Simultaneous spectrophotometric determination of trace amount of malachite green and crystal violet in water after cloud point extraction using partial least squares regression
作者：
An, Lin;Deng, Jian* ;Zhou, Liang;Li, Hui;Chen, Fei;Wang, Hui;Liu, Yating
期刊：
Journal of Hazardous Materials ,2010年175(1-3):883-888 ISSN：0304-3894
通讯作者：
Deng, Jian
作者机构：
[Liu, Yating; Chen, Fei; An, Lin; Wang, Hui; Li, Hui; Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Zhou, Liang] Univ S China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Cloud point extraction<&wdkj&>Spectrophotometry<&wdkj&>Malachite green<&wdkj&>Crystal violet<&wdkj&>Partial least squares regression
摘要：
In this work, a new method has been proposed to simultaneously determine the trace amount of malachite green and crystal violet from aqueous solution by spectrophotometry after cloud point extraction (CPE) using partial least squares regression. The optimal extraction and operating conditions, such as pH, reagents concentration and effect of time and temperature, and so on, have been investigated using the non-ionic surfactant Triton X-114. The maximum absorption wavelength for malachite green and crystal violet is 624 and 579 nm, respectively;linearity is obeyed in the range of 9.9-800 and 16-1000 ng mL<sup>-1</sup> with detection limit of 2.9 and 4.8 ng mL<sup>-1</sup>, and the root mean square error of prediction (RMSEP) are 0.0197 and 0.0343, respectively. The proposed method has been applied successfully to simultaneously determine the trace amount of malachite green and crystal violet in real matrix samples with the recoveries of 92.45-102.5%. ©2009 Elsevier B.V. All rights reserved.
语种：
英文
展开
TiO2 nanoparticles co-doped with silver and nitrogen for antibacterial application
作者：
Yuan, Yali* ;Ding, Jianqiang;Xu, Jinsheng;Deng, Jian;Guo, Jianbo
期刊：
Journal of Nanoscience and Nanotechnology ,2010年10(8):4868-4874 ISSN：1533-4880
通讯作者：
Yuan, Yali
作者机构：
[Yuan, Yali; Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Ding, Jianqiang] Zhenhai Ctr Dis Control & Prevent, Ningbo 315200, Peoples R China.;[Xu, Jinsheng] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.;[Guo, Jianbo] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Yuan, Yali] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
会议主办单位：
(1) School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China; (2) Zhenhai Center for Disease Control and Prevention, Ningbo 315200, China; (3) Department of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, China; (4) School of Public Health, University of South China, Hengyang 421001, China
关键词：
ANTIBACTERIAL PROPERTY<&wdkj&>CO-DOPED<&wdkj&>NANOPARTICLE<&wdkj&>TIO2
摘要：
We have prepared a series of TiO<inf>2</inf> nanoparticles for antibacterial applications. These TiO<inf>2</inf> nanoparticles were prepared by the hydrolysis precipitation method with Ti(OBu)<inf>4</inf>, silver nitrate and ammonia. Crystal structure, particle size, interfacial structure and UV-visible light response of the prepared nanoparticles were characterized by X-ray diffraction measurements (XRD), Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR) and UV-Vis diffuse reflectance spectroscopy (UV-Vis-DRs). The XRD spectra showed that all samples were anatase structure calcined at 450 °C for 3 hours. The Ag doping made the peak of diffraction wider. The results of TEM showed that the nanoparticles of TiO <inf>2</inf>, N-TiO<inf>2</inf> and 1% Ag-N-TiO<inf>2</inf> were all spherical in shape and well distributed with a mean size of 19.8 nm, 39.2 nm and 20.7 nm, respectively. N doping caused the nanoparticle size to increase, while, when the doped amount of Ag<sup>+</sup> increased, the TiO<inf>2</inf> particle size decreased. The FTIR revealed that Ag and N doping of TiO<inf>2</inf> appeared to have strong absorption by -OH group and showed the characteristic absorption band of NH<sup>+</sup><inf>4</inf> and Ag. The UV-Vis-DRs indicated that the absorption band of Ag-N co-doped TiO<inf>2</inf> had red shift and that the optical absorption response (between 400 nm and 700 nm) had obvious enhancement. The antibacterial properties of nanoparticles were investigated by agar diffusion method toward Escherichia coli and Bacillus subtilis. The results indicated that both Ag-and N-doped TiO<inf>2</inf> could increase the antibacterial properties of TiO<inf>2</inf> nanoparticles under fluorescent light irradiation. A 1% Ag-N-TiO<inf>2</inf> had the highest antibacterial activity with a clear antibacterial circle of 33.0 mm toward Escherichia coli and 22.8 mm toward Bacillus subtilis after cultivation for 24 hours. Copyright ©2010 American Scientific Publishers.
语种：
英文
展开
Single Nanoparticle to 3D Supercage: Framing for an Artificial Enzyme System
作者：
Cai, Ren;Yang, Dan;Peng, Shengjie;Chen, Xigao;Huang, Yun;Liu, Yuan;Hou, Weijia;Yang, Shengyuan;Liu, Zhenbao;Tan, Weihong*
期刊：
Journal of the American Chemical Society ,2015年137(43):13957-13963 ISSN：0002-7863
通讯作者：
Tan, Weihong
作者机构：
[Yang, Shengyuan; Liu, Yuan; Liu, Zhenbao; Chen, Xigao; Hou, Weijia; Huang, Yun; Cai, Ren; Tan, Weihong] Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA.;[Yang, Shengyuan; Liu, Yuan; Liu, Zhenbao; Chen, Xigao; Hou, Weijia; Huang, Yun; Cai, Ren; Tan, Weihong] Univ Florida, UF Genet Inst, Hlth Canc Ctr, Dept Physiol & Funct Genom, Gainesville, FL 32611 USA.;[Yang, Shengyuan; Liu, Yuan; Liu, Zhenbao; Chen, Xigao; Hou, Weijia; Huang, Yun; Cai, Ren; Tan, Weihong] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA.;[Tan, Weihong] Hunan Univ, Coll Biol, Coll Chem & Chem Engn, Mol Sci & Biomed Lab,State Key Lab Chemo Bio Sen, Changsha 410082, Hunan, Peoples R China.;[Tan, Weihong] Hunan Univ, Collaborat Res Ctr Mol Engn Theranost, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Tan, Weihong] Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA.
