Synchronous fluorescence determination of urinary 1-hydroxypyrene, β-naphthol and 9-hydroxyphenanthrene based on the sensitizing effect of β-cyclodextrin
作者：
Yang, Hong-Mei;Wang, Yong-Sheng* ;Li, Jun-Hong;Li, Gui-Rong;Wang, Ying;...
期刊：
Analytica Chimica Acta ,2009年636(1):51-57 ISSN：0003-2670
通讯作者：
Wang, Yong-Sheng
作者机构：
[Li, Jun-Hong; Kang, Rong-Hui; Wang, Ying; Tan, Xuan; Li, Gui-Rong; Yang, Hong-Mei; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Mao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Synchronous fluorescence;1-Hydroxypyrene;beta-Naphthol;9-Hydroxyphenanthrene;beta-Cyclodextrin;Urine
摘要：
A novel method for the simultaneous determination of 1-hydroxypyrene (1-OHP), β-naphthol (β-NAP) and 9-hydroxyphenanthrene (9-OHPe) in human urine has been established by using synchronous fluorescence spectrometry. It was based on the fact that synchronous fluorescence spectrometry can resolve the broad-band overlapping of conventional fluorescence spectra, which arise from their similar molecular structures. Only one single scan is needed for quantitative determination of three compounds simultaneously when Δλ = 15 nm is chosen. The signals detected at these three wavelengths, 369.6, 330.0 and 358.0 nm, vary linearly when the concentration of 1-OHP, β-NAP and 9-OHPe is in the range of 2.16 × 10-8-1.50 × 10-5 mol L-1, 1.20 × 10-7-1.10 × 10-5 mol L-1 and 1.07 × 10-7-3.50 × 10-5 mol L-1, respectively. The correlation coefficients for the standard calibration graphs were 0.994, 0.999 and 0.997 (n = 7) for 1-OHP, β-NAP and 9-OHPe, respectively. The limits of detection (LOD) for 1-OHP, β-NAP and 9-OHPe were 6.47 × 10-9 mol L-1, 3.60 × 10-8 mol L-1 and 3.02 × 10-8 mol L-1with relative standard deviations (R.S.D.) of 4.70-6.40%, 2.80-4.20%, 3.10-4.90% (n = 6), respectively. The method described here had been applied to determine traces of 1-OHP, β-NAP and 9-OHPe in human urine, and the obtained results were in good agreement with those obtained by the HPLC method. In addition, the interaction modes between β-cyclodextrin (β-CD) and 1-OHP, β-NAP or 9-OHPe, as well as the mechanism of the fluorescence enhancement were also discussed. © 2009 Elsevier B.V. All rights reserved.
语种：
英文
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Analysis of malachite green and crystal violet in fish with bilinear model
作者：
Yuan, Jin-Tao;Liao, Li-Fu* ;Xiao, Xi-Lin;He, Bo;Gao, Shu-Qin
期刊：
Food Chemistry ,2009年113(4):1377-1383 ISSN：0308-8146
通讯作者：
Liao, Li-Fu
作者机构：
[Gao, Shu-Qin; Liao, Li-Fu; Xiao, Xi-Lin; Yuan, Jin-Tao; He, Bo] Univ S China, Coll Chem & Chem Engn, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Xi-Lin] Hunan Univ, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Liao, Li-Fu] U;Univ S China, Coll Chem & Chem Engn, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
关键词：
Bilinear model;Crystal violet;Fish;Malachite green;Thiocyanate;Visible absorption spectra;Water-ethanol mixed solvent
摘要：
A new method for the simultaneous analysis of malachite green (MG) and crystal violet (CV) in fish samples has been developed. In the presence of thiocyanate, the second order data of MG or CV obtained from the spectra of MG or CV in a series of water-ethanol mixed solvents with various ethanol volume fractions could be expressed as the combination of two bilinear data matrices. With the bilinear model, the second order spectra data of mixtures containing MG and CV those coexist with interferents could be analysed using the second order calibration algorithms, and the simultaneous determination of MG and CV in fish samples could be achieved. The algorithms were based on residual bilinearisation (RBL) and unfolded partial least-squares/residual bilinearisation (U-PLS/RBL) respectively. The method has been successfully used to determine simultaneously MG and CV in some fish samples with satisfactory results. © 2008 Elsevier Ltd. All rights reserved.
语种：
英文
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Resonance light scattering method for the determination of anionic surfactant with acridine orange
作者：
Xiao, Xilin;Wang, Yongsheng* ;Chen, Zhangming;Li, Qiangxiang;Liu, Zhihuo;...
