Activation of Kruppel-like factor 6 by multi-walled carbon nanotubes in a diameter-dependent manner in THP-1 macrophages in vitro and bronchoalveolar lavage cells in vivo
作者:
Song, Fengmei;Tang, Xiaomin;Zhao, Weichao;Huang, Chaobo;Dai, Xuyan;...
期刊:
Environmental Science: Nano ,2023年10(3):855-865 ISSN:2051-8153
通讯作者:
Cao, Yi(caoyi39@126.com)
作者机构:
[Song, Fengmei; Tang, Xiaomin; Cao, Yi; Zhao, Weichao] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Huang, Chaobo] Nanjing Forestry Univ NFU, Coll Chem Engn, Nanjing 210037, Peoples R China.;[Dai, Xuyan] Hunan Agr Univ, Econ Coll, Changsha 410128, Peoples R China.
通讯机构:
[Yi Cao] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China
摘要:
While Kruppel-like factors (KLFs) are known to regulate many basic processes of macrophages, their modulatory roles in nanomaterial (NM)-caused bio-effects in macrophages are unclear. Recently we reported diameter-dependent induction of cytotoxicity, inflammation and lipid accumulation by multi-walled carbon nanotubes (MWCNTs) in THP-1 macrophages in vitro, and hereby we further investigated the roles of KLFs. RNA-sequencing data showed that MWCNTs diameter-dependently up-regulated a number of KLFs, including KLF6, a transcription factor (TF) mediating inflammatory macrophage polarization. Furthermore, MWCNTs influenced KLF6-related gene ontology (GO) terms and downstream genes (identified by ingenuity pathway analysis), typically those related with inflammation and TF functions. We further verified that only MWCNTs of the smallest diameter promoted soluble monocyte chemotactic protein 1 (sMCP-1) proteins, while KLF6 siRNA remarkably inhibited C-C motif chemokine ligand 2 (CCL2, MCP-1 encoding gene) mRNA. Similarly, the TF involved in endoplasmic reticulum (ER) stress, activating transcription factor 3 (ATF3), was activated by MWCNTs with the smallest diameter and inhibited by KLF6 siRNA. However, KLF6 knockdown did not obviously alter lipid levels in MWCNT-exposed macrophages. In mice receiving repeated intratracheal instillation of MWCNTs (128 μg per mouse, once a week for 5 weeks), MWCNTs of the smallest diameter induced inflammatory responses in lungs and promoted the expression of KLF6, CCL2, ATF3 and nitric oxide synthase 2 (nos2) in bronchoalveolar lavage cells. Our results suggested a previously unknown role of KLF6 in regulating MWCNT-induced inflammatory responses both in vitro and in vivo. © 2023 The Royal Society of Chemistry.
语种:
英文
展开
Malic Enzyme 1 as a Novel Anti‐Ferroptotic Regulator in Hepatic Ischemia/Reperfusion Injury (Adv. Sci. 13/2023)
作者:
Fang, Xuexian;Zhang, Jiawei;Li, You;Song, Yijing;Yu, Yingying;...
期刊:
Advanced Science ,2023年10(13):e2205436 ISSN:2198-3844
通讯作者:
Fang, XX;Wang, FD
作者机构:
[Yang, Jun; Zhang, Jiawei; Li, You; Song, Yijing; Lian, Fuzhi; Fang, Xuexian] Hangzhou Normal Univ, Sch Publ Hlth, Dept Nutr & Toxicol, Key Lab Elemene Class Anticanc Chinese Med Zhejian, Hangzhou 311121, Peoples R China.;[Min, Junxia; Wang, Fudi; Yu, Yingying] Zhejiang Univ, Affiliated Hosp 2, Affiliated Hosp 1, Inst Translat Med,Sch Med,State Key Lab Expt Hemat, Hangzhou 310058, Peoples R China.;[Wang, Fudi] Univ South China, Affiliated Hosp 1, Sch Publ Hlth, Hengyang Med Sch,Basic Med Sci, Hengyang 421001, Peoples R China.
通讯机构:
[Wang, FD ] Z;[Fang, XX ] H;Hangzhou Normal Univ, Sch Publ Hlth, Dept Nutr & Toxicol, Key Lab Elemene Class Anticanc Chinese Med Zhejian, Hangzhou 311121, Peoples R China.;Zhejiang Univ, Affiliated Hosp 2, Affiliated Hosp 1, Inst Translat Med,Sch Med,State Key Lab Expt Hemat, Hangzhou 310058, Peoples R China.;Univ South China, Affiliated Hosp 1, Sch Publ Hlth, Hengyang Med Sch,Basic Med Sci, Hengyang 421001, Peoples R China.
关键词:
ferroptosis;hepatic ischemia;reperfusion injury;malate;malic enzyme 1;nicotinamide adenine dinucleotide phosphate
摘要:
Ferroptosis has been linked to the pathogenesis of hepatic injury induced by ischemia/reperfusion (I/R). However, the mechanistic basis remains unclear. In this study, by using a mouse model of hepatic I/R injury, it is observed that glutathione (GSH) and cysteine depletion are associated with deficiency of the reducing power of nicotinamide adenine dinucleotide phosphate (NADPH). Genes involved in maintaining NADPH homeostasis are screened, and it is identified that I/R-induced hepatic ferroptosis is significantly associated with reduced expression and activity of NADP(+)-dependent malic enzyme 1 (Me1). Mice with hepatocyte-specific Me1 gene deletion exhibit aggravated ferroptosis and liver injury under I/R treatment; while supplementation with L-malate, the substrate of ME1, restores NADPH and GSH levels and eventually inhibits I/R-induced hepatic ferroptosis and injury. A mechanistic study further reveals that downregulation of hepatic Me1 expression is largely mediated by the phosphatase and tensin homologue (PTEN)-dependent suppression of the mechanistic target of rapamycin/sterol regulatory element-binding protein 1 (mTOR/SREBP1) signaling pathway in hepatic I/R model. Finally, PTEN inhibitor, mTOR activator, or SREBP1 over-expression all increase hepatic NADPH, block ferroptosis, and protect liver against I/R injury. Taken together, the findings suggest that targeting ME1 may provide new therapeutic opportunities for I/R injury and other ferroptosis-related hepatic conditions.
语种:
英文
展开
Green and rapid synthesis of biomass carbon dot-based fluorescence sensing for the sensitive determination of oxytetracycline
作者:
Li, Qianji;Wu, Xiwen;Zhang, Xiaohuan;Su, Wenen;Tan, Yan;...
