期刊：
International Journal of Biological Macromolecules,2024年259(Pt 1):129104 ISSN：0141-8130
通讯作者：
Liu, Y
作者机构：
[Liu, Yu; Zhang, Shaoqi; Li, Le; Zhen, Deshuai; Yang, Aofeng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.;[Liu, Yu; Zhang, Shaoqi; Zhen, Deshuai] Hunan Univ, Sch Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Grimes, Craig A.] Flux Photon Corp, 5950 Shiloh Rd East, Alpharetta, GA 30005 USA.;[Liu, Yu] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Liu, Y ] H;Hunan Univ, Sch Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.
关键词：
Covalent organic framework;Fluorescent probe;In vivo imaging
摘要：
Simple and accurate in vivo monitoring of Fe(3+) is essential for gaining a better understanding of its role in physiological and pathological processes. A novel fluorescent probe was synthesized via in situ solid-state polymerization of 3,4-ethylenedioxythiophene (PEDOT) in the pore channels of a covalent organic framework (COF). The PEDOT@COF fluorescent probe exhibited an absolute quantum yield (QY) 3 times higher than COF. In the presence of Fe(3+) the PEDOT@COF 475nm fluorescence emission, 365nm excitation, is quenched within 180s. Fluorescence quenching is linear with Fe(3+) in the concentration range of 0-960μM, with a detection limit of 0.82μM. The fluorescence quenching mechanism was attributed to inner filter effect (IEF), photoinduced electron transfer (PET) and static quenching (SQE) between PEDOT@COF and Fe(3+). A paper strip-based detector was designed to facilitate practical applicability, and the PEDOT@COF probe successfully applied to fluorescence imaging of Fe(3+) levels in vivo. This work details a tool of great promise for enabling detailed investigations into the role of Fe(3+) in physiological and pathological diseases.
期刊：
Human & Experimental Toxicology,2024年43 ISSN：0960-3271
通讯作者：
Wang, M;Yang, Q
作者机构：
[Ou, Guifang; Liu, Xiaocheng; Qin, Xinru; Liu, Xu; Tang, Yonghong; Rang, Ouyan; Wang, Mu; Yang, Qing] Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Nucl Ind Hyg Sch, Clin Res Inst,Hengyang Med Sch, Hengyang, Peoples R China.;[Ou, Guifang; Liu, Xiaocheng; Qin, Xinru; Liu, Xu; Tang, Yonghong; Rang, Ouyan; Wang, Mu; Yang, Qing] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Basic Med,Nucl Ind Hyg Sch, Hengyang, Peoples R China.;[Qin, Xinru; Liu, Xu] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Li, Guojuan] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Endocrinol Dept, Hengyang, Peoples R China.;[Wang, Mu] Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Clin Res Inst, Hengyang Med Sch,Nucl Ind Hyg Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wang, M ; Yang, Q ] U;Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Clin Res Inst, Hengyang Med Sch,Nucl Ind Hyg Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Dept Basic Med,Nucl Ind Hyg Sch, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dongfeng Nan Rd 336, Hengyang, Peoples R China.
摘要：
Human & Experimental Toxicology, Volume 43, Issue , January-December 2024. <br/>Adipose tissue is the main organ that stores lipids and it plays important roles in metabolic balance in the body. We recently reported in Human and Experimental Toxicology that the combined exposure to BPA and fructose may interfere with energy metabolism of adipose tissue. However, it is still unclear whether the combined exposure to BPA and fructose has the possibility to induce lipid remodeling in adipose tissue. In the present study, we performed a widely targeted quantitative lipidomic analysis of the adipose tissue of rats after 6 months of BPA and fructose combined exposure. We totally determined 734 lipid molecules in the adipose tissue of rats. Principal component analysis (PCA) showed the group of the combined exposure to higher-dose (25 μg/kg every other day) BPA and fructose can be distinguished from the groups of control, higher-dose BPA exposure and fructose exposure clearly. Partial least squares-discriminant analysis (PLS-DA) and univariate statistical analysis displayed lipids of PC(18:0_ 20:3), TG(8:0_14:0_16:0), TG(12:0_14:0_16:1), TG(10:0_16:0_16:1), TG(12:0_ 14:0_18:1), TG(14:0_ 16:0_16:1), TG(14:0_14:1_16:1), TG(8:0_ 16:1_16:2), TG(14:1_16:1_ 16:1), TG(16:1_18:1_18:1), TG(16:0_16:1_20:4) and TG(15:0_18:1_ 24:1) may contributed the most to the discrimination. These findings indicated that combined exposure to BPA and fructose has the potential to cause lipid remodeling in adipose tissue.
