作者机构：
[Hu, Fangyan; Long, Dingxin; Hu, Zehui; Ou, Yiquan; Shi, Shengyuan] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Long, Dingxin] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.
通讯机构：
[Long, Dingxin] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, PR China. Electronic address:
摘要：
Mitochondria-associated endoplasmic reticulum membranes (MAMs) are dynamic suborganelle membranes that physically couple endoplasmic reticulum (ER) and mitochondria to provide a platform for exchange of intracellular molecules and crosstalk between the two organelles. Dysfunctions of mitochondria and ER and imbalance of intracellular homeostasis have been discovered in the research of toxics. Cellular activities such as oxidative stress, ER stress, Ca2+ transport, autophagy, mitochondrial fusion and fission, and apoptosis mediated by MAMs are closely related to the toxicological effects of various toxicants. These cellular activities mediated by MAMs crosstalk with each other. Regulating the structure and function of MAMs can alleviate the damage caused by toxicants to some extent. In this review, we discuss the relationships between MAMs and the mechanisms of toxicological effects, and highlight MAMs as a potential target for protection against toxicants.
作者机构：
[Chen, Ping; Wang, Ying; Li, Sha; Peng, Chenjia] Hunan Normal Univ, Coll Life Sci, Natl & Local Joint Engn Lab Anim Peptide Drug Dev, Changsha 410081, Peoples R China.;[Zhang, Shaoqi; Zhen, Deshuai] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.;[Cai, Qingyun; Zhen, Deshuai] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;[Ge, Lite] Cent South Univ, Xiangya Hosp 2, Dept Neurol, Changsha 410011, Peoples R China.;[Ge, Lite] Hunan Normal Univ, Affiliated Hosp 2, Hunan Prov Key Lab Neurorestoratol, Changsha 410003, Peoples R China.
通讯机构：
[Deshuai Zhen] D;[Ping Chen] T;The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China<&wdkj&>Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, PR China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China
摘要：
The enrichment method of trace phosphotyrosine(pTyr) peptides from complex biological samples is of great concern in pTyr proteomics studies by mass spectrometry. In this study, Er-Nd-TiO2 (Er3+/Nd3+ co-doped TiO2) was designed and successfully synthesized by sol-gel method. The Er-Nd-TiO2 possess high sensitivity (detection limit of 1 x 10-10 M), high selectivity at a low molar ratio of 1:1000:1000 (pTyr peptides: phosphopeptides: non-phosphopeptides), less preferences, and good reusability (8 cycles). The high enrichment efficiency and speci-ficity for phosphotyrosine peptides are ascribed to the synergistic effect of doped Er and Nd, which not only inhibit the growth of TiO2 nanocrystals inducing a high specific surface area but also have strong affinity and coordination ability toward the phosphate groups of peptides. Moreover, the cation-pi interactions between metal cation Er, Nd, Ti and the phenyl ring in an aromatic side chain of tyrosine residue further enhances the specific enrichment of phosphotyrosine peptides by Er-Nd-TiO2. Compared with commercial TiO2, the nanomaterial Er-Nd-TiO2 had better specific adsorption of p-Tyr peptides and could be applied to the enrichment of p-Tyr pep-tides in complex samples.
期刊：
Journal of Medical Virology,2023年95(5):e28797- ISSN：0146-6615
通讯作者：
Yang, ZR;Feng, SD
作者机构：
[Zheng, Chenli; Wang, Xiaohui; Gan, Yongxia; Zhao, Jin; Yang, Zhengrong; Zeng, Guang; Li, Guilian] Shenzhen Ctr Dis Control & Prevent, Dept HIV AIDS Prevent & Control, Shenzhen, Peoples R China.;[Zeng, Guang; Feng, Shuidong; Tang, Jie] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Peoples R China.;[He, Fei] Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Stomatol, Shenzhen, Peoples R China.;[Zhang, Xiaomin] Shenzhen Ctr Dis Control & Prevent, Inst Pathogen Biol, Shenzhen, Peoples R China.;[Xu, Liumei] Southern Univ Sci & Technol, Peoples Hosp Shenzhen 3, Affiliated Hosp 2, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China.