摘要：
A facile strategy has been developed to fabricate Cu(OH)<inf>2</inf>supercages (SCs) as an artificial enzyme system with intrinsic peroxidase-mimic activities (PMA). SCs with high catalytic activity and excellent recyclability were generated via direct conversion of amorphous Cu(OH)<inf>2</inf>nanoparticles (NPs) at room temperature. More specifically, the process that takes a single nanoparticle to a 3D supercage involves two basic steps. First, with addition of a copper-ammonia complex, the Cu<sup>2+</sup>ions that are located on the surface of amorphous Cu(OH)<inf>2</inf>NPs would evolve into a fine lamellar structure by coordination and migration and eventually convert to 1D nanoribbons around the NPs. Second, accompanied by the migration of Cu<sup>2+</sup>, a hollow cavity is generated in the inner NPs, such that a single nanoparticle eventually becomes a nanoribbon-assembled 3D hollow cage. These Cu(OH)<inf>2</inf>SCs were then engineered as an artificial enzymatic system with higher efficiency for intrinsic PMA than the peroxidase activity of a natural enzyme, horseradish peroxidase. ©2015 American Chemical Society.
语种：
英文
展开
Silver nanoparticles activate endoplasmic reticulum stress signaling pathway in cell and mouse models: The role in toxicity evaluation
作者：
Huo, Lingling;Chen, Rui;Zhao, Lin;Shi, Xiaofei;Bai, Ru;Long, Dingxin;Chen, Feng;Zhao, Yuliang;Chang, Yan-Zhong;Chen, Chunying*
期刊：
Biomaterials ,2015年61:307-315 ISSN：0142-9612
通讯作者：
Chen, Chunying
作者机构：
[Chang, Yan-Zhong; Huo, Lingling] Hebei Normal Univ, Key Lab Anim Physiol Biochem & Mol Biol Hebei Pro, Key Lab Mol & Cellular Biol, Minist Educ,Coll Life Sci, Shijiazhuang 050016, Peoples R China.;[Shi, Xiaofei; Zhao, Yuliang; Zhao, Lin; Chen, Chunying; Bai, Ru; Chen, Rui; Huo, Lingling] Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China.;[Shi, Xiaofei; Long, Dingxin; Chen, Feng; Zhao, Lin] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Chen, Chunying] Natl Ctr Nanosci & Technol China, 11 Beiyitiao, Beijing 100190, Peoples R China.
通讯机构：
[Chen, Chunying] Natl Ctr Nanosci & Technol China, 11 Beiyitiao, Beijing 100190, Peoples R China.
关键词：
Silver<&wdkj&>Cytotoxicity<&wdkj&>Signaling pathway<&wdkj&>Apoptosis<&wdkj&>Intratracheal instillation
摘要：
Silver nanoparticles (AgNPs) attract considerable public attention both for their antimicrobial properties and their potential adverse effects. In the present study, endoplasmic reticulum (ER) stress was used as a sensitive and early biomarker to evaluate the toxic potential of AgNPs in three different human cell lines in vitro and in vivo in mice. In 16HBE cells, the activation of ER stress signaling pathway was observed by upregulated expression including xbp-1s, chop/DDIT3, TRIB3, ADM2, BIP, Caspase-12, ASNS and HERP at either the mRNA and/or protein levels. However, these changes were not observed in HUVECs or HepG2 cells. Furthermore, mice experiments showed that different tissues had various sensitivities to AgNPs following intratracheal instillation exposure. The lung, liver and kidney showed significant ER stress responses, however, only the lung and kidney exhibited apoptosis by TUNEL assay. The artery and tracheal tissues had lower ER stress and apoptosis after exposure. The lowest observable effect concentrations (LOEC) were proposed based on evaluation of AgNP induced ER stress response in cell and mouse models. In summary, preliminary evaluation of AgNP toxicity by monitoring the ER stress signaling pathway provides new insights toward the understanding the biological impacts of AgNPs. The adverse effects of exposure to AgNPs may be avoided by rational use within the safe dose. (C) 2015 Elsevier Ltd. All rights reserved.
语种：
英文
展开
Label-Free Liquid Crystal Biosensor Based on Specific Oligonucleotide Probes for Heavy Metal Ions
作者：
Yang, Shengyuan;Wu, Chao;Tan, Hui;Wu, Yan;Liao, Shuzhen;Wu, Zhaoyang* ;Shen, Guoli;Yu, Ruqin
期刊：
Analytical Chemistry ,2012年85(1):14-18 ISSN：0003-2700
通讯作者：
Wu, Zhaoyang
作者机构：
[Yang, Shengyuan; Wu, Zhaoyang; Wu, Chao; Wu, Yan; Shen, Guoli; Tan, Hui; Yu, Ruqin; Liao, Shuzhen] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Shengyuan] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Wu, Zhaoyang] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要：
In this study, to enhance the capability of metal ions disturbing the orientation of liquid crystals (LCs), we designed a new label-free LC biosensor for the highly selective and sensitive detection of heavy metal ions. This strategy makes use of the target-induced DNA conformational change to enhance the disruption of target molecules for the orientation of LC leading to an amplified optical signal. The Hg<sup>2+</sup> ion, which possesses a unique property to bind specifically to two DNA thymine (T) bases, is used as a model heavy metal ion. In the presence of Hg<sup>2+</sup>, the specific oligonucleotide probes form a conformational reorganization of the oligonucleotide probes from hairpin structure to duplex-like complexes. The duplex-like complexes are then bound on the triethoxysilylbutyraldehyde/N,N- dimethyl-N-octadecyl (3-aminopropyl) trimethoxysilyl chloride (TEA/DMOAP)-coated substrate modified with capture probes, which can greatly distort the orientational profile of LC, making the optical image of LC cell birefringent as a result. The optical signal of LC sensor has a visible change at the Hg <sup>2+</sup> concentration of low to 0.1 nM, showing good detection sensitivity. The cost-effective LC sensing method can translate the concentration signal of heavy metal ions in solution into the presence of DNA duplexes and is expected to be a sensitive detection platform for heavy metal ions and other small molecule monitors. ©2012 American Chemical Society.