期刊:
Analytical Methods ,2023年15(12):1569-1575 ISSN:1759-9660
通讯作者:
Liu, Jinquan(letliufly@163.com);Yang, Shengyuan(yangshyhy@126.com)
作者机构:
[Yang, Shengyuan; Tan, Yan; Su, Wenen; Liu, Jinquan; Fan, Pengfei; Li, Qianji] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.;[Wu, Xiwen] Yueyang Cty Ctr Dis Control & Prevent, Yueyang 414100, Hunan, Peoples R China.;[Zhang, Xiaohuan] Yongding Dist Ctr Dis Control & Prevent, Zhangjiajie 427000, Hunan, Peoples R China.
通讯机构:
[Li, Qianji] D;Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, People's Republic of China.
摘要:
Eco-friendly biomass carbon dots (CDs) with blue fluorescence emission were rapidly synthesized by a microwave method. Based on the inner filter effect (IFE) between oxytetracycline (OTC) and CDs, the fluorescence of CDs could be selectively quenched by OTC. Therefore, a simple and time-saving fluorescence sensing system for the detection of OTC was established. Under optimal experimental conditions, the concentration of OTC showed a good linear relationship with fluorescence quenching values (ΔF) in the range of 4.0-100.0 μmol L−1, a corresponding correlation coefficient (r) of 0.9975, and a detection limit of 0.12 μmol L−1. The method has the advantages of low cost, time-saving, and green synthesis that could be used for the determination of OTC. Moreover, possessing high sensitivity and specificity, this fluorescence sensing method was successfully applied for detecting OTC in milk, indicating its potential applications in food safety. © 2023 The Royal Society of Chemistry
语种:
英文
展开
Enhancement of microbial current production by riboflavin requires the reduced heme centers in outer membrane cytochromes in Shewanella oneidensis MR-1
作者:
Huang, Wenyuan;Long, Xizi;Okamoto, Akihiro
期刊:
Electrochimica Acta ,2023年464:142860 ISSN:0013-4686
通讯作者:
Okamoto, A
作者机构:
[Huang, Wenyuan; Okamoto, Akihiro] Natl Inst Mat Sci, Res Ctr Macromol & Biomat, 1-1 Namiki, Tsukuba, Ibaraki 3050044, Japan.;[Huang, Wenyuan; Long, Xizi; Okamoto, Akihiro] Natl Inst Mat Sci, Int Ctr Mat Nanoarchitecton, 1-1 Namiki, Tsukuba, Ibaraki 3050044, Japan.;[Huang, Wenyuan; Okamoto, Akihiro] Hokkaido Univ, Grad Sch Chem Sci & Engn, North 13 West 8, Kita Ku, Sapporo, Hokkaido 0608628, Japan.;[Okamoto, Akihiro] Univ Tsukuba, Coll Sci & Engn, Grad Sch Sci & Engn, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058573, Japan.;[Okamoto, Akihiro] Tokyo Inst Technol, Living Syst Mat LiSM Res Grp, Int Res Frontiers Initiat IRFI, 4259 Nagatsuta, Midori Ku, Yokohama 2268503, Japan.
通讯机构:
[Okamoto, A ] N;Natl Inst Mat Sci, Res Ctr Macromol & Biomat, 1-1 Namiki, Tsukuba, Ibaraki 3050044, Japan.
关键词:
Extracellular electron transfer;Microbial fuel cells;Circular dichroism;Differential pulse voltammetry;Semiquinone
摘要:
The rate enhancement of extracellular electron transfer is critical for microbial fuel cells (MFCs). Flavins have been a promising additive to enhance current production even with a few µM through the formation of bound semiquinone cofactor in the outer membrane cytochromes (OMCs). However, the effect of flavins vastly decreases upon the dissociation from OMCs to be soluble electron shuttle. Therefore, identifying a critical factor to stabilize the bound flavin cofactor is essential. Herein, we demonstrated that the reduced heme centers in OMCs promote riboflavin (RF) binding to OMCs by modulating the intracellular electron pathways in Shewanella oneidensis MR-1. UV–Vis and circular dichroism spectroscopy in situ showed that fumarate or dimethyl sulfoxide (DMSO) oxidizes heme centers in OMCs even at low concentrations with lactate as an electron donor, resulted in the defect of current enhancement by RF. Differential pulse voltammetry detected more soluble flavins and less bound semiquinone in the presence of fumarate or DMSO in the wild type. However, mutant strains lacking a reductase for fumarate or DMSO recovered the effect of RF. These results strongly suggest that reduced heme centers promote RF to bind OMCs, and alternative electron acceptors suppress power generation in MFCs even more than the stoichiometric ratio. © 2023 Elsevier Ltd
语种:
英文
展开
Mechano-control of Extracellular Electron Transport Rate via Modification of Inter-heme Coupling in Bacterial Surface Cytochrome
作者:
Long, Xizi;Tokunou, Yoshihide;Okamoto, Akihiro
期刊:
Environmental Science & Technology ,2023年57(19):7421-7430 ISSN:0013-936X
通讯作者:
Okamoto, Akihiro(okamoto.akihiro@nims.go.jp)
作者机构:
[Long, Xizi] Univ South China, Sch Key Lab Typ Environm Pollut & Hlth Hazards Hun, Sch Basic Med, Sch Publ Hlth,Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Tokunou, Yoshihide; Long, Xizi; Okamoto, Akihiro] Int Ctr Mat Nanoarchitecton WPI MANA, Natl Inst Mat Sci, Tsukuba, Ibaraki 3050044, Japan.;[Tokunou, Yoshihide] Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki 3058577, Japan.;[Okamoto, Akihiro] Hokkaido Univ, Grad Sch Chem Sci & Engn, Sapporo, Hokkaido 0608628, Japan.
通讯机构:
[Akihiro Okamoto] I;International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan<&wdkj&>Graduate School of Chemical Sciences and Engineering, Hokkaido University, North 13 West 8, Kitaku, Sapporo, Hokkaido 060-8628, Japan
关键词:
circular dichroism;electron transport;exciton coupling;mechanobiology;multi-heme cytochrome C
摘要:
Bacterial outer-membrane multi-heme cytochromes (OMCs) mediate extracellular electron transport (EET). While heme alignment dictates the rate of EET, control of inter-heme coupling in a single OMC remains challenging, especially in intact cells. Given that OMCs diffuse and collide without aggregation on the cell surface, the overexpression of OMCs could increase such mechanical stress to impact the OMCs’ protein structure. Here, the heme coupling is modified via mechanical interactions among OMCs by controlling their concentrations. Employment of whole-cell circular dichroism (CD) spectra of genetically engineered Escherichia coli reveals that the OMC concentration significantly impacts the molar CD and redox property of OMCs, resulting in a 4-fold change of microbial current production. The overexpression of OMCs increased the conductive current across the biofilm on an interdigitated electrode, indicating that a higher concentration of OMCs causes more lateral inter-protein electron hopping via collision on the cell surface. The present study would open a novel strategy to increase microbial current production by mechanically enhancing the inter-heme coupling. © 2023 American Chemical Society.