通讯机构：
[Yang, ZR ] S;[Feng, SD ] U;Shenzhen Ctr Dis Control & Prevent, Dept HIV AIDS Prevent & Control, Shenzhen, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Peoples R China.
摘要：
The immunogenicity induced by the third dose of inactivated coronavirus disease 2019 (COVID-19) vaccines in people living with HIV (PLWH) is unclear, and relevant literature is extremely scarce. It is important to add evidence on the humoral immune response induced by the third dose of inactivated COVID-19 vaccine in PLWH. We collected peripheral venous blood for spike receptor binding domain-protein specific immunoglobulin G (S-RBD-IgG) antibody tests at 28 days after the second dose (T(1) ), 180 days after the second dose (T(2) ) and 35 days after the third dose (T(3) ) of inactivated COVID-19 vaccines in PLWH. The differences in S-RBD-IgG antibody levels and specific seroprevalence among T(1) , T(2) , and T(3) time periods were analyzed, and the effects of age, vaccine brand, and CD4(+) T cell count on the levels and specific seroprevalence of S-RBD-IgG antibody induced by the third dose in PLWH were examined. The third dose of inactivated COVID-19 vaccines induced strong S-RBD-IgG antibody responses in PLWH. The levels and specific seroprevalence of S-RBD-IgG antibody were significantly higher than those at 28 and 180 days after the second dose and were not affected by vaccine brand or CD4(+) T cell count. Younger PLWH produced higher levels of S-RBD-IgG antibody. The third dose of inactivated COVID-19 vaccine showed good immunogenicity in PLWH. It is necessary to popularize the third dose in the PLWH population, especially PLWH who do not respond to two doses of inactivated COVID-19 vaccines. Meanwhile, the durability of the protection provided by the third dose in PLWH must be continuously monitored.
摘要：
Cadmium (Cd) contamination of rice is an urgent ecological and agricultural problem. Strontium (Sr) has been shown to promote plant growth. However, the effect of Sr on rice seedlings under Cd stress is currently unclear. In this work hydroponic experiments were used to assess the impact of Sr on rice seedling growth under Cd stress. The findings demonstrated that foliar application of 0.5mgL(-1) Sr had no discernible impact on the development of rice seedlings. However, Sr significantly alleviated growth inhibition and toxicity in rice seedlings when threatened by Cd. Compared with the Cd treatment (Cd, 2.5mgL(-1)), the root length, shoot height, and whole plant length of rice seedlings in the Cd+Sr treatment (Cd, 2.5mgL(-1); Sr, 0.5mgL(-1)) increased by 4.96%, 12.47% and 9.60%, respectively. The content of Cd in rice decreased by 23.34% (roots) and 5.79% (shoots). Sr lessened the degree of membrane lipid peroxidation damage (lower MDA concentration) among the seedlings of rice under Cd stress by controlling the activities of antioxidant enzymes and GSH content. By changing the expression of antioxidant enzyme-encoding genes and downregulating the heavy metal transporter gene (OsNramp5), Sr reduced accumulation and the detrimental effects of Cd on rice seedlings. Our study provides a new solution to the problem of Cd contamination in rice, which may promote the safe production of rice and benefit human health.
摘要：
Mycoplasmas, the smallest known self-replicating organisms, possess a simple structure, lack a cell wall, and have limited metabolic pathways. They are responsible for causing acute or chronic infections in humans and animals, with a significant number of species exhibiting pathogenicity. Although the innate and adaptive immune responses can effectively combat this pathogen, mycoplasmas are capable of persisting in the host, indicating that the immune system fails to eliminate them completely. Recent studies have shed light on the intricate and sophisticated defense mechanisms developed by mycoplasmas during their long-term co-evolution with the host. These evasion strategies encompass various tactics, including invasion, biofilm formation, and modulation of immune responses, such as inhibition of immune cell activity, suppression of immune cell function, and resistance against immune molecules. Additionally, antigen variation and molecular mimicry are also crucial immune evasion strategies. This review comprehensively summarizes the evasion mechanisms employed by mycoplasmas, providing valuable insights into the pathogenesis of mycoplasma infections.