语种：
英文
展开
TiO2 Nanopowder Co-Doped with Iodine and Boron to Enhance Visible-Light Photocatalytic Activity
作者：
Ding, Jianqiang;Yuan, Yali* ;Xu, Jinsheng;Deng, Jian;Guo, Jianbo
期刊：
Journal of Biomedical Nanotechnology ,2009年5(5):521-527 ISSN：1550-7033
通讯作者：
Yuan, Yali
作者机构：
[Ding, Jianqiang; Guo, Jianbo] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Yuan, Yali; Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Xu, Jinsheng] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.
通讯机构：
[Yuan, Yali] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
会议名称：
3rd International Symposium on Biomedical Engineering, Bioanalysis and Nanotechnology
会议时间：
JUN 08-10, 2008
会议地点：
Hunan Univ, Dept Sci & Technol, Hunan, PEOPLES R CHINA
会议主办单位：
[Yuan, Yali;Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.^[Ding, Jianqiang;Guo, Jianbo] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.^[Xu, Jinsheng] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.
关键词：
BORON<&wdkj&>IODINE<&wdkj&>METHYL ORANGE<&wdkj&>PHOTOCATALYTIC ACTIVITY<&wdkj&>TIO2<&wdkj&>VISIBLE-LIGHT
摘要：
An iodine and boron co-doped TiO<inf>2</inf> photocatalyst was prepared by the hydrolyzation-precipitation method. X-ray diffraction (XRD), ultraviolet-visible diffuse reflectance spectroscopy (UV-Vis DRS), and X-ray photoelectron spectroscopy (XPS) were applied to characterize the crystalline structure, light absorbing ability, and the chemical state of iodine and boron in the photocatalysts. The results of photocatalytic degradation of methyl orange demonstrated that the I-B-TiO<inf>2</inf> catalyst prepared at 400 °C for 3 h exhibited the highest photocatalytic activity with a methyl orange degradation ratio of 61% under visible-light (λ&le 420 nm) irradiation for 120 min. The characterization results revealed that I-B-TiO<inf>2</inf> is in conformity with the anatase TiO<inf>2</inf> and that the doping of iodine and boron ions could efficiently inhibit the grain growth. Doped iodine was present in the multivalent forms of I<sup>7+</sup>, I<sup>-</sup> and I<sup>5+</sup>. Doped boron was present as B<sup>3+</sup> in an as-prepared sample, forming a possible chemical environment such as B-O-Ti. Overall, the doping of I and B enhanced the ability of TiO<inf>2</inf> to absorb visible-light, and it was observed that the photocatalytic activity of I-B-TiO<inf>2</inf> was enhanced by the synergistic effect of I and B. Copyright ©2009 American Scientific Publishers All rights reserved.
语种：
英文
展开
Carbon paste electrode modified with duplex molecularly imprinted polymer hybrid film for metronidazole detection
作者：
Xiao, Ni;Deng, Jian* ;Cheng, Jianlin;Ju, Saiqin;Zhao, Haiqing;Xie, Jin;Qian, Duo;He, Jun
期刊：
Biosensors and Bioelectronics ,2016年81:54-60 ISSN：0956-5663
通讯作者：
Deng, Jian
作者机构：
[Xiao, Ni; Zhao, Haiqing; He, Jun; Deng, Jian; Qian, Duo; Ju, Saiqin; Xie, Jin] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Cheng, Jianlin] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Deng, Jian] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Metronidazole<&wdkj&>Electropolymerization<&wdkj&>Anilinomethyltriethoxysilane<&wdkj&>Duplex molecularly imprinted polymer<&wdkj&>Electrochemical sensor
摘要：
A novel electrochemical sensor based on duplex molecularly imprinted polymer (DMIP) hybrid film modified carbon paste electrode (CPE) has been developed for highly sensitive and selective determination of metronidazole (MNZ). A conductive poly(anilinomethyltriethoxysilane) film is firstly electrodeposited on the surface of a CPE, and then a molecularly imprinted polysiloxane (MIPS) membrane is covalently covered on the film via sol-gel process. The as-constructed DMIP hybrid film, combining the advantages of MIPS and conducting MIP, can make feasible the direct and efficient signal transformation between the target analyte and the transducer, as well as enhance the imprinting recognition capability, mass transfer efficiency and the detection sensitivity. Under optimized conditions, the reduction peak currents of MNZ are linear to MNZ concentrations in the range from 4.0×10<sup>-7</sup>to 2.0×10<sup>-4</sup>mol L<sup>-1</sup>with a detection limit of 9.1×10<sup>-8</sup>mol L<sup>-1</sup>. The RSD values vary from 2.9% to 4.7% for intra-day and from 3.4% to 4.2% for inter-day precision. The DMIP-based sensor has been successfully applied for the determination of MNZ in biological and pharmaceutical samples. The accuracy and reliability of the method is further confirmed by high performance liquid chromatography. ©2016 Elsevier B.V.