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2008年71(2):398-402 ISSN：1386-1425
通讯作者：
Wang, Yongsheng
作者机构：
[Lue, Changyin; Li, Yanzhi; Wang, Yongsheng; Xiao, Xilin; Li, Guirong; Xue, Jinhua] Univ S China, Coll Publ Hlth, Henyang 421001, Peoples R China.;[Xiao, Xilin] Univ S China, Coll Chem & Chem Engn, Henyang 421001, Peoples R China.;[Chen, Zhangming] Changsha Med Coll, Changsha 410219, Hunan, Peoples R China.;[Liu, Zhihuo; Li, Qiangxiang] Cent S Univ, Xiangya Hosp, Changsha 410219, Hunan, Peoples R China.
通讯机构：
[Wang, Yongsheng] U;Univ S China, Coll Publ Hlth, Henyang 421001, Peoples R China.
关键词：
Anionic surfactant;Acridine orange;Resonance Rayleigh scattering;Resonance nonlinear scattering
摘要：
The resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency-double scattering (FDS) spectra of sodium dodecylbenzene sulfonate (SDBS) (anionic surfactant (AS)) with acridine orange (AO) system were studied. Experimental results showed that when λ<inf>em</inf> = λ<inf>ex</inf> = 537 nm, the RRS peak of AO was greatly enhanced with the increase of SDBS concentration at a pH range of 1.8-4.0. The linear range of the calibration curve for SDBS was 0.028-8.71 mg L<sup>-1</sup> with a detection limit of 8.36 μg L<sup>-1</sup> when the AO concentration was 2.5 ×10<sup>-5</sup>mol L<sup>-1</sup>. The method has been applied to the determination of trace amount of AS in environmental water samples with satisfactory results. In addition, when λ<inf>em</inf> = 321 nm and λ<inf>ex</inf> = 642 nm, the intensity of FDS was proportional to the SDBS concentration ranging from 0.014 to 8.71 mg L<sup>-1</sup> and the correlation coefficient was 0.993 with a detection limit of 4.31 μg L<sup>-1</sup>;when λ<inf>em</inf> = 642 nm and λ<inf>ex</inf> = 321 nm, the intensity of SOS was proportional to the SDBS concentration ranging from 0.050 to 8.71 mg L<sup>-1</sup>, and the correlation coefficient was 0.993 with a detection limit of 14.9 μg L<sup>-1</sup>. ©2008 Elsevier B.V. All rights reserved.
语种：
英文
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A new species of Dactylellina producing adhesive knobs and non-constricting rings to capture nematodes
作者：
Su, Hongyan* ;Hao, Yu'e;Yang, Xiaoyan;Yu, Zefen;Deng, Jingshi;...
期刊：
MYCOTAXON ,2008年105:313-316 ISSN：0093-4666
通讯作者：
Su, Hongyan
作者机构：
[Yang, Xiaoyan; Su, Hongyan] Dali Coll, Dept Biol & Chem, Dali 67000, Peoples R China.;[Hao, Yu'e] Univ S China, Dept Publ Hlth, Hengyang 421001, Peoples R China.;[Yu, Zefen; Mo, Minghe; Deng, Jingshi] Yunnan Univ, Lab Conservat & Utilizat Bioresources, Kunming 650091, Peoples R China.
通讯机构：
[Su, Hongyan] D;Dali Coll, Dept Biol & Chem, Dali 67000, Peoples R China.
关键词：
Antagonist of parasitic nematodes;Biological control;Predacious fungi;Trapping devices
摘要：
A nematode-trapping fungus, Dactylellina daliensis, is illustrated and described as a new species. This fungus is primarily characterized by producing elongate fusoid conidia with 1-13 (mostly 6-8) septa on the denticles of conidiophore and capturing nematodes by means of stalked adhesive knobs and non-constricting rings.
语种：
英文
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吖啶红—共振荧光光谱法测定水溶液中的苯酚
作者：
Tan Guang-Hui;Li Gui-Rong* ;Wang Yong-Sheng;Liu Yun-Fu
期刊：
分析化学 ,2008年36(5):687-690 ISSN：0253-3820
通讯作者：
Li Gui-Rong
作者机构：
[Liu Yun-Fu; Wang Yong-Sheng; Li Gui-Rong; Tan Guang-Hui] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Li Gui-Rong] U;Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
共振荧光;吖啶红;苯酚
摘要：
在硫酸介质中吖啶红的分子共振荧光信号强,发射峰尖而对称,受干扰因素少.基于苯酚能抑制KBrO_3氧化吖啶红的反应,建立了阻抑动力学共振荧光分析法测定痕量苯酚.方法的线性范围为8.5×10~(-4)～1.00 mg/L苯酚,检出限0.26 μg/L.本方法用于环境水样中苯酚的测定,RSD小于2.94%, 样品加标回收率为97.5%～102.5%.