摘要：
Previous studies have primarily concentrated on the hepatotoxicity of MC-LR, whereas its gastric toxicity effects and mechanisms of long-term exposure under low dosage remain unknown. Herein, the gastric tissue from C57BL/6 mice fed with drinking water contaminated by low-dose MC-LR (including 1, 60, and 120 μg/L) was investigated. The results obtained showed that exposure to different concentrations of MC-LR resulted in significant shedding and necrosis of gastric epithelial cells in mice, and a down-regulation of tight junction markers, including ZO-1, Claudin1, and Occludin in the stomach, which might lead to increased permeability of the gastric mucosa. Moreover, the protein expression levels of p-RAF/RAF, p-ERK1/2/ERK1/2, Pink1, Parkin, and LC3-II/LC-3-I were increased in the gastric tissue of mice exposed to 120 μg/L of MC-LR, while the protein expression level of P62 was significantly decreased. Furthermore, we found that pro-inflammatory factors, including IL-6 and TNF-ɑ, were dramatically increased, while the anti-inflammatory factor IL-10 was significantly decreased in the gastric tissue of MC-LR-exposed mice. The activation of the MAPK signaling pathway and mitophagy might contribute to the development of gastric damage by promoting inflammation. We first reported that long-term exposure to MC-LR induced gastric toxicity by activating the MAPK signaling pathway, providing a new insight into the gastric toxic mechanisms caused by MC-LR.
摘要：
Polysaccharide chitosan and L-histidine were applied to synthesize chitosan-based carbon dots (CA-CDs) by a simple laser ablation method. After characterization of the CA-CDs by FT-IR, UV-vis, Raman, XRD, TEM, and XPS, the CA-CDs were introduced as an eco-friendly and high-performance corrosion inhibitor for mild steel (MS) in 1.0M HCl solution. The inhibition action and mechanism of CA-CDs were determined by weight loss and electrochemical measurements, in combination with SEM, AFM, and XPS. The results show that CA-CDs as mixed-type inhibitors could effectively weaken the corrosion of MS in 1.0M HCl solution, and their maximum inhibition efficiency reaches 97.4% at 40mgL(-1). The adsorption behavior of CA-CDs well obeys the Langmuir adsorption isotherm containing both chemisorption and physisorption. The chemisorption mainly results from the multiple adsorption sites in the CA-CDs, and the physical adsorption is due to the blocking and barrier effect of CA-CD nanoparticles. Both adsorption behaviors were proposed to elucidate the corrosion inhibition mechanism of CA-CDs.
期刊：
FRONTIERS IN IMMUNOLOGY,2023年14:1138818 ISSN：1664-3224
作者机构：
[Cao, Bin; Yuan, Xiuqin; Yu, Jinjie] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Qian, Chengyu; Cao, Bin; Zhao, Xiuqin; Wang, Ruihuan; Liu, Haican; Yu, Jinjie; Fan, Xueting; Luan, Xiuli; Li, Guilian; Li, Machao; Wan, Kanglin] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China.;[Qian, Chengyu] Wenzhou Med Univ, Coll Lab Med, Sch Life Sci, Wenzhou, Peoples R China.