语种:
英文
展开
大豆蛋白金纳米簇“关-开”型荧光探针检测炭疽杆菌生物标志物DPA
作者:
Qian Duo;Su Wen-en;Liu Zhi-yuan;Gao Xiao-yu;Yi Yu-xin;...
期刊:
光谱学与光谱分析 ,2023年43(6):1815-1820 ISSN:1000-0593
通讯作者:
Yang, SY
作者机构:
[Gao Xiao-yu; Qian Duo; Hu Cong-cong; Yang Sheng-yuan; Liu Zhi-yuan; Liu Bin; Su Wen-en; Yi Yu-xin] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构:
[Yang, SY ] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
关键词:
大豆蛋白金纳米簇;2,6-吡啶二羧酸;生物标志物;炭疽杆菌;荧光探针
摘要:
炭疽杆菌是高致病性病原微生物, 引起的炭疽病在我国属于乙类传染病, 因此建立操作简便、 灵敏准确的炭疽杆菌检测方法对预防和控制炭疽传播, 维护公共卫生安全至关重要。 该研究创新性地提出以绿色材料大豆蛋白为保护剂和还原剂, 采用微波加热法合成了一种具有强烈红色荧光发射的大豆蛋白金纳米簇(SPI-AuNCs)。 采用TEM、 UV-Vis、 FL、 XPS、 FTIR等方法表征了SPI-AuNCs的成功合成和部分特殊性能。 结果表明SPI-AuNCs呈球形, 粒径大小在1.8~3.2 nm范围内, 平均粒径为2.65 nm, 在500~550 nm范围内未出现表面等离子体共振峰; SPI-AuNCs的最大激发波长为370 nm, 最大发射波长为680 nm。 SPI-AuNCs的表面官能团主要有—NH、 —COOH、 —OH、 —SH等官能团, 元素的组成主要包括C、 N、 O、 S、 Au元素。 根据Cu2+与SPI-AuNCs表面基团通过配位作用形成非荧光复合物, 使荧光猝灭; 而2,6-吡啶二羧酸(DPA)与Cu2+具有更高的亲和力, 可将Cu2+从SPI-AuNCs表面竞争下来, 使荧光恢复, 据此建立了一种荧光“关-开”策略检测DPA的新方法。 在最佳实验条件下, 荧光恢复率(ΔF/F1)与DPA的浓度在1.15~70.0 μmol·L-1范围内呈良好的线性关系, 线性方程为ΔF/F1=0.011c+0.131, 相关系数r=0.991, 方法检出限为0.34 μmol·L-1。 同时, 通过加标回收实验研究了湖水和牛奶样品中DPA, 得到加标回收率在97.3%~103.6%, 表明了该方法在环境和食品样本DPA的检测中具有很大的应用潜力, 可以为环境监测和食品安全提供方法学支持。 Bacillus anthracis is a highly pathogenic microorganism. Anthrax is an infectious disease caused by Bacillus anthracis, classified as Class B of our country’s statutory reporting infectious diseases. Therefore, the establishment of simple operation, rapid and sensitive detection methods for Bacillus anthracis is vital for preventing and contralling the spread of anthrax and maintaining public health security. This study innovatively proposed to synthesize soy protein gold nanoclusters (SPI-AuNCs) with strong red fluorescence emission by microwave heating using the green material soy protein as a protective agent and reducing agent.TEM, XPS, FTIR, FL, and UV-Vis characterizations were used to verify successful synthesis of SPI-AuNCs. SPI-AuNCs are spherical, which the particle size is in the range of 1.8~3.2 nm, an average diameter of 2.65 nm, and no surface plasma resonance absorption from 500 to 550 nm. The maximum excitation wavelength of SPI-AuNCs is 370 nm, and the maximum emission wavelength appears at 680 nm. The surface functional groups of SPI-AuNCs mainly include —NH, —COOH, —OH, —SH, and the element composition includes C, N, O, S, Au elements. The fluorescence of SPI-AuNCs could be quenched by the coordination between Cu2+ and surface groups of SPI-AuNCs, while DPA has a stronger chelation effect with Cu2+, which could compete for Cu2+ from the surface of SPI-AuNCs and restore the fluorescence of SPI-AuNCs. Accordingly, a new method for DPA detection based on the fluorescent “off-on” strategy was established. Under the optimal experimental conditions, the fluorescence recovery efficiency (ΔF/F1) performs a good linear relationship with DPA concentration in the range of 1.15~70.0 μmol·L-1. The linear regression equation is ΔF/F1=0.011c+0.131 with high correlation coefficient (r=0.991), and the detection limit is 0.34 μmol·L-1. In addition, the spiking experiments of the DPA in lake water and milk samples were performed. The spiked recoveries were 97.3%~103.6%, indicating that this method has great application potential in DPA detection for environmental and food samples, and can provide methodological guidance for environmental monitoring and food safety.
语种:
中文
展开
Amorphous Fe-Containing Phosphotungstates Featuring Efficient Peroxidase-like Activity at Neutral pH: Toward Portable Swabs for Pesticide Detection with Tandem Catalytic Amplification
作者:
Zhu, Hengjia;Liu, Bangxiang;Wang, Mengzhu;Pan, Jianming;Xu, Lizhang;...