语种：
中文
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Genotoxicity of total and fractionated extractable organic matter in fine air particulate matter from urban Guangzhou: Comparison between haze and nonhaze episodes
作者：
Xu, Haijuan;Wang, Xinming* ;Poesch, Ulrich;Feng, Shaolong;Wu, Dui;...
期刊：
Environmental Toxicology and Chemistry ,2008年27(1):206-212 ISSN：0730-7268
通讯作者：
Wang, Xinming
作者机构：
[Xu, Haijuan; Fu, Jiamo; Sheng, Guoying; Wang, Xinming; Song, Wei] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China.;[Poesch, Ulrich] Max Planck Inst Chem, D-55128 Mainz, Germany.;[Feng, Shaolong] Nanhua Univ, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Wu, Dui] China Meteorol Agcy, Inst Trop & Marine Meteorol, Guangzhou 510080, Peoples R China.;[Yang, Ling] So Med Univ, Nanfang Hosp, Guangzhou 510515, Peoples R China.
通讯机构：
[Wang, Xinming] C;Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China.
关键词：
Fine particles;Genotoxicity;Haze;Extractable organic matter;Comet assay
摘要：
To investigate the genotoxic potencies of extractable organic matter (EOM) in aerosols, fine air particulate matter (PM<inf>2.5</inf>) was collected simultaneously at a roadside (1.2 m above ground) and at a rooftop location (50 m above ground) in urban Guangzhou (China) during a nonhaze period in September 2006 and a haze period in October 2006. Particle-bound organics were extracted and separated into aliphatic, aromatic, and polar fractions. The genotoxicity of total and fractionated extracts were tested by single-cell electrophoresis (comet assay) with human blood lymphocytes. The PM<inf>2.5</inf> concentrations usually exceeded the U.S. National Ambient Air-Quality Standard level (65 μg/m<sup>3</sup>) at both the roadside and the rooftop. During nonhaze days, the roadside samples showed substantially higher PM<inf>2.5</inf> levels (108-130 μg/m<sup>3</sup>) and significantly higher genotoxic effects of total and fractionated EOM (p <0.05 for >10 m<sup>3</sup> air equivalent/ml) than the rooftop samples. During haze days, however, PM <inf>2.5</inf> levels and genotoxic potencies of rooftop samples were drastically elevated and comparable to those of roadside samples, implying that during haze episodes, most people in the urban area are exposed to PM <inf>2.5</inf> pollution as serious as in the heavily polluted roadside microenvironment. All total EOM samples showed significant (p <0.05) dose-response effects, and their effects as olive tail moment were less than the sums of the three fractions. Aromatic fractions of EOM exhibited the greatest genotoxic potencies, but polar fractions also contributed substantially to DNA-damaging effects. Polycyclic aromatic hydrocarbons and nitrated derivatives likely are the most important species responsible for the genotoxicity of EOM in PM<inf>2.5</inf>. ©2008 SETAC.
语种：
英文
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Determination of Sudan I in chilli powder from solvent components gradual change–visible spectra data using second order calibration algorithms
作者：
Yuan, Jintao;Liao, Lifu* ;Lin, Yingwu;Deng, Changai;He, Bo
期刊：
Analytica Chimica Acta ,2008年607(2):160-167 ISSN：0003-2670
通讯作者：
Liao, Lifu
作者机构：
[Yuan, Jintao; Liao, Lifu; Lin, Yingwu; Deng, Changai] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Yuan, Jintao; He, Bo] Univ S China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Parallel factor analysis;Rank annihilation factor analysis;Second order calibration;Solvent components gradual change;Sudan I;Visible absorption spectra
摘要：
It was discovered that a second order spectra data matrix of Sudan I produced from the solvent components gradual change-visible absorption spectra can be expressed as the combination of two bilinear data matrices. Based on this discovery, a new method for the determination of Sudan I in gray systems using second order calibration algorithms has been developed. The second order calibration algorithms were based on the popular parallel factor analysis (PARAFAC) and rank annihilation factor analysis (RAFA), respectively. In the method described here, the components of the solvent were changed gradually by adding ethanol into cyclohexane, the absorption spectra of Sudan I and chilli samples in a series of cyclohexane-ethanol mixed solvents with various ethanol volume fractions were recorded, and then the second order data were obtained from the solvent components gradual change-visible absorption spectra. Thus, the concentration of Sudan I in a gray system could be determined from the spectra matrices using second order calibration algorithms. This method is simple, convenient and dependable. The method has been used to determine Sudan I in chilli powder with satisfactory results. © 2007 Elsevier B.V. All rights reserved.
语种：
英文
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Study on the interaction among pyronine Y, potassium bromate and naphthols by absorption, three-dimension fluorescence and resonance light scattering spectra and their application
作者：
Yang, Hongmei;Wang, Yongsheng* ;Wang, Ying;Li, Junhong;Xiao, Xilin;...