摘要：
Microcystins are highly toxic cyanotoxins and have been produced worldwide with the global expansion of harmful cyanobacterial blooms (HABs), posing serious threats to human health and ecosystem safety. Yet little knowledge is available on the underlying process occurring in the aquatic environment with microcystins. Microplastics as vectors for pollutants has received growing attention and are widely found co-existing with microcystins. On the one hand, microplastics could react with microcystins by adsorption, altering their environmental behavior and ecological risks. On the other hand, particular attention should be given to microplastics due to their implications on the outbreak of HABs and the generation and release of microcystins. However, limited reviews have been undertaken to link the co-existing microcystins and microplastics in natural water. This study aims to provide a comprehensive understanding on the environmental relevance of microcystins and microplastics and their potential interactions, with particular emphasis on the adsorption, transport, sources, ecotoxicity and environmental transformation of microcystins affected by microplastics. In addition, current knowledge gaps and future research directions on the microcystins and microplastics are presented. Overall, this review will provide novel insights into the ecological risk of microcystins associated with microplastics in real water environment and lay foundation for the effective management of HABs and microplastic pollution.
通讯机构：
[Yang, SY; Li, L ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Hlth Inspect & Quarantine, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
摘要：
Mercury is a highly toxic element that is widely present in all types of environmental media and can accumulate in living organisms. Prolonged exposure to high levels of mercury can lead to brain damage and death, so the detection of mercury is of great importance. In this study, a cost-effective and easy-to-operate electrochemical sensing method was successfully developed based on an amino-functionalized titanium-based MXene (NH(2)-Ti(3)C(2)T(x)) for the rapid and selective detection of Hg(2+) that could have a coordination effect with the -NH(2) group of NH(2)-Ti(3)C(2)T(x) to promote the efficient accumulation of Hg(2+). In this strategy, the NH(2)-Ti(3)C(2)T(x) was first modified on glassy carbon electrodes (GCE) to fabricate the electrochemical sensor. Benefiting from the excellent electrical conductivity, abundant active sites, and strong adsorption capacity performance of the NH(2)-Ti(3)C(2)T(x), the NH(2)-Ti(3)C(2)T(x) modified GCE (NH(2)-Ti(3)C(2)T(x)/GCE) exhibited satisfactory selectivity and enhanced square wave anodic stripping voltammetry (SWASV) measurement for the rapid detection of trace amounts of Hg(2+) in aqueous solutions. The electrochemical sensor was found to be capable of detecting Hg(2+) with a low detection limit of 8.27 nmol L(-1) and a linear range of 0.5 μmol L(-1) to 50 μmol L(-1). The response time of the electrochemical sensing method was 308 s. In addition, the electrochemical sensing method has good selectivity, repeatability and stability, and multiple heavy metal ions have no effect on its detection, with repeatability and stability RSDs of 1.68% and 1.43%, respectively. Furthermore, the analysis of practical water samples demonstrated that the developed method was highly practical for the actual determination of Hg(2+) with recoveries in the range of 99.22-101.90%.
作者机构：
[Zhang, Shaoqi; Deng, Zhongliang; He, Jun; He, J; Zhen, Deshuai] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.;[Fang, Jing; Zhang, Shaoqi; Deng, Zhongliang; Zhen, Deshuai; Yang, Aofeng; Ma, Qian] Univ South China, Coll Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.;[Cai, Qingyun; Zhen, Deshuai] Hunan Univ, Sch Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.
通讯机构：
[He, J ] U;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.
