix[4]arene (SC4A) and rare earth-metal ions (Tb3+) as precursors to realize the accurate monitoring of uranyl ion (UO22+) detection. Based on the dynamic quenching mechanism, the SC4A@Tb3+ fluorescence quenching sensor for UO22+ detection was in the linear range of 0-0.36 mu M, with a detection limit of 20 mu g center dot L-1, and successfully quantified UO22+ in water and human serum samples. These results suggest that SC4A@Tb3+ is a potential sensing material for UO22+ detection.
摘要：
How to fabricate a multifunctional coating with controllable ultralow refractive index (RI), high antifogging and anticorrosion performances by a facile method is still one of the great challenges. In this work, a simple and time-saving approach involving a microwave-assisted sol-sel process was developed to fabricate a novel core-shell structured silica nanoparticle sol by co-condensation of perfluorodecyltriethoxysilane (F-DTOS) and tetraethoxysilane (TEOS) and self-condensation of F-DTOS. Results suggest that both the co-condensations and self-condensations not only give a layer of low surface energy -C10H4F17 groups on the silica particles but also enlarge the hybrid silica particle size, providing more and larger voids to form the high porosity. After spinning the silica nanoparticles on the substrate, the obtained optical coating exhibits the controllable ultralow refractive index (RI) and outstanding transparency. The minimum RI and maximum transmittance of our developed coating is up to similar to 1.04 and similar to 100 %, respectively. Moreover, because of the high porosity and the presence of low surface energy -C10H4F17 groups in the coating, the coating has the high hydrophobicity (water contact angle similar to 108.8 degrees), which not only makes it excellent antifogging performance but also affords it high-efficient anticorrosion property. After immersion in a 3.5 wt% NaCl solution for 7 days, the R-ct value of the coating could be higher than 7.3 x 10(4) Omega cm(2). It is believed that our developed method used to fabricate such multifunctional coating does not need any costly equipments, complex steps and time-consuming, making it well suited for industrial application.
摘要：
Tumor cell metastasis is the key cause of death in patients with nasopharyngeal carcinoma (NPC). MiR-2110 was cloned and identified in Epstein-Barr virus (EBV)-positive NPC, but its role is unclear in NPC. In this study, we investigated the effect of miR-2110 on NPC metastasis and its related molecular basis. In addition, we also explored whether miR-2110 can be regulated by cinobufotalin (CB) and participate in the inhibition of CB on NPC metastasis. Bioinformatics, RT-PCR, and in situ hybridization were used to observe the expression of miR-2110 in NPC tissues and cells. Scratch, Boyden, and tail vein metastasis model of nude mouse were used to detect the effect of miR-2110 on NPC metastasis. Western blot, Co-IP, luciferase activity, colocalization of micro confocal and ubiquitination assays were used to identify the molecular mechanism of miR-2110 affecting NPC metastasis. Finally, miR-2110 induced by CB participates in CB-stimulated inhibition of NPC metastasis was explored. The data showed that increased miR-2110 significantly suppresses NPC cell migration, invasion, and metastasis. Suppressing miR-2110 markedly restored NPC cell migration and invasion. Mechanistically, miR-2110 directly targeted FGFR1 and reduced its protein expression. Decreased FGFR1 attenuated its recruitment of NEDD4, which downregulated NEDD4-induced phosphatase and tensin homolog (PTEN) ubiquitination and degradation and further increased PTEN protein stability, thereby inactivating PI3K/AKT-stimulated epithelial-mesenchymal transition signaling and ultimately suppressing NPC metastasis. Interestingly, CB, a potential new inhibitory drug for NPC metastasis, significantly induced miR-2110 expression by suppressing PI3K/AKT/c-Jun-mediated transcription inhibition. Suppression of miR-2110 significantly restored cell migration and invasion in CB-treated NPC cells. Finally, a clinical sample assay indicated that reduced miR-2110 was negatively correlated with NPC lymph node metastasis and positively related to NPC patient survival prognosis. In summary, miR-2110 is a metastatic suppressor involving in CB-induced suppression of NPC metastasis.