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2008年71(4):1290-1295 ISSN：1386-1425
通讯作者：
Wang, Yongsheng
作者机构：
[Li, Junhong; Wang, Yongsheng; Wang, Ying; Tan, Xuan; Yang, Hongmei] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xilin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Yongsheng] Univ S China, Coll Publ Hlth, W Changsheng Rd 28, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yongsheng] U;Univ S China, Coll Publ Hlth, W Changsheng Rd 28, Hengyang 421001, Peoples R China.
关键词：
Resonance light scattering;Naphthols;PyronineY;Potassium bromate;Urine
摘要：
A novel method for the determination of trace naphthols was proposed based on naphthols, potassium bromate and pyronine Y in dilute sulfuric acid medium can react to form ion-association complexes, which not only resulted in the changes of the three-dimension fluorescence spectra, absorption spectra and the quenching of fluorescence, but also resulted in the great enhancement of resonance light scattering (RLS). The characteristics of RLS spectra, the effective factors and optimum conditions of reaction were studied. It was found that the enhanced intensity of RLS at 396 nm was proportional to the concentration of 1-naphthol and 2-naphthol in the range of 41.4 similar to 3024 ng mL(-1) and 38.3 similar to 3068 ng mL-1, respectively. The urine samples analysis of the relative standard deviation was 3.1-7.1% and the average recovery was 96.0% (n = 6). The present method had been applied to the determination of trace naphthols in human urine, the obtained results were in good agreement with those obtained by the HPLC method; moreover, we had carried on an initial study of the reaction mechanism in terms of spectral characteristics. (C) 2008 Elsevier B.V. All rights reserved.
语种：
英文
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Growth inhibition and induction of G1 phase cell cycle arrest in neuroblastoma SH-SY5Y cell by tri-ortho-cresyl phosphate
作者：
Long, Ding-Xin;Wu, Yi-Jun*
期刊：
Toxicology Letters ,2008年181(1):47-52 ISSN：0378-4274
通讯作者：
Wu, Yi-Jun
作者机构：
[Wu, Yi-Jun; Long, Ding-Xin] Chinese Acad Sci, Inst Zool, Mol Toxicol Lab, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China.;[Long, Ding-Xin] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China.;[Long, Ding-Xin] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Wu, Yi-Jun] Chinese Acad Sci, Inst Zool, Mol Toxicol Lab, State Key Lab Integrated Management Pest Insects, Datunlu Rd, Beijing 100101, Peoples R China.
通讯机构：
[Wu, Yi-Jun] C;Chinese Acad Sci, Inst Zool, Mol Toxicol Lab, State Key Lab Integrated Management Pest Insects, Datunlu Rd, Beijing 100101, Peoples R China.
关键词：
Tri-ortho-cresyl phosphate;Cell proliferation;Cell cycle;Neurotoxicity;SH-SY5Y cell
摘要：
It has been known that tri-ortho-cresyl phosphate (TOCP) can induce delayed neurotoxicity in humans and sensitive animal species: however, it also has influence on the developing central nervous system or differentiating neuronal cells. In this study, the effects of TOCP on cell proliferation and cell cycle regulation and the mechanisms that contribute to this effect were investigated by using human neuroblastoma SH-SY5Y cell line. Treatment of the cells with TOCP suppressed cell proliferation and reduced cell viability in a dose- and time-dependent manner. Analysis of cell cycle profile indicated that TOCP blocked cell cycle progression by arresting the cell cycle at G(1) phase. The data of determination of cell cycle regulated molecules at mRNA and protein levels showed that TOCP decreased cyclin D I and increased p21 expression, while did not affect the p53 and p27 levels. Thus, these results indicated that TOCP might induce potential neurodevelopmental toxicity, and a possible mechanism of this toxicity might be the disturbance of cell proliferation by disrupting cell cycle regulatory proteins cyclin D1 and p21 expression. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
语种：
英文
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Resonance light scattering of 1-hydroxypyrene-ethyl violet-anionic surfactant system and its analytical application
作者：
Ou-Yang, Yun Fu;Wang, Yong Sheng* ;Mi, Xian Wen;Xue, Jin Hua;Wang, Ying
期刊：
Analytical Sciences ,2007年23(5):533-537 ISSN：0910-6340
通讯作者：
Wang, Yong Sheng
作者机构：
[Wang, Yong Sheng; Wang, Ying; Xue, Jin Hua; Wang, YS; Ou-Yang, Yun Fu; Mi, Xian Wen] Nanhua Univ, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong Sheng] N;Nanhua Univ, Sch Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
A novel method for the rapid and sensitive analysis of 1-hydroxypyrene (1-OHP) in human urine has been developed that uses a resonance light scattering (RLS) technique. The assay was based on the interaction of ethyl violet (EV) with 1-hydroxypyrene to form an ion-associate complex, which resulted in the enhancement of RLS intensity and the appearance of new RLS spectra. In the presence of anionic surfactant, the maximum RLS peak of the system was located at 396 nm at pH 8.0. Under the optimum conditions, it was found that the enhanced RLS intensity was directly proportional to the concentration of 1-hydroxypyrene in the range of 4.0 - 982 μg l-1. The detection limit was 1.2 μg l-1 and the recoveries of 1-hydroxypyrene were 92.8 - 102.3% (n = 6). The proposed method was successfully applied to the analysis of human urine samples. The results of 1-hydroxypyrene were in agreement with those obtained by the method of high-performance liquid chromatography. 2007 © The Japan Society for Analytical Chemistry.