期刊:
Analytical Chemistry ,2023年95(10):4776-4785 ISSN:0003-2700
通讯作者:
Pan, Jianming(pjm@ujs.edu.cn);Xu, Lizhang(justxlz@ujs.edu.cn);Niu, Xiangheng(niuxiangheng@ujs.edu.cn)
作者机构:
[Xu, Lizhang; Niu, Xiangheng; Zhu, Hengjia] Jiangsu Univ, Sch Agr Engn, Zhenjiang 212013, Peoples R China.;[Pan, Jianming; Liu, Bangxiang; Hu, Panwang; Wang, Mengzhu; Niu, Xiangheng; Zhu, Hengjia] Jiangsu Univ, Inst Green Chem & Chem Technol, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China.;[Niu, Xiangheng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构:
[Jianming Pan] I;[Lizhang Xu; Xiangheng Niu] S;School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China
摘要:
Peroxidase-mimetic materials are intensively applied to establish multienzyme systems because of their attractive merits. However, almost all of the nanozymes explored exhibit catalytic capacity only under acidic conditions. The pH mismatch between peroxidase mimics in acidic environments and bioenzymes under neutral conditions significantly restricts the development of enzyme-nanozyme catalytic systems especially for biochemical sensing. To solve this problem, here amorphous Fe-containing phosphotungstates (Fe-PTs) featuring high peroxidase activity at neutral pH were explored to fabricate portable multienzyme biosensors for pesticide detection. The strong attraction of negatively charged Fe-PTs to positively charged substrates as well as the accelerated regeneration of Fe2+ by the Fe/W bimetallic redox couples was demonstrated to play important roles in endowing the material with peroxidase-like activity in physiological environments. Consequently, integrating the developed Fe-PTs with acetylcholinesterase and choline oxidase led to an enzyme-nanozyme tandem platform with good catalytic efficiency at neutral pH for organophosphorus pesticide response. Furthermore, they were immobilized onto common medical swabs to fabricate portable sensors for paraoxon detection conveniently based on smartphone sensing, showing excellent sensitivity, good anti-interference capacity, and low detection limit (0.28 ng/mL). Our contribution expands the horizon of acquiring peroxidase activity at neutral pH, and it will also open avenues to construct portable and effective biosensors for pesticides and other analytes. © 2023 American Chemical Society.
语种:
英文
展开
Competition-Based Two-Dimensional Photonic Crystal Dually Cross-Linked Supramolecular Hydrogel for Colorimetric and Fluorescent Dual-Mode Sensing of Bisphenol A
作者:
Du, Wenfang;Liu, Jie;Li, Hong;Deng, Chenyi;Luo, Jie;...
期刊:
Analytical Chemistry ,2023年95(8):4220-4226 ISSN:0003-2700
通讯作者:
Xiao, Fubing(xiaofubing2008@163.com)
作者机构:
[Liu, Jie; Yang, Shengyuan; Du, Wenfang; Tan, Yan; Li, Hong; Luo, Jie; Feng, Qianqian; Deng, Chenyi; Xiao, Fubing] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci,Hunan Key Lab Typ Environm, Hengyang 421001, Peoples R China.;[Du, Wenfang; Wu, Zhaoyang; Xiao, Fubing] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;[Du, Wenfang] Binzhou Univ, Coll Biol & Environm Engn, Binzhou 256600, Peoples R China.
通讯机构:
[Fubing Xiao] H;Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
摘要:
Bisphenol A (BPA), one of the most abundantly produced endocrine disrupting chemicals, is widely used in everyday plastic products and thus must be monitored. Multimode sensing platforms are able to combine the advantages of different strategies while solving the issues of inaccurate test results of single signal sensing. However, the exploration in this field is limited due to the compromise of sensing conditions and inevitable mutual interferences of different systems. Herein, we constructed a two-dimensional photonic crystal dually cross-linked supramolecular hydrogel (2DPCDCSH) by utilizing a host-guest pair of β-cyclodextrin (β-CD) and tert-butyl (t-Bu) as the second cross-linking for colorimetric and fluorescent dual-mode sensing of BPA. Based on the fact that BPA can act as a competitive guest to break the host-guest interaction between β-CD and t-Bu, the cross-linking density decreased and an expansion-induced structural color change occurred. Sensitive and selective BPA detection can be easily achieved by measuring the Debye diffraction ring diameter or observing the color change of 2DPC with a detection limit of 1 μg mL-1. Moreover, the formation of the β-CD/BPA complex gave a significant enhancement of the intrinsic fluorescence of BPA, obtaining a detection limit of 0.001 μg mL-1. The two sensing systems can share the same reaction condition and yield a wider dynamic response range than the single signal strategy. Overall, the proposed method presented an efficient, rapid, cost-effective, and regenerative dual-mode method for BPA analysis and shed new insights for the design of diversified sensing platforms. © 2023 American Chemical Society.
语种:
英文
展开
Strategy of In Situ Electrochemical Regulation for Highly Enhanced Nonenzymatic Sensing of Carbaryl
作者:
Lv, Yitao;Zhang, Yi;Yang, Yunyin;Li, Jingyan;Wang, Jiacheng;...
期刊:
Analytical Chemistry ,2023年95(8):4015-4023 ISSN:0003-2700
通讯作者:
Xiao, Xilin(xiaoxilin@usc.edu.cn);Zhang, Min(mzhang@nwafu.edu.cn)
作者机构:
[Yang, Yunyin; Lv, Yitao; Li, Jingyan; Zhang, Yi; Zhang, Min] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China.;[Wang, Jiacheng] Yangzhou Univ, Med Coll, Yangzhou 225009, Jiangsu, Peoples R China.;[Xiao, Xilin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Xilin Xiao] D;[Min Zhang] C;College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China<&wdkj&>Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China
摘要:
Specific and sensitive sensing of most pesticide residues relies on enzymes such as acetylcholinesterase and advanced materials, which need to be loaded on the surface of working electrodes, leading to instability, uneven surface, tedious process, and high cost. Meanwhile, employing certain potential or current in electrolyte solution could also modify the surface in situ and overcome these drawbacks. However, this method is only regarded as electrochemical activation widely applied in the pretreatment of electrodes. In this paper, by means of regulating the electrochemical technique and its parameters, we prepared a proper sensing interface and derivatized the carbaryl (a carbamate pesticide) hydrolyzed form (1-naphthol) to enhance sensing by 100 times within several minutes. After regulation I by chronopotentiometry with 0.2 mA for 20 s or chronoamperometry with 2 V for 10 s, abundant oxygen-containing groups form and the ordered carbon structure is destroyed. Sweeping from −0.5 to 0.9 V through cyclic voltammetry for only one segment, following regulation II, the composition of oxygen-containing groups changes and the disordered structure is alleviated. Finally, on the constructed sensing interface, test by regulation III through differential pulse voltammetry from 0.8 to −0.4 V, resulting in derivatization of 1-naphthol during 0.8-0 V, followed by electroreduction of the derivative at around −0.17 V. Compared with the electro-oxidation peak at 0.5 V in previous reports, it is essential to improve specificity, even toward several other carbamate pesticides with similar structures. Hence, the in situ electrochemical regulation strategy has demonstrated great potential for effective sensing of electroactive molecules. © 2023 American Chemical Society.
语种:
英文
展开
Green synthesis of CQDs for determination of iron and isoniazid in pharmaceutical formulations
作者:
Yu, Wenzhan;Li, Qian;He, Liqiong;Zhou, Renlong;Liao, Lifu;...