摘要：
An in vivo model is necessary for toxicology. This review analyzed the uses of zebrafish (Danio rerio) in toxicology based on bibliometrics. Totally 56,816 publications about zebrafish from 2002 to 2023 were found in Web of Science Core Collection, with Toxicology as the top 6 among all disciplines. Accordingly, the bibliometric map reveals that "toxicity" has become a hot keyword. It further reveals that the most common exposure types include acute, chronic, and combined exposure. The toxicological effects include behavioral, intestinal, cardiovascular, hepatic, endocrine toxicity, neurotoxicity, immunotoxicity, genotoxicity, and reproductive and transgenerational toxicity. The mechanisms include oxidative stress, inflammation, autophagy, and dysbiosis of gut microbiota. The toxicants commonly evaluated by using zebrafish model include nanomaterials, arsenic, metals, bisphenol, and dioxin. Overall, zebrafish provide a unique and well-accepted model to investigate the toxicological effects and mechanisms. We also discussed the possible ways to address some of the limitations of zebrafish model, such as the combination of human organoids to avoid species differences.
摘要：
Deinococcus radiodurans (DR) exhibits strong resistance to ionizing radiation. In this study, by constructing a radiation-resistant genetically engineered strain overexpressing the Cs gene, the tolerance of the bacterium to aluminum ions was enhanced, thereby achieving the goal of microbial sustainable remediation of uranium-contaminated environments. Methods: 1. Extraction of the recombinant plasmid pRADK-Cs, transformation into DR, and verification. 2. Investigation of factors such as time and initial uranium concentration on the efficiency of uranium accumulation by the recombinant strain, characterized by changes in functional groups and surface morphology before and after accumulation using techniques such as scanning electron microscope. Conclusions: The recombinant strain Deino-Cs can reduce the inhibitory effect of aluminum ions on uranium accumulation capability, and it exhibits a higher uranium accumulation rate compared to the wild-type strain.
期刊：
Journal of Molecular Structure,2024年1298:137042 ISSN：0022-2860
通讯作者：
Wang, T;Mao, Longfei;Li, SQ
作者机构：
[Guo, Yajie; Wang, Tong; Wang, T; Hou, Jingyu] Sun Yat Sen Univ, Affiliated Hosp 8, Dept Emergency, Shenzhen 518033, Peoples R China.;[Wu, Hao] Sun Yat sen Univ, Sun Yat Sen Mem Hosp, Dept Emergency, Guangzhou 510120, Peoples R China.;[Chen, Ying; Liu, Guangnan] Univ South China, Sch Publ Hlth, Hengyang 421000, Peoples R China.;[Chen, Ying; Liu, Guangnan] Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Peoples R China.;[Wang, Dan] Southern Med Univ, Sch Publ Hlth, Guangzhou 510515, Peoples R China.
通讯机构：
[Li, SQ ; Mao, LF] H;[Wang, T ] S;Sun Yat Sen Univ, Affiliated Hosp 8, Dept Emergency, Shenzhen 518033, Peoples R China.;Henan Univ Sci & Technol, Coll Basic Med & Forens Med, Luoyang 471003, Peoples R China.
