The optimum conditions for the resonance energy transfer between 8-hydroxy-2'-deoxyguanosine(8-OHdG) and protamine were studied, and a novel method for the determination of 8-OHdG has been developed based on the resonance energy transfer fluorescence quenching effect of protamine. The fluorescence quenching intensity of Ps at 301 nm was proportional to the concentration of 8-OHdG. The linear range of the calibration curve was 0. 50-7.1 μg · mL-1 in the pH 8.2 Tris-HCl buffer solution with the detection limits of 0.18 μg · mL-1. The relative...