期刊:
Analytical Methods ,2023年15(7):944-950 ISSN:1759-9660
通讯作者:
Xue, Jinhua(862774797@qq.com);Xiao, Xilin(xiaoxl2001@163.com)
作者机构:
[Yu, Wenzhan; Xiao, Xilin] Univ South China, Sch Pharmaceut Sci, Hengyang 421001, Hunan, Peoples R China.;[Li, Qian; Liao, Lifu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[He, Liqiong; Zhou, Renlong; Xue, Jinhua] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Jinhua Xue; Xilin Xiao] S;School of Public Health, University of South China, Hengyang 421001, Hunan, PR China<&wdkj&>School of Pharmaceutical Science, University of South China, Hengyang 421001, Hunan, PR China
摘要:
Camphor leaves were used as the precursor for the hydrothermal synthesis of carbon quantum dots. The preparation method is simple and rapid, and the raw material is environmentally friendly and easy to obtain. Without additional modification, the carbon quantum dots were used as fluorescent probes for the sensitive and selective detection of Fe3+ and isoniazid at different excitation wavelengths. For Fe3+, at the excitation wavelength of 320 nm, the ratio of fluorescence intensity of CQD solution after adding Fe3+ to CQD solution without Fe3+ addition, F/F0, and Fe3+ concentration showed a good linear relationship in the range of 2.72 × 10−5 to 1.00 × 10−4 mol L−1 (R2 = 0.9912), and the limit of detection was 8.16 μmol L−1. For isoniazid, at the excitation wavelength of 270 nm, the ratio of fluorescence intensity of CQDs solution with isoniazid to CQDs solution without isoniazid, F/F0, and isoniazid concentration showed good linear relationships in the range of 3.81 × 10−6 to 1.00 × 10−5 mol L−1 (R2 = 0.9941) and 1.00 × 10−5 to 2.10 × 10−4 mol L−1 (R2 = 0.9910) respectively, and the limit of detection was 1.14 μmol L−1. A fluorescence method for the determination of Fe and isoniazid content was proposed. The method has been used to detect iron in iron supplement tablets and isoniazid in isoniazid tablets with satisfactory results. © 2023 The Royal Society of Chemistry.
语种:
英文
展开
Molecular cloning and oxidative-stress responses of a novel Phi class glutathione S-transferase (GSTF) gene in the freshwater algae Closterium ehrenbergii
作者:
Abassi, Sofia;Wang, Hui;Kim, Hansol;Ki, Jang-Seu*
期刊:
Environmental Toxicology ,2022年37(4):789-801 ISSN:1520-4081
通讯作者:
Ki, Jang-Seu
作者机构:
[Abassi, Sofia; Wang, Hui; Ki, Jang-Seu; Kim, Hansol] Sangmyung Univ, Dept Biotechnol, Seoul 03016, South Korea.;[Wang, Hui] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang, Peoples R China.
通讯机构:
[Ki, Jang-Seu] S;Sangmyung Univ, Dept Biotechnol, Seoul 03016, South Korea.
关键词:
Closterium ehrenbergii;glutathione transferase;green algae;oxidative stress;reactive oxygen species
摘要:
Glutathione S-transferases (GSTs) belong to a family of enzymes involved in diverse biological processes, including detoxification and protection against oxidative damage. Here, we determined the full-length sequence (915 bp) of a novel Phi class cytosolic glutathione S-transferase (GSTF) gene from the green algae Closterium ehrenbergii. We examined the gene structure and expression patterns in response to metals and endocrine disrupting chemicals (EDCs). It was significantly upregulated by metals, but responded differently to EDCs. The highest up-regulation of CeGSTF was registered under 0.1 mg/L CuCl2 and 0.01 mg/L CuSO4 treatments. In a 72-h course experiment with treatment of 0.1 mg/L CuCl2, CeGSTF was dramatically induced at 6 h, and then gradually decreased with increasing exposure time. This was consistent with the increase in both GST activity and ROS production in copper-treated cells. These results suggest that CeGSTF may be involved in detoxification mechanisms associated with oxidative stress in green algae. © 2021 Wiley Periodicals LLC.
语种:
英文
展开
Colloidal templating of highly ordered porous amidoxime-functionalized hydrogel for intelligent treatment of uranium contaminated water
作者:
Xiao, Fubing;Li, Hong;Xie, Peng;Liu, Jie;Du, Wenfang* ;...
期刊:
Chemical Engineering Journal ,2022年431:134141 ISSN:1385-8947
通讯作者:
Du, Wenfang;Yang, SY
作者机构:
[Liu, Jie; Yang, Shengyuan; Du, Wenfang; Li, Hong; Xie, Peng; Li, Le; Xiao, Fubing; Du, WF; Yang, SY] Univ South China, Hengyang Med Sch, Sch Publ Hlth, Hunan Key Lab Typical Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Du, Wenfang; Wu, Zhaoyang; Xiao, Fubing] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;[Du, Wenfang] Binzhou Univ, Coll Biol & Environm Engn, Binzhou 256600, Peoples R China.
通讯机构:
[Du, WF; Yang, SY ] U;Univ South China, Hengyang Med Sch, Sch Publ Hlth, Hunan Key Lab Typical Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
关键词:
Amidoxime;Highly ordered porous;Hydrogel;Uranium;Intelligent treatment
摘要:
Uranium is the key element in the nuclear industry, but it often triggers serious risk when discharged into the natural environment. It is extremely urgent to construct a novel material that can realize intelligent treatment of uranium contaminated water. To this end, a hydrophilic amidoxime-functionalized porous colloidal crystal hydrogel (HAPCCH) is developed in this work for the effective adsorption and detection of uranyl ion (UO22+). HAPCCH with merits of abundant specific UO22+-binding sites of amidoxime ligands and excellent hydrophilicity achieves to a maximum adsorption capacity of 2.52 mmol g−1. The HAPCCH performs well for UO22+ removal in real water samples and shows good regeneration. In addition, sensitive and selective detection of UO22+ can be easily realized by recording the diffraction shift or observing the color change of HAPCCH, achieving a detection limit of 10 nM. The density functional theory (DFT) calculation results reveal that the lower binding free energy and Gibbs free energy of HAPCCH to UO22+ contribute to the better selectivity compared to other ions. The result indicates that this absorbent material holds great potential application in the water pollution control. © 2021 Elsevier B.V.