语种：
英文
展开
Synchronous fluorescence determination of urinary 1-hydroxypyrene, β-naphthol and 9-hydroxyphenanthrene based on the sensitizing effect of β-cyclodextrin
作者：
Yang, Hong-Mei;Wang, Yong-Sheng* ;Li, Jun-Hong;Li, Gui-Rong;Wang, Ying;Tan, Xuan;Xue, Jin-Hua;Mao, Xi-Lin;Kang, Rong-Hui
期刊：
Analytica Chimica Acta ,2009年636(1):51-57 ISSN：0003-2670
通讯作者：
Wang, Yong-Sheng
作者机构：
[Li, Jun-Hong; Kang, Rong-Hui; Wang, Ying; Tan, Xuan; Li, Gui-Rong; Yang, Hong-Mei; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Mao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Synchronous fluorescence<&wdkj&>1-Hydroxypyrene<&wdkj&>β-Naphthol<&wdkj&>9-Hydroxyphenanthrene<&wdkj&>β-Cyclodextrin<&wdkj&>Urine
摘要：
A novel method for the simultaneous determination of 1-hydroxypyrene (1-OHP), β-naphthol (β-NAP) and 9-hydroxyphenanthrene (9-OHPe) in human urine has been established by using synchronous fluorescence spectrometry. It was based on the fact that synchronous fluorescence spectrometry can resolve the broad-band overlapping of conventional fluorescence spectra, which arise from their similar molecular structures. Only one single scan is needed for quantitative determination of three compounds simultaneously when Δλ= 15 nm is chosen. The signals detected at these three wavelengths, 369.6, 330.0 and 358.0 nm, vary linearly when the concentration of 1-OHP, β-NAP and 9-OHPe is in the range of 2.16 ×10<sup>-8</sup>-1.50 ×10<sup>-5</sup> mol L<sup>-1</sup>, 1.20 ×10<sup>-7</sup>-1.10 ×10<sup>-5</sup> mol L<sup>-1</sup> and 1.07 ×10<sup>-7</sup>-3.50 ×10<sup>-5</sup> mol L<sup>-1</sup>, respectively. The correlation coefficients for the standard calibration graphs were 0.994, 0.999 and 0.997 (n = 7) for 1-OHP, β-NAP and 9-OHPe, respectively. The limits of detection (LOD) for 1-OHP, β-NAP and 9-OHPe were 6.47 ×10<sup>-9</sup> mol L<sup>-1</sup>, 3.60 ×10<sup>-8</sup> mol L<sup>-1</sup> and 3.02 ×10<sup>-8</sup> mol L<sup>-1</sup>with relative standard deviations (R.S.D.) of 4.70-6.40%, 2.80-4.20%, 3.10-4.90% (n = 6), respectively. The method described here had been applied to determine traces of 1-OHP, β-NAP and 9-OHPe in human urine, and the obtained results were in good agreement with those obtained by the HPLC method. In addition, the interaction modes between β-cyclodextrin (β-CD) and 1-OHP, β-NAP or 9-OHPe, as well as the mechanism of the fluorescence enhancement were also discussed. ©2009 Elsevier B.V. All rights reserved.
语种：
英文
展开
Resonance light scattering determination of uranyl based on labeled DNAzyme-gold nanoparticle system
作者：
Zhou, Bin;Shi, Lin-Fei;Wang, Yong-Sheng;Yang, Hui-Xian;Xue, Jin-Hua;Liu, Lu;Wang, Yong-Song* ;Yin, Ji-Cheng;Wang, Jia-Cheng
期刊：
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy ,2013年110:419-424 ISSN：1386-1425
通讯作者：
Wang, Yong-Song
作者机构：
[Zhou, Bin; Wang, Yong-Song; Yang, Hui-Xian; Yin, Ji-Cheng; Shi, Lin-Fei; Xue, Jin-Hua; Wang, Yong-Sheng; Liu, Lu; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 427001, Peoples R China.
通讯机构：
[Wang, Yong-Song] Univ South China, Coll Publ Hlth, Hengyang 427001, Peoples R China.
关键词：
Resonance light scattering<&wdkj&>DNAzyme<&wdkj&>Gold nanoparticles<&wdkj&>Uranyl
摘要：
A resonance light scattering (RLS) method has been developed using a uranyl (UO22+) specific DNAzyme and gold nanoparticles (AuNPs). In this strategy, the cleavage of the substrate strand (SDNA) of DNAzyme results in releasing a shorter duplex in the presence of UO22+, leading to the aggregation of AuNPs and the increase of RLS intensity. The response signals linearly correlated with the concentration of UO22+ over the range of 1.36 ×10<sup>-8</sup>-1.50 ×10<sup>-7</sup> mol L<sup>-1</sup>. The limit of detection (LOD) is 4.09 ×10<sup>-9</sup> mol L<sup>-1</sup>. The method has excellent selectivity and higher sensitivity. It could provide a promising potential for the detection of metal ions, and be benefit to extend the application of RLS method. ©2013 Elsevier B.V. All rights reserved.
语种：
英文
展开
Adsorption and recovery of U(vi) from low concentration uranium solution by amidoxime modified Aspergillus niger
作者：
Li, Le;Hu, Nan;Ding, Dexin* ;Xin, Xin;Wang, Yongdong;Xue, Jinhua;Zhang, Hui;Tan, Yan
期刊：
RSC Advances ,2015年5(81):65827-65839 ISSN：2046-2069
通讯作者：
Ding, Dexin
作者机构：
[Ding, Dexin; Zhang, Hui; Tan, Yan; Li, Le; Hu, Nan; Xin, Xin; Xue, Jinhua; Wang, Yongdong] Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang 421001, Peoples R China.;[Tan, Yan; Li, Le; Xue, Jinhua] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Ding, Dexin] Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang 421001, Peoples R China.
摘要：
Amidoxime modified Aspergillus niger (AMAN) was prepared by the oximation reaction. The effects of the initial pH, contact time, initial U(vi) concentration and biosorbent dose on the adsorption of U(vi) ions from radioactive wastewater in U(vi) concentrations of less than 1 mg L<sup>-1</sup>by AMAN and the raw Aspergillus niger (RAN) were investigated. The maximum adsorption efficiency by AMAN for the 0.5 mg L<sup>-1</sup>U(vi) solution amounted to 98.85% under the optimum adsorption conditions, while the maximum adsorption efficiency by RAN was only 77.83%. The adsorption equilibrium data were found to be best fitted to Langmuir isotherm model, and the maximum biosorption capacity of AMAN for U(vi) was estimated to be 621 mg g<sup>-1</sup>at 298 K. The biosorption kinetics followed the pseudo-second order model and intraparticle diffusion equation. The Gibbs free energy change (ΔG°), enthalpy change (ΔH°) and entropy change (ΔS°) showed that the adsorption process of U(vi) was spontaneous, feasible and endothermic. The SEM-EDS study indicated that much more U(vi) ions were adsorbed by AMAN than by RAN. FT-IR study showed that the -NH<inf>2</inf>and =N-OH groups of amidoxime were the dominant ones for binding UO<inf>2</inf><sup>2+</sup>ions. Moreover, AMAN was found to have excellent selective adsorption capability of U(vi) due to amidoxime groups. The UO<inf>2</inf><sup>2+</sup>ions adsorbed by AMAN could be desorbed using 0.1 M HCl, and the desorption efficiency reaching 87.28% at the 8th cycle of adsorption and desorption. ©The Royal Society of Chemistry.