关键词：
Mycobacterium tuberculosis;ESAT-6;CFP-10;nPstS1;adjuvant;Recombinant subunit protein vaccine
摘要：
Tuberculosis (TB) is an infectious disease that seriously affects human health. Until now, the only anti-TB vaccine approved for use is the live attenuated Mycobacterium bovis (M. bovis) vaccine - BCG vaccine, but its protective efficacy is relatively low and does not provide satisfactory protection against TB in adults. Therefore, there is an urgent need for more effective vaccines to reduce the global TB epidemic. In this study, ESAT-6, CFP-10, two antigens full-length and the T-cell epitope polypeptide antigen of PstS1, named nPstS1, were selected to form one multi-component protein antigens, named ECP001, which include two types, one is a mixed protein antigen named ECP001m, the other is a fusion expression protein antigen named ECP001f, as candidates for protein subunit vaccines. were prepared by constructing one novel subunit vaccine by mixing or fusing the three proteins and combining them with aluminum hydroxide adjuvant, and the immunogenicity and protective properties of the vaccine was evaluated in mice. The results showed that ECP001 stimulated mice to produce high titre levels of IgG, IgG1 and IgG2a antibodies; meanwhile, high levels of IFN-gamma and a broad range of specific cytokines were secreted by mouse splenocytes; in addition, ECP001 inhibited the proliferation of Mycobacterium tuberculosis in vitro with a capacity comparable to that of BCG. It can be concluded that ECP001 is a novel effective multicomponent subunit vaccine candidate with potential as BCG Initial Immunisation-ECP001 Booster Immunisation or therapeutic vaccine for M. tuberculosis infection.
摘要：
BACKGROUND: Ethionamide (ETH), a structural analogue of isoniazid (INH), is used for treating multidrug-resistant tuberculosis (MDR-TB). Due to the common target InhA, INH and ETH showed cross-resistance in M. tuberculosis. This study aimed to explore the INH and ETH resistant profiles and genetic mutations conferring independent INH- or ETH-resistance and INH-ETH cross-resistance in M. tuberculosis circulating in south of Xinjiang, China. METHODS: From Sep 2017 to Dec 2018, 312 isolates were included using drug susceptibility testing (DST), spoligotyping, and whole genome sequencing (WGS) to analyze the resistance characteristics for INH and/or ETH. RESULTS: Among the 312 isolates, 185 (58.3%) and 127 (40.7%) belonged to the Beijing family and non-Beijing family, respectively; 90 (28.9%) were INH-resistant (INH(R)) with mutation rates of 74.4% in katG, 13.3% in inhA and its promoter, 11.1% in ahpC and its upstream region, 2.2% in ndh, 0.0% in mshA, whilst 34 (10.9%) were ETH-resistant (ETH(R)) with mutation rates of 38.2% in ethA, 26.2% in inhA and its promoter, and 5.9% in ndh, 0.0% in ethR or mshA; and 25 (8.0%) were INH-ETH co-resistant (INH(R)ETH(R)) with mutation rates of 40.0% in inhA and its promoter, and 8% in ndh. katG mutants tended to display high-level resistant to INH; and more inhA and its promoter mutants showed low-level of INH and ETH resistance. The optimal gene combinations by WGS for the prediction of INH(R), ETH(R), and INH(R)ETH(R) were, respectively, katG+inhA and its promoter (sensitivity: 81.11%, specificity: 90.54%), ethA+inhA and its promoter+ndh (sensitivity: 61.76%, specificity: 76.62%), and inhA and its promoter+ndh (sensitivity: 48.00%, specificity: 97.65%). CONCLUSION: This study revealed the high diversity of genetic mutations conferring INH and/or ETH resistance among M. tuberculosis isolates, which would facilitate the study on INH(R) and/or ETH(R) mechanisms and provide clues for choosing ETH for MDR treatment and molecular DST methods in south of Xinjiang, China.
期刊：
Journal of Applied Spectroscopy,2023年89(6):1232-1240 ISSN：0021-9037
通讯作者：
Xilin Xiao<&wdkj&>Lifu Liao
作者机构：
[Yang, Jing; Nie, Lizhen] Hengyang Market Supervis Inspect & Testing Ctr, Hengyang, Peoples R China.;[Li, Qian; Xu, Canhui; Liu, Chen; Zhou, Xin; Liao, Lifu; Nie, Changming] Univ South China, Sch Chem & Chem Engn, Hengyang, Peoples R China.;[Li, Yujie] Univ South China, Sch Resource & Environm & Safety Engn, Hengyang, Peoples R China.;[Xiao, Xilin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang, Hunan, Peoples R China.