语种:
英文
展开
Bifunctional Mn-Doped N-Rich Carbon Dots with Tunable Photoluminescence and Oxidase-Mimetic Activity Enabling Bimodal Ratiometric Colorimetric/Fluorometric Detection of Nitrite
作者:
Wang, Mengzhu;Zhu, Hengjia;Liu, Bangxiang;Hu, Panwang;Pan, Jianming;...
期刊:
ACS Applied Materials & Interfaces ,2022年14(39):44762-44771 ISSN:1944-8244
通讯作者:
Niu, Xiangheng(niuxiangheng@ujs.edu.cn)
作者机构:
[Pan, Jianming; Liu, Bangxiang; Hu, Panwang; Wang, Mengzhu; Niu, Xiangheng; Zhu, Hengjia] Jiangsu Univ, Inst Green Chem & Chem Technol, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China.;[Niu, Xiangheng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构:
[Xiangheng Niu] I;Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China
关键词:
Mn-doped carbon dots;dual-mode sensing;multifunctional nanozyme;nitrite;ratiometric colorimetric/fluorometric detection
摘要:
Multimodal detection is a promising paradigm because of its advantages of expanding usage scenarios and improving reliability. However, it is very challenging to design reasonable strategies to achieve the multimodal sensing of targets. Herein, we developed an unprecedented bimodal ratiometric colorimetric/fluorometric method by exploring a novel bifunctional artificial oxidase mimic, Mn-doped N-rich carbon dots (Mn-CDs), to achieve the high-performance determination of nitrite in complicated matrices. The Mn-CDs exhibited both tunable photoluminescence and high oxidase-like activity, effectively catalyzing the colorless 3,3′,5,5′-Tetramethylbenzidine (TMB) oxidation to generate blue TMB+. When nitrite was introduced, the TMB+species generated would specifically react with nitrite to produce diazotized TMB+, resulting in a color change from blue to green and finally to yellow. Simultaneously, the fluorescence of Mn-CDs was quenched by the diazotized TMB+product via the inner filter effect. Hence, the existence of nitrite could lead to the simultaneous variations of visual color and photoluminescence, providing the principal basis for the bimodal ratiometric colorimetric/fluorometric quantification of the target. With the method, excellent sensitivity, selectivity, reliability, and practicability for nitrite detection were verified. Our work proposes a new bimodal strategy for nitrite measurement using bifunctional CDs-based enzyme mimics, which will inspire future effort on the exploration of promising multifunctional nanozymes and their advanced applications in biochemical sensing. © 2022 American Chemical Society. All rights reserved.
语种:
英文
展开
Microcystin-LR Combined with Cadmium Exposures and the Risk of Chronic Kidney Disease: A Case-Control Study in Central China
作者:
Feng, Shuidong;Deng, Shuxiang;Tang, Yan;Liu, Ying;Yang, Yue;...
期刊:
Environmental Science & Technology ,2022年56(22):15818-15827 ISSN:0013-936X
通讯作者:
Shen, Minxue(shenmx1988@csu.edu.cn);Yang, Fei(yangfeilong@126.com)
作者机构:
[Tang, Peng; Deng, Shuxiang; Liu, Ying; Feng, Shuidong; Yang, Fei; Tang, Yan] Univ South China, Sch Basic Med, Sch Publ Hlth, Hengyang Med Sch,Dept Epidemiol & Hlth Stat,Key L, Hengyang 421001, Peoples R China.;[Xu, Shuaishuai; Duan, Yanying; Shen, Minxue; Wei, Jia; Yang, Fei; Yang, Yue] Cent South Univ, Xiangya Sch Publ Hlth, Dept Social Med & Hlth Management, Hunan Prov Key Lab Clin Epidemiol, Changsha 410000, Peoples R China.;[Lu, Yao] Cent South Univ, Xiangya Hosp 3, Ctr Clin Pharmacol, Changsha 410000, Peoples R China.;[Pu, Yuepu; Liang, Geyu; Yang, Fei] Southeast Univ, Sch Publ Hlth, Key Lab Environm Med Engn, Minist Educ, Nanjing 210000, Peoples R China.;[Chen, Xiang; Shen, Minxue] Cent South Univ, Xiangya Hosp, Dept Dermatol, Changsha 410000, Peoples R China.
通讯机构:
[Shen, M.] H;[Yang, F.] D;Hunan Provincial Key Laboratory of Clinical Epidemiology, China;Department of Epidemiology and Health Statistics, China
关键词:
microcystin-LR;chronic kidney disease;cadmium;case-control study;synergistic effect
摘要:
Increasing evidence indicates that exposure to microcystin-LR (MC-LR) can cause kidney damage. However, the association between MC-LR exposure and chronic kidney disease (CKD) risk in humans has not been studied. Therefore, we conducted a population-based case-control study involving 135 CKD cases and 135 matched controls in central China and analyzed the effects of MC-LR alone as well as combined with the known risk factor cadmium (Cd). Compared to the lowest quartile of MC-LR exposure, the highest quartile had a 6.56-fold (95% confidence interval [CI]: 2.46, 17.51) significantly increased risk for CKD, displaying a dose-response relationship (ptrend< 0.001). Our animal study also showed that MC-LR exposure induced kidney injury via the PI3K/AKT/mTOR signaling pathway. Comparing the highest Cd quartile to the lowest, the adjusted odds ratio for CKD was 3.88 (95% CI: 1.47, 10.28), exhibiting a dose-response relationship (ptrend< 0.006). Furthermore, a positive additive interaction was observed between MC-LR and Cd (relative excess risk due to interaction = 1.81, 95% CI: 0.42, 3.20; attributable proportion of interaction = 0.70, 95% CI: 0.35, 1.05). Our study firstly revealed that MC-LR exposure is an independent risk factor for CKD and has a synergistic relationship with Cd. MC-LR and Cd exposures are associated with CKD risk in a dose-response manner. © 2022 American Chemical Society. All rights reserved.
语种:
英文
展开
Effects of epigallocatechin gallate on the stability, dissolution and toxicology of ZnO nanoparticles
作者:
Cao, Wandi;Gu, Manyu;Wang, Shuyi;Huang, Chaobo;Xie, Yixi* ;...
期刊:
Food Chemistry ,2022年371 ISSN:0308-8146
通讯作者:
Xie, Yixi;Cao, Yi
作者机构:
[Xie, Yixi; Wang, Shuyi; Xie, YX; Cao, Yi; Gu, Manyu; Cao, Wandi] Xiangtan Univ, Coll Chem, Key Lab Environm Friendly Chem & Applicat, Minist Educ, Xiangtan 411105, Peoples R China.;[Huang, Chaobo] Nanjing Forestry Univ NFU, Coll Chem Engn, Nanjing 210037, Peoples R China.;[Cao, Yi] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.