语种：
英文
展开
Rapid simultaneous analysis of 1-hydroxypyrene, 2-hydroxyfluorene, 9-hydroxyphenanthrene, 1- and 2-naphthol in urine by first derivative synchronous fluorescence spectrometry using Tween-20 as a sensitizer
作者：
Kang, Rong-Hui;Wang, Yong-Sheng* ;Yang, Hong-Mei;Li, Gui-Rong;Tan, Man;Xue, Jin-Hua;Zhang, Jin-Quan;Yuan, Yu-Kun;Shi, Lin-Fei;Xiao, Xi-Lin
期刊：
Analytica Chimica Acta ,2010年658(2):180-186 ISSN：0003-2670
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Tan, Man; Shi, Lin-Fei; Kang, Rong-Hui; Li, Gui-Rong; Zhang, Jin-Quan; Yang, Hong-Mei; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Derivative synchronous fluorescence;Polycyclic aromatic hydrocarbons;Tween-20;Urine
摘要：
A novel method of first derivative synchronous fluorescence was developed for the rapid simultaneous analysis of trace 1-hydroxypyrene (1-OHP), 1-naphthol (1-NAP), 2-naphthol (2-NAP), 9-hydroxyphenanthrene (9-OHPe) and 2-hydroxyfluorene (2-OHFlu) in human urine. Only one single scan was needed for quantitative determination of five compounds simultaneously when Δλ= 10 nm was chosen. In the optimal experimental conditions, there was a linear relationship between the fluorescence intensity and the concentration of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in the range of 1.75 ×10<sup>-9</sup>to 4.50 ×10<sup>-6</sup>mol L<sup>-1</sup>, 3.64 ×10<sup>-8</sup>to 2.20 ×10<sup>-4</sup>mol L<sup>-1</sup>, 8.18 ×10<sup>-9</sup>to 1.20 ×10<sup>-4</sup>mol L<sup>-1</sup>, 3.26 ×10<sup>-9</sup>to 8.50 ×10<sup>-5</sup>mol L<sup>-1</sup>and 4.88 ×10<sup>-9</sup>to 5.50 ×10<sup>-6</sup>mol L<sup>-1</sup>, respectively. The limits of detection (LOD) were found to be 5.25 ×10<sup>-10</sup>mol L<sup>-1</sup>for 1-OHP, 1.10 ×10<sup>-8</sup>mol L<sup>-1</sup>for 1-NAP, 2.46 ×10<sup>-9</sup>mol L<sup>-1</sup>for 2-NAP, 9.77 ×10<sup>-10</sup>mol L<sup>-1</sup>for 9-OHPe and 1.46 ×10<sup>-9</sup>mol L<sup>-1</sup>for 2-OHFlu. The proposed method is reliable, selective and sensitive, and has been used successfully in the determination of traces of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in human urine samples, whose results were in good agreement with those gained by the HPLC method. ©2009 Elsevier B.V. All rights reserved.
语种：
英文
展开
Urinary metal concentrations in relation to semen quality: A cross-sectional study in China
作者：
Zeng, Qiang;Feng, Wei;Zhou, Bin;Wang, Yi-Xin;He, Xiao-Sheng;Yang, Pan;You, Ling;Yue, Jing;Li, Yu-Feng;Lu, Wen-Qing*
期刊：
Environmental Science and Technology ,2015年49(8):5052-5059 ISSN：0013-936X
通讯作者：
Lu, Wen-Qing
作者机构：
[Wang, Yi-Xin; Yang, Pan; Feng, Wei; Lu, Wen-Qing; You, Ling; Zeng, Qiang; He, Xiao-Sheng] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Dept Occupat & Environm Hlth, Wuhan 430030, Hubei, Peoples R China.;[Wang, Yi-Xin; Yang, Pan; Feng, Wei; Lu, Wen-Qing; You, Ling; Zeng, Qiang; He, Xiao-Sheng] Huazhong Univ Sci & Technol, Sch Publ Hlth, Tongji Med Coll, Key Lab Environm & Hlth,Minist Educ, Wuhan 430030, Hubei, Peoples R China.;[Wang, Yi-Xin; Yang, Pan; Feng, Wei; Lu, Wen-Qing; You, Ling; Zeng, Qiang; He, Xiao-Sheng] Huazhong Univ Sci & Technol, Sch Publ Hlth, Tongji Med Coll, Minist Environm Protect, Wuhan 430030, Hubei, Peoples R China.;[Wang, Yi-Xin; Yang, Pan; Feng, Wei; Lu, Wen-Qing; You, Ling; Zeng, Qiang; He, Xiao-Sheng] Huazhong Univ Sci & Technol, Sch Publ Hlth, Tongji Med Coll, State Key Lab Environm Hlth Incubating, Wuhan 430030, Hubei, Peoples R China.;[Zhou, Bin] Univ South China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Lu, Wen-Qing] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Dept Occupat & Environm Hlth, Wuhan 430030, Hubei, Peoples R China.
摘要：
Exposure to metals, including essential and nonessential elements, is widespread and may be associated with altered semen quality. This study aimed to examine the association between urinary metal concentrations and semen quality in a Chinese population. We measured semen quality parameters (sperm concentration, count, motility, normal morphology, and abnormal head) and 13 metals [arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), iron (Fe), lead (Pb), manganese (Mn), molybdenum (Mo), mercury (Hg), nickel (Ni), selenium (Se), and zinc (Zn)] in the urine of 394 men from an infertility clinic. Multivariable logistic and linear regressions were used to assess the relationship between the creatinine-adjusted urinary metal concentrations and semen quality parameters. We found a significant trend for decreased odds ratios (ORs) for below-reference sperm count with increasing Se quartiles (p for trend = 0.04) and a significant trend for increased sperm percent abnormal head with increasing Ni quartiles (p for trend = 0.03). These associations persisted, even when considering multiple metals. Our results suggest that Ni exposure may be associated with deteriorated sperm morphology and that Se exposure may be associated with better semen quality. However, our findings warrant further studies in a larger and general population. ©2015 American Chemical Society.