关键词：
CRISPR/Cas9;Rolling circle amplification;S. aureus;Silver chain
摘要：
With the rising emphasis on food safety, technology to rapidly identify Staphylococcus aureus (S. aureus) is of great significance. Herein, we developed a novel electrochemical biosensor based on the CRISPR/Cas9 system and rolling circle amplification (RCA)-assisted "silver chain"-linked gold interdigital electrodes (Au-IDE). This sensor utilizes RCA to create DNA long chains that span the Au-IDE, and CRISPR/Cas9 as a recognition component to recognize capture/target dsDNA. Additionally, we used silver staining technology to improve detection sensitivity. Then, we detected S. aureus through impedance changes that occurred when the silver chain between the Au-IDE was connected or broke, with a limit of detection (LOD) of 7CFU/mL and a detection time of 1.5h. Lastly, we successfully employed this sensor to detect S. aureus in real food samples, making it a promising tool for food monitoring.
作者机构：
[Diao, Qiaoqiao; Bu, Zhijian; Tian, Qingzhen; Liu, Jinjin; Tang, Zheng; Liu, Huiqing; Niu, Xiangheng; Li, Shu; Chen, Xinyu] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Niu, Xiangheng] Hunan Ecol & Environm Monitoring Ctr, State Environm Protect Key Lab Monitoring Heavy Me, Changsha 410019, Peoples R China.;[Niu, Xiangheng] Qingdao Univ Sci & Technol, Shandong Key Lab Biochem Anal, Qingdao 266042, Peoples R China.;[Niu, Xiangheng] Minjiang Univ, Fujian Key Lab Funct Marine Sensing Mat, Fuzhou 350108, Peoples R China.
通讯机构：
[Niu, XH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Hunan Ecol & Environm Monitoring Ctr, State Environm Protect Key Lab Monitoring Heavy Me, Changsha 410019, Peoples R China.;Qingdao Univ Sci & Technol, Shandong Key Lab Biochem Anal, Qingdao 266042, Peoples R China.;Minjiang Univ, Fujian Key Lab Funct Marine Sensing Mat, Fuzhou 350108, Peoples R China.
摘要：
The increasing environmental pollution and its threats to human health require efficient monitoring and remediation techniques. Enzymes play a significant role in environmental monitoring and remediation, but the vulnerable performance of natural enzymes greatly limits their wider use in complex matrices. As an alternative to natural enzymes, materials with enzyme-like catalytic characteristics have found many environmental applications. With the catalytic signal amplification feature, various nanozyme materials are explored to detect contaminants. Meanwhile, the robust catalytic performance endows nanozymes with potential use in pollutant removal. To highlight the exciting and blooming area, here we made a critical review on nanozyme-involved detection and degradation of environmental pollutants, and the research progress achieved in the last five years was emphatically concluded. First, the use of nanozymes in detecting various contaminants (toxic ions, phenolic pollutants, antibiotics, and pesticides) was discussed according to different sensing principles. Second, the strategies established with the participation of nanozymes for pollutant degradation were summarized. In the end, the opportunities given by nanozyme catalysis were presented for environmental control, and current challenges were discussed to better guide the field's development. Our review provides the up-to-date means and views for monitoring and degrading pollutants based on nanozyme catalysis. It is also anticipated to draw more attention and effort to further advance this promising area. Here we made a critical review on nanozyme-involved detection and degradation of environmental pollutants, and the research progress achieved in the last five years was emphatically concluded.
摘要：
Microbial degradation is a cost-effective and environmentally friendly method for removing microcystin-LR (MC-LR). However, the application of free bacteria has limitations due to low operational stability and difficulties in recovery. In a previous study, our group successfully isolated a highly efficient MC-LR-degrading bacterium, Sphingopyxis sp. YF1, from Taihu. To enhance its practical potential in addressing MC-LR-contaminated water pollution, a novel biological material named polyacrylonitrile-based carbon fiber @Sphingopyxis sp. YF1 (PAN-CF@YF1) was synthesized. The immobilization conditions of strain Sphingopyxis sp. YF1 on PAN-CF surfaces were optimized using Box–Behnken design and response surface methodology (RSM), which turned out to be an optimal pH of 7.6 for the culture medium, a ratio of 0.038 g of supporting materials per 100 mL of culture media, and an incubation time of 53.4 h. The resultant PAN-CF@YF1 showed a great degradation effect both for low and high concentrations of MC-LR and exhibited satisfactory cyclic stability (85.75% after six cycles). Moreover, the application of PAN-CF@YF1 in the bioreactors demonstrated effective and sustainable MC-LR removal, with a removal efficiency of 78.83% after three consecutive treatments. Therefore, PAN-CF@YF1 with high degradation activity, environmental compatibility, straightforward preparation, and recyclability shows significant application potential for the bioremediation of MC-LR-contaminated water bodies.