关键词：
1,2,3-Triazole;Cabotegravir;Anti-cancer
摘要：
A series of 28 novel cabotegravir derivatives, incorporating the 1,2,3-triazole moiety, were designed and synthesized. The synthesized compounds were evaluated for their potential anticancer activity against four different human cancer cell lines: HuH-7 (hepatocellular), MCF-7 (breast), SKOV3 (ovarian), and HCT-116 (colon). Initial biological assessments revealed that several compounds exhibited potent anti-proliferative activity against these cancer cell lines. Notably, compounds KJ9 and KJ23 demonstrated the most pronounced effects, with IC50 values of 6.59 and 7.83 mu M in HuH-7 cells, 27.24 and 8.59 mu M in MCF-7 cells, 4.46 and 6.30 mu M in SKOV3 cells, 23.90 and 17.00 mu M in HCT-116 cells, respectively. Further investigations demonstrated that compound KJ9 and KJ23 induced cell apoptosis, elevated reactive oxygen species (ROS) levels, and ultimately led to cell death. Additionally, western blot analysis revealed altered expressions of proteins involved in autophagy and DNA damage following treatment with compound KJ9 and KJ23. Molecular structure analysis found that the novel compounds tend towards a planar conformation which contained multiple rigid planar structures. This planar structure makes it possible for the compounds to intercalate into DNA and causing DNA damage. These findings suggested that cabotegravir-1,2,3-triazole derivatives possess potential antitumor abilities and warrant further investigations for the development of novel anticancer drugs.
作者：
Kim, Han-Sol;Bui, Quynh Thi Nhu;Shin, Jeongmin;Wang, Hui*;Ki, Jang-Seu
期刊：
Journal of Applied Phycology,2024年:1-15 ISSN：0921-8971
通讯作者：
Wang, Hui;Ki, JS;Wang, H
作者机构：
[Wang, Hui; Kim, Han-Sol; Shin, Jeongmin; Ki, Jang-Seu; Bui, Quynh Thi Nhu; Ki, JS] Sangmyung Univ, Dept Biotechnol, Seoul 03016, South Korea.;[Wang, Hui] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, H; Ki, JS ] S;[Wang, H ] U;Sangmyung Univ, Dept Biotechnol, Seoul 03016, South Korea.;Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
关键词：
STX biosynthesis gene (sxt);Promoter;Untranslated region (UTR);Transcriptional regulation;Alexandrium catenella
摘要：
The marine dinoflagellate Alexandrium catenella (Group I) produces saxitoxins (STXs) in marine environments, causing paralytic shellfish poisoning (PSP). The toxins are synthesized by STXs biosynthesis genes (sxt); of them, sxtA and sxtG are considered the most essential. The promoter region and 3'-untranslated region (3'-UTR) are known to regulate gene transcription; however, they have not been sufficiently elucidated in the sxt genes. Herein, we determined and characterized the genomic DNA sequence of the proximal regions of sxtA and sxtG in A. catenella. The promoter regions of sxtA and sxtG in A. catenella were found to contained distinct cis-regulatory elements (CREs), CpG islands, and TTTT-motif (instead of TATA-box), showing a typical eukaryotic promoter. Especially, a number of light-responsive CREs (G-box, SORLIP1AT/2AT, and Sp1) were found, and the gene expression level showed a significant relationship with photosynthesis. MiSeq sequencing of the long PCR amplicons revealed that sxtA was encoded as a single exonic structure that is discrete (> 5 kbp) from other core sxt. In addition, we reported for the first time two types of 3'-UTR in sxtG mRNA, and their lengths and sequences varied among strains and species. Such results suggest that sxt may be regulated by post-transcription. Our results provide insights into the toxin dynamics of toxic dinoflagellates from a molecular perspective.
作者机构：
[Wu, Xiaotian; Song, Zijun; Sun, Shumin; Su, Yunxing; Cheng, Xihao; Yu, Yingying; Yu, Chao; Min, Junxia] The Second Affiliated Hospital, The First Affiliated Hospital, School of Public Health, Institute of Translational Medicine, Cancer Center, Zhejiang University School of Medicine, Hangzhou, China;[Wang, Tianyi] The First Affiliated Hospital, Basic Medical Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China;Division of Nephrology, Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany;Division of Nephrology, Department of Medicine, Albert Einstein College of Medicine, Bronx, USA;[Chen, En; Chen, Wenteng; Yu, Yongping] College of Pharmaceutical Science, Zhejiang University, Hangzhou, China
通讯机构：
[Junxia Min; Fudi Wang] T;The Second Affiliated Hospital, The First Affiliated Hospital, School of Public Health, Institute of Translational Medicine, Cancer Center, Zhejiang University School of Medicine, Hangzhou, China<&wdkj&>The First Affiliated Hospital, Basic Medical Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>The Second Affiliated Hospital, The First Affiliated Hospital, School of Public Health, Institute of Translational Medicine, Cancer Center, Zhejiang University School of Medicine, Hangzhou, China
摘要：
Pyroptosis is a gasdermins-mediated programmed cell death that plays an essential role in immune regulation, and its role in autoimmune disease and cancer has been studied extensively. Increasing evidence shows that various microbial infections can lead to pyroptosis, associated with the occurrence and development of microbial infectious diseases. This study reviews the recent advances in pyroptosis in microbial infection, including bacterial, viral, and fungal infections. We also explore potential therapeutic strategies for treating microbial infection-related diseases by targeting pyroptosis.