通讯机构:
[Xie, YX; Cao, Y] X;Xiangtan Univ, Coll Chem, Key Lab Environm Friendly Chem & Applicat, Minist Educ, Xiangtan 411105, Peoples R China.
关键词:
3D Caco-2 spheroids;Dysfunction of organelles;Epigallocatechin gallate (EGCG);Transcriptomics;ZnO nanoparticles (NPs)
摘要:
Previously we reported the cytoprotective effects of polyphenols rich in hydroxyl groups against ZnO nanoparticles (NPs). This study used RNA-sequencing to evaluate the toxicity of ZnO NPs and epigallocatechin gallate (EGCG) to 3D Caco-2 spheroids. EGCG altered the colloidal stability of ZnO NPs, shown as the changes of atomic force microscopic height, solubility in cell culture medium, and hydrodynamic sizes. EGCG almost completely reversed ZnO NP-induced cytotoxicity, and consistently, alleviated ZnO NP-induced gene ontology (GO) terms and genes related with apoptosis. EGCG also modestly decreased intracellular Zn ions and changed GO terms and genes related with endocytosis/exocytosis in ZnO NP-exposed spheroids. Meanwhile, EGCG changed ZnO NP-induced alteration of GO terms and genes related with the functions of mitochondria, endoplasmic reticulum and lysosomes. We concluded that EGCG alleviated the cytotoxicity of ZnO NPs to 3D Caco-2 spheroids by altering NPs’ colloidal properties and the pathways related with internalization and organelle dysfunction. © 2021 Elsevier Ltd
语种:
英文
展开
TiO2 nanosheets promote the transformation of vascular smooth muscle cells into foam cells in vitro and in vivo through the up-regulation of nuclear factor kappa B subunit 2
作者:
Li, Shuang;Yan, Dejian;Huang, Chaobo;Yang, Fei;Cao, Yi*
期刊:
Journal of Hazardous Materials ,2022年424(Pt D):127704- ISSN:0304-3894
通讯作者:
Cao, Yi
作者机构:
[Li, Shuang; Yang, Fei; Cao, Yi] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.;[Huang, Chaobo; Li, Shuang] Nanjing Forestry Univ NFU, Coll Chem Engn, Nanjing 210037, Peoples R China.;[Li, Shuang; Cao, Yi] Xiangtan Univ, Coll Chem, Key Lab Environm Friendly Chem & Applicat, Minist Educ,Lab Biochem, Xiangtan 411105, Peoples R China.;[Yan, Dejian] North China Elect Power Univ, Inst Adv Mat, Beijing 102206, Peoples R China.
通讯机构:
[Cao, Yi] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.
关键词:
*Lipid droplet biogenesis;*Nuclear factor kappa B subunit 2 (NFKB2);*TiO(2) nanosheets (NSs);*Transcriptomics;*Vascular smooth muscle cells (VSMCs)
摘要:
Titanium dioxide (TiO2) nanomaterials have been shown to promote atherosclerosis through endothelial dysfunction. This study investigated the toxicity of TiO2 nanosheets (NSs) to vascular smooth muscle cells (VSMCs), one of the pivotal cells involved in all stages of atherosclerosis. Only a high concentration of TiO2 NSs (128 μg/mL) modestly induced cytotoxicity by decreasing thiols. RNA-sequencing data revealed that 64 μg/mL TiO2 NSs significantly down-regulated 94 genes and up-regulated 174 genes, respectively. Gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to SMC function and lipid metabolism were altered. TiO2 NSs increased nuclear factor kappa B subunit 2 (NFKB2), which led to a decrease in VSMC marker actin alpha 2, smooth muscle (ACTA2). On the other hand, macrophage marker CD36 and fatty acid synthase (FASN) proteins were increased. Additionally, TiO2 NSs induced inflammatory cytokines and lipid accumulation, and these effects were curtailed by NFKB inhibitor - triptolide. Furthermore, repeated TiO2 NS injection (5 mg/kg BW, once a day for 5 continuous days) into ICR mice led to increased NFKB2, CD36 and FASN, with a decreased ACTA2. Our results suggested that TiO2 NSs promoted the transformation of VSMCs into foam cells through the up-regulation of NFKB2. © 2021 Elsevier B.V.
语种:
英文
展开
Ultrasensitive label-free electrochemical biosensor for detecting linear microcystin-LR using degrading enzyme MlrB as recognition element
作者:
Li, Yanfang;Si, Sisi;Huang, Feiyu;Wei, Jia;Dong, Shengyi;...
期刊:
Bioelectrochemistry ,2022年144:108000- ISSN:1567-5394
通讯作者:
Yang, Fei(phfyang@csu.edu.cn)
作者机构:
[Li, Yanfang; Si, Sisi; Li, Huimin; Liu, Song] Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha;410082, China;[Yang, Fei] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang;421001, China;[Huang, Feiyu; Wei, Jia] Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha
通讯机构:
[Yang, F.] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, China
关键词:
Electrochemical biosensor;Linear microcystin-LR;MlrB;Multi-walled carbon nanotube;Square wave voltammetry
摘要:
A label-free electrochemical biosensor was firstly constructed to detect linear microcystin-LR (L-MC-LR) with high sensitivity. Degradation enzyme MlrB was used as recognition element for specific recognition of L-MC-LR. The electrode was modified with –COOH functionalized multi-walled carbon nanotube to increase the specific surface area and improve the conductivity, which was then applied to immobilize MlrB. The electrochemical signal was changed with the reaction between MlrB and L-MC-LR, which was recorded by using square wave voltammetry. The electrochemical biosensor showed superior sensitivity, with a dynamic range of 1 pg/mL to 100 ng/mL and a detection limit of 0.127 pg/mL. Moreover, the fabricated electrochemical biosensor exhibited excellent specificity toward L-MC-LR in real water samples. The concentrations of spiked L-MC-LR were 0.100, 5.00, 50.0 ng/mL, and the recovery rates were 95.0–104% with relative standard deviation (RSD) of 0.900–2.30% and 74.0–93.0% with RSD of 2.30–3.50% in lake water and tap water, respectively. Furthermore, the selectivity, reproducibility, and stability demonstrated the potential of degradation enzymes as recognition element in detection of cyanotoxins. © 2021 Elsevier B.V.
语种:
英文
展开
Value of noncontrast computer tomography in predicting the characteristics of obstructive uropathy
作者:
Qin, Long;Xu, Jieru;Tang, Yunhui;Zhang, Hu;Yi, Xuan;...