作者机构：
[Tan, Yan; Liu, Jinquan; Shi, Jiao; Li, Le; Wang, Yating; Zhen, Deshuai; Liu, JQ; Zhou, Xiayu] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Hlth Inspect & Quarantine, Hengyang 421001, Hunan, Peoples R China.;[Tan, Yan; Liu, Jinquan; Shi, Jiao; Li, Le; Wang, Yating; Zhen, Deshuai; Liu, JQ; Zhou, Xiayu] Univ South China, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.;[Tan, Yan; Liu, Jinquan; Shi, Jiao; Li, Le; Wang, Yating; Zhen, Deshuai; Liu, JQ; Zhou, Xiayu] Univ South China, Key Lab Hlth Hazard Factors Inspect & Quarantine, Hengyang 421001, Hunan, Peoples R China.;[Dai, Zhongran] Univ South China, Hunan Prov Key Lab Green Dev Technol Extremely Low, Hengyang 421001, Peoples R China.
通讯机构：
[Liu, JQ; Li, L ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Hlth Inspect & Quarantine, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.;Univ South China, Key Lab Hlth Hazard Factors Inspect & Quarantine, Hengyang 421001, Hunan, Peoples R China.
摘要：
In this study, we developed a simple and sensitive colorimetric sensing method for the detection of UO(2)(2+), which was built to release MB from the molybdenum disulfide with a phosphate group (MoS(2)-PO(4)) gated mesoporous silica nanoparticles functionalized phosphate group (MSN-PO(4)) with UO(2)(2+) chelating. In the presence of UO(2)(2+), MoS(2)-PO(4) can be effectively adsorbed onto the surface of MSN-PO(4) based on the coordination chemistry for strong affinity between the P-O bond and UO(2)(2+). The adsorbed MoS(2)-PO(4) was then utilized as an ideal gate material to control the release of signal molecules (MB) entrapped within the pores of MSN-PO(4), resulting in a detectable decrease in the absorption peak at 663 nm. This colorimetric sensing demonstrated the advantages of simplicity and easy manipulation and exhibited a linear response to the concentration of UO(2)(2+) within the range of 0.02-0.2 μM. The detection limit of UO(2)(2+) was determined to be 0.85 nM, which was lower than the limit (130 nmol L(-1)) set by the US Environmental Protection Agency. Furthermore, the proposed colorimetric sensing method has been utilized to determine UO(2)(2+) in samples of Xiangjiang River and tap water, and a high recovery rate was achieved. This method shows promising potential in preventing and controlling environmental pollution.
期刊：
JOURNAL OF INFECTIOUS DISEASES,2024年 ISSN：0022-1899
通讯作者：
Qu, XW
作者机构：
[Zheng, Xingyu; Liu, Fen; Qu, Xiaowang; Wu, Chanfeng; Lu, Rui; Xie, Tianyi; Liu, Wenpei] Univ South China, Coll Basic Med, Hengyang Med Sch, Hengyang, Peoples R China.;[Zheng, Xingyu; Chen, Jun; Wang, You; Liu, Fen; Tang, Jinyong; Pan, Dong; Tang, Yinggen; Wu, Chanfeng; Teng, Shishan; Peng, Liting; Lu, Rui; He, Rongzhang; Hu, Yabin; Rong, Xiaohan; Xie, Tianyi] Univ South China, Peoples Hosp Chenzhou 1, Translat Med Inst, Hengyang Med Sch, Chenzhou, Peoples R China.;[Wang, You] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Qu, Xiaowang] Univ South China, Coll Basic Med, Hengyang Med Sch, 28 West Changsheng Rd, Hengyang 421001, Peoples R China.
通讯机构：
[Qu, XW ] U;Univ South China, Coll Basic Med, Hengyang Med Sch, 28 West Changsheng Rd, Hengyang 421001, Peoples R China.