语种：
英文
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Triton X-100敏化鱼精蛋白共振能量转移荧光猝灭法测定尿中8-羟基脱氧鸟苷
作者：
Mi Xian-Wen* ;Wang Yong-Sheng;Xue Jin-Hua;Ouyang Yun-Fu;Yang Sheng-Yuan;...
期刊：
光谱学与光谱分析 ,2007年27(8):1570-1573 ISSN：1000-0593
通讯作者：
Mi Xian-Wen
作者机构：
[Mi Xian-Wen] Nanhua Univ, Coll Publ Hlth, Hengyang 421001, Peoples R China.;Nanhua Univ, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.;Huaihua Med Coll, Huaihua 418000, Peoples R China.
通讯机构：
[Mi Xian-Wen] N;Nanhua Univ, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
8-羟基脱氧鸟苷;鱼精蛋白;荧光猝灭;共振能量转移
摘要：
研究了8-羟基脱氧鸟苷与鱼精蛋白之间的共振能量转移的最佳条件,建立了鱼精蛋白共振能量转移荧光猝灭法测定8-羟基脱氧鸟苷的新方法.在301 nm,pH 8.2的Tris-HCl缓冲溶液中,鱼精蛋白的荧光猝灭强度与8-羟基脱氧鸟苷的浓度成正比,工作曲线的线性范围为0.50～7.1 μg·mL~(-1),r=0.996 7,方法的检出限为0.18 μg·mL~(-1),RSD=6.2%,平均加标回收率为104%(n=6).
语种：
中文
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Rapid eye movement sleep deprivation selectively impairs recall of fear extinction in hippocampus-independent tasks in rats
作者：
Fu, J.;Li, P.;Ouyang, X.;Gu, C.;Song, Z.;...
期刊：
Neuroscience ,2007年144(4):1186-1192 ISSN：0306-4522
通讯作者：
Tian, S.
作者机构：
[Tian, S.] Nanhua Univ, Coll Med, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.;Nanhua Univ, Coll Life Sci & Technol, Hengyang 421001, Hunan, Peoples R China.;Nanhua Univ, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Tian, S.] N;Nanhua Univ, Coll Med, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.
关键词：
context;freezing;memory;prefrontal cortex;rats
摘要：
Previous studies have shown that rapid eye movement (REM) sleep deprivation (RSD) exerts a detrimental effect on some memory tasks. However, whether post-learning RSD impairs memory for fear extinction, an important model of inhibitory learning, remains to be elucidated. The present study examined the effects of post-extinction RSD from 0 to 6 h and 6 to 12 h on recall of fear extinction tested 24 h after extinction training. We found that RSD from 0 to 6 h significantly increased freezing when recall of extinction of cued fear was tested in the context in which rats received extinction training whereas RSD from 6 to 12 h had no effect (experiments 1 and 2, two hippocampus-independent memory tasks). RSD at either time point had no effect on freezing when recall of extinction of cued fear was tested in the context different from that in which extinction training occurred (experiment 3, a hippocampus-dependent memory task). Additionally, we observed no effect of RSD at either time point on freezing during recall test for extinction of contextual fear (experiment 4, a hippocampus-dependent memory task). These results suggest that the effects of post-extinction RSD on memory for fear extinction are complex. RSD impairs recall of fear extinction in hippocampus-independent tasks, but does not affect recall of fear extinction in hippocampus-dependent tasks. Our findings extend previous research on the effects of RSD on learning and memory and support the notion that REM sleep is involved in memory process of certain tasks. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.
语种：
英文
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Social Support and Posttraumatic Stress Disorder among Flood Victims in Hunan, China
作者：
Feng, Shuidong;Tan, Hongzhuan* ;Benjamin, Abuaku;Wen, Shiwu;Liu, Aizhong;...