期刊:
Clinical Imaging ,2022年82:53-57 ISSN:0899-7071
通讯作者:
Li, Mingyong
作者机构:
[Fu, Xiaowen; Zhu, Guoqiang; Yi, Xuan; Zhang, Hu; Hu, Wei; Qin, Long; Tang, Yunhui; Li, Mingyong; Jin, Wei] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Urol Dept, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.;[Xu, Jieru] Univ South China, Sch Publ Hlth, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Li, Mingyong] U;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Urol Dept, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.
关键词:
Noncontrast computed tomography;CT values;Nonpyogenic hydronephrosis;Pyonephrosis;Urinary tract infection
摘要:
Objective: To explore the diagnostic value of noncontrast computed tomography (NCCT) in differentiating pyonephrosis from nonpyogenic hydronephrosis on the basis of CT values (in Horsfield unit [HU]). Methods: Data from patients diagnosed with obstructive uropathy at the First affiliated hospital of University of South China from November 2017 to January 2021 were subjected to retrospective analysis. In accordance with the gold standard—the presence of pus during the operation—all patients were divided into the nonpyogenic hydronephrosis group and the pyonephrosis group. The relationship between CT values and the presence or absence of pyonephrosis was performed using binary logistic regression. A receiver operating characteristic (ROC) curve was constructed to determine threshold values for classification on the basis of mean HU. Results: A total of 207 patients, including 100 males and 107 females, were enrolled. Out of the 207 cases, 124 cases of obstructive uropathy were nonpyogenic hydronephrosis and 83 cases were of pyonephrosis. The CT values of the pyonephrosis group were significantly higher than that of the nonpyogenic hydronephrosis group (t = 9.15, P < 0.05). The CT values were dependent on the presence or absence of pyonephrosis (P < 0.05). A HU threshold value of 9.75 could be applied to diagnose the presence of pyonephrosis. Conclusion: The CT values of hydronephrosis might predict the presence of pyonephrosis in the kidney, and the CT value of 9.75 HU might be the appropriate threshold for its prediction. © 2021 Elsevier Inc.
语种:
英文
展开
Construction and Research of Multiple Stimuli-Responsive 2D Photonic Crystal DNA Hydrogel Sensing Platform with Double-Network Structure and Signal Self-Expression
作者:
Chen, Qianshan;Wang, Shihong;Huang, Ting;Xiao, Fubing;Wu, Zhaoyang;...
期刊:
Analytical Chemistry ,2022年94(14):5530-5537 ISSN:0003-2700
通讯作者:
Wu, Zhaoyang(zywu@hnu.edu.cn)
作者机构:
[Wu, Zhaoyang; Yu, Ruqin; Wang, Shihong; Huang, Ting; Chen, Qianshan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;[Xiao, Fubing] Univ South China, Coll Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.
通讯机构:
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China
摘要:
The stimuli-responsive DNA hydrogel has attracted wide attention in the fields of chemical and biological sensing. However, it is still a challenge to integrate characteristics with low-cost, high mechanical strength, and signal self-expression into a DNA hydrogel simultaneously. Herein, a stimuli-responsive 2D photonic crystal double network DNA hydrogel (2D PhC DN-DNA hydrogel) sensing platform is developed via combining the signal self-expression of 2D PhC array with the selective recognition of polyacrylamide (PAM)/DNA DN hydrogel. The change of DNA configuration induced by specific target triggers the change of 2D PhC DN-DNA hydrogel volume, leading to a shift of the Debye diffraction ring diameter. In order to verify the feasibility of this strategy, the 2D PhC DN-DNA hydrogel with C-rich sequences is chosen as a proof-of-concept. The results indicate that the hydrogel has good detection performance for pH and Ag+/Cys. And the Debye diffraction ring diameter of the hydrogel is correlated with the concentration of the Ag+/Cys in the range of 0.5-20 μM. Compared with previously pure DNA hydrogel sensing platform, the 2D PhC DN-DNA hydrogel features low-cost preparation process and label-free determination. Meanwhile, only a laser pointer and a ruler are needed for the determination of targets, which shows that the hydrogel has application prospect in the development of portable response equipment. © 2022 American Chemical Society. All rights reserved.
语种:
英文
展开
Highly sensitive and efficient fluorescent sensing for Hg2+ detection based on triple-helix molecular switch and exonuclease III-assisted amplification
作者:
Zhong, Miao;Yang, Shengyuan;Chen, Lei;Liu, Chen;Shi, Jiao;...
期刊:
Analytica Chimica Acta ,2022年1205:339751 ISSN:0003-2670
通讯作者:
Liu, JQ
作者机构:
[Zhong, Miao; Yang, Shengyuan; Liu, Jinquan; Shi, Jiao; Li, Le; Chen, Lei; Liu, JQ; Liang, Hao; Xiao, Xilin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.;[Zhong, Miao; Yang, Shengyuan; Liu, Jinquan; Shi, Jiao; Li, Le; Chen, Lei; Liang, Hao; Xiao, Xilin] Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Hunan, Peoples R China.;[Liu, Chen] Univ South China, Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构:
[Liu, JQ ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.
关键词:
Mercury (Hg2+);Thymine-mercury-thymine;Triple-helix molecular switch;Exonuclease III;Fluorescence
摘要:
Here, a novel fluorescent sensing for simple, highly sensitive and efficient detection of Hg2+ was developed as joint result of triple-helix molecular switch (THMS) and exonuclease III (Exo III)-assisted signal amplification. In this study, the special structure of THMS was used to realize efficient fluorescence quenching and excellent signal unit transformation to complete the output of signal FAM. In the absence of Hg2+, hairpin probe (HP) containing thymine-rich (T-rich) ssDNA strand can induce the dissociation of the THMS, causing FAM far away from BHQ1 and increasing fluorescence intensity. Nevertheless, Hg2+ could bind to the thymine (T) base to form the dsDNA with T-Hg2+-T structure that stimulates Exo III to digest it from the blunt 3′-terminus to 5′-terminus, causing Hg2+ to be released from the dsDNA. The released Hg2+ could initiate the next cycling, allowing a large number of hairpin probes to be cleaved by Exo III to form ssDNA. These ssDNA could inhibit the switch dissociation of THMS, causing a dramatic decrease in the fluorescence signal. This allowed for the highly sensitive detection of Hg2+ at concentrations as low as 1.04 pM. In addition, the sensing showed a linear detection range of 0.01–50 nM and was used for the assay of Hg2+ in real samples of Xiangjiang river water and tap water. These results showed that the provided fluorescent sensing has a good application prospect in environmental and food monitoring. © 2022
语种:
英文
展开