语种：
英文
展开
Removal of aqueous uranyl ions by magnetic functionalized carboxymethylcellulose and adsorption property investigation
作者：
Gao, Yangyang;Yuan, Yali* ;Ma, Dandan;Li, Le;Li, Yuhui;Xu, Wenhui;Tao, Wei
期刊：
Journal of Nuclear Materials ,2014年453(1-3):82-90 ISSN：0022-3115
通讯作者：
Yuan, Yali
作者机构：
[Gao, Yangyang; Tao, Wei; Yuan, Yali; Xu, Wenhui; Li, Yuhui; Ma, Dandan] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Li, Le] Univ South China, Dept Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Yuan, Yali] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
摘要：
Magnetic carboxymethylcellulose (CMC/Fe3O4) was used as a framework adsorption material to remove uranium ions from aqueous solutions. Carboxyl functional groups were grafted onto the CMC/Fe3O4. The maximum adsorption capacity of the magnetic composite toward U(VI) was 122.48 mg/g. Kinetic and thermodynamic parameters of the adsorption process were estimated. The pseudo-second-order model was more suitable and it proved to be an endothermic and spontaneous process. The Langmuir and Freundlich models were applied to evaluate the adsorption isotherm. The data matched well with Langmuir model after equilibrium was reached and with Freundlich model before equilibrium was reached.
语种：
英文
展开
Removal and Recovery of U(VI) from Low Concentration Radioactive Wastewater by Ethylenediamine-Modified Biomass of Aspergillus niger
作者：
Ding, De Xin* ;Xin, Xin;Li, Le;Hu, Nan;Li, Guang Yue;Wang, Yong Dong;Fu, Ping Kun
期刊：
Water, Air, and Soil Pollution ,2014年225(12):2206- ISSN：0049-6979
通讯作者：
Ding, De Xin
作者机构：
[Wang, Yong Dong; Li, Guang Yue; Ding, De Xin; Hu, Nan; Xin, Xin; Fu, Ping Kun] Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang 421001, Peoples R China.;[Li, Le] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Ding, De Xin] Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang 421001, Peoples R China.
关键词：
Aspergillus niger<&wdkj&>Ethylenediamine<&wdkj&>Biosorption<&wdkj&>Low concentration radioactive wastewater<&wdkj&>U(VI)
摘要：
In order to develop an effective and economical method for removing U(VI) from the low concentration radioactive wastewater with the U(VI) concentration of less than 1 mg L<sup>-1</sup>, the biomass of Aspergillus niger was prepared and modified with ethylenediamine, and the biosorption of uranium from the low concentration radioactive wastewater by the unmodified and the modified biomasses was investigated in a batch system. The modified biomass exhibited the adsorption efficiency of 99.25 % for uranium under the optimum conditions that pH was 5.0, the contact time was 150 min, and the biosorbent dose was 0.2 g L<sup>-1</sup>. The adsorption fitted well to Langmuir isotherm, and the maximum sorption capacity of the modified biomass for U(VI) was determined to be 6.789 mg g<sup>-1</sup> which increased by 36.45 % compared with the unmodified biomass. The adsorption kinetics was better depicted by pseudo-second-order kinetic model. The Gibbs free energy change (ΔG <sup>0</sup>), enthalpy change (ΔH <sup>0</sup>), and entropy change (ΔS <sup>0</sup>) showed that the process of U(VI) adsorption was spontaneous, endothermic, and feasible. The changes in the groups, morphology, and the presence of U(VI) on the surface of the adsorbents which were characterized by FT-IR, SEM, and EDS, demonstrated that the U(VI) was successfully adsorbed onto the modified biomass. Moreover, the UO<inf>2</inf> <sup>2+</sup> absorbed on the modified biomass can be released by 0.1 mol L<sup>-1</sup> HNO<inf>3</inf> with high desorption efficiency of 99.21 %. The results show that the modified biomass can remove U(VI) from low concentration radioactive wastewater more effectively than the unmodified biomass.
语种：
英文
展开
Dietary exposure to endocrine disrupting chemicals in metropolitan population from China: A risk assessment based on probabilistic approach
作者：
He, Dongliang;Ye, Xiaolei;Xiao, Yonghua;Zhao, Nana;Long, Jia;Zhang, Piwei;Fan, Ying;Ding, Shibin;Jin, Xin;Tian, Chong;Xu, Shunqing;Ying, Chenjiang*
期刊：
Chemosphere ,2015年139:2-8 ISSN：0045-6535
通讯作者：
Ying, Chenjiang
作者机构：
[Zhang, Piwei; Ying, Chenjiang; He, Dongliang; Fan, Ying; Ding, Shibin; Tian, Chong; Jin, Xin; Long, Jia] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Nutr & Food Hyg, Hubei Key Lab Food Nutr & Safety,Sch Publ Hlth, Wuhan 430030, Peoples R China.;[Ye, Xiaolei; Zhao, Nana] Wenzhou Med Univ, Sch Environm Sci & Publ Hlth, Wenzhou 325000, Peoples R China.;[Xiao, Yonghua] Wuhan Ctr Dis Control & Prevent, Wuhan 430015, Peoples R China.;[Xu, Shunqing] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Minist Educ,Key Lab Environm & Hlth, Wuhan 430030, Peoples R China.;[He, Dongliang] Univ South China, Sch Publ Hlth, Dept Prevent Med, Hengyang 421001, Peoples R China.
通讯机构：
[Ying, Chenjiang] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Nutr & Food Hyg, Hubei Key Lab Food Nutr & Safety,Sch Publ Hlth, Wuhan 430030, Peoples R China.