关键词：
COVID-19;Circulating T-Follicular Helper Cell;Neutralizing Antibody;Regulatory T cell
摘要：
Regulatory T (Treg) cells are involved in the antiviral immune response in patients with coronavirus disease 2019 (COVID-19); however, whether Treg cells are involved in the neutralizing antibody (nAb) response remains unclear. Here, we found that individuals who recovered from mild but not severe COVID-19 had significantly greater frequencies of Treg cells and lower frequencies of CXCR3+ circulating T follicular helper (cTfh) cells than healthy controls. Furthermore, the frequencies of Treg and CXCR3+ cTfh cells were negatively and positively correlated with the nAb responses, respectively, and Treg cells was inversely associated with CXCR3+ cTfh cells in individuals who recovered from mild COVID-19 but not in those with severe disease. Mechanistically, Treg cells inhibited memory B-cell differentiation and antibody production by limiting the activation and proliferation of cTfh cells, especially CXCR3+ cTfh cells, and functional molecule expression. This study provides novel insight showing that mild COVID-19 elicits concerted nAb responses, which are shaped by both Treg and Tfh cells.
关键词：
CeO 2;Matrix redox interference;Hg 2+detection;Phosphatase-mimetic activity;Nanozyme
摘要：
Given heavy metals pose huge threats to ecological system and human health, it is significant to monitor them. With the favorable feature of catalytic signal amplification, redox-type nanozymes have been widely used to detect heavy metals. However, the presence of redox substances in samples can interfere with these catalytic reactions, impacting the accuracy and repeatability of detection. To avoid the situation, here we designed a non-redox nanozyme (3-mercaptopropionic acid modified nanoceria, MPA-CeO2) and developed a novel approach free from matrix redox interference to detect Hg2+. The well-dispersed MPA-CeO2 showed high and stable phosphatase-mimetic activity to catalyze the hydrolysis of colorless p-nitrophenyl phosphate (p-NPP) to yellow p-nitrophenol (p-NP). The addition of Hg2+ could bind specifically onto the MPA-CeO2 particles through S–Hg bond and cause the latter to aggregate, thereby suppressing the ability of MPA-CeO2 with shielded active surfaces to hydrolyze p-NPP. According to the simple principle, highly sensitive and specific colorimetric measurement of Hg2+ was achieved, and excellent reliability and practicability were demonstrated by real sample analysis. To our best knowledge, this study is the first one of detecting Hg2+ using phosphatase-mimicking nanozymes, and it will inspire the exploration of new analytical methods via designing non-redox nanozymes for various targets.
摘要：
The immune response to Mycoplasma pneumoniae infection plays a key role in clinical symptoms. Previous investigations focused on the pro-inflammatory effects of leukocytes and the pivotal role of epithelial cell metabolic status in finely modulating the inflammatory response have been neglected. Herein, we examined how glycolysis in airway epithelial cells is affected by M. pneumoniae infection in an in vitro model. Additionally, we investigated the contribution of ATP to pulmonary inflammation. Metabolic analysis revealed a marked metabolic shift in bronchial epithelial cells during M. pneumoniae infection, characterized by increased glucose uptake, enhanced aerobic glycolysis, and augmented ATP synthesis. Notably, these metabolic alterations are orchestrated by adaptor proteins, MyD88 and TRAM. The resulting synthesized ATP is released into the extracellular milieu via vesicular exocytosis and pannexin protein channels, leading to a substantial increase in extracellular ATP levels. The conditioned medium supernatant from M. pneumoniae-infected epithelial cells enhances the secretion of both interleukin (IL)-1β and IL-18 by peripheral blood mononuclear cells, partially mediated by the P2X7 purine receptor (P2X7R). In vivo experiments confirm that addition of a conditioned medium exacerbates pulmonary inflammation, which can be attenuated by pre-treatment with a P2X7R inhibitor. Collectively, these findings highlight the significance of airway epithelial aerobic glycolysis in enhancing the pulmonary inflammatory response and aiding pathogen clearance.
摘要：
This article provides an overview of the background knowledge of ferroptosis in the nervous system, as well as the key role of nuclear factor E2-related factor 2 (Nrf2) in regulating ferroptosis. The article takes Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), and amyotrophic lateral sclerosis (ALS) as the starting point to explore the close association between Nrf2 and ferroptosis, which is of clear and significant importance for understanding the mechanism of neurodegenerative diseases (NDs) based on oxidative stress (OS). Accumulating evidence links ferroptosis to the pathogenesis of NDs. As the disease progresses, damage to the antioxidant system, excessive OS, and altered Nrf2 expression levels, especially the inhibition of ferroptosis by lipid peroxidation inhibitors and adaptive enhancement of Nrf2 signaling, demonstrate the potential clinical significance of Nrf2 in detecting and identifying ferroptosis, as well as targeted therapy for neuronal loss and mitochondrial dysfunction. These findings provide new insights and possibilities for the treatment and prevention of NDs.
