A supersensitive electrochemical sensor based on RCA amplification-assisted \"silver chain\"-linked gold interdigital electrodes and CRISPR/Cas9 for the detection of Staphylococcus aureus in food - 成果

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A supersensitive electrochemical sensor based on RCA amplification-assisted "silver chain"-linked gold interdigital electrodes and CRISPR/Cas9 for the detection of Staphylococcus aureus in food

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Zhen, Deshuai;Zhang, Shaoqi;Yang, Aofeng;Ma, Qian;Deng, Zhongliang;...

通讯作者：

He, J

作者机构：

[Zhang, Shaoqi; Deng, Zhongliang; He, Jun; He, J; Zhen, Deshuai] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.

[Fang, Jing; Zhang, Shaoqi; Deng, Zhongliang; Zhen, Deshuai; Yang, Aofeng; Ma, Qian] Univ South China, Coll Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.

[Cai, Qingyun; Zhen, Deshuai] Hunan Univ, Sch Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.

通讯机构：

[He, J ] U

Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.

语种：

英文

关键词：

CRISPR/Cas9;Rolling circle amplification;S. aureus;Silver chain

期刊：

Food Chemistry

ISSN：

0308-8146

年：

2024

卷：

440

页码：

138197

DOI：

10.1016/j.foodchem.2023.138197

基金类别：

National Natural Science Foundation of China [82204176]; Scientific Research Project of Hunan Provincial Health Committee [20201915]; Emergency special project of epidemic prevention control of COVID-19 pneumonia in the University of South China [12]; Hengyang Science and Technology Planning Project [202250045307]; Innovation and Entrepreneurship Training Program for College Students of Hunan Province [S202210555312]

机构署名：

本校为第一且通讯机构

院系归属：

医学院

公共卫生学院

摘要：

With the rising emphasis on food safety, technology to rapidly identify Staphylococcus aureus (S. aureus) is of great significance. Herein, we developed a novel electrochemical biosensor based on the CRISPR/Cas9 system and rolling circle amplification (RCA)-assisted "silver chain"-linked gold interdigital electrodes (Au-IDE). This sensor utilizes RCA to create DNA long chains that span the Au-IDE, and CRISPR/Cas9 as a recognition component to recognize capture/target dsDNA. Additionally, we used silver staining technology to improve detection sensitivity. Then, we detected S. aureus through im...

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A supersensitive electrochemical sensor based on RCA amplification-assisted "silver chain"-linked gold interdigital electrodes and CRISPR/Cas9 for the detection of Staphylococcus aureus in food

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