关键词：
17beta-Estradiol equivalent ( summation operatorEEQs);Dietary exposure;Endocrine disrupting chemicals (EDCs);Probabilistic modeling;Risk assessment
摘要：
The intake of contaminated foods is an important exposure pathway for endocrine disrupting chemicals (EDCs). However, data on the occurrence of EDCs in foodstuffs are sporadic and the resultant risk of co-exposure is rarely concerned. In this study, 450 food samples representing 7 food categories (mainly raw and fresh food), collected from three geographic cities in China, were analyzed for eight EDCs using high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Besides estrone (E<inf>1</inf>), other EDCs including diethylstilbestrol (DES), nonylphenol (NP), bisphenol A (BPA), octylphenol (OP), 17β-estradiol (E<inf>2</inf>), 17α-ethinylestradiol (EE<inf>2</inf>), and estriol (E<inf>3</inf>) were ubiquitous in food. Dose-dependent relationships were found between NP and EE<inf>2</inf>(r=0.196, p<0.05), BPA (r=0.391, p<0.05). Moreover, there existed a correspondencebetween EDCs congener and food category. Based on the obtained database of EDCs concentration combined with local food consumption, dietary EDCs exposure was estimated using the Monte Carlo Risk Assessment (MCRA) system. The 50th and 95th percentile exposure of any EDCs isomer were far below the tolerable daily intake (TDI) value identically. However, the sum of 17β-estradiol equivalents (∑EEQs) exposure in population was considerably larger than the value of exposure to E<inf>2</inf>, which implied the underlying resultant risk of multiple EDCs in food should be concern. In conclusion, co-exposure via food consumption should be considered rather than individual EDCs during health risk evaluation.<br/> ©2015 Elsevier Ltd.
语种：
英文
展开
Ultrasensitive detection of uranyl by graphene oxide-based background reduction and RCDzyme-based enzyme strand recycling signal amplification
作者：
Li, Ming-Hui;Wang, Yong-Sheng* ;Cao, Jin-Xiu;Chen, Si-Han;Tang, Xian;Zhu, Xiao-Feng;Zhu, Yu-Feng;Huang, Yan-Qin
期刊：
Biosensors and Bioelectronics ,2015年72:294-299 ISSN：0956-5663
通讯作者：
Wang, Yong-Sheng
作者机构：
[Chen, Si-Han; Li, Ming-Hui; Tang, Xian; Cao, Jin-Xiu; Zhu, Yu-Feng; Wang, Yong-Sheng; Huang, Yan-Qin; Zhu, Xiao-Feng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
RNA-cleaving chimera DNAzyme<&wdkj&>Enzyme strand recycling amplification<&wdkj&>G-quadruplexes<&wdkj&>Graphene oxide<&wdkj&>N-methyl- mesoporphyrin IX<&wdkj&>Zero-background signal
摘要：
We proposed a novel strategy which combines graphene oxide-based background reduction with RCDzyme-based enzyme strand recycling amplification for ultrahigh sensitive detection of uranyl. The RCDzyme is designed to contain a guanine (G)-rich sequence that replaces the partial sequence in an uranyl-specific DNAzyme. This multifunctional probe can act as the target recognition element, DNAzyme and the primer of signal amplification. The presence of UO2(2+) can induce the cleavage of the substrate strands in RCDzyme. Then, each released enzyme strand can hybridize with another substrate strands to trigger many cycles of the cleavage by binding uranyl, leading to the formation of more G-quadruplexes by split guanine-rich oligonucleotide fragments. The resulting G-quadruplexes could bind to N-methyl-mesoporphyrin IX (NMM), causing an amplified detection signal for the target uranyl. Next, graphene oxide-based background reduction strategy was further employed for adsorbing free ssDNA and NMM, thereby providing a proximalis zero-background signal. The combination of RCDzyme signal amplification and proximalis zero-background signal remarkably improves the sensitivity of this method, achieving a dynamic range of two orders of magnitude and giving a detection limit down to 86pM, which is much lower than those of related literature reports. These achievements might be helpful in the design of highly sensitive analytical platform for wide applications in environmental and biomedical fields. Copyright © 2015 Elsevier B.V. All rights reserved.
语种：
英文
展开
Determination of formaldehyde in aquatic products by a sensitive catalytic fluorescence method
作者：
Li, Guirong* ;Han, Lijuan
期刊：
Analytical Methods ,2014年6(2):426-432 ISSN：1759-9660
通讯作者：
Li, Guirong
作者机构：
[Li, Guirong; Han, Lijuan] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Guirong] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
A novel method for the determination of trace formaldehyde in food samples has been established using a catalytic kinetic fluorimetry technique. It is based on the fact that the oxidation of pyronine Y by sodium periodate is catalyzed by formaldehyde in phosphoric acid. When the excitation wavelength was set at 546 nm, it was observed that decreased intensity (ΔF) of fluorescence at 563 nm was proportional to the concentration of formaldehyde in the range 0 to 0.30 μg mL<sup>-1</sup>. The limit of detection and the average recovery for formaldehyde were 2.0 ×10<sup>-2</sup> ng mL <sup>-1</sup> and 101.3% (n = 6), respectively. The present method has been applied to the determination of trace formaldehyde in aqueous products and the results observed were in good agreement with those obtained by distillation-spectrophotometry. A review of recently published catalytic or inhibiting kinetic methods for the determination of HCHO is presented for comparison. The mechanism of reaction is studied. This journal is ©2014 The Royal Society of Chemistry.
语种：
英文
展开
Determination of leucomalachite green, leucocrystal violet and their chromic forms using excitation-emission matrix fluorescence coupled with second-order calibration after dispersive liquid-liquid microextraction
作者：
Ju, Saiqin;Deng, Jian* ;Cheng, Jianlin;Xiao, Ni;Huang, Kaihui;Hu, Canhui;Zhao, Haiqing;Xie, Jin;Zhan, Xiaozhu
期刊：
Food Chemistry ,2015年185:479-487 ISSN：0308-8146
通讯作者：
Deng, Jian
作者机构：
[Xiao, Ni; Huang, Kaihui; Hu, Canhui; Zhao, Haiqing; Deng, Jian; Ju, Saiqin; Xie, Jin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Cheng, Jianlin] Univ South China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Zhan, Xiaozhu] Sanming Supervis & Testing Ctr Qual & Safety Agro, Sanming 365000, Fujian, Peoples R China.