期刊：
Annals of Epidemiology ,2007年17(10):827-833 ISSN：1047-2797
通讯作者：
Tan, Hongzhuan
作者机构：
Cent S Univ, Sch Publ Hlth, Changsha 410008, Hunan, Peoples R China.;Nanhua Univ, Dept Epidemiol, Sch Publ Hlth, Hengyang, Hunan, Peoples R China.;Univ Ottawa, OMNI Res Grp, Dept Obstet & Gynecol, Fac Med, Ottawa, ON, Canada.;Shanxi Med Univ, Dept Hlth Stat, Taiyuan, Shanxi, Peoples R China.;[Tan, Hongzhuan] Cent S Univ, Sch Publ Hlth, Xiangya Rd 110, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Tan, Hongzhuan] C;Cent S Univ, Sch Publ Hlth, Xiangya Rd 110, Changsha 410008, Hunan, Peoples R China.
关键词：
Epidemiology;Floods;Posttraumatic Stress Disorder;Social Support
摘要：
Purpose: To explore the relationship between social support and posttraumatic stress disorder (PTSD) among flood victims. Methods: A cross-sectional survey was carried out in 2000 among individuals who had suffered floods in 1998 in Hunan, China. Multistage sampling was used to select the subjects from the flood-affected areas. PTSD was diagnosed according to DSM-IV criteria, and social support was measured according to a social support rating scale. Data were collected through face-to-face interviews using a structured questionnaire. Multiple logistic regression analysis and confirmatory factor analysis was used to examine the relationship between social support and PTSD. Results: Out of a total of 25,478 subjects interviewed, 2336 (9.7%) were diagnosed as having PTSD. PTSD was significantly associated with total social support (odds ratio [OR] 0.80, 95% confidence interval [CI], 0.78-0.82), subjective support (OR 0.48, 95%CI, 0.44-0.52), and support utilization (OR 0.53, 95%CI, 0.49-0.57). Conclusion: PTSD in flood victims is significantly associated with social support; subjective support and support utilization may play more important roles in mitigating the impact of flood than objective support. © 2007 Elsevier Inc. All rights reserved.
语种：
英文
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Leachates of municipal solid waste incineration bottom ash from Macao: Heavy metal concentrations and genotoxicity
作者：
Feng, Shaolong;Wang, Xinming* ;Wei, Gangjian;Peng, Pingan;Yang, Yun;...
期刊：
Chemosphere ,2007年67(6):1133-1137 ISSN：0045-6535
通讯作者：
Wang, Xinming
作者机构：
[Wang, Xinming] Chinese Acad Sci, State Key Lab Organ Geochem, Guangzhou Inst Geochem, Guangzhou 510640, Peoples R China.;Nanhua Univ, Sch Publ Hlth, Hengyang 421001, Peoples R China.;Nanhua Univ, Sch Life Sci & Technol, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Xinming] C;Chinese Acad Sci, State Key Lab Organ Geochem, Guangzhou Inst Geochem, Guangzhou 510640, Peoples R China.
关键词：
municipal solid waste incineration bottom ash (MSWIBA);toxicity characteristic leaching procedure (TCLP);heavy metal;genotoxicity;micronucleus (MN) assay
摘要：
Heavy metals in municipal solid waste incineration bottom ash (MSWIBA) may leach into soil and groundwater and pose long-term risks to the environment. In this study, toxicity characteristic leaching procedure (TCLP) was carried out on the MSWIBA from Macao. Heavy metals in leachates were determined by inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma atomic emission spectrometry (ICP-AES), and genotoxicity of leachates was also evaluated by micronucleus (MN) assay with Vicia faba root tip cells. The results showed that the concentrations of aluminium (Al), manganese (Mn), cobalt (Co), cadmium (Cd) and mercury (Hg) in the leachates were less than 0.01 mg l<sup>-1</sup>, and those of iron (Fe), copper (Cu) and molybdenum (Mo) were less than 0.1 mg l<sup>-1</sup>. The concentrations of chromium (Cr), zinc (Zn), selemium (Se), strontium (Sr), barium (Ba) and caesium (Cs) were between 0.11 mg l<sup>-1</sup> and 2.19 mg l<sup>-1</sup>. Lead (Pb) concentrations, in particular, reached as high as 19.6 mg l<sup>-1</sup>, significantly exceeding the maximum concentration limit (5 mg l<sup>-1</sup> for lead by TCLP). Compared with the negative group, a significant increase of MN frequencies was observed in the leachate-exposed groups (P <0.05). With the increase of heavy metals in the leachates, the toxic effects on the Vicia faba root tip cells increased, implying that heavy metals were the main factors causing the genotoxic effects. Our results suggested that apart from chemical analysis, bioassays like the MN assay of Vicia faba root tip cells should also be included in a battery of tests to assess the eco-environmental risks of bottom ashes before decisions can be made on the utilization, treatment or disposal. ©2006 Elsevier Ltd. All rights reserved.