通讯机构：
[Xilin Xiao] H;[Lifu Liao] S;Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>School of Chemistry and Chemical Engineering at University of South China, Hengyang, China
摘要：
The resonance light scattering method was used to study the coordination-driven self-assembly reaction of zinc with a bipolar tetradentate ligand and a bipolar bidentate ligand to form a supramolecular polymer. The bipolar tetradentate ligand was N,N'-bis(pyridoxal phosphate)-o-tetraminobiphenyl synthesized by the reaction of pyridoxal phosphate with biphenyltetramine, and the bipolar bidentate ligand was bis-phenanthroline-glutaraldehyde synthesized by the reaction of glutaraldehyde with 5-aminophe-nanthroline. The formation of the supramolecular polymer causes signal enhancement of resonance light scattering. Based on this, we established a new method for the determination of zinc by resonance light scattering. Under optimized experimental conditions, the zinc concentration in the range of 0.3-30 ng/mL showed a good linear relationship with the resonance light scattering intensity of the system. The detection limit of the method is 0.1 ng/mL. Using this method to determine the content of zinc in food, the recovery rate of standard addition is between 95 and 107%, and the relative standard deviation is less than 3.68%.
作者机构：
[Chen, Limou; Feng, Shuidong; Yang, Fei; Tang, Yan] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Key Lab Typ Environm Pollut & Hlth Hazards Hunan P, Hengyang 421001, Peoples R China.;[Shen, Minxue; Chen, Xiang] Cent South Univ, Xiangya Hosp, Dept Dermatol, Changsha 410008, Peoples R China.;[Shen, Minxue; Yang, Fei] Cent South Univ, Xiangya Sch Publ Hlth, Changsha 410000, Peoples R China.
通讯机构：
[Fei Yang] T;The Key Laboratory of Typical Environmental Pollution and Health Hazards of Hunan Province, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Xiangya School of Public Health, Central South University, Changsha, China
作者机构：
[Li, Xing; Lu, Shaorong] Guilin Univ Technol, Sch Mat Sci & Engn, Key Lab New Proc Technol Nonferrous Met & Mat, Minist Educ, Guilin 541004, Peoples R China.;[Li, Xing; Cai, Ren] Hunan Univ, Coll Mat Sci & Engn, State Key Lab Chemo BioSensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Peoples R China.;[Li, Xing; Cai, Ren] Hunan Univ, Collaborat Res Ctr Mol Engn Theranost, Changsha 410082, Peoples R China.;[Yang, Hongfen] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
通讯机构：
[Lu, S.] K;[Cai, R.] M;[Yang, H.] H;Key Laboratory of New Processing Technology for Nonferrous Metals and Materials, China;Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, China
摘要：
A flexible film is designed as a multifunctional sensor with high electrical conductivity, rapid electrothermal response, and high strain sensitivity. Abstract Herein, a flexible film, called PPA film, is designed as a multifunctional sensor with high electrical conductivity, rapid electrothermal response, and high strain sensitivity. The film contains polyvinyl alcohol (PVA), graphene nanosheets (GNs), nanocellulose crystals (CNCs), polydopamine (PDA), and silver nanoparticles (Ag NPs), symbolized as PPA film. The CNCs enhance the dispersion of the GNs during film formation; the stacked GNs change heat conduction and resistance during film bending; PDA serves as a linking agent; Ag NPs improve the conductivity (i.e., 2.65 × 103 S/m) of the film during electrothermal response measurement. A flexible strain sensor designed from PPA films is mounted on the skin surface of human joints to monitor human movement in real time. The sensor exhibited good stability and cycling durability, i.e., the resistance variation remained stable during the bend‐release repetitions for 600 cycles at 1.2 % strain. The flexible sensors exhibited highly sensitive to bending strain, i.e., 30.5 of the gauge factor (GF), and can be applied to monitor bending motions of human joints, such as finger, wrist, elbow, and knee joints.In addition, the PPA films exhibit very high joule heat generation ability and demonstrate its applicability as a joint or stomach warmer and sensor. The as‐designed flexible sensor is a promising candidate for application in environmentally friendly and multifunctional wearable devices.