作者机构：
[Tang, Xian; Han, Hai] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;[Li, Le] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Wang, Hongqing] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Hongqing] Univ South China, Hunan key Lab Design & Applicat Actinide Complexes, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Tang, X ; Li, L ] U;Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
Uranium, highly chemically toxic and radioactive with a long half-life, poses environmental risks due to activities like uranium mining, refining, and nuclear power plant operations. Detecting uranyl ion in real-time is crucial to assess human health risks and control uranium pollution. Electrochemical sensing offers advantages such as sensitivity, selectivity, simplicity, rapidity, and cost-effectiveness, making it ideal for on-site detection of uranyl ion. This paper summarizes recent research progress in electrochemical detection of uranyl. Various techniques including cyclic voltammetry, differential pulse voltammetry, stripping voltammetry, and electrochemical impedance spectroscopy are discussed along with their respective advantages. Detection methods utilizing electrode-modified functional materials like biomaterials, polymers, and nanomaterials are investigated. Probes like DNAzyme, ion-imprinted polymers, and 2D materials are highlighted for their detection mechanism and effectiveness. Electrochemical sensing with these probes achieves nanomolar-level detection limits (nM) with excellent stability and selectivity, suggesting practical applications. Lastly, predictions are made regarding sensing mechanisms, electrode material selection, and adaptability to field conditions for electrochemical detection of uranyl ion.
期刊：
INFECTION AND DRUG RESISTANCE,2024年16:1313-1326 ISSN：1178-6973
通讯作者：
Li, G.;Xiao, H.
作者机构：
[Xiao, Hui; Yin, Chunjie; Liu, Mengwen] Xinjiang Med Univ, Sch Publ Hlth, Urumqi, Peoples R China.;[Xu, Miao; Mijiti, Xiaokaiti; Wang, Quan] Xinjiang Med Univ, Affiliated Hosp 8, Urumqi, Peoples R China.;[Zhao, Xiuqin; Liu, Haican; Jiang, Yi; Cao, Bin; Li, Machao; Wan, Kanglin; Li, Guilian] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Collaborat Innovat Ctr Diag & Treatment Infect Dis, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China.;[Cao, Bin] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Anwaierjiang, Aiketaguli] Coll Xinjiang Uyghur Med, Hetian, Peoples R China.
通讯机构：
[Li, G.; Xiao, H.] S;State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China
期刊：
Journal of Assisted Reproduction and Genetics,2024年 ISSN：1058-0468
通讯作者：
Jiang, M
作者机构：
[He, Xueke; Jiang, Miao; Liao, Minjun; Jiang, M; Chen, Hao] Univ South China, Hunan Int Sci & Technol Cooperat Base Arterioscle, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis,Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Zhao, Xiaomei] Univ South China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Dawei] Univ Alberta, Fac Med & Dent, Dept Pediat, Grp Mol & Cell Biol Lipids, Edmonton, AB, Canada.;[Jiang, Zhisheng] Univ South China, Inst Cardiovasc Dis, Dept Pathophysiol,Postdoctoral Res Stn Basic Med, Key Lab Arteriosclerol Hunan Prov,Hunan Int Sci &, Hengyang 421001, Peoples R China.
通讯机构：
[Jiang, M ] U;Univ South China, Hunan Int Sci & Technol Cooperat Base Arterioscle, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis,Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
关键词：
CoQ10;Embryonic development;Germ cells;Mitochondrial electron transport chain (mETC)
摘要：
Coenzyme Q10 (CoQ10) is a natural component widely present in the inner membrane of mitochondria. CoQ10 functions as a key cofactor for adenosine triphosphate (ATP) production and exhibits antioxidant properties in vivo. Mitochondria, as the energy supply center of cells, play a crucial role in germ cell maturation and embryonic development, a complicated process of cell division and cellular differentiation that transforms from a single cell (zygote) to a multicellular organism (fetus). Here, we discuss the effects of CoQ10 on oocyte maturation and the important role of CoQ10 in the growth of various organs during different stages of fetal development. These allowed us to gain a deeper understanding of the pathophysiology of embryonic development and the potential role of CoQ10 in improving fertility quality. They also provide a reference for further developing its application in clinical treatments.