摘要：
Four new ansamycin derivatives, named 1,19-epithio-geldanamycin A (1), 17-demethoxylherbimycin H (2), herbimycin M (3), and seco-geldanamycin B (4), together with eight known ansamycin analogues (5-12) were isolated from the solid fermentation of marine-derived actinomycete Streptomyces sp. ZYX-F-97. The structures of new compounds were elucidated by extensive spectroscopic analysis as well as nuclear magnetic resonance (NMR) and electronic circular dichroism (ECD) calculations. All the compounds were assayed for their antibacterial activity. Among them, compounds 4, 8, and 12 exhibited remarkable inhibition against Listeria monocytogenes with minimum inhibitory concentrations (MIC) values ranging from 8 mu g & sdot;mL- 1 to 64 mu g & sdot;mL- 1, and displayed moderate inhibition against methicillin-resistant Staphylococcus aureus (MRSA) with MIC value of 64 mu g & sdot;mL- 1. Compounds 4, 8, 9, and 12 showed moderate inhibition activities against both Staphylococcus aureus and Bacillus subtilis with MIC values ranging from 32 mu g & sdot;mL- 1 to 128 mu g & sdot;mL- 1.
摘要：
The discovery of enzyme-like catalytic characteristics in nanomaterials triggers the generation of nanozymes and their multifarious applications. As a class of artificial mimetic enzymes, nanozymes are widely recognized to have better stability and lower cost than natural bio-enzymes, but the lack of catalytic specificity hinders their wider use. To solve the problem, several potential strategies are explored, among which molecular imprinting attracts much attention because of its powerful capacity for creating specific binding cavities as biomimetic receptors. Attractively, introducing molecularly imprinted polymers (MIPs) onto nanozyme surfaces can make animpact on the latter's catalytic activity. As a result, in recent years, MIPs featuring universal fabrication, low cost, and good stability have been intensively integrated with nanozymes for biochemical detection. In this critical review, we first summarize the general fabrication of nanozyme@MIPs, followed by clarifying the potential effects of molecular imprinting on the catalytic performance of nanozymes in terms of selectivity and activity. Typical examples are emphatically discussed to highlight the latest progress of nanozyme@MIPs applied in catalytic analysis. In the end, personal viewpoints on the future directions of nanozyme@MIPs are presented, to provide a reference for studying the interactions between MIPs and nanozymes and attract more efforts to advance this promising area.
摘要：
The N6-methyladenosine (M6A) modification is the most common internal chemical modification of RNA molecules in eukaryotes. This modification can affect mRNA metabolism, regulate RNA transcription, nuclear export, splicing, degradation, and translation, and significantly impact various aspects of physiology and pathobiology. Radiotherapy is the most common method of tumor treatment. Different intrinsic cellular mechanisms affect the response of cells to ionizing radiation (IR) and the effectiveness of cancer radiotherapy. In this review, we summarize and discuss recent advances in understanding the roles and mechanisms of RNA M6A methylation in cellular responses to radiation-induced DNA damage and in determining the outcomes of cancer radiotherapy. Insights into RNA M6A methylation in radiation biology may facilitate the improvement of therapeutic strategies for cancer radiotherapy and radioprotection of normal tissues.