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英文
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The ecology of nematode-trapping hyphomycetes in cattle dung from three plateau pastures
作者：
Su, Hongyan;Hao, Yu'e;Mo, Minghe;Zhang, Keqin*
期刊：
Veterinary Parasitology ,2007年144(3-4):293-298 ISSN：0304-4017
通讯作者：
Zhang, Keqin
作者机构：
[Zhang, Keqin] Yunnan Univ, Lab Conservat & Utilizat Bioresources, Kunming 650091, Yunnan, Peoples R China.;Dali Coll, Dept Biol & Chem, Dali 67000, Yunnan, Peoples R China.;Nanhua Univ, Dept Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zhang, Keqin] Y;Yunnan Univ, Lab Conservat & Utilizat Bioresources, Kunming 650091, Yunnan, Peoples R China.
关键词：
Cattle faeces;Fungal diversity;Plateau pasture;Predacious fungi
摘要：
This paper investigated the influence of season and altitude on the occurrence of nematode-trapping fungi in cattle faeces. Six hundred and sixty samples of cattle faeces deposited on three plateau pastures with different altitudes in the west of Yunnan Province, China, were examined in 2004. A total of 17 species of nematode-trapping hyphomycetes were isolated from these samples. The predominant species from all three plateau pastures were Arthrobotrys oligospora, A. musiformis, Monacrosporium ellipsosporum, and M. thaumasium. Species with adhesive networks were the most frequently isolated. Overall, species diversity index was negatively correlated with altitude and was different among seasons within the same site. Levels of diversity were highest in the summer, followed by autumn, spring, and winter. The conidia of the hyphomycetes isolated here germinated normally on medium containing cattle faeces, with species developing adhesive networks having the highest rate of germination. However, the rate of conidial trap (CT) formation was lower in species with adhesive networks than those in other species.
语种：
英文
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Dose-dependent expression changes of early response genes to ionizing radiation in human lymphoblastoid cells
作者：
Long, Xian-Hui;Zhao, Zeng-Qiang;He, Xing-Peng;Wang, Hui-Ping;Xu, Qin-Zhi;...
期刊：
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE ,2007年19(4):607-615 ISSN：1107-3756
通讯作者：
Zhou, Ping-Kun
作者机构：
Beijing Inst Radiat Med, Dept Radiat Toxicol & Oncol, Beijing 100850, Peoples R China.;Nanhua Univ, Coll Publ Hlth, Hengyang 421000, Hunan Province, Peoples R China.;[Zhou, Ping-Kun] Beijing Inst Radiat Med, Dept Radiat Toxicol & Oncol, 27 Taiping Rd, Beijing 100850, Peoples R China.
通讯机构：
[Zhou, Ping-Kun] B;Beijing Inst Radiat Med, Dept Radiat Toxicol & Oncol, 27 Taiping Rd, Beijing 100850, Peoples R China.
关键词：
radiation effect;gene expression;radiation-inducible gene;biomarker
摘要：
The sensitivity of cancer cells as well as normal cells in response to ionizing radiation (IR) is believed to be associated with the early inducible expression of specific genes. Using cDNA microarray technology, here we explored and compared the global transcriptional changes in human lymphoblastoid AHH-1 cells irradiated with 0.05-, 0.2-, 0.5-, 2.0- and 10-Gy doses of gamma-rays 4 h after exposure. A dose as low as 0.05 Gy was efficient in inducing a transcriptional response including the up-regulation of 25 genes, some of which are involved in signal transduction pathways, e.g. BMPR2, GPR124, MAPK8IP2 and AGGF1, and the down-regulation of 18 genes. Expression of some genes was altered only at a specific dose. Most importantly, we discovered a number of radiation-response genes, e.g. DNA repair gene XPC, tumor protein p53 inducible protein 3 gene (TP53I3), immediate early response 5 gene, whose transcriptional levels were increased or depressed by IR in a dose-dependent trend within the dose range 0.05-10 Gy. The dose-dependent induced expression of TP53I3 and XPC was confirmed by Northern blot analyses. Using quantitative real-time PCR, we further confirmed that XPC gene induction was dose dependent as well as time dependent, reaching a peak 4 h post-2 Gy and 10 h post-0.05 Gy. The maximum induced expression level of the XPC gene was higher after 2 Gy (3.2-fold) than 0.05 Gy (1.93-fold). The identification of these radiation-inducible genes, especially those exhibiting a dose-dependent response, not only expands our knowledge of the mechanisms underlying the diverse biological effects induced by IR, but provides candidates for developing novel biomarkers of radiation injury.
语种：
英文
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I_3~-共振瑞利散射法测定孔雀石绿及相关效应分析
作者：
Fan, Xian;Lue, Chang-Yin* ;Liu, Yun-Mei;Wang, Zhi-Xiang;Ning, Ling;...