期刊：
Science of The Total Environment,2024年916:170342 ISSN：0048-9697
通讯作者：
Liang, GY;Chen, ZZ
作者机构：
[Liang, GY; Yin, Lihong; Pu, Yuepu; Liang, Geyu; Ge, Yiling; Zhang, Tianyi; Fang, Yifei; Yang, Sheng; Zhu, Yuxin; Wan, Xin; Hu, Chengyu; Gong, Saisai] Southeast Univ, Sch Publ Hlth, Key Lab Environm Med Engn, Minist Educ, Nanjing, Peoples R China.;[Chen, Zaozao; Chen, ZZ] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing, Peoples R China.;[Yang, Fei] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Epidemiol & Hlth Stat,Key Lab Typ Environm Po, Hengyang, Peoples R China.
通讯机构：
[Liang, GY ; Chen, ZZ ] S;Southeast Univ, Sch Publ Hlth, Key Lab Environm Med Engn, Minist Educ, Nanjing, Peoples R China.;Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing, Peoples R China.
关键词：
AOP framework;Ferroptosis;Inhalation exposure;Liver injury and fibrosis;Nanoplastics
摘要：
The emerging contaminant nanoplastics (NPs) have received considerable attention. Due to their tiny size and unique colloidal properties, NPs could more easily enter the body and cross biological barriers with inhalation exposure. While NPs -induced hepatotoxicity has been reported, the hepatic impact of inhaled NPs was still unknown. To close this gap, a 40 nm polystyrene NPs (PS -NPs) inhalation exposure mice model was developed to explore the hepatotoxicity during acute (1 week), subacute (4 weeks), and subchronic period (12 weeks), with four exposure doses (0, 16, 40, and 100 mu g/day). Results showed that inhaled PS -NPs caused a remarkable increase of ALT, AST, and ALP with a decrease of CHE, indicating liver dysfunction. Various histological abnormalities and significantly higher levels of inflammation in a dose- and time -dependent manner were observed. Moreover, after 4 weeks and 12 weeks of exposure, Masson staining and upregulated expression of TGF-beta, alpha-SMA, and Col1a1 identified that inhaled PS -NPs exposure triggered the progression of liver fibrosis with the exposure time prolonged. From the mechanistic perspective, transcriptome analysis revealed that ferroptosis was involved in PS -NPs -induced liver hepatotoxicity, and key features of ferroptosis were detected, including persistent oxidative stress, iron overload, increased LPO, mitochondria damage, and the expression changes of GPX4, TFRC, and Ferritin. And in vitro and in vivo recovery tests showed that ferroptosis inhibitor Fer-1 treatment alleviated liver injury and fibrosis. The above results confirmed the critical role of ferroptosis in PS -NPs -induced hepatotoxicity. Furthermore, to better conclude our findings and understand the mechanistic causality within it, an adverse outcome pathway (AOP) framework was established. In total, this present study conducted the first experimental assessment of inhalation exposure to PS -NPs on the liver, identified that continuous inhaled PS -NPs could cause liver injury and fibrosis, and PS -NPs- ferroptosis provided a novel mechanistic explanation.
摘要：
Patulin (PAT), a fungal metabolite, which mainly exists in various moldy fruits, is greatly harmful to human. However, there are still many unsolved problems in the current detection of PAT. In the work, an ultra-highly sensitive and effective monitoring strategy for PAT was explored based on a novel electrochemiluminescence (ECL) aptasensor. Here, 3, 4, 9, 10-perylenetetracarboxylic acid (PTCA) was chosen as luminophore and terbium (Tb) metal organic gel (MOG) was prepared as a co-reaction accelerator. The MOG displayed a large surface area for immobilization of sufficient PTCA to catalyze co-reactant K2S2O8 to generate more sulfate radical anions (SO4 & BULL; -), thus accelerating the electron-transfer (ET) rate to facilitate ECL efficiency. In the presence of PAT, the ECL signal was greatly quenched, which could be attributed to the specific binding effect between PAT aptamer and PAT. Based on this process, a wide range of PAT concentrations from 0.1 fg & BULL;mL-1 to 0.1 & mu;g & BULL;mL-1 and a low detection limit of 0.02 fg & BULL;mL-1 (S/N = 3) were analyzed. The aptasensor showed excellent detection properties for trace patulin in terms of selectivity, sensitivity, stability, reproducibility and practicability. The designing ideas could serve as a model for the application of metal organic gels to design ultra-highly sensitive ECL platforms for food safety.