通讯机构：
[Deng, Jian] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Leucomalachite green<&wdkj&>Leucocrystal violet<&wdkj&>Malachite green<&wdkj&>Crystal violet<&wdkj&>Dispersive liquid–liquid microextraction<&wdkj&>Parallel factor analysis<&wdkj&>Alternating trilinear decomposition
摘要：
A novel spectrofluorimetric method has been developed for the simultaneous determination of leucomalachite green (LMG), leucocrystal violet (LCV), malachite green (MG) and crystal violet (CV) by combining the sensitivity of molecular fluorescence and the selectivity of the second-order calibration. Residues of LMG, LCV, MG and CV were simultaneously extracted from fish and shrimp muscle with acetonitrile. The non-fluorescent CV and MG were then reduced to the corresponding fluorescent LMG and LCV by reacting with sodium borohydride. After preconcentration with dispersive liquid-liquid microextraction technique, the extracts were analyzed by using excitation-emission matrix fluorescence coupled with second-order calibration methods based on parallel factor analysis (PARAFAC) and alternating trilinear decomposition (ATLD) algorithms. The limits of detection obtained were 2.21-2.65 ng g<sup>-1</sup>by PARAFAC and 2.30-2.86 ng g<sup>-1</sup>by ATLD, respectively. The developed method was successfully applied to simultaneous determination of the four analytes in grass carp and shrimp samples with recoveries of 90.53-103.03% for PARAFAC and 90.40-102.75% for ATLD. The accuracy of this novel method was also verified by high performance liquid chromatography. ©2015 Elsevier Ltd.
语种：
英文
展开
PM2.5 induced apoptosis in endothelial cell through the activation of the p53-bax-caspase pathway
作者：
Wang, Wuxiang;Deng, Ziyong;Feng, Yuqin;Liao, Fen;Zhou, Furong;Feng, Shaolong* ;Wang, Xinming*
期刊：
Chemosphere ,2017年177:135-143 ISSN：0045-6535
通讯作者：
Feng, Shaolong;Wang, Xinming
作者机构：
[Feng, Shaolong; Wang, Wuxiang; Deng, Ziyong; Zhou, Furong; Liao, Fen] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Wang, Wuxiang] Univ South China, Lib, Hengyang 421001, Peoples R China.;[Feng, Yuqin] Jilin Univ, Coll Mat Sci & Engn, Changchun 130022, Peoples R China.;[Wang, Xinming] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China.
通讯机构：
[Feng, Shaolong] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Wang, Xinming] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China.
关键词：
Fine particulate matter (PM2.5)<&wdkj&>Endothelial cell (EC)<&wdkj&>Apoptosis<&wdkj&>p53<&wdkj&>Guangzhou
摘要：
Exposure to airborne fine particulate matter (PM<inf>2.5</inf>) is associated with cardiovascular diseases (CVDs). Nevertheless, a comprehensive understanding of the underlying biological mechanisms by which PM<inf>2.5</inf>exposure induces or aggravates CVDs remain insufficiently clear. In the present study, the flow cytometry was employed to investigate the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by PM<inf>2.5</inf>in culture. The underlying apoptotic pathway was also studied through the determination of the protein expression and activation of p53, Bax, Bcl-2, caspases-9, -7, -3, and PARP by western blot. The results showed that PM<inf>2.5</inf>could significantly induce the apoptosis of HUVECs at the tested concentrations (0.2, 1, 5, 25 μg mL<sup>−1</sup>), compared with the negative control (p < 0.05, p < 0.01). The apoptotic rate of HUVECs increased with the elevating levels of PM<inf>2.5</inf>exposure, showing a clear dose-effect relationship. Moreover, the increasing phosphorylation of p53, decreasing ratio of Bcl-2/Bax, and enhancing activation of the downstream proteins caspase-9, -7, -3 and PARP, were also observed with the increasing concentrations of PM<inf>2.5</inf>administration in the western blot, indicating that the intracellular approach of apoptosis, the p53-Bax-caspases pathway, is the major way of PM<inf>2.5</inf>-induced apoptosis in HUVECs. In conclusion, these results suggested that induction of EC apoptosis is an important mechanism by which ambient PM<inf>2.5</inf>exposure poses adverse effects on the cardiovascular system. ©2017 Elsevier Ltd
语种：
英文
展开
An ultrasensitive label-free assay of 8-hydroxy-2'-deoxyguanosine based on the conformational switching of aptamer
作者：
Wang, Jia-Cheng;Wang, Yong-Sheng;Xue, Jin-Hua;Zhou, Bin;Qian, Qiu-Mei;Wang, Yong-Song* ;Yin, Ji-Cheng;Zhao, Hui;Liu, Hui;Liu, Shan-Du
期刊：
Biosensors and Bioelectronics ,2014年58:22-26 ISSN：0956-5663
通讯作者：
Wang, Yong-Song
作者机构：
[Zhou, Bin; Wang, Yong-Song; Yin, Ji-Cheng; Qian, Qiu-Mei; Zhao, Hui; Liu, Hui; Liu, Shan-Du; Xue, Jin-Hua; Wang, Yong-Sheng; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Wang, Jia-Cheng] Dali Univ, Coll Publ Hlth, Dali 671000, Peoples R China.
通讯机构：
[Wang, Yong-Song] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Ultrasensitive assay<&wdkj&>Aptamer<&wdkj&>Gold nanoparticles<&wdkj&>8-hydroxy-2′-deoxyguanosine<&wdkj&>Conformational switching<&wdkj&>Resonance light scattering
摘要：
We developed a highly sensitive label-free assay of 8-hydroxy-2 '-deoxyguanosine (8-OHdG) using 8-OHdG-aptamer (Apt) as the recognition element. The Apt was adsorbed onto the surface of gold nanoparticles (AuNPs), which prevents them from aggregating under high-salt conditions. Upon addition of 8-OHdG, the conformation of the Apt changes to form a G-quadruplex structure, which leads to the aggregation of the AuNPs along with the increase of the resonance light scattering intensity. The mechanism of 8-OHdG that induces Apt to form G-quadruplexes structure was studied by circular dichroism. The response signals linearly correlated with the concentration of 8-OHdG ranging from 90.8 pM to 14.1 nM with a detection limit of 27.3 pM, which is much lower than that obtained by other methods. This method does not need any label steps and sophisticated equipment. The application for detection of 8-OHdG in real samples further demonstrated its reliability. This strategy would be helpful for developing a universal analytical method by simply replacing corresponding aptamers for various target molecules. (C) 2014 Elsevier B.V. All rights reserved.
语种：
英文
展开