摘要：
Cyanobacterial blooms are considered a serious global environmental problem. Recent studies provided evidence for a positive association between exposure to microcystin-LR (MC-LR) and cardiotoxicity, posing a threat to human cardiovascular health. However, there are few studies on the cardiotoxic effects and mechanisms of long-term low-dose MC-LR exposure. Therefore, this study explored the long-term toxic effects and toxic mechanisms of MC-LR on the heart and provided evidence for the induction of cardiovascular disease by MC-LR. C57BL/6 mice were exposed to 0, 1, 30, 60, 90, and 120 & mu;g/L MC-LR via drinking water for 9 months and subsequently necropsied to examine the hearts for microstructural changes using H & E and Masson staining. The results demonstrated fibrotic changes, and qPCR and Western blots showed a significant up-regulation of the markers of myocardial fibrosis, including TGF-& beta;1, & alpha;-SMA, COL1, and MMP9. Through the screening of signaling pathways, it was found the expression of PI3K/AKT/mTOR signaling pathway proteins was up-regulated. These data first suggested MC-LR may induce myocardial fibrosis by activating the PI3K/AKT/mTOR signaling pathway. This study explored the toxicity of microcystins to the heart and preliminarily explored the toxic mechanisms of long-term toxicity for the first time, providing a theoretical reference for preventing cardiovascular diseases caused by MC-LR.
摘要：
Inflammatory bowel disease (IBD) is a chronic, lifelong gastrointestinal disease, characterized by periods of activity and remission. The etiology of IBD is closely related to environmental factors. Previous studies have shown that the cyanotoxin microcystin-LR (MC-LR) causes intestinal damage, even IBD. To explore MC-LR's effects and potential mechanisms on IBD occurrence and development, we used dextran-sulfate sodium gavage (DSS) and MC-LR together for the first time in mice. There were four groups of mice: (A) mice given PBS gavage (control, CT); (B) mice given 3% DSS gavage (DSS); (C) mice given 200 & mu;g/kg MC-LR gavage (MC-LR); and (D) mice given 3% DSS + 200 & mu;g/kg MC-LR gavage (DSS + MC-LR). Compared with the CT group, the MC-LR group and the DSS group demonstrated more severe colitis results, which presented as higher weight loss, an increased Disease Activity Index (DAI) score, shorter colon length, a higher degree of tissue structural damage, more apoptotic cells, and greater pro-inflammatory cytokines. Similarly, the DSS + MC-LR group showed more severe colitis compared with the DSS group. Subsequent experiments confirmed that MC-LR or DSS increased the expression of pyroptosis-related proteins mediated by the nucleotide-binding domain-like receptor protein 3 (NLRP3). Likewise, compared with the DSS group, the DSS + MC-LR group expressed these proteins at a higher level. In conclusion, our research is the first to show that MC-LR may induce colitis, and even IBD, through NLRP3 inflammasome-mediated pyroptosis, and it could aggravate DSS-induced colitis in the same way.