关键词：
Actinoplanes maris sp. nov.;polyphasic taxonomy;16S rRNA;marine sediment
摘要：
A novel actinobacterium strain (M4I6(T)) was isolated from marine sediment collected in Megas Gialos, Syros, Greece. On the basis of 16S rRNA gene sequence analysis, strain M4I6(T) was indicated as belonging to the genus Actinoplanes, with high similarity to 'Actinoplanes solisilvae' LAM7112(T) (97.9 %), Actinoplanes ferrugineus IFO 15555(T) (97.6 %), Actinoplanes cibodasensis LIPI11-2-Ac042(T) (97.2 %) and Actinoplanes bogorensis LIPI11-2-Ac043(T) (97.2 %). Phylogenetic analysis of the 16S rRNA gene sequence of strain M4I6(T) showed that the strain formed a stable subclade with 'A. solisilvae' LAM7112T. The cell wall of the novel isolate contained meso- diaminopimelic acid and the whole- cell sugars were xylose, glucose and ribose. The predominant menaquinones were MK- 9(H-4), MK-9(H-2) and MK- 9(H-8). The phospholipid profile comprised phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannosides and an unknown phospholipid. The major fatty acids (>5 %) were anteiso- C-16:0, iso-C-17:0, 10-methyl-C-16:0, C-15:0, iso-C-16: 0 and C-17:0. Genome sequencing showed a DNA G+C content of 70.9 mol%. However, the low average nucleotide identity value, digital DNA-DNA hybridization and average amino acid identity values demonstrated that strain M4I6T could be readily distinguished from its closest related species. Based on data from this polyphasic study, strain M4I6(T) represents a novel species of the genus Actinoplanes, for which the name Actinoplanes maris sp. nov. is proposed. The type strain is M4I6(T) (=DSM 101017(T)=CGMCC 4.7854(T)).
作者机构：
[Liu, Bangxiang; Xia, Changkun; Hu, Panwang; Wang, Mengzhu; Niu, Xiangheng; Zhu, Hengjia] Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China.;[Feng, Rulin] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China.;[Niu, Xiangheng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Changkun Xia; Xiangheng Niu] S;School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, China<&wdkj&>School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China<&wdkj&>School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, China
关键词：
Controllable growth of AgNPs;Hg2+;Smartphone optical analysis;Target-promoted nanozyme activity;Paper-based sensor
摘要：
Given the huge threat of Hg2+ to human health, it is always significant to develop Hg2+ monitoring tools. Utilizing its impacts on nanozyme catalysis is a promising strategy to realize Hg2+ detection. However, nearly all the assays fabricated based on the principle should be performed in solution and in laboratory and require bulky signal reading instruments, hindering their wider applications. It is highly desired to develop portable, efficient, and practical Hg2+ sensors. Herein, we proposed the in situ controllable growth of Ag nanoparticles (AgNPs) on paper to fabricate a smartphone optical nanosensor for highly sensitive Hg2+ detection based on enzyme-mimetic activity stimulation. The ultrasmall AgNPs were directly grown on paper wafers to obtain test strips, which further integrated a 3D-printed holder and an APP built-in smartphone to fabricate the sample-to-answer sensor. The AgNPs-based test strips exhibited a target-stimulated peroxidase-like activity to catalyze the oxidation of 3,3 ',5,5 '-tetramethylbenzidine, and the chromogenic information was captured and processed by the smartphone to rapidly report the level of Hg2+. With the portable nanosensor, highly sensitive and specific detection of Hg2+ was achieved, with a detection limit down to 0.44 nM. The sensor gathers the features of easy construction, simple operation, excellent capability, and robust performance together, promising great potential for rapid onsite analysis.