期刊：
分析化学 ,2007年35(8):1207-1210 ISSN：0253-3820
通讯作者：
Lue, Chang-Yin
作者机构：
[Lue, Chang-Yin] Nanhua Univ, Sch Publ Hlth, Dept Hlth Lab Technol, Hengyang 421001, Peoples R China.;Nanhua Univ, Sch Life Sci & Technol, Hengyang 421001, Peoples R China.
通讯机构：
[Lue, Chang-Yin] N;Nanhua Univ, Sch Publ Hlth, Dept Hlth Lab Technol, Hengyang 421001, Peoples R China.
关键词：
孔雀石绿;碘;共振瑞利散射;基体效应;稳定常数
摘要：
在弱酸性介质中,孔雀石绿与I_3~-形成离子缔合物,并进一步聚集成纳米粒子,在λ=468 nm下,激发产生强烈的共振瑞利散射.在0.012～0.900 μg/L范围内,共振光强度与孔雀石绿的含量成线性关系;线性方程为y=6273.8x+37.1,r=0.9997;本工作研究了反应的适宜条件,建立了共振瑞利法测定孔雀石绿的新方法,方法灵敏度高,检出限为3.6 μg/L.在线性范围内,选择低、中、高3组浓度进行精密度实验,RSD分别为2.3%、1.7%、4.2%(n=11).
语种：
中文
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Resonance light scattering spectra of silver thiocyanate system and its application in analysis
作者：
Lu, CY* ;Han, ZH;Yang, SY
期刊：
中国化学快报：英文版 ,2005年16(8):1063-1066 ISSN：1001-8417
通讯作者：
Lu, CY
作者机构：
[Lu, CY] Nanhua Univ, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;Nanhua Univ, Sch Publ Hlth, Hengyang 421001, Peoples R China.;Inst Labour Hyg & Occupat Dis Human Province, Changsha 410007, Peoples R China.
通讯机构：
[Lu, CY] N;Nanhua Univ, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Resonance light scattering;Silver;Silver thiocyanate
摘要：
A novel determination method of Ag+ was established. In acetic acid-sodium acetate buffer (pH 5.0) medium, Ag+ reacts with SCN - to form AgSCN in the presence of TritonX-100, which results in an increase of resonance light scattering (RLS) and giving a new RLS spectrum. The maximum RLS peak was at 585 nm. The enhancement of resonance light scattering at 585 nm was proportional to the concentration of Ag+ ranging from 0.0045-4.00 μg mL-1 (r=0.9991), and the detection limit was 1.37 ng mL-1 with the recovery of 97.70%-104.80%.
语种：
英文
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催化共振光散射法测定痕量亚硝酸根
作者：
Li, GR* ;Wang, YS;He, DX
期刊：
分析化学 ,2005年33(9):1304-1306 ISSN：0253-3820
通讯作者：
Li, GR
作者机构：
[Li, GR; He, DX; Wang, YS] Nanhua Univ, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Li, GR] N;Nanhua Univ, Sch Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
共振光散射法;亚硝酸根;结晶紫;痕量亚硝酸根;催化作用;测定;反应条件;方法检出限
摘要：
根据磷酸中亚硝酸根对溶解O2氧化I-生成I-3的反应具有明显催化作用,I-3与结晶紫结合使共振光散射(RLS)增强,建立了测定痕量NO-2的催化共振光散射法.考察了体系RLS强度的影响因素,优化了反应条件.最大RLS峰位于690.6 nm波长处,NO-2的浓度在2.20～340 μg/L 范围内与RLS强度增强值呈良好的线性关系,方法检出限为0.68 μg/L.用于环境水样中NO-2测定,与文献方法对照结果满意.
语种：
中文
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甲苯胺蓝共振光散射法测定纳克级脱氧核糖核酸
作者：
Xiao, XL* ;Wang, YS;Li, GR;Lu, CY
期刊：
光谱学与光谱分析 ,2004年24(2):190-193 ISSN：1000-0593
通讯作者：
Xiao, XL
作者机构：
[Li, GR; Wang, YS; Lu, CY; Xiao, XL] Nanhua Univ, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Xiao, XL] N;Nanhua Univ, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
甲苯胺蓝
摘要：
研究了吩噻嗪类染料甲苯胺蓝(TB)与DNA作用的共振光散射光谱，在pH 10～ll的范围内，加入 DNA导致甲苯胺蓝共振光散射增强，在350 nm处，存在一共振光散射增强峰，其强度与DNA浓度呈线性关系，据此建立了一种测定DNA的共振光散射法。对于ctDNA，方法的线性范围为0～900 ng·mL~(-1)，检出限为6．75 ng·mL~(-1)，RSD为3．7％；对于fsDNA，方法的线性范围为0～900 ng·mL~(-1)，检出限为2．99 ng· mL~(-1)，RSD为5．6％．已用于合成样品中DNA的测定。
语种：
中文
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