期刊：
Journal of Soil Science and Plant Nutrition,2023年23(3):3461-3469 ISSN：0718-9508
通讯作者：
Liu, J
作者机构：
[Ni, Juan; Fan, Xinting; Liu, Jun; Mo, Aili; Jiang, Yuanyuan] Univ South China, Sch Basic Med Sci, Hengyang Med Sch, Dept Cell Biol & Genet, Hengyang 421001, Hunan, Peoples R China.;[Ni, Juan; Fan, Xinting; Liu, Jun; Mo, Aili; Jiang, Yuanyuan] Univ South China, Inst Cytol & Genet, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Liu, Jun] Univ South China, Sch Basic Med Sci, Hengyang Med Sch, Key Lab Ecol Environm & Crit Human Dis Prevent Hun, Hengyang 421001, Hunan, Peoples R China.;[Liu, Jun] Univ South China, Sch Basic Med Sci, Hengyang Med Sch, Key Lab Hengyang City Ecol Impedance Technol Heavy, Hengyang 421001, Hunan, Peoples R China.;[Liu, Jun] Univ South China, Sch Basic Med Sci, Hengyang Med Sch, Key Lab Hengyang City Biol Toxicol & Ecol Restorat, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liu, J ] U;Univ South China, Sch Basic Med Sci, Hengyang Med Sch, Dept Cell Biol & Genet, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Inst Cytol & Genet, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Sch Basic Med Sci, Hengyang Med Sch, Key Lab Ecol Environm & Crit Human Dis Prevent Hun, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Sch Basic Med Sci, Hengyang Med Sch, Key Lab Hengyang City Ecol Impedance Technol Heavy, Hengyang 421001, Hunan, Peoples R China.
关键词：
Cd and Se;Rice;Uptake and distribution;Dietary intake risk assessment
摘要：
PurposeCadmium (Cd) is toxic to rice seedlings. Studies showed that selenium (Se) could alleviate Cd toxicity in rice seedlings. However, the effect of Se on the accumulation of Cd in mature rice plants and Se accumulation in grain and the quality and dietary intake risk of brown rice are rarely investigated.MethodsA rice pot experiment was performed with Se-treated Cd-contaminated paddy soil to explore the effect of Se on Cd accumulation of mature rice plants and the yield and quality of the rice grain, as well as the health risks of consuming rice grains.ResultsAdding Se significantly increased the rice grain yield, decreased Cd concentrations in the tissues of rice plants, and significantly decreased protein and amylose concentrations in brown rice, which helped improve the taste and quality of the cooked rice. Adding Se decreased the health risk index of Cd and increased the daily intake of Se for humans by consuming rice; however, excessive Se addition resulted in Se in rice grain exceeding the Tolerable Upper Intake Level.ConclusionAn appropriate dosage of Se can decrease Cd accumulation in rice, increase yield and Se accumulation, and ensure its edible safety.
通讯机构：
[Hao Liang; Sheng-yuan Yang] D;Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China
摘要：
In this work, ovalbumin-stabilized gold nanoclusters(OVA-Au NCs) fluorescent nanoprobes were synthesized by microwave heating and applied to detect picric acid(PA). The nanoprobes emitted red fluorescence with the maximum fluorescence peak of 680 nm under the excitation...MORE In this work, ovalbumin-stabilized gold nanoclusters(OVA-Au NCs) fluorescent nanoprobes were synthesized by microwave heating and applied to detect picric acid(PA). The nanoprobes emitted red fluorescence with the maximum fluorescence peak of 680 nm under the excitation wavelength of 350 nm, and the Stokes shifts could be up to 330 nm which could effectively eliminate the interference of resonance scattering light. Compared with hydrothermal method, the synthesis method was simple and fast, and only took 50 s. Due to the absorption peak of PA overlapped with the emission peak of OVA-Au NCs in a large range, PA could selectively quench the fluorescence of OVA-Au NCs based on the inner filter effect(IFE) and a quick response time(1 min). Therefore, a new and sensitive method for PA monitoring was established. Under the optimal conditions, the concentration of PA demonstrated a satisfactory linear correlation with the fluorescence quenching degree ?F/F0of the sensing system in the range of 20-240 μmol/L with the detection limit of 6.4 μmol/L. The proposed method is simple, fast, accurate, and easy to realize real-time monitoring.FEWER
